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P00684 (RNS1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease pancreatic beta-type

EC=3.1.27.5
Alternative name(s):
RL1
RNase 1 gamma
RNase A
Gene names
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.

Catalytic activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure

Monomer By similarity.

Subcellular location

Secreted.

Tissue specificity

Pancreas.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

pancreatic ribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.2
Chain26 – 152127Ribonuclease pancreatic beta-type
PRO_0000030940

Regions

Region69 – 735Substrate binding

Sites

Active site401Proton acceptor
Active site1471Proton donor
Binding site351Substrate
Binding site381Substrate
Binding site941Substrate
Binding site1131Substrate

Amino acid modifications

Disulfide bond54 ↔ 112
Disulfide bond68 ↔ 123
Disulfide bond86 ↔ 138
Disulfide bond93 ↔ 100

Secondary structure

...................... 152
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00684 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 86A53CCD7F8FCBEF

FASTA15216,823
        10         20         30         40         50         60 
MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS PTYCNQMMKR 

        70         80         90        100        110        120 
QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC HKSSSTLRIT DCRLKGSSKY 

       130        140        150 
PNCDYTTTDS QKHIIIACDG NPYVPVHFDA SV 

« Hide

References

[1]"Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme."
McDonald R.J., Stary S.J., Swift G.H.
J. Biol. Chem. 257:14582-14585(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Rat pancreatic ribonuclease. II. Amino acid sequence."
Beintema J.J., Gruber M.
Biochim. Biophys. Acta 310:161-173(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-152.
Tissue: Pancreas.
[3]"Rat pancreatic ribonuclease: agreement between the corrected amino acid sequence and the sequence derived from its messenger RNA."
Beintema J.J.
FEBS Lett. 159:191-195(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 98; 124; 126; 129 AND 131.
Tissue: Pancreas.
[4]"The crystal structure of recombinant rat pancreatic RNase A."
Gupta V., Muyldermans S., Wyns L., Salunke D.M.
Proteins 35:1-12(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 30-152.
Tissue: Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00771 mRNA. No translation available.
PIRNRRT. A92356.
UniGeneRn.9397.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortalP00684.
SMRP00684. Positions 30-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000043700.

Proteomic databases

PRIDEP00684.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3574. rat.

Organism-specific databases

RGD3574. Rnase1.

Phylogenomic databases

eggNOGNOG40319.
HOGENOMHOG000276883.
HOVERGENHBG008396.
InParanoidP00684.
PhylomeDBP00684.

Gene expression databases

GenevestigatorP00684.

Family and domain databases

Gene3D3.10.130.10. 1 hit.
InterProIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERPTHR11437. PTHR11437. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSPR00794. RIBONUCLEASE.
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMSSF54076. SSF54076. 1 hit.
PROSITEPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00684.
NextBio13948373.
PROP00684.

Entry information

Entry nameRNS1B_RAT
AccessionPrimary (citable) accession number: P00684
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references