Reviewed, UniProtKB/Swiss-Prot P00694 (XYNA_BACPU)

Names and origin

Protein names

Recommended name:
    Endo-1,4-beta-xylanase A
      Short name=Xylanase A
    EC=3.2.1.8
Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A

Gene names

Name:

xynA

Organism

Bacillus pumilus (Bacillus mesentericus)

Taxonomic identifier

1408 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.

Ontologies

Keywords
Biological process	Xylan degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 228	201	Endo-1,4-beta-xylanase A		PRO_0000007997
Sites
	Active site	120	1	Nucleophile By similarity
	Active site	209	1	Proton donor By similarity
Experimental info
	Mutagenesis	120	1	E → S: Loss of activity.
	Mutagenesis	209	1	E → D: Loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	P00694-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 32EF9833E7B5E503	FASTA	228	25,491
	10 20 30 40 50 60 MNLRKLRLLF VMCIGLTLIL TAVPAHARTI TNNEMGNHSG YDYELWKDYG NTSMTLNNGG 70 80 90 100 110 120 AFSAGWNNIG NALFRKGKKF DSTRTHHQLG NISINYNASF NPGGNSYLCV YGWTQSPLAE 130 140 150 160 170 180 YYIVDSWGTY RPTGAYKGSF YADGGTYDIY ETTRVNQPSI IGIATFKQYW SVRQTKRTSG 190 200 210 220 TVSVSAHFRK WESLGMPMGK MYETAFTVEG YQSSGSANVM TNQLFIGN

References

[1]	"The complete nucleotide sequence of the xylanase gene (xynA) of Bacillus pumilus." Fukusaki E., Panbangred W., Shinmyo A., Okada H. FEBS Lett. 171:197-201(1984) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IPO.
[2]	Urabe I. Submitted (FEB-1991) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 103.
[3]	"Site-directed mutagenesis at aspartate and glutamate residues of xylanase from Bacillus pumilus." Ko E.P., Akatsuka H., Moriyama H., Shinmyo A., Hata Y., Katsube Y., Urabe I., Okada H. Biochem. J. 288:117-121(1992) [PubMed: 1359880] [Abstract] Cited for: MUTAGENESIS, ACTIVE SITES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X00660 Genomic DNA. Translation: CAA25278.1.
PIR	WWBSXP. A00848.
3D structure databases
HSSP	HSSP built from PDB template 1F5J based on UniProtKB P77853.
SMR	P00694. Positions 27-227.
ModBase	Search...
Protein family/group databases
CAZy	GH11. Glycoside Hydrolase Family 11.
Enzyme and pathway databases
BRENDA	3.2.1.8. 1189.
Family and domain databases
InterPro	IPR001137. Glyco_hydro_11. IPR013319. Glyco_hydro_11/12_cat. IPR018208. Glyco_hydro_11_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.
Pfam	PF00457. Glyco_hydro_11. 1 hit. [Graphical view]
PRINTS	PR00911. GLHYDRLASE11.
PROSITE	PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit. PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_BACPU

Accession

Primary (citable) accession number: P00694

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)