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P00649

- RN_BACIN

UniProt

P00649 - RN_BACIN

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Protein

Ribonuclease

Gene
N/A
Organism
Bacillus intermedius
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This is a purine-specific ribonuclease.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251Proton acceptor
Active sitei154 – 1541Proton donor

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease (EC:3.1.27.-)
Alternative name(s):
Binase
RNase Bi
OrganismiBacillus intermedius
Taxonomic identifieri1400 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Propeptidei30 – 53241 PublicationPRO_0000030824Add
BLAST
Chaini54 – 162109RibonucleasePRO_0000030825Add
BLAST

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi59 – 6911
Helixi79 – 846
Helixi89 – 913
Helixi94 – 974
Beta strandi102 – 1087
Beta strandi112 – 1154
Beta strandi118 – 1203
Beta strandi123 – 1275
Beta strandi132 – 1343
Beta strandi139 – 1435
Beta strandi148 – 1536
Beta strandi159 – 1624

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BUJNMR-A54-162[»]
1GOUX-ray1.65A/B54-162[»]
1GOVX-ray2.00A/B54-162[»]
1GOYX-ray2.00A/B54-162[»]
2RBIX-ray2.20A/B54-162[»]
4HAAX-ray1.90A/B/C/D54-162[»]
ProteinModelPortaliP00649.
SMRiP00649. Positions 54-162.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00649.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR001887. Barnase.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF001013. Barnase. 1 hit.
PRINTSiPR00117. BARNASE.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00649-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKISSVFTM FALIAAILFS GFIPQQAYAE TPLTQTATNE TATIQLTSDV
60 70 80 90 100
HTLAVINTFD GVADYLIRYK RLPDNYITKS QASALGWVAS KGNLAEVAPG
110 120 130 140 150
KSIGGDVFSN REGRLPSASG RTWREADINY VSGFRNADRL VYSSDWLIYK
160
TTDHYATFTR IR
Length:162
Mass (Da):17,956
Last modified:May 1, 1992 - v3
Checksum:iCD6740EDFBCF5295
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 752DN → ND AA sequence (PubMed:761620)Curated
Sequence conflicti93 – 931N → D AA sequence (PubMed:761620)Curated
Sequence conflicti119 – 1202SG → GS AA sequence (PubMed:761620)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53697 Genomic DNA. Translation: CAA37735.1.
PIRiA00796. NRBSI.
S22653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53697 Genomic DNA. Translation: CAA37735.1 .
PIRi A00796. NRBSI.
S22653.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BUJ NMR - A 54-162 [» ]
1GOU X-ray 1.65 A/B 54-162 [» ]
1GOV X-ray 2.00 A/B 54-162 [» ]
1GOY X-ray 2.00 A/B 54-162 [» ]
2RBI X-ray 2.20 A/B 54-162 [» ]
4HAA X-ray 1.90 A/B/C/D 54-162 [» ]
ProteinModelPortali P00649.
SMRi P00649. Positions 54-162.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00649.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR001887. Barnase.
IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
PIRSFi PIRSF001013. Barnase. 1 hit.
PRINTSi PR00117. BARNASE.
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of the gene encoding RNase binase from Bacillus intermedius 7P."
    Schulga A.A., Nurkiyanova K.M., Zakharyev V.M., Kirpichnikov M.P., Skryabin K.G.
    Nucleic Acids Res. 20:2375-2375(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 7P.
  2. "The cloning and determination of the nucleotide sequence of the RNase gene in Bacillus intermedius."
    Nurkiyanova K.M., Shulga A.A., Zakharyev V.M., Kirpichnikov M.P., Skryabin K.G., Baev A.A.
    Dokl. Akad. Nauk SSSR 309:1476-1479(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 7P.
  3. "Primary structure of ribonuclease from Bacillus intermedius 7P."
    Aphanasenko G.A., Dudkin S.M., Kaminir L.B., Leshchinskaya I.B., Severin E.S.
    FEBS Lett. 97:77-80(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 54-162.
    Strain: 7P.
  4. "RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations."
    Okorokov A.L., Panov K.I., Offen W.A., Mukhortov V.G., Antson A.A., Karpeisky M.Y., Wilkinson A.J., Dodson G.G.
    Protein Eng. 10:273-278(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) AND MUTAGENESIS OF HIS-154.
  5. Cited for: STRUCTURE BY NMR.
  6. "The structure of substrate-free microbial ribonuclease binase and of its complexes with 3'GMP and sulfate ions."
    Polyakov K.M., Lebedev A.A., Okorokov A.L., Panov K.I., Schulga A.A., Pavlovsky A.G., Karpeisky M.Y., Dodson G.G.
    Acta Crystallogr. D 58:744-750(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 54-162.

Entry informationi

Entry nameiRN_BACIN
AccessioniPrimary (citable) accession number: P00649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3