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P00652

- RNC2_ASPCL

UniProt

P00652 - RNC2_ASPCL

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Protein
Guanyl-specific ribonuclease C2
Gene
ACLA_055300
Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661 By similarity
Active sitei84 – 841Proton acceptor By similarity
Active sitei118 – 1181Proton donor By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: InterPro
  3. ribonuclease T1 activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease C2 (EC:3.1.27.3)
    Short name:
    RNase C-2
    Gene namesi
    ORF Names:ACLA_055300
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 Publication
    Add
    BLAST
    Chaini27 – 132106Guanyl-specific ribonuclease C2
    PRO_0000137369Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 36 By similarity
    Disulfide bondi32 ↔ 129 By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP00652.
    SMRiP00652. Positions 28-130.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG114265.
    HOGENOMiHOG000217295.
    OrthoDBiEOG7XSTSF.

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00652-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLYNKLITIA ALLVPALAAP QGLDVRDCDY TCGSHCYSAS AVSDAQSAGY    50
    QLYSAGQSVG RSRYPHQYRN YEGFNFPVSG NYYEWPILSS GSTYNGGSPG 100
    ADRVVFNDND ELAGLITHTG ASGNGFVACS GW 132
    Length:132
    Mass (Da):14,064
    Last modified:April 3, 2007 - v2
    Checksum:i3E2B1FEEFE0DBF84
    GO

    Sequence cautioni

    The sequence EAW13482.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531Y → E AA sequence 1 Publication
    Sequence conflicti98 – 981S → G AA sequence 1 Publication
    Sequence conflicti124 – 1241N → D AA sequence 1 Publication
    Sequence conflicti130 – 1323SGW → Y AA sequence 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027048 Genomic DNA. Translation: EAW13482.1. Sequence problems.
    PIRiA00799. NRASTC.
    RefSeqiXP_001274908.1. XM_001274907.1.

    Genome annotation databases

    GeneIDi4707141.
    KEGGiact:ACLA_055300.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027048 Genomic DNA. Translation: EAW13482.1 . Sequence problems.
    PIRi A00799. NRASTC.
    RefSeqi XP_001274908.1. XM_001274907.1.

    3D structure databases

    ProteinModelPortali P00652.
    SMRi P00652. Positions 28-130.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4707141.
    KEGGi act:ACLA_055300.

    Phylogenomic databases

    eggNOGi NOG114265.
    HOGENOMi HOG000217295.
    OrthoDBi EOG7XSTSF.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.
    2. "The complete amino acid sequence of ribonuclease C-2 from Aspergillus clavatus."
      Bezborodova S.I., Khodova O.M., Stepanov V.M.
      FEBS Lett. 159:256-258(1983)
      Cited for: PROTEIN SEQUENCE OF 27-130.

    Entry informationi

    Entry nameiRNC2_ASPCL
    AccessioniPrimary (citable) accession number: P00652
    Secondary accession number(s): A1C9F8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 3, 2007
    Last modified: April 16, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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