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P00723

- BGAL_KLULA

UniProt

P00723 - BGAL_KLULA

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Protein

Beta-galactosidase

Gene

LAC4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei482 – 4821Proton donorBy similarity
Active sitei551 – 5511NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 2826.
SABIO-RKP00723.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:LAC4
Ordered Locus Names:KLLA0B14883g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome B

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidasePRO_0000057663Add
BLAST

Proteomic databases

PRIDEiP00723.

Interactioni

Protein-protein interaction databases

STRINGi28985.P00723.

Structurei

Secondary structure

1
1025
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Beta strandi16 – 183Combined sources
Turni29 – 313Combined sources
Beta strandi32 – 343Combined sources
Beta strandi37 – 4610Combined sources
Helixi47 – 493Combined sources
Helixi53 – 553Combined sources
Helixi58 – 614Combined sources
Beta strandi66 – 705Combined sources
Helixi77 – 793Combined sources
Beta strandi85 – 906Combined sources
Beta strandi106 – 11510Combined sources
Helixi117 – 1226Combined sources
Beta strandi123 – 1308Combined sources
Beta strandi132 – 1409Combined sources
Beta strandi143 – 1497Combined sources
Beta strandi155 – 1584Combined sources
Turni160 – 1623Combined sources
Beta strandi165 – 17612Combined sources
Helixi179 – 1835Combined sources
Beta strandi187 – 1904Combined sources
Beta strandi198 – 22023Combined sources
Beta strandi225 – 23713Combined sources
Beta strandi241 – 2477Combined sources
Helixi258 – 2625Combined sources
Beta strandi275 – 2806Combined sources
Beta strandi287 – 2959Combined sources
Beta strandi301 – 3044Combined sources
Beta strandi306 – 3149Combined sources
Beta strandi320 – 3289Combined sources
Beta strandi333 – 3364Combined sources
Beta strandi339 – 3424Combined sources
Beta strandi349 – 3535Combined sources
Turni359 – 3613Combined sources
Helixi367 – 37913Combined sources
Beta strandi384 – 3863Combined sources
Helixi396 – 4038Combined sources
Beta strandi406 – 4105Combined sources
Helixi416 – 4194Combined sources
Helixi420 – 4256Combined sources
Turni433 – 4364Combined sources
Helixi437 – 4404Combined sources
Turni441 – 4433Combined sources
Helixi444 – 4463Combined sources
Turni447 – 4493Combined sources
Helixi451 – 4533Combined sources
Helixi454 – 46815Combined sources
Beta strandi474 – 4785Combined sources
Helixi487 – 49913Combined sources
Beta strandi503 – 5064Combined sources
Beta strandi517 – 5237Combined sources
Helixi526 – 53611Combined sources
Beta strandi543 – 5453Combined sources
Beta strandi547 – 5537Combined sources
Helixi563 – 57210Combined sources
Beta strandi576 – 5827Combined sources
Beta strandi589 – 5946Combined sources
Turni596 – 5983Combined sources
Beta strandi599 – 6068Combined sources
Turni608 – 6114Combined sources
Helixi618 – 6214Combined sources
Helixi634 – 6429Combined sources
Beta strandi645 – 6517Combined sources
Beta strandi654 – 6596Combined sources
Beta strandi669 – 6735Combined sources
Turni674 – 6763Combined sources
Beta strandi690 – 6945Combined sources
Beta strandi698 – 7069Combined sources
Beta strandi716 – 7216Combined sources
Beta strandi741 – 7444Combined sources
Beta strandi746 – 7538Combined sources
Beta strandi756 – 7616Combined sources
Turni762 – 7654Combined sources
Beta strandi766 – 7727Combined sources
Beta strandi775 – 7795Combined sources
Beta strandi785 – 7873Combined sources
Turni794 – 7996Combined sources
Helixi800 – 8067Combined sources
Helixi809 – 8113Combined sources
Beta strandi812 – 82312Combined sources
Beta strandi829 – 84012Combined sources
Beta strandi846 – 85510Combined sources
Beta strandi857 – 87216Combined sources
Beta strandi878 – 8869Combined sources
Beta strandi890 – 8989Combined sources
Beta strandi913 – 9175Combined sources
Helixi918 – 9203Combined sources
Beta strandi935 – 94511Combined sources
Turni946 – 9483Combined sources
Beta strandi949 – 95810Combined sources
Beta strandi960 – 9689Combined sources
Turni970 – 9723Combined sources
Helixi976 – 9783Combined sources
Beta strandi980 – 99314Combined sources
Helixi999 – 10013Combined sources
Helixi1007 – 10093Combined sources
Beta strandi1016 – 10249Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OB8X-ray2.80A/B/C/D2-1025[»]
3OBAX-ray2.75A/B/C/D2-1025[»]
ProteinModelPortaliP00723.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00723.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

HOGENOMiHOG000252444.
InParanoidiP00723.
KOiK01190.
OMAiRTIERDK.
OrthoDBiEOG7ZWD94.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCLIPENLR NPKKVHENRL PTRAYYYDQD IFESLNGPWA FALFDAPLDA
60 70 80 90 100
PDAKNLDWET AKKWSTISVP SHWELQEDWK YGKPIYTNVQ YPIPIDIPNP
110 120 130 140 150
PTVNPTGVYA RTFELDSKSI ESFEHRLRFE GVDNCYELYV NGQYVGFNKG
160 170 180 190 200
SRNGAEFDIQ KYVSEGENLV VVKVFKWSDS TYIEDQDQWW LSGIYRDVSL
210 220 230 240 250
LKLPKKAHIE DVRVTTTFVD SQYQDAELSV KVDVQGSSYD HINFTLYEPE
260 270 280 290 300
DGSKVYDASS LLNEENGNTT FSTKEFISFS TKKNEETAFK INVKAPEHWT
310 320 330 340 350
AENPTLYKYQ LDLIGSDGSV IQSIKHHVGF RQVELKDGNI TVNGKDILFR
360 370 380 390 400
GVNRHDHHPR FGRAVPLDFV VRDLILMKKF NINAVRNSHY PNHPKVYDLF
410 420 430 440 450
DKLGFWVIDE ADLETHGVQE PFNRHTNLEA EYPDTKNKLY DVNAHYLSDN
460 470 480 490 500
PEYEVAYLDR ASQLVLRDVN HPSIIIWSLG NEACYGRNHK AMYKLIKQLD
510 520 530 540 550
PTRLVHYEGD LNALSADIFS FMYPTFEIME RWRKNHTDEN GKFEKPLILC
560 570 580 590 600
EYGHAMGNGP GSLKEYQELF YKEKFYQGGF IWEWANHGIE FEDVSTADGK
610 620 630 640 650
LHKAYAYGGD FKEEVHDGVF IMDGLCNSEH NPTPGLVEYK KVIEPVHIKI
660 670 680 690 700
AHGSVTITNK HDFITTDHLL FIDKDTGKTI DVPSLKPEES VTIPSDTTYV
710 720 730 740 750
VAVLKDDAGV LKAGHEIAWG QAELPLKVPD FVTETAEKAA KINDGKRYVS
760 770 780 790 800
VESSGLHFIL DKLLGKIESL KVKGKEISSK FEGSSITFWR PPTNNDEPRD
810 820 830 840 850
FKNWKKYNID LMKQNIHGVS VEKGSNGSLA VVTVNSRISP VVFYYGFETV
860 870 880 890 900
QKYTIFANKI NLNTSMKLTG EYQPPDFPRV GYEFWLGDSY ESFEWLGRGP
910 920 930 940 950
GESYPDKKES QRFGLYDSKD VEEFVYDYPQ ENGNHTDTHF LNIKFEGAGK
960 970 980 990 1000
LSIFQKEKPF NFKISDEYGV DEAAHACDVK RYGRHYLRLD HAIHGVGSEA
1010 1020
CGPAVLDQYR LKAQDFNFEF DLAFE
Length:1,025
Mass (Da):117,619
Last modified:July 1, 1993 - v2
Checksum:iAF3136235DB5A464
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1.
CR382122 Genomic DNA. Translation: CAH02587.1.
X00430 Genomic DNA. Translation: CAA25128.1.
PIRiJC1266.
RefSeqiXP_452194.1. XM_452194.1.

Genome annotation databases

GeneIDi2897170.
KEGGikla:KLLA0B14883g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1 .
CR382122 Genomic DNA. Translation: CAH02587.1 .
X00430 Genomic DNA. Translation: CAA25128.1 .
PIRi JC1266.
RefSeqi XP_452194.1. XM_452194.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3OB8 X-ray 2.80 A/B/C/D 2-1025 [» ]
3OBA X-ray 2.75 A/B/C/D 2-1025 [» ]
ProteinModelPortali P00723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.P00723.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEi P00723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2897170.
KEGGi kla:KLLA0B14883g.

Phylogenomic databases

HOGENOMi HOG000252444.
InParanoidi P00723.
KOi K01190.
OMAi RTIERDK.
OrthoDBi EOG7ZWD94.

Enzyme and pathway databases

BRENDAi 3.2.1.23. 2826.
SABIO-RK P00723.

Miscellaneous databases

EvolutionaryTracei P00723.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis."
    Poch O., L'Hote H., Dallery V., Debeaux F., Fleer R., Sodoyer R.
    Gene 118:55-63(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  3. "Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product."
    Breunig K.D., Dahlems U., Das S., Hollenberg C.P.
    Nucleic Acids Res. 12:2327-2341(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-119.

Entry informationi

Entry nameiBGAL_KLULA
AccessioniPrimary (citable) accession number: P00723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1993
Last modified: November 26, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3