Reviewed, UniProtKB/Swiss-Prot P00683 (RNAS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:	Rnase1
Synonyms:	Rib-1, Rib1, Rns1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.3
	Chain	26 – 149	124	Ribonuclease pancreatic		PRO_0000030929
Regions
	Region	66 – 70	5	Substrate binding By similarity
Sites
	Active site	37	1	Proton acceptor By similarity
	Active site	144	1	Proton donor By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 109		By similarity
	Disulfide bond	65 ↔ 120		By similarity
	Disulfide bond	83 ↔ 135		By similarity
	Disulfide bond	90 ↔ 97		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00683-1 [UniParc]. Last modified May 1, 1991. Version 2. Checksum: 2FC1E0A50D245162	FASTA	149	16,820
	10 20 30 40 50 60 MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY CNQMMKRRDM 70 80 90 100 110 120 TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS SSALHITDCH LKGNSKYPNC 130 140 DYKTTQYQKH IIVACEGNPY VPVHFDATV

References

[1]	"Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease." Schueller C., Nijssen H.M.J., Kok R., Beintema J.J. Mol. Biol. Evol. 7:29-44(1990) [PubMed: 2299980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland." Samuelson L.C., Wiebauer K., Howard G., Schmid R.M., Koeplin D., Meisler M.H. Nucleic Acids Res. 19:6935-6941(1991) [PubMed: 1840677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C3H/HeJ. Tissue: Spleen.
[3]	"The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases." Lenstra J.A., Beintema J.J. Eur. J. Biochem. 98:399-408(1979) [PubMed: 556267] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-149. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27814 mRNA. Translation: AAA40060.1. X60103 Genomic DNA. Translation: CAA42697.1.
IPI	IPI00133541.
PIR	NRMS. A34090.
UniGene	Mm.235538
3D structure databases
HSSP	HSSP built from PDB template 1SRN based on UniProtKB P00656.
SMR	P00683. Positions 27-149.
ModBase	Search...
Protein-protein interaction databases
STRING	P00683.
Proteomic databases
PRIDE	P00683.
Genome annotation databases
Ensembl	ENSMUST00000080126; ENSMUSP00000079025; ENSMUSG00000035896; Mus musculus. [Genome view]
UCSC	F12F6.3.2. c. elegans.
Organism-specific databases
MGI	MGI:97919. Rnase1.
Phylogenomic databases
HOGENOM	P00683.
HOVERGEN	P00683.
Enzyme and pathway databases
BRENDA	3.1.27.5. 244.
Gene expression databases
ArrayExpress	P00683.
Bgee	P00683.
CleanEx	MM_RNASE1.
Genevestigator	P00683.
GermOnline	ENSMUSG00000035896. Mus musculus.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

RNAS1_MOUSE

Accession

Primary (citable) accession number: P00683

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	May 1, 1991
Last modified:	October 13, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)