P00685 (RNAS1_CHOHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Choloepus hoffmanni (Hoffmann's two-fingered sloth)

Taxonomic identifier

9358 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Xenarthra › Pilosa › Folivora › Megalonychidae › Choloepus

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Ribonuclease pancreatic		PRO_0000057192
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	119	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	85	1	Substrate By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	26 ↔ 84		By similarity
	Disulfide bond	40 ↔ 95		By similarity
	Disulfide bond	58 ↔ 110		By similarity
	Disulfide bond	65 ↔ 72		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00685 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A0AEF44C7DCAD6A7	FASTA	128	14,492
	10 20 30 40 50 60 KETAAMKFQR QHMDSGSSLS SSSDYCNKMM KVRNMTQESC KPVNTFVHES LQDVQAVCFQ 70 80 90 100 110 120 ENVTCKNGQQ NCHQSRSNMH ITDCRQTSGS KYPNCLYQTS NMNRHIIIAC EGNPYVPVHF DASVEDST

References

[1]	"Pancreatic ribonucleases of mammals with ruminant-like digestion. Amino-acid sequences of hippopotamus and sloth ribonucleases." Havinga J., Beintema J.J. Eur. J. Biochem. 110:131-142(1980) [PubMed: 7439154] [Abstract] Cited for: PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-34. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	NROW2. A00832.
3D structure databases
ProteinModelPortal	P00685.
SMR	P00685. Positions 1-128.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCHOT00000004388; ENSCHOP00000003867; ENSCHOG00000004379.
Phylogenomic databases
GeneTree	ENSGT00390000007840.
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_CHOHO

Accession

Primary (citable) accession number: P00685

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 31, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program