Reviewed, UniProtKB/Swiss-Prot P00672 (RNAS1_HIPAM)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Hippopotamus amphibius (Hippopotamus)

Taxonomic identifier

9833 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Hippopotamidae › Hippopotamus

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	Ribonuclease pancreatic		PRO_0000057199
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	119	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	85	1	Substrate By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	26 ↔ 84		By similarity
	Disulfide bond	40 ↔ 95		By similarity
	Disulfide bond	58 ↔ 110		By similarity
	Disulfide bond	65 ↔ 72		By similarity
Natural variations
	Natural variant	37	1	Q → K in 50%, this form is not glycosylated. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00672-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 496CFB9B3DE0D3AB	FASTA	124	14,025
	10 20 30 40 50 60 KETAAEKFQR QHMDTSSSLS NDSNYCNQMM VRRNMTQDRC KPVNTFVHES EADVKAVCSQ 70 80 90 100 110 120 KNVTCKNGQT NCYQSNSTMH ITDCRETGSS KYPNCAYKTS QLQKHIIVAC EGDPYVPVHY DASV

References

[1]	"Pancreatic ribonucleases of mammals with ruminant-like digestion. Amino-acid sequences of hippopotamus and sloth ribonucleases." Havinga J., Beintema J.J. Eur. J. Biochem. 110:131-142(1980) [PubMed: 7439154] [Abstract] Cited for: PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-34, VARIANT LYS-37.

Cross-references

Sequence databases
PIR	NRHP. A00817.
3D structure databases
HSSP	HSSP built from PDB template 1SSB based on UniProtKB P00656.
SMR	P00672. Positions 1-124.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00672.
Enzyme and pathway databases
BRENDA	3.1.27.5. 307290.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_HIPAM

Accession

Primary (citable) accession number: P00672

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)