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Protein

Alpha-lactalbumin

Gene

LALBA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi97 – 108PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • cell wall macromolecule catabolic process Source: GO_Central
  • defense response to Gram-negative bacterium Source: GO_Central
  • defense response to Gram-positive bacterium Source: GO_Central
  • lactose biosynthetic process Source: UniProtKB-KW
  • response to 11-deoxycorticosterone Source: AgBase
  • response to dehydroepiandrosterone Source: AgBase
  • response to estradiol Source: AgBase
  • response to progesterone Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Keywords - Biological processi

Lactose biosynthesis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-5653890. Lactose synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-lactalbumin
Alternative name(s):
Lactose synthase B protein
Allergen: Bos d 4
Gene namesi
Name:LALBA
Synonyms:ALACTA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Is one of the causes of cow's milk allergy.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei163. Bos d 4.
3165. Bos d 4.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000001843920 – 142Alpha-lactalbuminAdd BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 139PROSITE-ProRule annotation1 Publication
Disulfide bondi47 ↔ 130PROSITE-ProRule annotation1 Publication
Glycosylationi64N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi80 ↔ 96PROSITE-ProRule annotation1 Publication
Disulfide bondi92 ↔ 110PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP00711.
PeptideAtlasiP00711.
PRIDEiP00711.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Interactioni

Subunit structurei

Lactose synthase (LS) is a heterodimer of a catalytic component, beta1,4-galactosyltransferase (beta4Gal-T1) and a regulatory component, alpha-lactalbumin (LA).

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-7080486,EBI-7080486

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi159201. 1 interactor.
IntActiP00711. 3 interactors.
MINTiMINT-1487560.
STRINGi9913.ENSBTAP00000007701.

Structurei

Secondary structure

1142
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 30Combined sources7
Helixi32 – 34Combined sources3
Helixi37 – 39Combined sources3
Helixi42 – 53Combined sources12
Beta strandi60 – 62Combined sources3
Beta strandi67 – 69Combined sources3
Turni70 – 73Combined sources4
Turni76 – 79Combined sources4
Beta strandi80 – 82Combined sources3
Helixi96 – 100Combined sources5
Helixi105 – 117Combined sources13
Helixi120 – 122Combined sources3
Helixi124 – 129Combined sources6
Beta strandi130 – 132Combined sources3
Helixi134 – 137Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F6RX-ray2.20A/B/C/D/E/F20-142[»]
1F6SX-ray2.20A/B/C/D/E/F20-142[»]
1HFZX-ray2.30A/B/C/D20-142[»]
2G4NX-ray2.30A/B/C/D/E/F20-142[»]
ProteinModelPortaliP00711.
SMRiP00711.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00711.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IX41. Eukaryota.
ENOG410ZQK6. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiP00711.
KOiK00704.
OMAiLAHKPLC.
OrthoDBiEOG091G0R9V.
TreeFamiTF324882.

Family and domain databases

CDDicd00119. LYZ1. 1 hit.
InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000545. Lactalbumin.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00136. LACTALBUMIN.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSFVSLLLV GILFHATQAE QLTKCEVFRE LKDLKGYGGV SLPEWVCTTF
60 70 80 90 100
HTSGYDTQAI VQNNDSTEYG LFQINNKIWC KDDQNPHSSN ICNISCDKFL
110 120 130 140
DDDLTDDIMC VKKILDKVGI NYWLAHKALC SEKLDQWLCE KL
Length:142
Mass (Da):16,247
Last modified:July 1, 1989 - v2
Checksum:i810CDB1145901405
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49T → A (PubMed:3120795).Curated1
Sequence conflicti49T → A (Ref. 5) Curated1
Sequence conflicti58Q → E AA sequence (PubMed:5532231).Curated1
Sequence conflicti82 – 85DDQN → NDQD AA sequence (PubMed:5532231).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29R → Q in an allele of Droughtmaster cattle; an Australian breed. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06366 Genomic DNA. Translation: CAA29664.1.
M18780 mRNA. Translation: AAA30615.1.
J05147 mRNA. Translation: AAA30367.1.
AB052163 Genomic DNA. Translation: BAB18921.1.
AB052164 Genomic DNA. Translation: BAB18922.1.
AB052165 Genomic DNA. Translation: BAB18923.1.
AB052166 Genomic DNA. Translation: BAB18924.1.
AB052167 Genomic DNA. Translation: BAB18925.1.
AF249896 Genomic DNA. Translation: AAF63624.1.
BT025469 mRNA. Translation: ABF57425.1.
BC102173 mRNA. Translation: AAI02174.1.
M90645 Genomic DNA. Translation: AAA30614.1.
PIRiA27360. LABO.
RefSeqiNP_776803.1. NM_174378.2.
UniGeneiBt.87412.

Genome annotation databases

EnsembliENSBTAT00000007701; ENSBTAP00000007701; ENSBTAG00000005859.
GeneIDi281894.
KEGGibta:281894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06366 Genomic DNA. Translation: CAA29664.1.
M18780 mRNA. Translation: AAA30615.1.
J05147 mRNA. Translation: AAA30367.1.
AB052163 Genomic DNA. Translation: BAB18921.1.
AB052164 Genomic DNA. Translation: BAB18922.1.
AB052165 Genomic DNA. Translation: BAB18923.1.
AB052166 Genomic DNA. Translation: BAB18924.1.
AB052167 Genomic DNA. Translation: BAB18925.1.
AF249896 Genomic DNA. Translation: AAF63624.1.
BT025469 mRNA. Translation: ABF57425.1.
BC102173 mRNA. Translation: AAI02174.1.
M90645 Genomic DNA. Translation: AAA30614.1.
PIRiA27360. LABO.
RefSeqiNP_776803.1. NM_174378.2.
UniGeneiBt.87412.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F6RX-ray2.20A/B/C/D/E/F20-142[»]
1F6SX-ray2.20A/B/C/D/E/F20-142[»]
1HFZX-ray2.30A/B/C/D20-142[»]
2G4NX-ray2.30A/B/C/D/E/F20-142[»]
ProteinModelPortaliP00711.
SMRiP00711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159201. 1 interactor.
IntActiP00711. 3 interactors.
MINTiMINT-1487560.
STRINGi9913.ENSBTAP00000007701.

Protein family/group databases

Allergomei163. Bos d 4.
3165. Bos d 4.0101.

Proteomic databases

PaxDbiP00711.
PeptideAtlasiP00711.
PRIDEiP00711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007701; ENSBTAP00000007701; ENSBTAG00000005859.
GeneIDi281894.
KEGGibta:281894.

Organism-specific databases

CTDi3906.

Phylogenomic databases

eggNOGiENOG410IX41. Eukaryota.
ENOG410ZQK6. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiP00711.
KOiK00704.
OMAiLAHKPLC.
OrthoDBiEOG091G0R9V.
TreeFamiTF324882.

Enzyme and pathway databases

ReactomeiR-BTA-5653890. Lactose synthesis.

Miscellaneous databases

EvolutionaryTraceiP00711.

Family and domain databases

CDDicd00119. LYZ1. 1 hit.
InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000545. Lactalbumin.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00136. LACTALBUMIN.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLALBA_BOVIN
AccessioniPrimary (citable) accession number: P00711
Secondary accession number(s): Q3T111, Q95NE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.