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P00683

- RNAS1_MOUSE

UniProt

P00683 - RNAS1_MOUSE

Protein

Ribonuclease pancreatic

Gene

Rnase1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Binding sitei35 – 351SubstrateBy similarity
    Active sitei37 – 371Proton acceptorBy similarity
    Binding sitei91 – 911SubstrateBy similarity
    Active sitei144 – 1441Proton donorBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. pancreatic ribonuclease activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease pancreatic (EC:3.1.27.5)
    Alternative name(s):
    RNase 1
    RNase A
    Gene namesi
    Name:Rnase1
    Synonyms:Rib-1, Rib1, Rns1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97919. Rnase1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 149124Ribonuclease pancreaticPRO_0000030929Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi51 ↔ 109By similarity
    Disulfide bondi65 ↔ 120By similarity
    Disulfide bondi83 ↔ 135By similarity
    Disulfide bondi90 ↔ 97By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP00683.
    PaxDbiP00683.
    PRIDEiP00683.

    PTM databases

    PhosphoSiteiP00683.

    Expressioni

    Tissue specificityi

    Pancreas.

    Gene expression databases

    CleanExiMM_RNASE1.
    GenevestigatoriP00683.

    Interactioni

    Subunit structurei

    Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.By similarity

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 379
    Helixi50 – 578
    Beta strandi61 – 644
    Beta strandi67 – 726
    Helixi76 – 805
    Helixi81 – 844
    Beta strandi85 – 884
    Beta strandi97 – 993
    Beta strandi104 – 1118
    Beta strandi122 – 13615
    Turni137 – 1404
    Beta strandi141 – 1499

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TSRX-ray2.20A/B/C/D26-149[»]
    ProteinModelPortaliP00683.
    SMRiP00683. Positions 26-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni66 – 705Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40319.
    HOGENOMiHOG000276883.
    HOVERGENiHBG008396.
    InParanoidiP00683.
    PhylomeDBiP00683.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiIPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view]
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiPF00074. RnaseA. 1 hit.
    [Graphical view]
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiPD000535. RNaseA. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00092. RNAse_Pc. 1 hit.
    [Graphical view]
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY    50
    CNQMMKRRDM TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS 100
    SSALHITDCH LKGNSKYPNC DYKTTQYQKH IIVACEGNPY VPVHFDATV 149
    Length:149
    Mass (Da):16,820
    Last modified:May 1, 1991 - v2
    Checksum:i2FC1E0A50D245162
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27814 mRNA. Translation: AAA40060.1.
    X60103 Genomic DNA. Translation: CAA42697.1.
    CCDSiCCDS27038.1.
    PIRiA34090. NRMS.
    UniGeneiMm.235538.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27814 mRNA. Translation: AAA40060.1 .
    X60103 Genomic DNA. Translation: CAA42697.1 .
    CCDSi CCDS27038.1.
    PIRi A34090. NRMS.
    UniGenei Mm.235538.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TSR X-ray 2.20 A/B/C/D 26-149 [» ]
    ProteinModelPortali P00683.
    SMRi P00683. Positions 26-149.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P00683.

    Proteomic databases

    MaxQBi P00683.
    PaxDbi P00683.
    PRIDEi P00683.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:97919. Rnase1.

    Phylogenomic databases

    eggNOGi NOG40319.
    HOGENOMi HOG000276883.
    HOVERGENi HBG008396.
    InParanoidi P00683.
    PhylomeDBi P00683.

    Miscellaneous databases

    ChiTaRSi RNASE1. mouse.
    PROi P00683.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_RNASE1.
    Genevestigatori P00683.

    Family and domain databases

    Gene3Di 3.10.130.10. 1 hit.
    InterProi IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view ]
    PANTHERi PTHR11437. PTHR11437. 1 hit.
    Pfami PF00074. RnaseA. 1 hit.
    [Graphical view ]
    PRINTSi PR00794. RIBONUCLEASE.
    ProDomi PD000535. RNaseA. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00092. RNAse_Pc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54076. SSF54076. 1 hit.
    PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease."
      Schueller C., Nijssen H.M.J., Kok R., Beintema J.J.
      Mol. Biol. Evol. 7:29-44(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland."
      Samuelson L.C., Wiebauer K., Howard G., Schmid R.M., Koeplin D., Meisler M.H.
      Nucleic Acids Res. 19:6935-6941(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C3H/HeJ.
      Tissue: Spleen.
    3. "The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases."
      Lenstra J.A., Beintema J.J.
      Eur. J. Biochem. 98:399-408(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-149.
      Tissue: Pancreas.

    Entry informationi

    Entry nameiRNAS1_MOUSE
    AccessioniPrimary (citable) accession number: P00683
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3