Reviewed, UniProtKB/Swiss-Prot P00663 (RNAS1_CEREL)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Cervus elaphus (Red deer)

Taxonomic identifier

9860 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Cervidae › Cervinae › Cervus

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	Ribonuclease pancreatic		PRO_0000057190
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	119	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	85	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	26 ↔ 84		By similarity
	Disulfide bond	40 ↔ 95		By similarity
	Disulfide bond	58 ↔ 110		By similarity
	Disulfide bond	65 ↔ 72		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00663-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 330F86D52B3DD71B	FASTA	124	13,777
	10 20 30 40 50 60 KESAAAKFER QHMDPSTSSA SSSNYCNQMM QSRKMTQDRC KPVNTFVHES LADVQAVCFQ 70 80 90 100 110 120 KNVACKNGQS NCYQSNSAMH ITDCRESGNS KYPNCVYKAT QAEKHIIVAC EGNPYVPVHF DASV

References

[1]	"Amino-acid sequences of red-deer and roe-deer pancreatic ribonucleases." Zwiers H., Scheffer A.J., Beintema J.J. Eur. J. Biochem. 36:569-574(1973) [PubMed: 4738402] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.
[2]	"Reinvestigation of the primary structures of red deer and roe deer pancreatic ribonuclease and proline sites in mammalian ribonucleases." Oosterhuis S., Welling G.W., Gaastra W., Beintema J.J. Biochim. Biophys. Acta 490:523-529(1977) [PubMed: 836889] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
PIR	NRDER. B90613.
3D structure databases
HSSP	HSSP built from PDB template 1SRN based on UniProtKB P00656.
SMR	P00663. Positions 1-124.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00663.
Enzyme and pathway databases
BRENDA	3.1.27.5. 289563.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_CEREL

Accession

Primary (citable) accession number: P00663

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)