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Protein

Pancreatic alpha-amylase

Gene

Amy2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumBy similarity1
Metal bindingi170Calcium; via carbonyl oxygenBy similarity1
Metal bindingi179CalciumBy similarity1
Binding sitei207ChlorideBy similarity1
Active sitei209NucleophileBy similarity1
Metal bindingi213Calcium; via carbonyl oxygenBy similarity1
Active sitei245Proton donorBy similarity1
Binding sitei310ChlorideBy similarity1
Sitei312Transition state stabilizerBy similarity1
Binding sitei349ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: UniProtKB
  • amylase activity Source: MGI
  • calcium ion binding Source: UniProtKB
  • chloride ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-189085. Digestion of dietary carbohydrate.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic alpha-amylase (EC:3.2.1.1By similarity)
Short name:
PA
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy2
Synonyms:Amy2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88020. Amy2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000139816 – 508Pancreatic alpha-amylaseAdd BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi43 ↔ 101By similarity
Disulfide bondi85 ↔ 130By similarity
Disulfide bondi156 ↔ 172By similarity
Disulfide bondi390 ↔ 396By similarity
Disulfide bondi462 ↔ 474By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP00688.
PaxDbiP00688.
PeptideAtlasiP00688.
PRIDEiP00688.

2D gel databases

SWISS-2DPAGEP00688.

PTM databases

iPTMnetiP00688.
PhosphoSitePlusiP00688.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074268.
GenevisibleiP00688. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137025.

Structurei

3D structure databases

ProteinModelPortaliP00688.
SMRiP00688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00688.
KOiK01176.
OMAiDANDWIG.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVLLLSLI GFCWAQYDPH TSDGRTAIVH LFEWRWVDIA KECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVVVHNPS RPWWERYQPI SYKICTRSGN EDEFRDMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGA GNPAGTSSTC GSYLNPNNRE FPAVPYSAWD
160 170 180 190 200
FNDNKCNGEI DNYNDAYQVR NCRLTGLLDL ALEKDYVRTK VADYMNHLID
210 220 230 240 250
IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP FIFQEVIDLG
260 270 280 290 300
GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGLVP
310 320 330 340 350
SDRALVFVDN HDNQRGHGAG GSSILTFWDA RMYKMAVGFM LAHPYGFTRV
360 370 380 390 400
MSSYRWNRNF QNGKDQNDWI GPPNNNGVTK EVTINADTTC GNDWVCEHRW
410 420 430 440 450
RQIRNMVAFR NVVNGQPFSN WWDNNSNQVA FSRGNRGFIV FNNDDWALSA
460 470 480 490 500
TLQTGLPAGT YCDVISGDKV DGNCTGLRVN VGSDGKAHFS ISNSAEDPFI

AIHADSKL
Length:508
Mass (Da):57,318
Last modified:July 27, 2011 - v2
Checksum:iB204B03768132593
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70S → T in AAA37228 (PubMed:6176715).Curated1
Sequence conflicti85C → S in AAA37228 (PubMed:6176715).Curated1
Sequence conflicti217G → R in CAA24098 (PubMed:6157477).Curated1
Sequence conflicti217G → R in CAA26413 (PubMed:6098446).Curated1
Sequence conflicti217G → R in CAA26414 (PubMed:6098446).Curated1
Sequence conflicti217G → R in CAA26415 (PubMed:6098446).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti64V → I in A1, B(C) and AMY-2.2Y. 1
Natural varianti66V → I in AMY-2.2Y. 1
Natural varianti120A → S in A1, B(A) and B(C). 1
Natural varianti161D → S in A1, B(A) and B(C); requires 2 nucleotide substitutions. 1
Natural varianti174L → V in A1 and B(C). 1
Natural varianti175T → S in B(A). 1
Natural varianti254I → V in A1 and B(C). 1
Natural varianti270 – 272YGA → FGV in A1 and B(C). 3
Natural varianti298L → M in A1, B(A) and B(C). Combined sources1
Natural varianti322S → A in B(A). 1
Natural varianti419S → A in B1. 1
Natural varianti450A → E in B(C). 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00718 mRNA. Translation: CAA24098.1.
X02576 mRNA. Translation: CAA26413.1.
X02577 mRNA. Translation: CAA26414.1.
X02578 mRNA. Translation: CAA26415.1.
AK133526 mRNA. Translation: BAE21706.1.
BC094924 mRNA. Translation: AAH94924.1.
BC100579 mRNA. Translation: AAI00580.1.
J00357 Genomic DNA. Translation: AAA37228.1.
J00358 Genomic DNA. Translation: AAA37229.1.
J00361 Genomic DNA. Translation: AAA37233.1.
X02343 Genomic DNA. Translation: CAA26202.1.
M16540 Genomic DNA. Translation: AAA37223.1.
M15965 Genomic DNA. Translation: AAA37226.1.
M11895 mRNA. Translation: AAA37224.1.
M11896 mRNA. Translation: AAA37227.1.
M11891 Genomic DNA. Translation: AAA37222.1.
CCDSiCCDS17775.1.
PIRiA90995. ALMSP1.
B90798. ALMSP.
B90995. ALMSPC.
C90995. ALMSPA.
I49493.
I49494.
RefSeqiNP_001036176.1. NM_001042711.2.
NP_001153622.1. NM_001160150.1.
NP_001153623.1. NM_001160151.1.
NP_001153624.1. NM_001160152.1.
UniGeneiMm.275426.
Mm.443376.
Mm.462977.

Genome annotation databases

EnsembliENSMUST00000098667; ENSMUSP00000096264; ENSMUSG00000096569.
ENSMUST00000098673; ENSMUSP00000096270; ENSMUSG00000074268.
ENSMUST00000179314; ENSMUSP00000136894; ENSMUSG00000093931.
ENSMUST00000179568; ENSMUSP00000137025; ENSMUSG00000096770.
GeneIDi100043684.
100043686.
100043688.
109959.
KEGGimmu:100043684.
mmu:100043686.
mmu:100043688.
mmu:109959.
UCSCiuc008rav.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00718 mRNA. Translation: CAA24098.1.
X02576 mRNA. Translation: CAA26413.1.
X02577 mRNA. Translation: CAA26414.1.
X02578 mRNA. Translation: CAA26415.1.
AK133526 mRNA. Translation: BAE21706.1.
BC094924 mRNA. Translation: AAH94924.1.
BC100579 mRNA. Translation: AAI00580.1.
J00357 Genomic DNA. Translation: AAA37228.1.
J00358 Genomic DNA. Translation: AAA37229.1.
J00361 Genomic DNA. Translation: AAA37233.1.
X02343 Genomic DNA. Translation: CAA26202.1.
M16540 Genomic DNA. Translation: AAA37223.1.
M15965 Genomic DNA. Translation: AAA37226.1.
M11895 mRNA. Translation: AAA37224.1.
M11896 mRNA. Translation: AAA37227.1.
M11891 Genomic DNA. Translation: AAA37222.1.
CCDSiCCDS17775.1.
PIRiA90995. ALMSP1.
B90798. ALMSP.
B90995. ALMSPC.
C90995. ALMSPA.
I49493.
I49494.
RefSeqiNP_001036176.1. NM_001042711.2.
NP_001153622.1. NM_001160150.1.
NP_001153623.1. NM_001160151.1.
NP_001153624.1. NM_001160152.1.
UniGeneiMm.275426.
Mm.443376.
Mm.462977.

3D structure databases

ProteinModelPortaliP00688.
SMRiP00688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137025.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiP00688.
PhosphoSitePlusiP00688.

2D gel databases

SWISS-2DPAGEP00688.

Proteomic databases

MaxQBiP00688.
PaxDbiP00688.
PeptideAtlasiP00688.
PRIDEiP00688.

Protocols and materials databases

DNASUi109959.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098667; ENSMUSP00000096264; ENSMUSG00000096569.
ENSMUST00000098673; ENSMUSP00000096270; ENSMUSG00000074268.
ENSMUST00000179314; ENSMUSP00000136894; ENSMUSG00000093931.
ENSMUST00000179568; ENSMUSP00000137025; ENSMUSG00000096770.
GeneIDi100043684.
100043686.
100043688.
109959.
KEGGimmu:100043684.
mmu:100043686.
mmu:100043688.
mmu:109959.
UCSCiuc008rav.2. mouse.

Organism-specific databases

CTDi100043684.
100043686.
100043688.
109959.
MGIiMGI:88020. Amy2.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00688.
KOiK01176.
OMAiDANDWIG.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Enzyme and pathway databases

ReactomeiR-MMU-189085. Digestion of dietary carbohydrate.

Miscellaneous databases

PROiP00688.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074268.
GenevisibleiP00688. MM.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYP_MOUSE
AccessioniPrimary (citable) accession number: P00688
Secondary accession number(s): Q4VBW6
, Q61295, Q61296, Q64301
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.