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Protein

Pancreatic alpha-amylase

Gene

Amy2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151CalciumBy similarity
Metal bindingi170 – 1701Calcium; via carbonyl oxygenBy similarity
Metal bindingi179 – 1791CalciumBy similarity
Binding sitei207 – 2071ChlorideBy similarity
Active sitei209 – 2091NucleophileBy similarity
Metal bindingi213 – 2131Calcium; via carbonyl oxygenBy similarity
Active sitei245 – 2451Proton donorBy similarity
Binding sitei310 – 3101ChlorideBy similarity
Sitei312 – 3121Transition state stabilizerBy similarity
Binding sitei349 – 3491ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic alpha-amylase (EC:3.2.1.1By similarity)
Short name:
PA
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1593187. Amy2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2699.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Chaini16 – 508493Pancreatic alpha-amylasePRO_0000001400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161Pyrrolidone carboxylic acidBy similarity
Disulfide bondi43 ↔ 101By similarity
Disulfide bondi85 ↔ 130By similarity
Disulfide bondi156 ↔ 172By similarity
Disulfide bondi390 ↔ 396By similarity
Disulfide bondi462 ↔ 474By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP00689.
PRIDEiP00689.

PTM databases

iPTMnetiP00689.
PhosphoSiteiP00689.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP00689. 1 interaction.
STRINGi10116.ENSRNOP00000022268.

Chemistry

BindingDBiP00689.

Structurei

3D structure databases

ProteinModelPortaliP00689.
SMRiP00689. Positions 16-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00689.
KOiK01176.
PhylomeDBiP00689.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVLLLSLI GFCWAQYDPH TADGRTAIVH LFEWRWADIA KECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENIIINNPS RPWWERYQPI SYKICSRSGN ENEFKDMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGS GNSAGTHSTC GSYFNPNNRE FSAVPYSAWY
160 170 180 190 200
FNDNKCNGEI NNYNDANQVR NCRLSGLLDL ALDKDYVRTK VADYMNNLID
210 220 230 240 250
IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP FIFQEVIDLG
260 270 280 290 300
GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGFVP
310 320 330 340 350
TDRALVFVDN HDNQRGHGAG GASILTFWDA RMYKMAVGFM LAHPYGFTRV
360 370 380 390 400
MSSYRRTRNF QNGKDVNDWI GPPNNNGVTK EVTINPDTTC GNDWVCEHRW
410 420 430 440 450
RQIRNMVAFR NVVNGQPFAN WWDNGSNQVA FSRGNRGFIV FNNDDWALSS
460 470 480 490 500
TLQTGLPAGT YCDVISGDKV NGNCTGLKVN VGSDGKAHFS ISNSAEDPFI

AIHADSKL
Length:508
Mass (Da):57,177
Last modified:June 20, 2002 - v2
Checksum:i8AA0EA5D45A7CBFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00703 mRNA. Translation: AAA40725.2.
PIRiA00840. ALRTP.
RefSeqiNP_113690.1. NM_031502.1.
UniGeneiRn.67070.

Genome annotation databases

GeneIDi497039.
KEGGirno:497039.
UCSCiRGD:1593187. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00703 mRNA. Translation: AAA40725.2.
PIRiA00840. ALRTP.
RefSeqiNP_113690.1. NM_031502.1.
UniGeneiRn.67070.

3D structure databases

ProteinModelPortaliP00689.
SMRiP00689. Positions 16-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00689. 1 interaction.
STRINGi10116.ENSRNOP00000022268.

Chemistry

BindingDBiP00689.
ChEMBLiCHEMBL2699.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiP00689.
PhosphoSiteiP00689.

Proteomic databases

PaxDbiP00689.
PRIDEiP00689.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi497039.
KEGGirno:497039.
UCSCiRGD:1593187. rat.

Organism-specific databases

CTDi100043686.
RGDi1593187. Amy2.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00689.
KOiK01176.
PhylomeDBiP00689.

Miscellaneous databases

PROiP00689.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYP_RAT
AccessioniPrimary (citable) accession number: P00689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 20, 2002
Last modified: June 8, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.