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P00652

- RNC2_ASPCL

UniProt

P00652 - RNC2_ASPCL

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Protein

Guanyl-specific ribonuclease C2

Gene

ACLA_055300

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661By similarity
Active sitei84 – 841Proton acceptorBy similarity
Active sitei118 – 1181Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. ribonuclease T1 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease C2 (EC:3.1.27.3)
Short name:
RNase C-2
Gene namesi
ORF Names:ACLA_055300
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006701: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 132106Guanyl-specific ribonuclease C2PRO_0000137369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 36By similarity
Disulfide bondi32 ↔ 129By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00652.
SMRiP00652. Positions 28-130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG114265.
HOGENOMiHOG000217295.
OrthoDBiEOG7XSTSF.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00652-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLYNKLITIA ALLVPALAAP QGLDVRDCDY TCGSHCYSAS AVSDAQSAGY
60 70 80 90 100
QLYSAGQSVG RSRYPHQYRN YEGFNFPVSG NYYEWPILSS GSTYNGGSPG
110 120 130
ADRVVFNDND ELAGLITHTG ASGNGFVACS GW
Length:132
Mass (Da):14,064
Last modified:April 3, 2007 - v2
Checksum:i3E2B1FEEFE0DBF84
GO

Sequence cautioni

The sequence EAW13482.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531Y → E AA sequence 1 PublicationCurated
Sequence conflicti98 – 981S → G AA sequence 1 PublicationCurated
Sequence conflicti124 – 1241N → D AA sequence 1 PublicationCurated
Sequence conflicti130 – 1323SGW → Y AA sequence 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027048 Genomic DNA. Translation: EAW13482.1. Sequence problems.
PIRiA00799. NRASTC.
RefSeqiXP_001274908.1. XM_001274907.1.

Genome annotation databases

GeneIDi4707141.
KEGGiact:ACLA_055300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027048 Genomic DNA. Translation: EAW13482.1 . Sequence problems.
PIRi A00799. NRASTC.
RefSeqi XP_001274908.1. XM_001274907.1.

3D structure databases

ProteinModelPortali P00652.
SMRi P00652. Positions 28-130.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4707141.
KEGGi act:ACLA_055300.

Phylogenomic databases

eggNOGi NOG114265.
HOGENOMi HOG000217295.
OrthoDBi EOG7XSTSF.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
  2. "The complete amino acid sequence of ribonuclease C-2 from Aspergillus clavatus."
    Bezborodova S.I., Khodova O.M., Stepanov V.M.
    FEBS Lett. 159:256-258(1983)
    Cited for: PROTEIN SEQUENCE OF 27-130.

Entry informationi

Entry nameiRNC2_ASPCL
AccessioniPrimary (citable) accession number: P00652
Secondary accession number(s): A1C9F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3