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P00683

- RNAS1_MOUSE

UniProt

P00683 - RNAS1_MOUSE

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Protein

Ribonuclease pancreatic

Gene

Rnase1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321SubstrateBy similarity
Binding sitei35 – 351SubstrateBy similarity
Active sitei37 – 371Proton acceptorBy similarity
Binding sitei91 – 911SubstrateBy similarity
Active sitei144 – 1441Proton donorBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic (EC:3.1.27.5)
Alternative name(s):
RNase 1
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97919. Rnase1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 149124Ribonuclease pancreaticPRO_0000030929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 109By similarity
Disulfide bondi65 ↔ 120By similarity
Disulfide bondi83 ↔ 135By similarity
Disulfide bondi90 ↔ 97By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00683.
PaxDbiP00683.
PRIDEiP00683.

PTM databases

PhosphoSiteiP00683.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

CleanExiMM_RNASE1.
GenevestigatoriP00683.

Interactioni

Subunit structurei

Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein (By similarity).By similarity

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 379
Helixi50 – 578
Beta strandi61 – 644
Beta strandi67 – 726
Helixi76 – 805
Helixi81 – 844
Beta strandi85 – 884
Beta strandi97 – 993
Beta strandi104 – 1118
Beta strandi122 – 13615
Turni137 – 1404
Beta strandi141 – 1499

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortaliP00683.
SMRiP00683. Positions 26-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni66 – 705Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40319.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00683.
PhylomeDBiP00683.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00683-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY
60 70 80 90 100
CNQMMKRRDM TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS
110 120 130 140
SSALHITDCH LKGNSKYPNC DYKTTQYQKH IIVACEGNPY VPVHFDATV
Length:149
Mass (Da):16,820
Last modified:May 1, 1991 - v2
Checksum:i2FC1E0A50D245162
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27814 mRNA. Translation: AAA40060.1.
X60103 Genomic DNA. Translation: CAA42697.1.
CCDSiCCDS27038.1.
PIRiA34090. NRMS.
UniGeneiMm.235538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27814 mRNA. Translation: AAA40060.1 .
X60103 Genomic DNA. Translation: CAA42697.1 .
CCDSi CCDS27038.1.
PIRi A34090. NRMS.
UniGenei Mm.235538.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TSR X-ray 2.20 A/B/C/D 26-149 [» ]
ProteinModelPortali P00683.
SMRi P00683. Positions 26-149.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P00683.

Proteomic databases

MaxQBi P00683.
PaxDbi P00683.
PRIDEi P00683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97919. Rnase1.

Phylogenomic databases

eggNOGi NOG40319.
HOGENOMi HOG000276883.
HOVERGENi HBG008396.
InParanoidi P00683.
PhylomeDBi P00683.

Miscellaneous databases

ChiTaRSi RNASE1. mouse.
PROi P00683.
SOURCEi Search...

Gene expression databases

CleanExi MM_RNASE1.
Genevestigatori P00683.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease."
    Schueller C., Nijssen H.M.J., Kok R., Beintema J.J.
    Mol. Biol. Evol. 7:29-44(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland."
    Samuelson L.C., Wiebauer K., Howard G., Schmid R.M., Koeplin D., Meisler M.H.
    Nucleic Acids Res. 19:6935-6941(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C3H/HeJ.
    Tissue: Spleen.
  3. "The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases."
    Lenstra J.A., Beintema J.J.
    Eur. J. Biochem. 98:399-408(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-149.
    Tissue: Pancreas.

Entry informationi

Entry nameiRNAS1_MOUSE
AccessioniPrimary (citable) accession number: P00683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1991
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3