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P00650

- RNST_SACER

UniProt

P00650 - RNST_SACER

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Protein
Guanyl-specific ribonuclease St
Gene
N/A
Organism
Saccharopolyspora erythraea (Streptomyces erythraeus)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611Proton acceptor By similarity
Active sitei91 – 911Proton donor By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: InterPro
  3. ribonuclease T1 activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease St (EC:3.1.27.3)
Short name:
RNase St
OrganismiSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifieri1836 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 101101Guanyl-specific ribonuclease St
PRO_0000137368Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 ↔ 541 Publication

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00650.
SMRiP00650. Positions 6-67.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P00650-1 [UniParc]FASTAAdd to Basket

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EAPCGDTSGF EQVRLADLPP EATDTYELIE KGGPYPYPED GTVFENREGI    50
LPDCAEGYYH EYTVKTPSGD DRGARRFVVG DGGEYFYTED HYESFRLTIV 100
N 101
Length:101
Mass (Da):11,354
Last modified:July 21, 1986 - v1
Checksum:iE739ADB75A61A9FB
GO

Sequence databases

PIRiA91429. NRSMTE.

Cross-referencesi

Sequence databases

PIRi A91429. NRSMTE.

3D structure databases

ProteinModelPortali P00650.
SMRi P00650. Positions 6-67.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The amino acid sequence of ribonuclease St."
    Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
    FEBS Lett. 64:122-125(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. Yoshida N., Sasaki A., Rashid M.A., Otsuka H.
    Submitted (JUN-1977) to the PIR data bank
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiRNST_SACER
AccessioniPrimary (citable) accession number: P00650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3