Reviewed, UniProtKB/Swiss-Prot P00697 (LYSC1_RAT)

Names and origin

Protein names

Recommended name:
    Lysozyme C-1
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase C

Gene names

Name:	Lyz1
Synonyms:	Lyz

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. In the intestine they may also have a digestive function.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Tissue specificity	Expressed in lung, small intestine and spleen.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. Rat has two lysozymes, type 1 and type 2 of which only type 1 is expressed; type 2 being probably a pseudogene.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase Milk protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to Gram-negative bacterium Inferred from mutant phenotype. Source: RGD defense response to Gram-positive bacterium Inferred from mutant phenotype. Source: RGD
Cellular component	Golgi cis cisterna Inferred from direct assay. Source: RGD microvillus Inferred from direct assay. Source: RGD rough endoplasmic reticulum lumen Inferred from direct assay. Source: RGD secretory granule Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD trans-Golgi network transport vesicle Inferred from direct assay. Source: RGD
Molecular function	lysozyme activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2
	Chain	19 – 148	130	Lysozyme C-1		PRO_0000018484
Sites
	Active site	53	1	By similarity
	Active site	71	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 146		By similarity
	Disulfide bond	48 ↔ 134		By similarity
	Disulfide bond	83 ↔ 99		By similarity
	Disulfide bond	95 ↔ 113		By similarity
Experimental info
	Sequence conflict	20	1	I → T AA sequence Ref.2
	Sequence conflict	64	1	N → D AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00697-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 8E6E986539BD3EEE	FASTA	148	16,729
	10 20 30 40 50 60 MKALLVLGFL LLSASVQAKI YERCQFARTL KRNGMSGYYG VSLADWVCLA QHESNYNTQA 70 80 90 100 110 120 RNYNPGDQST DYGIFQINSR YWCNDGKTPR AKNACGIPCS ALLQDDITQA IQCAKRVVRD 130 140 PQGIRAWVAW QRHCKNRDLS GYIRNCGV

References

[1]	"Evolution of rodent lysozymes: isolation and sequence of the rat lysozyme genes." Yeh T.C., Wilson A.C., Irwin D.M. Mol. Phylogenet. Evol. 2:65-75(1993) [PubMed: 8081549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[2]	"Primary structure of rat lysozyme." White T.J., Mross G.A., Osserman E.F., Wilson A.C. Biochemistry 16:1430-1436(1977) [PubMed: 851497] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-148. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12459 Genomic DNA. Translation: AAA41551.1.
IPI	IPI00211927.
PIR	LZRT. A40729.
UniGene	Rn.2283
3D structure databases
SMR	P00697. Positions 19-148.
ModBase	Search...
Protein-protein interaction databases
STRING	P00697.
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
PTM databases
PhosphoSite	P00697.
Proteomic databases
PRIDE	P00697.
Genome annotation databases
Ensembl	ENSRNOT00000007747; ENSRNOP00000007747; ENSRNOG00000005825; Rattus norvegicus. [Genome view]
UCSC	NM_012771. rat.
Organism-specific databases
RGD	3026. Lyz.
Phylogenomic databases
eggNOG	roNOG16045.
HOVERGEN	P00697.
InParanoid	P00697.
Enzyme and pathway databases
BRENDA	3.2.1.17. 248.
Gene expression databases
ArrayExpress	P00697.
Genevestigator	P00697.
GermOnline	ENSRNOG00000005825. Rattus norvegicus.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC1_RAT

Accession

Primary (citable) accession number: P00697

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1994
Last modified:	January 19, 2010
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)