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P00653

- RNMS_ASPPH

UniProt

P00653 - RNMS_ASPPH

Protein

Guanyl-specific ribonuclease Ms

Gene
N/A
Organism
Aspergillus phoenicis (Aspergillus saitoi)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei39 – 391
    Active sitei57 – 571Proton acceptor
    Active sitei91 – 911Proton donor

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. ribonuclease T1 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease Ms (EC:3.1.27.3)
    Short name:
    RNase Ms
    OrganismiAspergillus phoenicis (Aspergillus saitoi)
    Taxonomic identifieri5063 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 105105Guanyl-specific ribonuclease MsPRO_0000137370Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi3 ↔ 11
    Disulfide bondi7 ↔ 102

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi10 – 123
    Helixi14 – 3017
    Beta strandi37 – 415
    Beta strandi55 – 606
    Beta strandi74 – 807
    Beta strandi85 – 917
    Beta strandi94 – 974

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RDSX-ray1.80A1-105[»]
    1RMSX-ray1.90A1-105[»]
    ProteinModelPortaliP00653.
    SMRiP00653. Positions 1-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00653.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00653-1 [UniParc]FASTAAdd to Basket

    « Hide

    ESCEYTCGST CYWSSDVSAA KAKGYSLYES GDTIDDYPHG YHDYEGFDFP    50
    VSGTYYEYPI MSDYDVYTGG SPGADRVIFN GDDELAGVIT HTGASGDDFV 100
    ACSSS 105
    Length:105
    Mass (Da):11,332
    Last modified:August 1, 1991 - v2
    Checksum:i94CF56D109242EFD
    GO

    Sequence databases

    PIRiA00800. NRASTP.

    Cross-referencesi

    Sequence databases

    PIRi A00800. NRASTP.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RDS X-ray 1.80 A 1-105 [» ]
    1RMS X-ray 1.90 A 1-105 [» ]
    ProteinModelPortali P00653.
    SMRi P00653. Positions 1-105.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P00653.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a minor ribonuclease from Aspergillus saitoi."
      Watanabe H., Ohgi K., Irie M.
      J. Biochem. 91:1495-1509(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Three-dimensional structure of ribonuclease Ms*3'-guanylic acid complex at 2.5-A resolution."
      Nonaka T., Mitsui Y., Irie M., Nakamura K.T.
      FEBS Lett. 283:207-209(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SEQUENCE REVISION TO 52-54; 80 AND 95.

    Entry informationi

    Entry nameiRNMS_ASPPH
    AccessioniPrimary (citable) accession number: P00653
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3