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P00723

- BGAL_KLULA

UniProt

P00723 - BGAL_KLULA

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Protein

Beta-galactosidase

Gene

LAC4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei482 – 4821Proton donorBy similarity
Active sitei551 – 5511NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 2826.
SABIO-RKP00723.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:LAC4
Ordered Locus Names:KLLA0B14883g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome B

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidasePRO_0000057663Add
BLAST

Proteomic databases

PRIDEiP00723.

Interactioni

Protein-protein interaction databases

STRINGi28985.P00723.

Structurei

Secondary structure

1
1025
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93
Beta strandi16 – 183
Turni29 – 313
Beta strandi32 – 343
Beta strandi37 – 4610
Helixi47 – 493
Helixi53 – 553
Helixi58 – 614
Beta strandi66 – 705
Helixi77 – 793
Beta strandi85 – 906
Beta strandi106 – 11510
Helixi117 – 1226
Beta strandi123 – 1308
Beta strandi132 – 1409
Beta strandi143 – 1497
Beta strandi155 – 1584
Turni160 – 1623
Beta strandi165 – 17612
Helixi179 – 1835
Beta strandi187 – 1904
Beta strandi198 – 22023
Beta strandi225 – 23713
Beta strandi241 – 2477
Helixi258 – 2625
Beta strandi275 – 2806
Beta strandi287 – 2959
Beta strandi301 – 3044
Beta strandi306 – 3149
Beta strandi320 – 3289
Beta strandi333 – 3364
Beta strandi339 – 3424
Beta strandi349 – 3535
Turni359 – 3613
Helixi367 – 37913
Beta strandi384 – 3863
Helixi396 – 4038
Beta strandi406 – 4105
Helixi416 – 4194
Helixi420 – 4256
Turni433 – 4364
Helixi437 – 4404
Turni441 – 4433
Helixi444 – 4463
Turni447 – 4493
Helixi451 – 4533
Helixi454 – 46815
Beta strandi474 – 4785
Helixi487 – 49913
Beta strandi503 – 5064
Beta strandi517 – 5237
Helixi526 – 53611
Beta strandi543 – 5453
Beta strandi547 – 5537
Helixi563 – 57210
Beta strandi576 – 5827
Beta strandi589 – 5946
Turni596 – 5983
Beta strandi599 – 6068
Turni608 – 6114
Helixi618 – 6214
Helixi634 – 6429
Beta strandi645 – 6517
Beta strandi654 – 6596
Beta strandi669 – 6735
Turni674 – 6763
Beta strandi690 – 6945
Beta strandi698 – 7069
Beta strandi716 – 7216
Beta strandi741 – 7444
Beta strandi746 – 7538
Beta strandi756 – 7616
Turni762 – 7654
Beta strandi766 – 7727
Beta strandi775 – 7795
Beta strandi785 – 7873
Turni794 – 7996
Helixi800 – 8067
Helixi809 – 8113
Beta strandi812 – 82312
Beta strandi829 – 84012
Beta strandi846 – 85510
Beta strandi857 – 87216
Beta strandi878 – 8869
Beta strandi890 – 8989
Beta strandi913 – 9175
Helixi918 – 9203
Beta strandi935 – 94511
Turni946 – 9483
Beta strandi949 – 95810
Beta strandi960 – 9689
Turni970 – 9723
Helixi976 – 9783
Beta strandi980 – 99314
Helixi999 – 10013
Helixi1007 – 10093
Beta strandi1016 – 10249

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OB8X-ray2.80A/B/C/D2-1025[»]
3OBAX-ray2.75A/B/C/D2-1025[»]
ProteinModelPortaliP00723.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00723.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

HOGENOMiHOG000252444.
InParanoidiP00723.
KOiK01190.
OMAiRTIERDK.
OrthoDBiEOG7ZWD94.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCLIPENLR NPKKVHENRL PTRAYYYDQD IFESLNGPWA FALFDAPLDA
60 70 80 90 100
PDAKNLDWET AKKWSTISVP SHWELQEDWK YGKPIYTNVQ YPIPIDIPNP
110 120 130 140 150
PTVNPTGVYA RTFELDSKSI ESFEHRLRFE GVDNCYELYV NGQYVGFNKG
160 170 180 190 200
SRNGAEFDIQ KYVSEGENLV VVKVFKWSDS TYIEDQDQWW LSGIYRDVSL
210 220 230 240 250
LKLPKKAHIE DVRVTTTFVD SQYQDAELSV KVDVQGSSYD HINFTLYEPE
260 270 280 290 300
DGSKVYDASS LLNEENGNTT FSTKEFISFS TKKNEETAFK INVKAPEHWT
310 320 330 340 350
AENPTLYKYQ LDLIGSDGSV IQSIKHHVGF RQVELKDGNI TVNGKDILFR
360 370 380 390 400
GVNRHDHHPR FGRAVPLDFV VRDLILMKKF NINAVRNSHY PNHPKVYDLF
410 420 430 440 450
DKLGFWVIDE ADLETHGVQE PFNRHTNLEA EYPDTKNKLY DVNAHYLSDN
460 470 480 490 500
PEYEVAYLDR ASQLVLRDVN HPSIIIWSLG NEACYGRNHK AMYKLIKQLD
510 520 530 540 550
PTRLVHYEGD LNALSADIFS FMYPTFEIME RWRKNHTDEN GKFEKPLILC
560 570 580 590 600
EYGHAMGNGP GSLKEYQELF YKEKFYQGGF IWEWANHGIE FEDVSTADGK
610 620 630 640 650
LHKAYAYGGD FKEEVHDGVF IMDGLCNSEH NPTPGLVEYK KVIEPVHIKI
660 670 680 690 700
AHGSVTITNK HDFITTDHLL FIDKDTGKTI DVPSLKPEES VTIPSDTTYV
710 720 730 740 750
VAVLKDDAGV LKAGHEIAWG QAELPLKVPD FVTETAEKAA KINDGKRYVS
760 770 780 790 800
VESSGLHFIL DKLLGKIESL KVKGKEISSK FEGSSITFWR PPTNNDEPRD
810 820 830 840 850
FKNWKKYNID LMKQNIHGVS VEKGSNGSLA VVTVNSRISP VVFYYGFETV
860 870 880 890 900
QKYTIFANKI NLNTSMKLTG EYQPPDFPRV GYEFWLGDSY ESFEWLGRGP
910 920 930 940 950
GESYPDKKES QRFGLYDSKD VEEFVYDYPQ ENGNHTDTHF LNIKFEGAGK
960 970 980 990 1000
LSIFQKEKPF NFKISDEYGV DEAAHACDVK RYGRHYLRLD HAIHGVGSEA
1010 1020
CGPAVLDQYR LKAQDFNFEF DLAFE
Length:1,025
Mass (Da):117,619
Last modified:July 1, 1993 - v2
Checksum:iAF3136235DB5A464
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1.
CR382122 Genomic DNA. Translation: CAH02587.1.
X00430 Genomic DNA. Translation: CAA25128.1.
PIRiJC1266.
RefSeqiXP_452194.1. XM_452194.1.

Genome annotation databases

GeneIDi2897170.
KEGGikla:KLLA0B14883g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84410 Genomic DNA. Translation: AAA35265.1 .
CR382122 Genomic DNA. Translation: CAH02587.1 .
X00430 Genomic DNA. Translation: CAA25128.1 .
PIRi JC1266.
RefSeqi XP_452194.1. XM_452194.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3OB8 X-ray 2.80 A/B/C/D 2-1025 [» ]
3OBA X-ray 2.75 A/B/C/D 2-1025 [» ]
ProteinModelPortali P00723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.P00723.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEi P00723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2897170.
KEGGi kla:KLLA0B14883g.

Phylogenomic databases

HOGENOMi HOG000252444.
InParanoidi P00723.
KOi K01190.
OMAi RTIERDK.
OrthoDBi EOG7ZWD94.

Enzyme and pathway databases

BRENDAi 3.2.1.23. 2826.
SABIO-RK P00723.

Miscellaneous databases

EvolutionaryTracei P00723.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 3 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis."
    Poch O., L'Hote H., Dallery V., Debeaux F., Fleer R., Sodoyer R.
    Gene 118:55-63(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  3. "Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product."
    Breunig K.D., Dahlems U., Das S., Hollenberg C.P.
    Nucleic Acids Res. 12:2327-2341(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-119.

Entry informationi

Entry nameiBGAL_KLULA
AccessioniPrimary (citable) accession number: P00723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3