Pancreatic alpha-amylase precursor - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Pancreatic alpha-amylase
Short name=PA
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	Amy2
Synonyms:	Amy2a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 chloride ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Calcium Chloride Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	alpha-amylase activity Inferred from sequence or structural similarity. Source: UniProtKB calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB chloride ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

15

Chain

16 – 508

493

Pancreatic alpha-amylase

PRO_0000001398

Sites

Active site

209

1

Nucleophile By similarity

Active site

245

1

Proton donor By similarity

Metal binding

115

1

Calcium By similarity

Metal binding

170

1

Calcium; via carbonyl oxygen By similarity

Metal binding

179

1

Calcium By similarity

Metal binding

213

1

Calcium; via carbonyl oxygen By similarity

Binding site

207

1

Chloride By similarity

Binding site

310

1

Chloride By similarity

Binding site

349

1

Chloride By similarity

Site

312

1

Transition state stabilizer By similarity

Amino acid modifications

Modified residue

16

1

Pyrrolidone carboxylic acid

Disulfide bond

43 ↔ 101

By similarity

Disulfide bond

85 ↔ 130

By similarity

Disulfide bond

156 ↔ 172

By similarity

Disulfide bond

390 ↔ 396

By similarity

Disulfide bond

462 ↔ 474

By similarity

Natural variations

Natural variant

64

1

V → I in A1, B(C) and AMY-2.2Y.

Natural variant

66

1

V → I in AMY-2.2Y.

Natural variant

120

1

A → S in A1, B(A) and B(C).

Natural variant

161

1

D → S in A1, B(A) and B(C); requires 2 nucleotide substitutions.

Natural variant

174

1

L → V in A1 and B(C).

Natural variant

175

1

T → S in B(A).

Natural variant

254

1

I → V in A1 and B(C).

Natural variant

270 – 272

3

YGA → FGV in A1 and B(C).

Natural variant

298

1

L → M in A1, B(A) and B(C).

Natural variant

322

1

S → A in B(A).

Natural variant

419

1

S → A in B1.

Natural variant

450

1

A → E in B(C).

Experimental info

Sequence conflict

70

1

S → T in AAA37228. Ref.5

Sequence conflict

85

1

C → S in AAA37228. Ref.5

Sequence conflict

217

1

G → R in CAA24098. Ref.1

Sequence conflict

217

1

G → R in CAA26413. Ref.2

Sequence conflict

217

1

G → R in CAA26414. Ref.2

Sequence conflict

217

1

G → R in CAA26415. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P00688 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: B204B03768132593
Show »

FASTA

508

57,318

        10         20         30         40         50         60 
MKFVLLLSLI GFCWAQYDPH TSDGRTAIVH LFEWRWVDIA KECERYLAPK GFGGVQVSPP 

        70         80         90        100        110        120 
NENVVVHNPS RPWWERYQPI SYKICTRSGN EDEFRDMVTR CNNVGVRIYV DAVINHMCGA 

       130        140        150        160        170        180 
GNPAGTSSTC GSYLNPNNRE FPAVPYSAWD FNDNKCNGEI DNYNDAYQVR NCRLTGLLDL 

       190        200        210        220        230        240 
ALEKDYVRTK VADYMNHLID IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP 

       250        260        270        280        290        300 
FIFQEVIDLG GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGLVP 

       310        320        330        340        350        360 
SDRALVFVDN HDNQRGHGAG GSSILTFWDA RMYKMAVGFM LAHPYGFTRV MSSYRWNRNF 

       370        380        390        400        410        420 
QNGKDQNDWI GPPNNNGVTK EVTINADTTC GNDWVCEHRW RQIRNMVAFR NVVNGQPFSN 

       430        440        450        460        470        480 
WWDNNSNQVA FSRGNRGFIV FNNDDWALSA TLQTGLPAGT YCDVISGDKV DGNCTGLRVN 

       490        500 
VGSDGKAHFS ISNSAEDPFI AIHADSKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland." Hagenbuechle O., Bovey R., Young R.A. Cell 21:179-187(1980) [PubMed: 6157477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Multiple non-allelic genes encoding pancreatic alpha-amylase of mouse are expressed in a strain-specific fashion." Tosi M., Bovey R., Astolfi S., Bodary S., Meisler M.H., Wellauer P.K. EMBO J. 3:2809-2816(1984) [PubMed: 6098446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOZYMES A1; B(A) AND B(C)). Strain: CE/J.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Ovary and Uterus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[5]	"The mouse alpha-amylase multigene family. Sequence organization of members expressed in the pancreas, salivary gland and liver." Schibler U., Pittet A.-C., Young R.A., Hagenbuechle O., Tosi M., Gellman S., Wellauer P.K. J. Mol. Biol. 155:247-266(1982) [PubMed: 6176715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94 AND 267-290. Strain: A/J.
[6]	"Members of the Amy-2 alpha-amylase gene family of mouse strain CE/J contain duplicated 5' termini." Bodary S., Grossi G., Hagenbuechle O., Wellauer P.K. J. Mol. Biol. 182:1-10(1985) [PubMed: 2987507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94.
[7]	"Tissue-specific and insulin-dependent expression of a pancreatic amylase gene in transgenic mice." Osborn L., Rosenberg M.P., Keller S.A., Meisler M.H. Mol. Cell. Biol. 7:326-334(1987) [PubMed: 2436036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94 (ISOZYMES AMY-2.1Y AND AMY-2.2Y).
[8]	"Evolution of the amylase multigene family. YBR/Ki mice express a pancreatic amylase gene which is silent in other strains." Gumucio D.L., Wiebauer K., Dranginis A., Samuelson L.C., Treisman L.O., Caldwell R.M., Antonucci T.K., Meisler M.H. J. Biol. Chem. 260:13483-13489(1985) [PubMed: 2414282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 267-289 AND 388-470 (ISOZYMES A1 AND B1). Strain: YRB/KI.
[9]	"Characterization of the amino termini of mouse salivary and pancreatic amylases." Karn R.C., Petersen T.E., Hjorth J.P., Nieles J.T., Roepstorff P. FEBS Lett. 126:293-296(1981) [PubMed: 6165618] [Abstract] Cited for: SIGNAL SEQUENCE CLEAVAGE SITE.
[10]	"Two distinct pancreatic amylase genes are active in YBR mice." Strahler J.R., Meisler M. Genetics 101:91-102(1982) [PubMed: 6180955] [Abstract] Cited for: PROTEIN SEQUENCE OF 407-458. Strain: YBR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00718 mRNA. Translation: CAA24098.1. X02576 mRNA. Translation: CAA26413.1. X02577 mRNA. Translation: CAA26414.1. X02578 mRNA. Translation: CAA26415.1. AK133526 mRNA. Translation: BAE21706.1. BC094924 mRNA. Translation: AAH94924.1. BC100579 mRNA. Translation: AAI00580.1. J00357 Genomic DNA. Translation: AAA37228.1. J00358 Genomic DNA. Translation: AAA37229.1. J00361 Genomic DNA. Translation: AAA37233.1. X02343 Genomic DNA. Translation: CAA26202.1. M16540 Genomic DNA. Translation: AAA37223.1. M15965 Genomic DNA. Translation: AAA37226.1. M11895 mRNA. Translation: AAA37224.1. M11896 mRNA. Translation: AAA37227.1. M11891 Genomic DNA. Translation: AAA37222.1.
IPI	IPI00133544.
PIR	ALMSP1. A90995. ALMSP. B90798. ALMSPC. B90995. ALMSPA. C90995. I49493. I49494.
RefSeq	NP_001036176.1. NM_001042711.2. NP_001153622.1. NM_001160150.1. NP_001153623.1. NM_001160151.1. NP_001153624.1. NM_001160152.1.
UniGene	Mm.275426. Mm.443376. Mm.482445. Mm.482540.
3D structure databases
ProteinModelPortal	P00688.
SMR	P00688. Positions 16-508.
ModBase	Search...
Protein-protein interaction databases
STRING	P00688.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
PTM databases
PhosphoSite	P00688.
2D gel databases
SWISS-2DPAGE	P00688.
Proteomic databases
PRIDE	P00688.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000098667; ENSMUSP00000096264; ENSMUSG00000079850. ENSMUST00000098670; ENSMUSP00000096267; ENSMUSG00000074266. ENSMUST00000098671; ENSMUSP00000096268; ENSMUSG00000074267. ENSMUST00000098673; ENSMUSP00000096270; ENSMUSG00000074268. ENSMUST00000170843; ENSMUSP00000128641; ENSMUSG00000079850.
GeneID	100043684. 100043686. 100043688. 109959.
KEGG	mmu:100043684. mmu:100043686. mmu:100043688. mmu:109959.
Organism-specific databases
CTD	100043684. 100043686. 100043688. 109959.
MGI	MGI:88020. Amy2.
Phylogenomic databases
eggNOG	roNOG12919.
HOGENOM	HBG404170.
HOVERGEN	HBG000061.
InParanoid	P00688.
OrthoDB	EOG4RV2R1.
Gene expression databases
Genevestigator	P00688.
GermOnline	ENSMUSG00000074265. Mus musculus. ENSMUSG00000074266. Mus musculus. ENSMUSG00000074267. Mus musculus. ENSMUSG00000074268. Mus musculus.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01176.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	279405.
SOURCE	Search...

Entry information

Entry name

AMYP_MOUSE

Accession

Primary (citable) accession number: P00688
Secondary accession number(s): Q4VBW6

Q64301

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents