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Protein

Guanyl-specific ribonuclease C2

Gene

ACLA_055300

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66By similarity1
Active sitei84Proton acceptorBy similarity1
Active sitei118Proton donorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease C2 (EC:3.1.27.3)
Short name:
RNase C-2
Gene namesi
ORF Names:ACLA_055300
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_055300.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000013736927 – 132Guanyl-specific ribonuclease C2Add BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 36By similarity
Disulfide bondi32 ↔ 129By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00652.
SMRiP00652.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217295.
OrthoDBiEOG092C58FH.

Family and domain databases

InterProiView protein in InterPro
IPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
PfamiView protein in Pfam
PF00545. Ribonuclease. 1 hit.
PIRSFiPIRSF037430. RNase_U2. 1 hit.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYNKLITIA ALLVPALAAP QGLDVRDCDY TCGSHCYSAS AVSDAQSAGY
60 70 80 90 100
QLYSAGQSVG RSRYPHQYRN YEGFNFPVSG NYYEWPILSS GSTYNGGSPG
110 120 130
ADRVVFNDND ELAGLITHTG ASGNGFVACS GW
Length:132
Mass (Da):14,064
Last modified:April 3, 2007 - v2
Checksum:i3E2B1FEEFE0DBF84
GO

Sequence cautioni

The sequence EAW13482 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53Y → E AA sequence (Ref. 2) Curated1
Sequence conflicti98S → G AA sequence (Ref. 2) Curated1
Sequence conflicti124N → D AA sequence (Ref. 2) Curated1
Sequence conflicti130 – 132SGW → Y AA sequence (Ref. 2) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027048 Genomic DNA. Translation: EAW13482.1. Sequence problems.
PIRiA00799. NRASTC.
RefSeqiXP_001274908.1. XM_001274907.1.

Genome annotation databases

GeneIDi4707141.
KEGGiact:ACLA_055300.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRNC2_ASPCL
AccessioniPrimary (citable) accession number: P00652
Secondary accession number(s): A1C9F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 3, 2007
Last modified: June 7, 2017
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families