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P00683

- RNAS1_MOUSE

UniProt

P00683 - RNAS1_MOUSE

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Protein

Ribonuclease pancreatic

Gene
Rnase1, Rib-1, Rib1, Rns1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA By similarity.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321Substrate By similarity
Binding sitei35 – 351Substrate By similarity
Active sitei37 – 371Proton acceptor By similarity
Binding sitei91 – 911Substrate By similarity
Active sitei144 – 1441Proton donor By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic (EC:3.1.27.5)
Alternative name(s):
RNase 1
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97919. Rnase1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 149124Ribonuclease pancreaticPRO_0000030929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 109 By similarity
Disulfide bondi65 ↔ 120 By similarity
Disulfide bondi83 ↔ 135 By similarity
Disulfide bondi90 ↔ 97 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00683.
PaxDbiP00683.
PRIDEiP00683.

PTM databases

PhosphoSiteiP00683.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

CleanExiMM_RNASE1.
GenevestigatoriP00683.

Interactioni

Subunit structurei

Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 379
Helixi50 – 578
Beta strandi61 – 644
Beta strandi67 – 726
Helixi76 – 805
Helixi81 – 844
Beta strandi85 – 884
Beta strandi97 – 993
Beta strandi104 – 1118
Beta strandi122 – 13615
Turni137 – 1404
Beta strandi141 – 1499

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortaliP00683.
SMRiP00683. Positions 26-149.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni66 – 705Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40319.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP00683.
PhylomeDBiP00683.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00683-1 [UniParc]FASTAAdd to Basket

« Hide

MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY    50
CNQMMKRRDM TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS 100
SSALHITDCH LKGNSKYPNC DYKTTQYQKH IIVACEGNPY VPVHFDATV 149
Length:149
Mass (Da):16,820
Last modified:May 1, 1991 - v2
Checksum:i2FC1E0A50D245162
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27814 mRNA. Translation: AAA40060.1.
X60103 Genomic DNA. Translation: CAA42697.1.
CCDSiCCDS27038.1.
PIRiA34090. NRMS.
UniGeneiMm.235538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27814 mRNA. Translation: AAA40060.1 .
X60103 Genomic DNA. Translation: CAA42697.1 .
CCDSi CCDS27038.1.
PIRi A34090. NRMS.
UniGenei Mm.235538.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TSR X-ray 2.20 A/B/C/D 26-149 [» ]
ProteinModelPortali P00683.
SMRi P00683. Positions 26-149.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P00683.

Proteomic databases

MaxQBi P00683.
PaxDbi P00683.
PRIDEi P00683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97919. Rnase1.

Phylogenomic databases

eggNOGi NOG40319.
HOGENOMi HOG000276883.
HOVERGENi HBG008396.
InParanoidi P00683.
PhylomeDBi P00683.

Miscellaneous databases

ChiTaRSi RNASE1. mouse.
PROi P00683.
SOURCEi Search...

Gene expression databases

CleanExi MM_RNASE1.
Genevestigatori P00683.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease."
    Schueller C., Nijssen H.M.J., Kok R., Beintema J.J.
    Mol. Biol. Evol. 7:29-44(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland."
    Samuelson L.C., Wiebauer K., Howard G., Schmid R.M., Koeplin D., Meisler M.H.
    Nucleic Acids Res. 19:6935-6941(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C3H/HeJ.
    Tissue: Spleen.
  3. "The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases."
    Lenstra J.A., Beintema J.J.
    Eur. J. Biochem. 98:399-408(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-149.
    Tissue: Pancreas.

Entry informationi

Entry nameiRNAS1_MOUSE
AccessioniPrimary (citable) accession number: P00683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1991
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi