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P00684

- RNS1B_RAT

UniProt

P00684 - RNS1B_RAT

Protein

Ribonuclease pancreatic beta-type

Gene

Rnase1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Substrate
    Binding sitei38 – 381Substrate
    Active sitei40 – 401Proton acceptor
    Binding sitei94 – 941Substrate
    Binding sitei113 – 1131Substrate
    Active sitei147 – 1471Proton donor

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. pancreatic ribonuclease activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease pancreatic beta-type (EC:3.1.27.5)
    Alternative name(s):
    RL1
    RNase 1 gamma
    RNase A
    Gene namesi
    Name:Rnase1
    Synonyms:Rib-1, Rib1, Rns1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3574. Rnase1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 152127Ribonuclease pancreatic beta-typePRO_0000030940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 112
    Disulfide bondi68 ↔ 123
    Disulfide bondi86 ↔ 138
    Disulfide bondi93 ↔ 100

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP00684.

    Expressioni

    Tissue specificityi

    Pancreas.

    Gene expression databases

    GenevestigatoriP00684.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000043700.

    Structurei

    Secondary structure

    1
    152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi34 – 407
    Helixi53 – 608
    Turni61 – 644
    Beta strandi65 – 673
    Beta strandi70 – 756
    Helixi79 – 835
    Helixi84 – 874
    Beta strandi100 – 1023
    Beta strandi107 – 1148
    Beta strandi125 – 1328
    Beta strandi134 – 1396
    Turni140 – 1434
    Beta strandi144 – 1518

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RRAX-ray2.50A30-152[»]
    ProteinModelPortaliP00684.
    SMRiP00684. Positions 30-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00684.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 735Substrate binding

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40319.
    HOGENOMiHOG000276883.
    HOVERGENiHBG008396.
    InParanoidiP00684.
    PhylomeDBiP00684.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiIPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view]
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiPF00074. RnaseA. 1 hit.
    [Graphical view]
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiPD000535. RNaseA. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00092. RNAse_Pc. 1 hit.
    [Graphical view]
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00684-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS    50
    PTYCNQMMKR QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC 100
    HKSSSTLRIT DCRLKGSSKY PNCDYTTTDS QKHIIIACDG NPYVPVHFDA 150
    SV 152
    Length:152
    Mass (Da):16,823
    Last modified:July 21, 1986 - v1
    Checksum:i86A53CCD7F8FCBEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00771 mRNA. No translation available.
    PIRiA92356. NRRT.
    UniGeneiRn.9397.

    Genome annotation databases

    UCSCiRGD:3574. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00771 mRNA. No translation available.
    PIRi A92356. NRRT.
    UniGenei Rn.9397.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RRA X-ray 2.50 A 30-152 [» ]
    ProteinModelPortali P00684.
    SMRi P00684. Positions 30-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000043700.

    Proteomic databases

    PRIDEi P00684.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3574. rat.

    Organism-specific databases

    RGDi 3574. Rnase1.

    Phylogenomic databases

    eggNOGi NOG40319.
    HOGENOMi HOG000276883.
    HOVERGENi HBG008396.
    InParanoidi P00684.
    PhylomeDBi P00684.

    Miscellaneous databases

    EvolutionaryTracei P00684.
    NextBioi 13948373.
    PROi P00684.

    Gene expression databases

    Genevestigatori P00684.

    Family and domain databases

    Gene3Di 3.10.130.10. 1 hit.
    InterProi IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view ]
    PANTHERi PTHR11437. PTHR11437. 1 hit.
    Pfami PF00074. RnaseA. 1 hit.
    [Graphical view ]
    PRINTSi PR00794. RIBONUCLEASE.
    ProDomi PD000535. RNaseA. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00092. RNAse_Pc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54076. SSF54076. 1 hit.
    PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme."
      McDonald R.J., Stary S.J., Swift G.H.
      J. Biol. Chem. 257:14582-14585(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Rat pancreatic ribonuclease. II. Amino acid sequence."
      Beintema J.J., Gruber M.
      Biochim. Biophys. Acta 310:161-173(1973) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-152.
      Tissue: Pancreas.
    3. "Rat pancreatic ribonuclease: agreement between the corrected amino acid sequence and the sequence derived from its messenger RNA."
      Beintema J.J.
      FEBS Lett. 159:191-195(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 98; 124; 126; 129 AND 131.
      Tissue: Pancreas.
    4. "The crystal structure of recombinant rat pancreatic RNase A."
      Gupta V., Muyldermans S., Wyns L., Salunke D.M.
      Proteins 35:1-12(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 30-152.
      Tissue: Pancreas.

    Entry informationi

    Entry nameiRNS1B_RAT
    AccessioniPrimary (citable) accession number: P00684
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3