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P00684

- RNS1B_RAT

UniProt

P00684 - RNS1B_RAT

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Protein
Ribonuclease pancreatic beta-type
Gene
Rnase1, Rib-1, Rib1, Rns1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Substrate
Binding sitei38 – 381Substrate
Active sitei40 – 401Proton acceptor
Binding sitei94 – 941Substrate
Binding sitei113 – 1131Substrate
Active sitei147 – 1471Proton donor

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic beta-type (EC:3.1.27.5)
Alternative name(s):
RL1
RNase 1 gamma
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3574. Rnase1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 Publication
Add
BLAST
Chaini26 – 152127Ribonuclease pancreatic beta-type
PRO_0000030940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 112
Disulfide bondi68 ↔ 123
Disulfide bondi86 ↔ 138
Disulfide bondi93 ↔ 100

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP00684.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevestigatoriP00684.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043700.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 407
Helixi53 – 608
Turni61 – 644
Beta strandi65 – 673
Beta strandi70 – 756
Helixi79 – 835
Helixi84 – 874
Beta strandi100 – 1023
Beta strandi107 – 1148
Beta strandi125 – 1328
Beta strandi134 – 1396
Turni140 – 1434
Beta strandi144 – 1518

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRAX-ray2.50A30-152[»]
ProteinModelPortaliP00684.
SMRiP00684. Positions 30-152.

Miscellaneous databases

EvolutionaryTraceiP00684.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 735Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40319.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00684.
PhylomeDBiP00684.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00684-1 [UniParc]FASTAAdd to Basket

« Hide

MGLEKSLFLF SLLVLVLGWV QPSLGGESRE SSADKFKRQH MDTEGPSKSS    50
PTYCNQMMKR QGMTKGSCKP VNTFVHEPLE DVQAICSQGQ VTCKNGRNNC 100
HKSSSTLRIT DCRLKGSSKY PNCDYTTTDS QKHIIIACDG NPYVPVHFDA 150
SV 152
Length:152
Mass (Da):16,823
Last modified:July 21, 1986 - v1
Checksum:i86A53CCD7F8FCBEF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00771 mRNA. No translation available.
PIRiA92356. NRRT.
UniGeneiRn.9397.

Genome annotation databases

UCSCiRGD:3574. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00771 mRNA. No translation available.
PIRi A92356. NRRT.
UniGenei Rn.9397.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RRA X-ray 2.50 A 30-152 [» ]
ProteinModelPortali P00684.
SMRi P00684. Positions 30-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000043700.

Proteomic databases

PRIDEi P00684.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3574. rat.

Organism-specific databases

RGDi 3574. Rnase1.

Phylogenomic databases

eggNOGi NOG40319.
HOGENOMi HOG000276883.
HOVERGENi HBG008396.
InParanoidi P00684.
PhylomeDBi P00684.

Miscellaneous databases

EvolutionaryTracei P00684.
NextBioi 13948373.
PROi P00684.

Gene expression databases

Genevestigatori P00684.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme."
    McDonald R.J., Stary S.J., Swift G.H.
    J. Biol. Chem. 257:14582-14585(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rat pancreatic ribonuclease. II. Amino acid sequence."
    Beintema J.J., Gruber M.
    Biochim. Biophys. Acta 310:161-173(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-152.
    Tissue: Pancreas.
  3. "Rat pancreatic ribonuclease: agreement between the corrected amino acid sequence and the sequence derived from its messenger RNA."
    Beintema J.J.
    FEBS Lett. 159:191-195(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 98; 124; 126; 129 AND 131.
    Tissue: Pancreas.
  4. "The crystal structure of recombinant rat pancreatic RNase A."
    Gupta V., Muyldermans S., Wyns L., Salunke D.M.
    Proteins 35:1-12(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 30-152.
    Tissue: Pancreas.

Entry informationi

Entry nameiRNS1B_RAT
AccessioniPrimary (citable) accession number: P00684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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