Reviewed, UniProtKB/Swiss-Prot P00662 (RNAS1_GIRCA)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Giraffa camelopardalis (Giraffe)

Taxonomic identifier

9894 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Giraffidae › Giraffa

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	Ribonuclease pancreatic		PRO_0000057198
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	119	1	Proton donor By similarity
	Binding site	7	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	85	1	Substrate By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Ref.2
	Disulfide bond	26 ↔ 84		By similarity
	Disulfide bond	40 ↔ 95		By similarity
	Disulfide bond	58 ↔ 110		By similarity
	Disulfide bond	65 ↔ 72		By similarity
Natural variations
	Natural variant	76	1	Y → N
Experimental info
	Sequence conflict	28	1	E → Q Ref.1
	Sequence conflict	28	1	E → Q Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00662-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: CE97EBDC792612DA	FASTA	124	13,704
	10 20 30 40 50 60 KESAAAKFER QHIDSSTSSV SSSNYCNEMM TSRNLTQDRC KPVNTFVHES LADVQAVCSQ 70 80 90 100 110 120 KNVACKNGQT NCYQSYSAMS ITDCRETGNS KYPNCAYQTT QAEKHIIVAC EGNPYVPVHY DASV

References

[1]	Gaastra W. Thesis (1975), University of Groningen, Netherlands Cited for: PROTEIN SEQUENCE.
[2]	"The primary structure of giraffe pancreatic ribonuclease." Gaastra W., Groen G., Welling G.W., Beintema J.J. FEBS Lett. 41:227-232(1974) [PubMed: 4855009] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Molecular evolution of genes encoding ribonucleases in ruminant species." Confalone E., Beintema J.J., Sasso M.P., Carsana A., Palmieri M., Vento M.T., Furia A. J. Mol. Evol. 41:850-858(1995) [PubMed: 8587129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequences related to the ox pancreatic ribonuclease coding region in the genomic DNA of mammalian species." Breukelman H.J., Beintema J.J., Confalone E., Costanzo C., Sasso M.P., Carsana A., Palmieri M., Furia A. J. Mol. Evol. 37:29-35(1993) [PubMed: 8360916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-114.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S81739 Genomic DNA. Translation: AAB36133.1. S65127 Genomic DNA. Translation: AAB27932.1.
PIR	NRGF. A94452.
3D structure databases
HSSP	HSSP built from PDB template 1SSB based on UniProtKB P00656.
SMR	P00662. Positions 1-124.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00662.
Enzyme and pathway databases
BRENDA	3.1.27.5. 289558.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_GIRCA

Accession

Primary (citable) accession number: P00662
Secondary accession number(s): Q29534, Q29541

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)