Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease pancreatic

Gene

Rnase1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Binding sitei35SubstrateBy similarity1
Active sitei37Proton acceptorBy similarity1
Binding sitei91SubstrateBy similarity1
Active sitei144Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic (EC:3.1.27.5)
Alternative name(s):
RNase 1
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97919. Rnase1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003092926 – 149Ribonuclease pancreaticAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 109By similarity
Disulfide bondi65 ↔ 120By similarity
Disulfide bondi83 ↔ 135By similarity
Disulfide bondi90 ↔ 97By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00683.
PaxDbiP00683.
PeptideAtlasiP00683.
PRIDEiP00683.

PTM databases

PhosphoSitePlusiP00683.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

CleanExiMM_RNASE1.

Interactioni

Subunit structurei

Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079025.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 37Combined sources9
Helixi50 – 57Combined sources8
Beta strandi61 – 64Combined sources4
Beta strandi67 – 72Combined sources6
Helixi76 – 80Combined sources5
Helixi81 – 84Combined sources4
Beta strandi85 – 88Combined sources4
Beta strandi97 – 99Combined sources3
Beta strandi104 – 111Combined sources8
Beta strandi122 – 136Combined sources15
Turni137 – 140Combined sources4
Beta strandi141 – 149Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortaliP00683.
SMRiP00683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 70Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYEN. Eukaryota.
ENOG41113F1. LUCA.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00683.
PhylomeDBiP00683.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY
60 70 80 90 100
CNQMMKRRDM TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS
110 120 130 140
SSALHITDCH LKGNSKYPNC DYKTTQYQKH IIVACEGNPY VPVHFDATV
Length:149
Mass (Da):16,820
Last modified:May 1, 1991 - v2
Checksum:i2FC1E0A50D245162
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27814 mRNA. Translation: AAA40060.1.
X60103 Genomic DNA. Translation: CAA42697.1.
CCDSiCCDS27038.1.
PIRiA34090. NRMS.
UniGeneiMm.235538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27814 mRNA. Translation: AAA40060.1.
X60103 Genomic DNA. Translation: CAA42697.1.
CCDSiCCDS27038.1.
PIRiA34090. NRMS.
UniGeneiMm.235538.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortaliP00683.
SMRiP00683.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079025.

PTM databases

PhosphoSitePlusiP00683.

Proteomic databases

MaxQBiP00683.
PaxDbiP00683.
PeptideAtlasiP00683.
PRIDEiP00683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97919. Rnase1.

Phylogenomic databases

eggNOGiENOG410IYEN. Eukaryota.
ENOG41113F1. LUCA.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00683.
PhylomeDBiP00683.

Miscellaneous databases

ChiTaRSiRnase1. mouse.
PROiP00683.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RNASE1.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNAS1_MOUSE
AccessioniPrimary (citable) accession number: P00683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.