Reviewed, UniProtKB/Swiss-Prot P00660 (RNAS1_CONTA)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Connochaetes taurinus (Brindled gnu)

Taxonomic identifier

9927 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Alcelaphinae › Connochaetes

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	Ribonuclease pancreatic		PRO_0000057193
Regions
	Region	41 – 45	5	Substrate binding
Sites
	Active site	12	1	Proton acceptor
	Active site	119	1	Proton donor
	Binding site	7	1	Substrate
	Binding site	10	1	Substrate
	Binding site	66	1	Substrate
	Binding site	85	1	Substrate
Amino acid modifications
	Disulfide bond	26 ↔ 84
	Disulfide bond	40 ↔ 95
	Disulfide bond	58 ↔ 110
	Disulfide bond	65 ↔ 72

Sequences

	Sequence		Length	Mass (Da)
	P00660-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 3564BE9D9D871195	FASTA	124	13,686
	10 20 30 40 50 60 KESAAAKFER QHMDSSTSSA SSSNYCNQMM KSRNLTQDRC KPVNTFVHEP LADVQAVCSQ 70 80 90 100 110 120 KNVACKNGQT NCYQSYSTMS ITDCRETGSS KYPNCAYKAT QAKKHIIVAC EGNPYVPVHF DASV

References

[1]	"The amino acid sequence of gnu pancreatic ribonuclease." Groen G., Welling G.W., Beintema J.J. FEBS Lett. 60:300-304(1975) [PubMed: 1227969] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.

Cross-references

Sequence databases
PIR	NRGN. A00808.
3D structure databases
HSSP	HSSP built from PDB template 1SRN based on UniProtKB P00656.
SMR	P00660. Positions 1-124.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00660.
Enzyme and pathway databases
BRENDA	3.1.27.5. 293933.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_CONTA

Accession

Primary (citable) accession number: P00660

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)