ID   RNAS1_RANTA             Reviewed;         124 AA.
AC   P00666;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   14-DEC-2011, entry version 67.
DE   RecName: Full=Ribonuclease pancreatic;
DE            EC=3.1.27.5;
DE   AltName: Full=RNase 1;
DE   AltName: Full=RNase A;
GN   Name=RNASE1; Synonyms=RNS1;
OS   Rangifer tarandus (Reindeer) (Cervus tarandus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Cervidae; Odocoileinae; Rangifer.
OX   NCBI_TaxID=9870;
RN   [1]
RP   PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Pancreas;
RX   MEDLINE=76003215; PubMed=1157925; DOI=10.1016/0014-5793(75)80120-7;
RA   Leijenaar-Van den Berg G., Beintema J.J.;
RT   "The amino acid sequences of reindeer, moose and fallow deer
RT   pancreatic ribonucleases.";
RL   FEBS Lett. 56:101-107(1975).
CC   -!- FUNCTION: Endonuclease that catalyzes the cleavage of RNA on the
CC       3' side of pyrimidine nucleotides. Acts on single stranded and
CC       double stranded RNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to nucleoside 3'-
CC       phosphates and 3'-phosphooligonucleotides ending in Cp or Up with
CC       2',3'-cyclic phosphate intermediates.
CC   -!- SUBUNIT: Monomer. Interacts with and forms tight 1:1 complexes
CC       with RNH1. Dimerization of two such complexes may occur.
CC       Interaction with RNH1 inhibits this protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Pancreas.
CC   -!- SIMILARITY: Belongs to the pancreatic ribonuclease family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   PIR; B91418; NRDEN.
DR   ProteinModelPortal; P00666; -.
DR   SMR; P00666; 1-124.
DR   HOVERGEN; HBG008396; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0004522; F:pancreatic ribonuclease activity; IEA:EC.
DR   InterPro; IPR001427; RNaseA.
DR   InterPro; IPR023411; RNaseA_AS.
DR   InterPro; IPR023412; RNaseA_domain.
DR   Gene3D; G3DSA:3.10.130.10; RNaseA; 1.
DR   PANTHER; PTHR11437; RNaseA; 1.
DR   Pfam; PF00074; RnaseA; 1.
DR   PRINTS; PR00794; RIBONUCLEASE.
DR   ProDom; PD000535; RNaseA; 1.
DR   SMART; SM00092; RNAse_Pc; 1.
DR   SUPFAM; SSF54076; RNaseA; 1.
DR   PROSITE; PS00127; RNASE_PANCREATIC; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Endonuclease; Hydrolase;
KW   Nuclease; Secreted.
FT   CHAIN         1    124       Ribonuclease pancreatic.
FT                                /FTId=PRO_0000057213.
FT   REGION       41     45       Substrate binding (By similarity).
FT   ACT_SITE     12     12       Proton acceptor (By similarity).
FT   ACT_SITE    119    119       Proton donor (By similarity).
FT   BINDING       7      7       Substrate (By similarity).
FT   BINDING      10     10       Substrate (By similarity).
FT   BINDING      66     66       Substrate (By similarity).
FT   BINDING      85     85       Substrate (By similarity).
FT   DISULFID     26     84       By similarity.
FT   DISULFID     40     95       By similarity.
FT   DISULFID     58    110       By similarity.
FT   DISULFID     65     72       By similarity.
SQ   SEQUENCE   124 AA;  13759 MW;  CE56B8BB9A87CD54 CRC64;
     KESAAAKFER QHMDPSPSSA SSSNYCNQMM QSRDLTQDRC KPVNTFVHES LADVQAVCFQ
     KNVACKNGQS NCYQSNSAMH ITDCRETGSS KYPNCVYKTT QAEKHIIVAC EGNPYVPVHF
     DASV
//