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Protein

Alpha-amylase 1

Gene

Amy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumBy similarity1
Metal bindingi173Calcium; via carbonyl oxygenBy similarity1
Metal bindingi182CalciumBy similarity1
Binding sitei210ChlorideBy similarity1
Active sitei212NucleophileBy similarity1
Metal bindingi216Calcium; via carbonyl oxygenBy similarity1
Active sitei248Proton donorBy similarity1
Binding sitei313ChlorideBy similarity1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: MGI
  • amylase activity Source: MGI
  • calcium ion binding Source: MGI
  • chloride ion binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-189085. Digestion of dietary carbohydrate.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase 1
Salivary and hepatic alpha-amylase
Gene namesi
Name:Amy1
Synonyms:Amy-1-a, Amy1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88019. Amy1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000140316 – 511Alpha-amylase 1Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi43 ↔ 101By similarity
Disulfide bondi85 ↔ 130By similarity
Disulfide bondi156 ↔ 175By similarity
Disulfide bondi393 ↔ 399By similarity
Disulfide bondi465 ↔ 477By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP00687.
PaxDbiP00687.
PeptideAtlasiP00687.
PRIDEiP00687.

PTM databases

iPTMnetiP00687.
PhosphoSitePlusiP00687.

Expressioni

Tissue specificityi

Expressed in liver and saliva.

Gene expression databases

BgeeiENSMUSG00000074264.
CleanExiMM_AMY1.
ExpressionAtlasiP00687. baseline and differential.
GenevisibleiP00687. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi198093. 1 interactor.
IntActiP00687. 16 interactors.
STRINGi10090.ENSMUSP00000070368.

Structurei

3D structure databases

ProteinModelPortaliP00687.
SMRiP00687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00687.
KOiK01176.
OMAiWCIVAGQ.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFLLLSLI GFCWAQYDPH TQYGRTAIVH LFEWRWVDIA KECERYLAPN
60 70 80 90 100
GFAGVQVSPP NENIVVHSPS RPWWERYQPI SYKICSRSGN EDEFRDMVNR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGV GAQAGQSSTC GSYFNPNNRD FPGVPYSGFD
160 170 180 190 200
FNDGKCRTAS GGIENYQDAA QVRDCRLSGL LDLALEKDYV RTKVADYMNH
210 220 230 240 250
LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNTKWFSQG SRPFIFQEVI
260 270 280 290 300
DLGGEAVSSN EYFGNGRVTE FKYGAKLGKV MRKWDGEKMS YLKNWGEGWG
310 320 330 340 350
LMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYYWP RNFQNGKDVN DWVGPPNNNG KTKEVSINPD STCGNDWICE
410 420 430 440 450
HRWRQIRNMV AFRNVVNGQP FANWWDNDSN QVAFGRGNKG FIVFNNDDWA
460 470 480 490 500
LSETLQTGLP AGTYCDVISG DKVDGNCTGI KVYVGNDGKA HFSISNSAED
510
PFIAIHAESK I
Length:511
Mass (Da):57,644
Last modified:July 27, 2011 - v2
Checksum:iCC5399F4C23E1105
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29V → I in AAA37221 (PubMed:6157477).Curated1
Sequence conflicti29V → I in CAA24097 (PubMed:6157477).Curated1
Sequence conflicti29V → I in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti29V → I in CAA24100 (PubMed:6162108).Curated1
Sequence conflicti29V → I in AAA37219 (PubMed:6162570).Curated1
Sequence conflicti229L → Q in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti229L → Q in CAA24100 (PubMed:6162108).Curated1
Sequence conflicti441F → L in AAA37221 (PubMed:6157477).Curated1
Sequence conflicti441F → L in CAA24097 (PubMed:6157477).Curated1
Sequence conflicti441F → L in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti441F → L in CAA24100 (PubMed:6162108).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00356 mRNA. Translation: AAA37221.1.
V00717 mRNA. Translation: CAA24097.1.
V00719 mRNA. Translation: CAA24099.1.
V00720 mRNA. Translation: CAA24100.1.
AK029642 mRNA. Translation: BAC26543.1.
CH466532 Genomic DNA. Translation: EDL12410.1.
CH466532 Genomic DNA. Translation: EDL12411.1.
CH466532 Genomic DNA. Translation: EDL12412.1.
BC009121 mRNA. Translation: AAH09121.1.
J00353 Genomic DNA. Translation: AAA37219.1.
J00354 Genomic DNA. Translation: AAA37220.1.
CCDSiCCDS17776.1.
PIRiA90798. ALMSS.
RefSeqiNP_001103975.1. NM_001110505.1.
NP_031472.2. NM_007446.2.
UniGeneiMm.439727.

Genome annotation databases

EnsembliENSMUST00000067980; ENSMUSP00000070368; ENSMUSG00000074264.
ENSMUST00000106540; ENSMUSP00000102150; ENSMUSG00000074264.
GeneIDi11722.
KEGGimmu:11722.
UCSCiuc008rba.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00356 mRNA. Translation: AAA37221.1.
V00717 mRNA. Translation: CAA24097.1.
V00719 mRNA. Translation: CAA24099.1.
V00720 mRNA. Translation: CAA24100.1.
AK029642 mRNA. Translation: BAC26543.1.
CH466532 Genomic DNA. Translation: EDL12410.1.
CH466532 Genomic DNA. Translation: EDL12411.1.
CH466532 Genomic DNA. Translation: EDL12412.1.
BC009121 mRNA. Translation: AAH09121.1.
J00353 Genomic DNA. Translation: AAA37219.1.
J00354 Genomic DNA. Translation: AAA37220.1.
CCDSiCCDS17776.1.
PIRiA90798. ALMSS.
RefSeqiNP_001103975.1. NM_001110505.1.
NP_031472.2. NM_007446.2.
UniGeneiMm.439727.

3D structure databases

ProteinModelPortaliP00687.
SMRiP00687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198093. 1 interactor.
IntActiP00687. 16 interactors.
STRINGi10090.ENSMUSP00000070368.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiP00687.
PhosphoSitePlusiP00687.

Proteomic databases

MaxQBiP00687.
PaxDbiP00687.
PeptideAtlasiP00687.
PRIDEiP00687.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067980; ENSMUSP00000070368; ENSMUSG00000074264.
ENSMUST00000106540; ENSMUSP00000102150; ENSMUSG00000074264.
GeneIDi11722.
KEGGimmu:11722.
UCSCiuc008rba.2. mouse.

Organism-specific databases

CTDi11722.
MGIiMGI:88019. Amy1.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP00687.
KOiK01176.
OMAiWCIVAGQ.
OrthoDBiEOG091G07V6.
TreeFamiTF312850.

Enzyme and pathway databases

ReactomeiR-MMU-189085. Digestion of dietary carbohydrate.

Miscellaneous databases

PROiP00687.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074264.
CleanExiMM_AMY1.
ExpressionAtlasiP00687. baseline and differential.
GenevisibleiP00687. MM.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_MOUSE
AccessioniPrimary (citable) accession number: P00687
Secondary accession number(s): Q921Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Hepatic and salivary alpha-amylases are encoded by the same gene; however, their mRNAs have different 5'-UTR sequences.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.