Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P00650 (RNST_SACER) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease St

Short name=RNase St
EC=3.1.27.3
OrganismSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifier1836 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length101 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 101101Guanyl-specific ribonuclease St
PRO_0000137368

Sites

Active site611Proton acceptor By similarity
Active site911Proton donor By similarity

Amino acid modifications

Disulfide bond4 ↔ 54 Ref.1

Sequences

Sequence LengthMass (Da)Tools
P00650 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: E739ADB75A61A9FB

FASTA10111,354
        10         20         30         40         50         60 
EAPCGDTSGF EQVRLADLPP EATDTYELIE KGGPYPYPED GTVFENREGI LPDCAEGYYH 

        70         80         90        100 
EYTVKTPSGD DRGARRFVVG DGGEYFYTED HYESFRLTIV N 

« Hide

References

[1]"The amino acid sequence of ribonuclease St."
Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
FEBS Lett. 64:122-125(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
Submitted (JUN-1977) to the PIR data bank
Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases

PIRNRSMTE. A91429.

3D structure databases

ProteinModelPortalP00650.
SMRP00650. Positions 6-67.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNST_SACER
AccessionPrimary (citable) accession number: P00650
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families