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Protein

Beta-galactosidase

Gene

LAC4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei482Proton donorBy similarity1
Active sitei551NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 2825.
SABIO-RKP00723.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:LAC4
Ordered Locus Names:KLLA0B14883g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome B

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576631 – 1025Beta-galactosidaseAdd BLAST1025

Proteomic databases

PRIDEiP00723.

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_452194.1.

Structurei

Secondary structure

11025
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Beta strandi16 – 18Combined sources3
Turni29 – 31Combined sources3
Beta strandi32 – 34Combined sources3
Beta strandi37 – 46Combined sources10
Helixi47 – 49Combined sources3
Helixi53 – 55Combined sources3
Helixi58 – 61Combined sources4
Beta strandi66 – 70Combined sources5
Helixi77 – 79Combined sources3
Beta strandi85 – 90Combined sources6
Beta strandi106 – 115Combined sources10
Helixi117 – 122Combined sources6
Beta strandi123 – 130Combined sources8
Beta strandi132 – 140Combined sources9
Beta strandi143 – 149Combined sources7
Beta strandi155 – 158Combined sources4
Turni160 – 162Combined sources3
Beta strandi165 – 176Combined sources12
Helixi179 – 183Combined sources5
Beta strandi187 – 190Combined sources4
Beta strandi198 – 220Combined sources23
Beta strandi225 – 237Combined sources13
Beta strandi241 – 247Combined sources7
Helixi258 – 262Combined sources5
Beta strandi275 – 280Combined sources6
Beta strandi287 – 295Combined sources9
Beta strandi301 – 304Combined sources4
Beta strandi306 – 314Combined sources9
Beta strandi320 – 328Combined sources9
Beta strandi333 – 336Combined sources4
Beta strandi339 – 342Combined sources4
Beta strandi349 – 353Combined sources5
Turni359 – 361Combined sources3
Helixi367 – 379Combined sources13
Beta strandi384 – 386Combined sources3
Helixi396 – 403Combined sources8
Beta strandi406 – 410Combined sources5
Helixi416 – 419Combined sources4
Helixi420 – 425Combined sources6
Turni433 – 436Combined sources4
Helixi437 – 440Combined sources4
Turni441 – 443Combined sources3
Helixi444 – 446Combined sources3
Turni447 – 449Combined sources3
Helixi451 – 453Combined sources3
Helixi454 – 468Combined sources15
Beta strandi474 – 478Combined sources5
Helixi487 – 499Combined sources13
Beta strandi503 – 506Combined sources4
Beta strandi517 – 523Combined sources7
Helixi526 – 536Combined sources11
Beta strandi543 – 545Combined sources3
Beta strandi547 – 553Combined sources7
Helixi563 – 572Combined sources10
Beta strandi576 – 582Combined sources7
Beta strandi589 – 594Combined sources6
Turni596 – 598Combined sources3
Beta strandi599 – 606Combined sources8
Turni608 – 611Combined sources4
Helixi618 – 621Combined sources4
Helixi634 – 642Combined sources9
Beta strandi645 – 651Combined sources7
Beta strandi654 – 659Combined sources6
Beta strandi669 – 673Combined sources5
Turni674 – 676Combined sources3
Beta strandi690 – 694Combined sources5
Beta strandi698 – 706Combined sources9
Beta strandi716 – 721Combined sources6
Beta strandi741 – 744Combined sources4
Beta strandi746 – 753Combined sources8
Beta strandi756 – 761Combined sources6
Turni762 – 765Combined sources4
Beta strandi766 – 772Combined sources7
Beta strandi775 – 779Combined sources5
Beta strandi785 – 787Combined sources3
Turni794 – 799Combined sources6
Helixi800 – 806Combined sources7
Helixi809 – 811Combined sources3
Beta strandi812 – 823Combined sources12
Beta strandi829 – 840Combined sources12
Beta strandi846 – 855Combined sources10
Beta strandi857 – 872Combined sources16
Beta strandi878 – 886Combined sources9
Beta strandi890 – 898Combined sources9
Beta strandi913 – 917Combined sources5
Helixi918 – 920Combined sources3
Beta strandi935 – 945Combined sources11
Turni946 – 948Combined sources3
Beta strandi949 – 958Combined sources10
Beta strandi960 – 968Combined sources9
Turni970 – 972Combined sources3
Helixi976 – 978Combined sources3
Beta strandi980 – 993Combined sources14
Helixi999 – 1001Combined sources3
Helixi1007 – 1009Combined sources3
Beta strandi1016 – 1024Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OB8X-ray2.80A/B/C/D2-1025[»]
3OBAX-ray2.75A/B/C/D2-1025[»]
ProteinModelPortaliP00723.
SMRiP00723.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00723.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiKOG2024. Eukaryota.
COG3250. LUCA.
HOGENOMiHOG000252444.
InParanoidiP00723.
KOiK01190.
OMAiICEYAHS.
OrthoDBiEOG092C05H8.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCLIPENLR NPKKVHENRL PTRAYYYDQD IFESLNGPWA FALFDAPLDA
60 70 80 90 100
PDAKNLDWET AKKWSTISVP SHWELQEDWK YGKPIYTNVQ YPIPIDIPNP
110 120 130 140 150
PTVNPTGVYA RTFELDSKSI ESFEHRLRFE GVDNCYELYV NGQYVGFNKG
160 170 180 190 200
SRNGAEFDIQ KYVSEGENLV VVKVFKWSDS TYIEDQDQWW LSGIYRDVSL
210 220 230 240 250
LKLPKKAHIE DVRVTTTFVD SQYQDAELSV KVDVQGSSYD HINFTLYEPE
260 270 280 290 300
DGSKVYDASS LLNEENGNTT FSTKEFISFS TKKNEETAFK INVKAPEHWT
310 320 330 340 350
AENPTLYKYQ LDLIGSDGSV IQSIKHHVGF RQVELKDGNI TVNGKDILFR
360 370 380 390 400
GVNRHDHHPR FGRAVPLDFV VRDLILMKKF NINAVRNSHY PNHPKVYDLF
410 420 430 440 450
DKLGFWVIDE ADLETHGVQE PFNRHTNLEA EYPDTKNKLY DVNAHYLSDN
460 470 480 490 500
PEYEVAYLDR ASQLVLRDVN HPSIIIWSLG NEACYGRNHK AMYKLIKQLD
510 520 530 540 550
PTRLVHYEGD LNALSADIFS FMYPTFEIME RWRKNHTDEN GKFEKPLILC
560 570 580 590 600
EYGHAMGNGP GSLKEYQELF YKEKFYQGGF IWEWANHGIE FEDVSTADGK
610 620 630 640 650
LHKAYAYGGD FKEEVHDGVF IMDGLCNSEH NPTPGLVEYK KVIEPVHIKI
660 670 680 690 700
AHGSVTITNK HDFITTDHLL FIDKDTGKTI DVPSLKPEES VTIPSDTTYV
710 720 730 740 750
VAVLKDDAGV LKAGHEIAWG QAELPLKVPD FVTETAEKAA KINDGKRYVS
760 770 780 790 800
VESSGLHFIL DKLLGKIESL KVKGKEISSK FEGSSITFWR PPTNNDEPRD
810 820 830 840 850
FKNWKKYNID LMKQNIHGVS VEKGSNGSLA VVTVNSRISP VVFYYGFETV
860 870 880 890 900
QKYTIFANKI NLNTSMKLTG EYQPPDFPRV GYEFWLGDSY ESFEWLGRGP
910 920 930 940 950
GESYPDKKES QRFGLYDSKD VEEFVYDYPQ ENGNHTDTHF LNIKFEGAGK
960 970 980 990 1000
LSIFQKEKPF NFKISDEYGV DEAAHACDVK RYGRHYLRLD HAIHGVGSEA
1010 1020
CGPAVLDQYR LKAQDFNFEF DLAFE
Length:1,025
Mass (Da):117,619
Last modified:July 1, 1993 - v2
Checksum:iAF3136235DB5A464
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1.
CR382122 Genomic DNA. Translation: CAH02587.1.
X00430 Genomic DNA. Translation: CAA25128.1.
PIRiJC1266.
RefSeqiXP_452194.1. XM_452194.1.

Genome annotation databases

EnsemblFungiiCAH02587; CAH02587; KLLA0_B14883g.
GeneIDi2897170.
KEGGikla:KLLA0B14883g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1.
CR382122 Genomic DNA. Translation: CAH02587.1.
X00430 Genomic DNA. Translation: CAA25128.1.
PIRiJC1266.
RefSeqiXP_452194.1. XM_452194.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OB8X-ray2.80A/B/C/D2-1025[»]
3OBAX-ray2.75A/B/C/D2-1025[»]
ProteinModelPortaliP00723.
SMRiP00723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_452194.1.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiP00723.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH02587; CAH02587; KLLA0_B14883g.
GeneIDi2897170.
KEGGikla:KLLA0B14883g.

Phylogenomic databases

eggNOGiKOG2024. Eukaryota.
COG3250. LUCA.
HOGENOMiHOG000252444.
InParanoidiP00723.
KOiK01190.
OMAiICEYAHS.
OrthoDBiEOG092C05H8.

Enzyme and pathway databases

BRENDAi3.2.1.23. 2825.
SABIO-RKP00723.

Miscellaneous databases

EvolutionaryTraceiP00723.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_KLULA
AccessioniPrimary (citable) accession number: P00723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.