P00674 (RNAS1_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Equus caballus (Horse) [Complete proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Ribonuclease pancreatic		PRO_0000057200
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	119	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	85	1	Substrate By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Ref.1
	Glycosylation	62	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	26 ↔ 84		By similarity
	Disulfide bond	40 ↔ 95		By similarity
	Disulfide bond	58 ↔ 110		By similarity
	Disulfide bond	65 ↔ 72		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00674 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: A06727414097C1DD	FASTA	128	14,374
	10 20 30 40 50 60 KESPAMKFER QHMDSGSTSS SNPTYCNQMM KRRNMTQGWC KPVNTFVHEP LADVQAICLQ 70 80 90 100 110 120 KNITCKNGQS NCYQSSSSMH ITDCRLTSGS KYPNCAYQTS QKERHIIVAC EGNPYVPVHF DASVEVST

References

[1]	"Horse pancreatic ribonuclease." Scheffer A.J., Beintema J.J. Eur. J. Biochem. 46:221-233(1974) [PubMed: 4852291] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-34 AND ASN-62.
[2]	"Mammalian ribonucleases. The absence of a glycosylated Asn-Pro-Thr sequence in horse ribonuclease and the presence of tryptophan at position 39 in horse and dromedary ribonuclease." Beintema J.J. FEBS Lett. 185:115-120(1985) [PubMed: 3922790] [Abstract] Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	NRHO. A91340.
3D structure databases
ProteinModelPortal	P00674.
SMR	P00674. Positions 1-125.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000007840.
HOVERGEN	HBG008396.
InParanoid	P00674.
OrthoDB	EOG4TMR3F.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_HORSE

Accession

Primary (citable) accession number: P00674

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	September 21, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program