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P00653

- RNMS_ASPPH

UniProt

P00653 - RNMS_ASPPH

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Protein
Guanyl-specific ribonuclease Ms
Gene
N/A
Organism
Aspergillus phoenicis (Aspergillus saitoi)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391
Active sitei57 – 571Proton acceptor
Active sitei91 – 911Proton donor

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: InterPro
  3. ribonuclease T1 activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease Ms (EC:3.1.27.3)
Short name:
RNase Ms
OrganismiAspergillus phoenicis (Aspergillus saitoi)
Taxonomic identifieri5063 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 105105Guanyl-specific ribonuclease Ms
PRO_0000137370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 11
Disulfide bondi7 ↔ 102

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Beta strandi10 – 123
Helixi14 – 3017
Beta strandi37 – 415
Beta strandi55 – 606
Beta strandi74 – 807
Beta strandi85 – 917
Beta strandi94 – 974

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RDSX-ray1.80A1-105[»]
1RMSX-ray1.90A1-105[»]
ProteinModelPortaliP00653.
SMRiP00653. Positions 1-105.

Miscellaneous databases

EvolutionaryTraceiP00653.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P00653-1 [UniParc]FASTAAdd to Basket

« Hide

ESCEYTCGST CYWSSDVSAA KAKGYSLYES GDTIDDYPHG YHDYEGFDFP    50
VSGTYYEYPI MSDYDVYTGG SPGADRVIFN GDDELAGVIT HTGASGDDFV 100
ACSSS 105
Length:105
Mass (Da):11,332
Last modified:August 1, 1991 - v2
Checksum:i94CF56D109242EFD
GO

Sequence databases

PIRiA00800. NRASTP.

Cross-referencesi

Sequence databases

PIRi A00800. NRASTP.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RDS X-ray 1.80 A 1-105 [» ]
1RMS X-ray 1.90 A 1-105 [» ]
ProteinModelPortali P00653.
SMRi P00653. Positions 1-105.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00653.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Primary structure of a minor ribonuclease from Aspergillus saitoi."
    Watanabe H., Ohgi K., Irie M.
    J. Biochem. 91:1495-1509(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Three-dimensional structure of ribonuclease Ms*3'-guanylic acid complex at 2.5-A resolution."
    Nonaka T., Mitsui Y., Irie M., Nakamura K.T.
    FEBS Lett. 283:207-209(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SEQUENCE REVISION TO 52-54; 80 AND 95.

Entry informationi

Entry nameiRNMS_ASPPH
AccessioniPrimary (citable) accession number: P00653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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