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Protein

Ribonuclease pancreatic beta-type

Gene

Rnase1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Substrate1
Binding sitei38Substrate1
Active sitei40Proton acceptor1
Binding sitei94Substrate1
Binding sitei113Substrate1
Active sitei147Proton donor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic beta-type (EC:3.1.27.5)
Alternative name(s):
RL1
RNase 1 gamma
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi3574. Rnase1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003094026 – 152Ribonuclease pancreatic beta-typeAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 112
Disulfide bondi68 ↔ 123
Disulfide bondi86 ↔ 138
Disulfide bondi93 ↔ 100

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP00684.
PRIDEiP00684.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevisibleiP00684. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043700.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 40Combined sources7
Helixi53 – 60Combined sources8
Turni61 – 64Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi70 – 75Combined sources6
Helixi79 – 83Combined sources5
Helixi84 – 87Combined sources4
Beta strandi100 – 102Combined sources3
Beta strandi107 – 114Combined sources8
Beta strandi125 – 132Combined sources8
Beta strandi134 – 139Combined sources6
Turni140 – 143Combined sources4
Beta strandi144 – 151Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortaliP00684.
SMRiP00684.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00684.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 73Substrate binding5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYEN. Eukaryota.
ENOG41113F1. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00684.
KOiK01168.
OMAiNCAYRTS.
OrthoDBiEOG091G15X3.
PhylomeDBiP00684.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS
60 70 80 90 100
PTYCNQMMKR QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC
110 120 130 140 150
HKSSSTLRIT DCRLKGSSKY PNCDYTTTDS QKHIIIACDG NPYVPVHFDA

SV
Length:152
Mass (Da):16,823
Last modified:July 21, 1986 - v1
Checksum:i86A53CCD7F8FCBEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00771 mRNA. No translation available.
PIRiA92356. NRRT.
RefSeqiXP_008768852.1. XM_008770630.1.
UniGeneiRn.9397.

Genome annotation databases

EnsembliENSRNOT00000086427; ENSRNOP00000068653; ENSRNOG00000053633.
GeneIDi103690354.
KEGGirno:103690354.
UCSCiRGD:3574. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00771 mRNA. No translation available.
PIRiA92356. NRRT.
RefSeqiXP_008768852.1. XM_008770630.1.
UniGeneiRn.9397.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortaliP00684.
SMRiP00684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043700.

Proteomic databases

PaxDbiP00684.
PRIDEiP00684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000086427; ENSRNOP00000068653; ENSRNOG00000053633.
GeneIDi103690354.
KEGGirno:103690354.
UCSCiRGD:3574. rat.

Organism-specific databases

RGDi3574. Rnase1.

Phylogenomic databases

eggNOGiENOG410IYEN. Eukaryota.
ENOG41113F1. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00684.
KOiK01168.
OMAiNCAYRTS.
OrthoDBiEOG091G15X3.
PhylomeDBiP00684.

Miscellaneous databases

EvolutionaryTraceiP00684.
PROiP00684.

Gene expression databases

GenevisibleiP00684. RN.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNS1B_RAT
AccessioniPrimary (citable) accession number: P00684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.