Skip Header

Contribute Send feedback
Read comments (?) or add your own

P00684 (RNS1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease pancreatic beta-type
EC=3.1.27.5
Alternative name(s):
RL1
RNase 1 gamma
RNase A
Gene names
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.

Catalytic activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure

Monomer By similarity.

Subcellular location

Secreted.

Tissue specificity

Pancreas.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.2
Chain26 – 152127Ribonuclease pancreatic beta-type
PRO_0000030940

Regions

Region69 – 735Substrate binding

Sites

Active site401Proton acceptor
Active site1471Proton donor
Binding site351Substrate
Binding site381Substrate
Binding site941Substrate
Binding site1131Substrate

Amino acid modifications

Disulfide bond54 ↔ 112
Disulfide bond68 ↔ 123
Disulfide bond86 ↔ 138
Disulfide bond93 ↔ 100

Secondary structure

1 ...................... 152
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00684 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 86A53CCD7F8FCBEF

FASTA15216,823
        10         20         30         40         50         60 
MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS PTYCNQMMKR 

        70         80         90        100        110        120 
QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC HKSSSTLRIT DCRLKGSSKY 

       130        140        150 
PNCDYTTTDS QKHIIIACDG NPYVPVHFDA SV

« Hide

References

[1]"Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme."
McDonald R.J., Stary S.J., Swift G.H.
J. Biol. Chem. 257:14582-14585(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Rat pancreatic ribonuclease. II. Amino acid sequence."
Beintema J.J., Gruber M.
Biochim. Biophys. Acta 310:161-173(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-152.
Tissue: Pancreas.
[3]"Rat pancreatic ribonuclease: agreement between the corrected amino acid sequence and the sequence derived from its messenger RNA."
Beintema J.J.
FEBS Lett. 159:191-195(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 98; 124; 126; 129 AND 131.
Tissue: Pancreas.
[4]"The crystal structure of recombinant rat pancreatic RNase A."
Gupta V., Muyldermans S., Wyns L., Salunke D.M.
Proteins 35:1-12(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 30-152.
Tissue: Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J00771 mRNA. No translation available.
IPIIPI00211902.
PIRNRRT. A92356.
UniGeneRn.9397.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortalP00684.
SMRP00684. Positions 30-152.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000043700.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3574. rat.

Organism-specific databases

RGD3574. Rnase1.

Phylogenomic databases

eggNOGNOG40319.
HOGENOMHOG000276883.
HOVERGENHBG008396.
InParanoidP00684.
OrthoDBEOG4TMR3F.

Gene expression databases

ArrayExpressP00684.
GenevestigatorP00684.
GermOnlineENSRNOG00000031577. Rattus norvegicus.

Family and domain databases

Gene3D3.10.130.10. 1 hit.
InterProIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERPTHR11437. PTHR11437. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSPR00794. RIBONUCLEASE.
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMSSF54076. RNaseA. 1 hit.
PROSITEPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00684.
NextBio13948373.

Entry information

Entry nameRNS1B_RAT
AccessionPrimary (citable) accession number: P00684
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents