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P00649

- RN_BACIN

UniProt

P00649 - RN_BACIN

Protein

Ribonuclease

Gene
N/A
Organism
Bacillus intermedius
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This is a purine-specific ribonuclease.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei125 – 1251Proton acceptor
    Active sitei154 – 1541Proton donor

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease (EC:3.1.27.-)
    Alternative name(s):
    Binase
    RNase Bi
    OrganismiBacillus intermedius
    Taxonomic identifieri1400 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Propeptidei30 – 53241 PublicationPRO_0000030824Add
    BLAST
    Chaini54 – 162109RibonucleasePRO_0000030825Add
    BLAST

    Structurei

    Secondary structure

    1
    162
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi59 – 6911
    Helixi79 – 846
    Helixi89 – 913
    Helixi94 – 974
    Beta strandi102 – 1087
    Beta strandi112 – 1154
    Beta strandi118 – 1203
    Beta strandi123 – 1275
    Beta strandi132 – 1343
    Beta strandi139 – 1435
    Beta strandi148 – 1536
    Beta strandi159 – 1624

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BUJNMR-A54-162[»]
    1GOUX-ray1.65A/B54-162[»]
    1GOVX-ray2.00A/B54-162[»]
    1GOYX-ray2.00A/B54-162[»]
    2RBIX-ray2.20A/B54-162[»]
    4HAAX-ray1.90A/B/C/D54-162[»]
    ProteinModelPortaliP00649.
    SMRiP00649. Positions 54-162.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00649.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR001887. Barnase.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001013. Barnase. 1 hit.
    PRINTSiPR00117. BARNASE.
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00649-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKISSVFTM FALIAAILFS GFIPQQAYAE TPLTQTATNE TATIQLTSDV    50
    HTLAVINTFD GVADYLIRYK RLPDNYITKS QASALGWVAS KGNLAEVAPG 100
    KSIGGDVFSN REGRLPSASG RTWREADINY VSGFRNADRL VYSSDWLIYK 150
    TTDHYATFTR IR 162
    Length:162
    Mass (Da):17,956
    Last modified:May 1, 1992 - v3
    Checksum:iCD6740EDFBCF5295
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 752DN → ND AA sequence (PubMed:761620)Curated
    Sequence conflicti93 – 931N → D AA sequence (PubMed:761620)Curated
    Sequence conflicti119 – 1202SG → GS AA sequence (PubMed:761620)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53697 Genomic DNA. Translation: CAA37735.1.
    PIRiA00796. NRBSI.
    S22653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53697 Genomic DNA. Translation: CAA37735.1 .
    PIRi A00796. NRBSI.
    S22653.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BUJ NMR - A 54-162 [» ]
    1GOU X-ray 1.65 A/B 54-162 [» ]
    1GOV X-ray 2.00 A/B 54-162 [» ]
    1GOY X-ray 2.00 A/B 54-162 [» ]
    2RBI X-ray 2.20 A/B 54-162 [» ]
    4HAA X-ray 1.90 A/B/C/D 54-162 [» ]
    ProteinModelPortali P00649.
    SMRi P00649. Positions 54-162.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P00649.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR001887. Barnase.
    IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001013. Barnase. 1 hit.
    PRINTSi PR00117. BARNASE.
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the gene encoding RNase binase from Bacillus intermedius 7P."
      Schulga A.A., Nurkiyanova K.M., Zakharyev V.M., Kirpichnikov M.P., Skryabin K.G.
      Nucleic Acids Res. 20:2375-2375(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 7P.
    2. "The cloning and determination of the nucleotide sequence of the RNase gene in Bacillus intermedius."
      Nurkiyanova K.M., Shulga A.A., Zakharyev V.M., Kirpichnikov M.P., Skryabin K.G., Baev A.A.
      Dokl. Akad. Nauk SSSR 309:1476-1479(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 7P.
    3. "Primary structure of ribonuclease from Bacillus intermedius 7P."
      Aphanasenko G.A., Dudkin S.M., Kaminir L.B., Leshchinskaya I.B., Severin E.S.
      FEBS Lett. 97:77-80(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 54-162.
      Strain: 7P.
    4. "RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations."
      Okorokov A.L., Panov K.I., Offen W.A., Mukhortov V.G., Antson A.A., Karpeisky M.Y., Wilkinson A.J., Dodson G.G.
      Protein Eng. 10:273-278(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) AND MUTAGENESIS OF HIS-154.
    5. Cited for: STRUCTURE BY NMR.
    6. "The structure of substrate-free microbial ribonuclease binase and of its complexes with 3'GMP and sulfate ions."
      Polyakov K.M., Lebedev A.A., Okorokov A.L., Panov K.I., Schulga A.A., Pavlovsky A.G., Karpeisky M.Y., Dodson G.G.
      Acta Crystallogr. D 58:744-750(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 54-162.

    Entry informationi

    Entry nameiRN_BACIN
    AccessioniPrimary (citable) accession number: P00649
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3