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P00650

- RNST_SACER

UniProt

P00650 - RNST_SACER

Protein

Guanyl-specific ribonuclease St

Gene
N/A
Organism
Saccharopolyspora erythraea (Streptomyces erythraeus)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611Proton acceptorBy similarity
    Active sitei91 – 911Proton donorBy similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. ribonuclease T1 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease St (EC:3.1.27.3)
    Short name:
    RNase St
    OrganismiSaccharopolyspora erythraea (Streptomyces erythraeus)
    Taxonomic identifieri1836 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 101101Guanyl-specific ribonuclease StPRO_0000137368Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi4 ↔ 541 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP00650.
    SMRiP00650. Positions 6-67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00650-1 [UniParc]FASTAAdd to Basket

    « Hide

    EAPCGDTSGF EQVRLADLPP EATDTYELIE KGGPYPYPED GTVFENREGI    50
    LPDCAEGYYH EYTVKTPSGD DRGARRFVVG DGGEYFYTED HYESFRLTIV 100
    N 101
    Length:101
    Mass (Da):11,354
    Last modified:July 21, 1986 - v1
    Checksum:iE739ADB75A61A9FB
    GO

    Sequence databases

    PIRiA91429. NRSMTE.

    Cross-referencesi

    Sequence databases

    PIRi A91429. NRSMTE.

    3D structure databases

    ProteinModelPortali P00650.
    SMRi P00650. Positions 6-67.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequence of ribonuclease St."
      Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
      FEBS Lett. 64:122-125(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
      Submitted (JUN-1977) to the PIR data bank
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiRNST_SACER
    AccessioniPrimary (citable) accession number: P00650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3