Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P00653 (RNMS_ASPPH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease Ms

Short name=RNase Ms
EC=3.1.27.3
OrganismAspergillus phoenicis (Aspergillus saitoi)
Taxonomic identifier5063 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length105 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 105105Guanyl-specific ribonuclease Ms
PRO_0000137370

Sites

Active site391
Active site571Proton acceptor
Active site911Proton donor

Amino acid modifications

Disulfide bond3 ↔ 11
Disulfide bond7 ↔ 102

Secondary structure

................. 105
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00653 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: 94CF56D109242EFD

FASTA10511,332
        10         20         30         40         50         60 
ESCEYTCGST CYWSSDVSAA KAKGYSLYES GDTIDDYPHG YHDYEGFDFP VSGTYYEYPI 

        70         80         90        100 
MSDYDVYTGG SPGADRVIFN GDDELAGVIT HTGASGDDFV ACSSS 

« Hide

References

[1]"Primary structure of a minor ribonuclease from Aspergillus saitoi."
Watanabe H., Ohgi K., Irie M.
J. Biochem. 91:1495-1509(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"Three-dimensional structure of ribonuclease Ms*3'-guanylic acid complex at 2.5-A resolution."
Nonaka T., Mitsui Y., Irie M., Nakamura K.T.
FEBS Lett. 283:207-209(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SEQUENCE REVISION TO 52-54; 80 AND 95.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRNRASTP. A00800.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RDSX-ray1.80A1-105[»]
1RMSX-ray1.90A1-105[»]
ProteinModelPortalP00653.
SMRP00653. Positions 1-105.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP00653.

Entry information

Entry nameRNMS_ASPPH
AccessionPrimary (citable) accession number: P00653
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references