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P00683 (RNAS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease pancreatic

EC=3.1.27.5
Alternative name(s):
RNase 1
RNase A
Gene names
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length149 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.

Catalytic activity

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Subunit structure

Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.

Subcellular location

Secreted.

Tissue specificity

Pancreas.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

pancreatic ribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.3
Chain26 – 149124Ribonuclease pancreatic
PRO_0000030929

Regions

Region66 – 705Substrate binding By similarity

Sites

Active site371Proton acceptor By similarity
Active site1441Proton donor By similarity
Binding site321Substrate By similarity
Binding site351Substrate By similarity
Binding site911Substrate By similarity

Amino acid modifications

Disulfide bond51 ↔ 109 By similarity
Disulfide bond65 ↔ 120 By similarity
Disulfide bond83 ↔ 135 By similarity
Disulfide bond90 ↔ 97 By similarity

Secondary structure

.................... 149
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00683 [UniParc].

Last modified May 1, 1991. Version 2.
Checksum: 2FC1E0A50D245162

FASTA14916,820
        10         20         30         40         50         60 
MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY CNQMMKRRDM 

        70         80         90        100        110        120 
TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS SSALHITDCH LKGNSKYPNC 

       130        140 
DYKTTQYQKH IIVACEGNPY VPVHFDATV 

« Hide

References

[1]"Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease."
Schueller C., Nijssen H.M.J., Kok R., Beintema J.J.
Mol. Biol. Evol. 7:29-44(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland."
Samuelson L.C., Wiebauer K., Howard G., Schmid R.M., Koeplin D., Meisler M.H.
Nucleic Acids Res. 19:6935-6941(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C3H/HeJ.
Tissue: Spleen.
[3]"The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases."
Lenstra J.A., Beintema J.J.
Eur. J. Biochem. 98:399-408(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-149.
Tissue: Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27814 mRNA. Translation: AAA40060.1.
X60103 Genomic DNA. Translation: CAA42697.1.
CCDSCCDS27038.1.
PIRNRMS. A34090.
UniGeneMm.235538.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortalP00683.
SMRP00683. Positions 26-149.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP00683.

Proteomic databases

MaxQBP00683.
PaxDbP00683.
PRIDEP00683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:97919. Rnase1.

Phylogenomic databases

eggNOGNOG40319.
HOGENOMHOG000276883.
HOVERGENHBG008396.
InParanoidP00683.
PhylomeDBP00683.

Gene expression databases

CleanExMM_RNASE1.
GenevestigatorP00683.

Family and domain databases

Gene3D3.10.130.10. 1 hit.
InterProIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERPTHR11437. PTHR11437. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSPR00794. RIBONUCLEASE.
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMSSF54076. SSF54076. 1 hit.
PROSITEPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRNASE1. mouse.
PROP00683.
SOURCESearch...

Entry information

Entry nameRNAS1_MOUSE
AccessionPrimary (citable) accession number: P00683
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1991
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot