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P00723

- BGAL_KLULA

UniProt

P00723 - BGAL_KLULA

Protein

Beta-galactosidase

Gene

LAC4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei482 – 4821Proton donorBy similarity
    Active sitei551 – 5511NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.23. 2826.
    SABIO-RKP00723.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:LAC4
    Ordered Locus Names:KLLA0B14883g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome B

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025Beta-galactosidasePRO_0000057663Add
    BLAST

    Proteomic databases

    PRIDEiP00723.

    Interactioni

    Protein-protein interaction databases

    STRINGi28985.P00723.

    Structurei

    Secondary structure

    1
    1025
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Beta strandi16 – 183
    Turni29 – 313
    Beta strandi32 – 343
    Beta strandi37 – 4610
    Helixi47 – 493
    Helixi53 – 553
    Helixi58 – 614
    Beta strandi66 – 705
    Helixi77 – 793
    Beta strandi85 – 906
    Beta strandi106 – 11510
    Helixi117 – 1226
    Beta strandi123 – 1308
    Beta strandi132 – 1409
    Beta strandi143 – 1497
    Beta strandi155 – 1584
    Turni160 – 1623
    Beta strandi165 – 17612
    Helixi179 – 1835
    Beta strandi187 – 1904
    Beta strandi198 – 22023
    Beta strandi225 – 23713
    Beta strandi241 – 2477
    Helixi258 – 2625
    Beta strandi275 – 2806
    Beta strandi287 – 2959
    Beta strandi301 – 3044
    Beta strandi306 – 3149
    Beta strandi320 – 3289
    Beta strandi333 – 3364
    Beta strandi339 – 3424
    Beta strandi349 – 3535
    Turni359 – 3613
    Helixi367 – 37913
    Beta strandi384 – 3863
    Helixi396 – 4038
    Beta strandi406 – 4105
    Helixi416 – 4194
    Helixi420 – 4256
    Turni433 – 4364
    Helixi437 – 4404
    Turni441 – 4433
    Helixi444 – 4463
    Turni447 – 4493
    Helixi451 – 4533
    Helixi454 – 46815
    Beta strandi474 – 4785
    Helixi487 – 49913
    Beta strandi503 – 5064
    Beta strandi517 – 5237
    Helixi526 – 53611
    Beta strandi543 – 5453
    Beta strandi547 – 5537
    Helixi563 – 57210
    Beta strandi576 – 5827
    Beta strandi589 – 5946
    Turni596 – 5983
    Beta strandi599 – 6068
    Turni608 – 6114
    Helixi618 – 6214
    Helixi634 – 6429
    Beta strandi645 – 6517
    Beta strandi654 – 6596
    Beta strandi669 – 6735
    Turni674 – 6763
    Beta strandi690 – 6945
    Beta strandi698 – 7069
    Beta strandi716 – 7216
    Beta strandi741 – 7444
    Beta strandi746 – 7538
    Beta strandi756 – 7616
    Turni762 – 7654
    Beta strandi766 – 7727
    Beta strandi775 – 7795
    Beta strandi785 – 7873
    Turni794 – 7996
    Helixi800 – 8067
    Helixi809 – 8113
    Beta strandi812 – 82312
    Beta strandi829 – 84012
    Beta strandi846 – 85510
    Beta strandi857 – 87216
    Beta strandi878 – 8869
    Beta strandi890 – 8989
    Beta strandi913 – 9175
    Helixi918 – 9203
    Beta strandi935 – 94511
    Turni946 – 9483
    Beta strandi949 – 95810
    Beta strandi960 – 9689
    Turni970 – 9723
    Helixi976 – 9783
    Beta strandi980 – 99314
    Helixi999 – 10013
    Helixi1007 – 10093
    Beta strandi1016 – 10249

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OB8X-ray2.80A/B/C/D2-1025[»]
    3OBAX-ray2.75A/B/C/D2-1025[»]
    ProteinModelPortaliP00723.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00723.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000252444.
    KOiK01190.
    OMAiRTIERDK.
    OrthoDBiEOG7ZWD94.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 3 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCLIPENLR NPKKVHENRL PTRAYYYDQD IFESLNGPWA FALFDAPLDA     50
    PDAKNLDWET AKKWSTISVP SHWELQEDWK YGKPIYTNVQ YPIPIDIPNP 100
    PTVNPTGVYA RTFELDSKSI ESFEHRLRFE GVDNCYELYV NGQYVGFNKG 150
    SRNGAEFDIQ KYVSEGENLV VVKVFKWSDS TYIEDQDQWW LSGIYRDVSL 200
    LKLPKKAHIE DVRVTTTFVD SQYQDAELSV KVDVQGSSYD HINFTLYEPE 250
    DGSKVYDASS LLNEENGNTT FSTKEFISFS TKKNEETAFK INVKAPEHWT 300
    AENPTLYKYQ LDLIGSDGSV IQSIKHHVGF RQVELKDGNI TVNGKDILFR 350
    GVNRHDHHPR FGRAVPLDFV VRDLILMKKF NINAVRNSHY PNHPKVYDLF 400
    DKLGFWVIDE ADLETHGVQE PFNRHTNLEA EYPDTKNKLY DVNAHYLSDN 450
    PEYEVAYLDR ASQLVLRDVN HPSIIIWSLG NEACYGRNHK AMYKLIKQLD 500
    PTRLVHYEGD LNALSADIFS FMYPTFEIME RWRKNHTDEN GKFEKPLILC 550
    EYGHAMGNGP GSLKEYQELF YKEKFYQGGF IWEWANHGIE FEDVSTADGK 600
    LHKAYAYGGD FKEEVHDGVF IMDGLCNSEH NPTPGLVEYK KVIEPVHIKI 650
    AHGSVTITNK HDFITTDHLL FIDKDTGKTI DVPSLKPEES VTIPSDTTYV 700
    VAVLKDDAGV LKAGHEIAWG QAELPLKVPD FVTETAEKAA KINDGKRYVS 750
    VESSGLHFIL DKLLGKIESL KVKGKEISSK FEGSSITFWR PPTNNDEPRD 800
    FKNWKKYNID LMKQNIHGVS VEKGSNGSLA VVTVNSRISP VVFYYGFETV 850
    QKYTIFANKI NLNTSMKLTG EYQPPDFPRV GYEFWLGDSY ESFEWLGRGP 900
    GESYPDKKES QRFGLYDSKD VEEFVYDYPQ ENGNHTDTHF LNIKFEGAGK 950
    LSIFQKEKPF NFKISDEYGV DEAAHACDVK RYGRHYLRLD HAIHGVGSEA 1000
    CGPAVLDQYR LKAQDFNFEF DLAFE 1025
    Length:1,025
    Mass (Da):117,619
    Last modified:July 1, 1993 - v2
    Checksum:iAF3136235DB5A464
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84410 Genomic DNA. Translation: AAA35265.1.
    CR382122 Genomic DNA. Translation: CAH02587.1.
    X00430 Genomic DNA. Translation: CAA25128.1.
    PIRiJC1266.
    RefSeqiXP_452194.1. XM_452194.1.

    Genome annotation databases

    GeneIDi2897170.
    KEGGikla:KLLA0B14883g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84410 Genomic DNA. Translation: AAA35265.1 .
    CR382122 Genomic DNA. Translation: CAH02587.1 .
    X00430 Genomic DNA. Translation: CAA25128.1 .
    PIRi JC1266.
    RefSeqi XP_452194.1. XM_452194.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3OB8 X-ray 2.80 A/B/C/D 2-1025 [» ]
    3OBA X-ray 2.75 A/B/C/D 2-1025 [» ]
    ProteinModelPortali P00723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.P00723.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Proteomic databases

    PRIDEi P00723.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2897170.
    KEGGi kla:KLLA0B14883g.

    Phylogenomic databases

    HOGENOMi HOG000252444.
    KOi K01190.
    OMAi RTIERDK.
    OrthoDBi EOG7ZWD94.

    Enzyme and pathway databases

    BRENDAi 3.2.1.23. 2826.
    SABIO-RK P00723.

    Miscellaneous databases

    EvolutionaryTracei P00723.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 3 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis."
      Poch O., L'Hote H., Dallery V., Debeaux F., Fleer R., Sodoyer R.
      Gene 118:55-63(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
    3. "Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product."
      Breunig K.D., Dahlems U., Das S., Hollenberg C.P.
      Nucleic Acids Res. 12:2327-2341(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-119.

    Entry informationi

    Entry nameiBGAL_KLULA
    AccessioniPrimary (citable) accession number: P00723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3