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Protein

Ribonuclease pancreatic beta-type

Gene

Rnase1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Substrate
Binding sitei38 – 381Substrate
Active sitei40 – 401Proton acceptor
Binding sitei94 – 941Substrate
Binding sitei113 – 1131Substrate
Active sitei147 – 1471Proton donor

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic beta-type (EC:3.1.27.5)
Alternative name(s):
RL1
RNase 1 gamma
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3574. Rnase1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 152127Ribonuclease pancreatic beta-typePRO_0000030940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 112
Disulfide bondi68 ↔ 123
Disulfide bondi86 ↔ 138
Disulfide bondi93 ↔ 100

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP00684.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevestigatoriP00684.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043700.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 407Combined sources
Helixi53 – 608Combined sources
Turni61 – 644Combined sources
Beta strandi65 – 673Combined sources
Beta strandi70 – 756Combined sources
Helixi79 – 835Combined sources
Helixi84 – 874Combined sources
Beta strandi100 – 1023Combined sources
Beta strandi107 – 1148Combined sources
Beta strandi125 – 1328Combined sources
Beta strandi134 – 1396Combined sources
Turni140 – 1434Combined sources
Beta strandi144 – 1518Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortaliP00684.
SMRiP00684. Positions 30-152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00684.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 735Substrate binding

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40319.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00684.
PhylomeDBiP00684.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS
60 70 80 90 100
PTYCNQMMKR QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC
110 120 130 140 150
HKSSSTLRIT DCRLKGSSKY PNCDYTTTDS QKHIIIACDG NPYVPVHFDA

SV
Length:152
Mass (Da):16,823
Last modified:July 21, 1986 - v1
Checksum:i86A53CCD7F8FCBEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00771 mRNA. No translation available.
PIRiA92356. NRRT.
RefSeqiXP_008766334.1. XM_008768112.1.
XP_008768852.1. XM_008770630.1.
UniGeneiRn.9397.

Genome annotation databases

GeneIDi103690354.
KEGGirno:103690354.
UCSCiRGD:3574. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00771 mRNA. No translation available.
PIRiA92356. NRRT.
RefSeqiXP_008766334.1. XM_008768112.1.
XP_008768852.1. XM_008770630.1.
UniGeneiRn.9397.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortaliP00684.
SMRiP00684. Positions 30-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043700.

Proteomic databases

PRIDEiP00684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi103690354.
KEGGirno:103690354.
UCSCiRGD:3574. rat.

Organism-specific databases

RGDi3574. Rnase1.

Phylogenomic databases

eggNOGiNOG40319.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00684.
PhylomeDBiP00684.

Miscellaneous databases

EvolutionaryTraceiP00684.
NextBioi13948373.
PROiP00684.

Gene expression databases

GenevestigatoriP00684.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme."
    McDonald R.J., Stary S.J., Swift G.H.
    J. Biol. Chem. 257:14582-14585(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rat pancreatic ribonuclease. II. Amino acid sequence."
    Beintema J.J., Gruber M.
    Biochim. Biophys. Acta 310:161-173(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-152.
    Tissue: Pancreas.
  3. "Rat pancreatic ribonuclease: agreement between the corrected amino acid sequence and the sequence derived from its messenger RNA."
    Beintema J.J.
    FEBS Lett. 159:191-195(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 98; 124; 126; 129 AND 131.
    Tissue: Pancreas.
  4. "The crystal structure of recombinant rat pancreatic RNase A."
    Gupta V., Muyldermans S., Wyns L., Salunke D.M.
    Proteins 35:1-12(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 30-152.
    Tissue: Pancreas.

Entry informationi

Entry nameiRNS1B_RAT
AccessioniPrimary (citable) accession number: P00684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 27, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.