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Protein

Ribonuclease

Gene
N/A
Organism
Bacillus intermedius
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This is a purine-specific ribonuclease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125Proton acceptor1
Active sitei154Proton donor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.3. 664.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease (EC:3.1.27.-)
Alternative name(s):
Binase
RNase Bi
OrganismiBacillus intermedius
Taxonomic identifieri1400 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000003082430 – 531 PublicationAdd BLAST24
ChainiPRO_000003082554 – 162RibonucleaseAdd BLAST109

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi59 – 69Combined sources11
Helixi79 – 84Combined sources6
Helixi89 – 91Combined sources3
Helixi94 – 97Combined sources4
Beta strandi102 – 108Combined sources7
Beta strandi112 – 115Combined sources4
Beta strandi118 – 120Combined sources3
Beta strandi123 – 127Combined sources5
Beta strandi132 – 134Combined sources3
Beta strandi139 – 143Combined sources5
Beta strandi148 – 153Combined sources6
Beta strandi159 – 162Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUJNMR-A54-162[»]
1GOUX-ray1.65A/B54-162[»]
1GOVX-ray2.00A/B54-162[»]
1GOYX-ray2.00A/B54-162[»]
2RBIX-ray2.20A/B54-162[»]
4HAAX-ray1.90A/B/C/D54-162[»]
ProteinModelPortaliP00649.
SMRiP00649.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00649.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR001887. Barnase.
IPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF001013. Barnase. 1 hit.
PRINTSiPR00117. BARNASE.
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKISSVFTM FALIAAILFS GFIPQQAYAE TPLTQTATNE TATIQLTSDV
60 70 80 90 100
HTLAVINTFD GVADYLIRYK RLPDNYITKS QASALGWVAS KGNLAEVAPG
110 120 130 140 150
KSIGGDVFSN REGRLPSASG RTWREADINY VSGFRNADRL VYSSDWLIYK
160
TTDHYATFTR IR
Length:162
Mass (Da):17,956
Last modified:May 1, 1992 - v3
Checksum:iCD6740EDFBCF5295
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74 – 75DN → ND AA sequence (PubMed:761620).Curated2
Sequence conflicti93N → D AA sequence (PubMed:761620).Curated1
Sequence conflicti119 – 120SG → GS AA sequence (PubMed:761620).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53697 Genomic DNA. Translation: CAA37735.1.
PIRiA00796. NRBSI.
S22653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53697 Genomic DNA. Translation: CAA37735.1.
PIRiA00796. NRBSI.
S22653.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BUJNMR-A54-162[»]
1GOUX-ray1.65A/B54-162[»]
1GOVX-ray2.00A/B54-162[»]
1GOYX-ray2.00A/B54-162[»]
2RBIX-ray2.20A/B54-162[»]
4HAAX-ray1.90A/B/C/D54-162[»]
ProteinModelPortaliP00649.
SMRiP00649.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.27.3. 664.

Miscellaneous databases

EvolutionaryTraceiP00649.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR001887. Barnase.
IPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
PIRSFiPIRSF001013. Barnase. 1 hit.
PRINTSiPR00117. BARNASE.
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRN_BACIN
AccessioniPrimary (citable) accession number: P00649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.