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Protein

Ribonuclease U2

Gene

RNU2

Organism
Ustilago sphaerogena (Smut fungus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in A-P or G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Calcium 11 Publication1
Metal bindingi29Calcium 21 Publication1
Metal bindingi30Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi31Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi32Calcium 11 Publication1
Metal bindingi32Calcium 21 Publication1
Metal bindingi37Calcium 11 Publication1
Metal bindingi39Calcium 2; via carbonyl oxygen1 Publication1
Active sitei411 Publication1
Active sitei62Proton acceptor1 Publication1
Sitei62Methylation inactivates enzyme1 Publication1
Binding sitei85Substrate1
Active sitei101Proton donor1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.27.4. 6588.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease U2 (EC:3.1.27.4)
Short name:
RNase U2
Gene namesi
Name:RNU2
OrganismiUstilago sphaerogena (Smut fungus)
Taxonomic identifieri5271 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373791 – 114Ribonuclease U2Add BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi1 ↔ 541 Publication
Disulfide bondi9 ↔ 1131 Publication
Disulfide bondi55 ↔ 961 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1114
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi12 – 15Combined sources4
Helixi16 – 31Combined sources16
Helixi36 – 38Combined sources3
Beta strandi41 – 43Combined sources3
Helixi47 – 49Combined sources3
Beta strandi60 – 64Combined sources5
Beta strandi75 – 77Combined sources3
Beta strandi83 – 89Combined sources7
Turni90 – 92Combined sources3
Beta strandi95 – 101Combined sources7
Beta strandi105 – 109Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RTUX-ray1.80A1-114[»]
3AGNX-ray0.96A1-114[»]
3AGOX-ray0.99A1-114[»]
3AHSX-ray1.32A/B/C1-114[»]
3AHWX-ray1.03A1-114[»]
ProteinModelPortaliP00654.
SMRiP00654.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00654.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 49Substrate bindingAdd BLAST11
Regioni108 – 110Substrate binding3

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P00654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
CDIPQSTNCG GNVYSNDDIN TAIQGALDDV ANGDRPDNYP HQYYDEASED
60 70 80 90 100
ITLCCGSGPW SEFPLVYNGP YYSSRDNYVS PGPDRVIYQT NTGEFCATVT
110
HTGAASYDGF TQCS
Length:114
Mass (Da):12,387
Last modified:December 15, 1998 - v4
Checksum:i96D32CB2E23AAB98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004827 Genomic DNA. Translation: CAC04098.1.
PIRiPC4081. NRUSU2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004827 Genomic DNA. Translation: CAC04098.1.
PIRiPC4081. NRUSU2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RTUX-ray1.80A1-114[»]
3AGNX-ray0.96A1-114[»]
3AGOX-ray0.99A1-114[»]
3AHSX-ray1.32A/B/C1-114[»]
3AHWX-ray1.03A1-114[»]
ProteinModelPortaliP00654.
SMRiP00654.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.27.4. 6588.

Miscellaneous databases

EvolutionaryTraceiP00654.
PROiP00654.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNU2_USTSP
AccessioniPrimary (citable) accession number: P00654
Secondary accession number(s): Q9HGK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 94 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

After treatment by base Asn-32 and Asp-45 partially isomerise by succinimide rearrangement to form iosaspartyl peptides.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.