P00686 (RNAS1_MACRU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Macropus rufus (Red kangaroo) (Megaleia rufa)

Taxonomic identifier

9321 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Diprotodontia › Macropodidae › Macropus

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Post-translational modification	Not glycosylated although the sequence N-V-T, a recognition site for carbohydrate attachment, is present.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	Ribonuclease pancreatic		PRO_0000057204
Regions
	Region	40 – 44	5	Substrate binding By similarity
Sites
	Active site	11	1	Proton acceptor By similarity
	Active site	117	1	Proton donor By similarity
	Binding site	6	1	Substrate By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	65	1	Substrate By similarity
	Binding site	84	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	25 ↔ 83		By similarity
	Disulfide bond	39 ↔ 94		By similarity
	Disulfide bond	57 ↔ 109		By similarity
	Disulfide bond	64 ↔ 71		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00686 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 58EFFFB51EFA539A	FASTA	122	13,832
	10 20 30 40 50 60 ETPAEKFQRQ HMDTEHSTAS SSNYCNLMMK ARDMTSGRCK PLNTFIHEPK SVVDAVCHQE 70 80 90 100 110 120 NVTCKNGRTN CYKSNSRLSI TNCRQTGASK YPNCQYETSN LNKQIIVACE GQYVPVHFDA YV

References

[1]	"The amino-acid sequence of kangaroo pancreatic ribonuclease." Gaastra W., Welling G.W., Beintema J.J. Eur. J. Biochem. 86:209-217(1978) [PubMed: 658039] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	NRKGR. A00833.
3D structure databases
ProteinModelPortal	P00686.
SMR	P00686. Positions 1-122.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_MACRU

Accession

Primary (citable) accession number: P00686

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program