Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanyl-specific ribonuclease C2

Gene

ACLA_055300

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661By similarity
Active sitei84 – 841Proton acceptorBy similarity
Active sitei118 – 1181Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease C2 (EC:3.1.27.3)
Short name:
RNase C-2
Gene namesi
ORF Names:ACLA_055300
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_055300.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 132106Guanyl-specific ribonuclease C2PRO_0000137369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 36By similarity
Disulfide bondi32 ↔ 129By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00652.
SMRiP00652. Positions 28-130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217295.
OrthoDBiEOG7XSTSF.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYNKLITIA ALLVPALAAP QGLDVRDCDY TCGSHCYSAS AVSDAQSAGY
60 70 80 90 100
QLYSAGQSVG RSRYPHQYRN YEGFNFPVSG NYYEWPILSS GSTYNGGSPG
110 120 130
ADRVVFNDND ELAGLITHTG ASGNGFVACS GW
Length:132
Mass (Da):14,064
Last modified:April 3, 2007 - v2
Checksum:i3E2B1FEEFE0DBF84
GO

Sequence cautioni

The sequence EAW13482.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531Y → E AA sequence (Ref. 2) Curated
Sequence conflicti98 – 981S → G AA sequence (Ref. 2) Curated
Sequence conflicti124 – 1241N → D AA sequence (Ref. 2) Curated
Sequence conflicti130 – 1323SGW → Y AA sequence (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027048 Genomic DNA. Translation: EAW13482.1. Sequence problems.
PIRiA00799. NRASTC.
RefSeqiXP_001274908.1. XM_001274907.1.

Genome annotation databases

GeneIDi4707141.
KEGGiact:ACLA_055300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027048 Genomic DNA. Translation: EAW13482.1. Sequence problems.
PIRiA00799. NRASTC.
RefSeqiXP_001274908.1. XM_001274907.1.

3D structure databases

ProteinModelPortaliP00652.
SMRiP00652. Positions 28-130.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4707141.
KEGGiact:ACLA_055300.

Organism-specific databases

EuPathDBiFungiDB:ACLA_055300.

Phylogenomic databases

HOGENOMiHOG000217295.
OrthoDBiEOG7XSTSF.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
  2. "The complete amino acid sequence of ribonuclease C-2 from Aspergillus clavatus."
    Bezborodova S.I., Khodova O.M., Stepanov V.M.
    FEBS Lett. 159:256-258(1983)
    Cited for: PROTEIN SEQUENCE OF 27-130.

Entry informationi

Entry nameiRNC2_ASPCL
AccessioniPrimary (citable) accession number: P00652
Secondary accession number(s): A1C9F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 3, 2007
Last modified: December 9, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.