Reviewed, UniProtKB/Swiss-Prot P00689 (AMYP_RAT)

Names and origin

Protein names

Recommended name:
    Pancreatic alpha-amylase
      Short name=PA
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:

Amy2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 chloride ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Chloride Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Chain	16 – 508	493	Pancreatic alpha-amylase		PRO_0000001400
Sites
	Active site	209	1	Nucleophile By similarity
	Active site	245	1	Proton donor By similarity
	Active site	312	1	By similarity
	Metal binding	115	1	Calcium By similarity
	Metal binding	170	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	179	1	Calcium By similarity
	Metal binding	213	1	Calcium; via carbonyl oxygen By similarity
	Binding site	207	1	Chloride By similarity
	Binding site	310	1	Chloride By similarity
	Binding site	349	1	Chloride By similarity
Amino acid modifications
	Modified residue	16	1	Pyrrolidone carboxylic acid By similarity
	Disulfide bond	43 ↔ 101		By similarity
	Disulfide bond	85 ↔ 130		By similarity
	Disulfide bond	156 ↔ 172		By similarity
	Disulfide bond	390 ↔ 396		By similarity
	Disulfide bond	462 ↔ 474		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00689-1 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 8AA0EA5D45A7CBFC	FASTA	508	57,177
	10 20 30 40 50 60 MKFVLLLSLI GFCWAQYDPH TADGRTAIVH LFEWRWADIA KECERYLAPK GFGGVQVSPP 70 80 90 100 110 120 NENIIINNPS RPWWERYQPI SYKICSRSGN ENEFKDMVTR CNNVGVRIYV DAVINHMCGS 130 140 150 160 170 180 GNSAGTHSTC GSYFNPNNRE FSAVPYSAWY FNDNKCNGEI NNYNDANQVR NCRLSGLLDL 190 200 210 220 230 240 ALDKDYVRTK VADYMNNLID IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP 250 260 270 280 290 300 FIFQEVIDLG GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGFVP 310 320 330 340 350 360 TDRALVFVDN HDNQRGHGAG GASILTFWDA RMYKMAVGFM LAHPYGFTRV MSSYRRTRNF 370 380 390 400 410 420 QNGKDVNDWI GPPNNNGVTK EVTINPDTTC GNDWVCEHRW RQIRNMVAFR NVVNGQPFAN 430 440 450 460 470 480 WWDNGSNQVA FSRGNRGFIV FNNDDWALSS TLQTGLPAGT YCDVISGDKV NGNCTGLKVN 490 500 VGSDGKAHFS ISNSAEDPFI AIHADSKL

References

[1]	MacDonald R. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of a family of rat amylase genes." McDonald R.J., Crerar M.M., Swain W.F., Pictet R.L., Thomas G., Rutter W.J. Nature 287:117-122(1980) [PubMed: 6159531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-508.
[3]	"Pancreas-specific genes: structure and expression." McDonald R.J., Crerar M.M., Swain W.F., Pictet R.L., Rutter W.J. Cancer 47:1497-1504(1981) [PubMed: 6168351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-508.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J00703 mRNA. Translation: AAA40725.2.
IPI	IPI00211904.
PIR	ALRTP. A00840.
RefSeq	NP_113690.1.
UniGene	Rn.67070
3D structure databases
HSSP	HSSP built from PDB template 1KXQ based on UniProtKB P00690.
SMR	P00689. Positions 16-508.
ModBase	Search...
Protein-protein interaction databases
STRING	P00689.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Proteomic databases
PRIDE	P00689.
Genome annotation databases
Ensembl	ENSRNOT00000022268; ENSRNOP00000022268; ENSRNOG00000030590; Rattus norvegicus. [Genome view]
GeneID	497039.
KEGG	rno:497039.
Organism-specific databases
CTD	497039.
RGD	1593187. Amy2.
Phylogenomic databases
HOVERGEN	P00689.
Enzyme and pathway databases
BRENDA	3.2.1.1. 248.
Gene expression databases
Genevestigator	P00689.
GermOnline	ENSRNOG00000030590. Rattus norvegicus.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	697675.

Entry information

Entry name

AMYP_RAT

Accession

Primary (citable) accession number: P00689

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 20, 2002
Last modified:	October 13, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)