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Protein

Colicin-E3

Gene

ceaC

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E3 (EC:3.1.-.-)
Alternative name(s):
Colicin-E3 A chain
Ribonuclease
Gene namesi
Name:ceaC
Encoded oniPlasmid ColE3-CA380 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186771 – 551Colicin-E3Add BLAST551

Proteomic databases

PRIDEiP00646.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
immP029842EBI-1029919,EBI-1029912

Protein-protein interaction databases

DIPiDIP-545N.
IntActiP00646. 2 interactors.
MINTiMINT-145709.

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi93 – 96Combined sources4
Beta strandi102 – 108Combined sources7
Helixi114 – 123Combined sources10
Beta strandi128 – 130Combined sources3
Beta strandi132 – 139Combined sources8
Helixi141 – 143Combined sources3
Beta strandi147 – 150Combined sources4
Beta strandi154 – 160Combined sources7
Helixi161 – 164Combined sources4
Beta strandi165 – 167Combined sources3
Helixi169 – 171Combined sources3
Beta strandi177 – 182Combined sources6
Beta strandi184 – 192Combined sources9
Beta strandi194 – 202Combined sources9
Beta strandi204 – 210Combined sources7
Beta strandi211 – 214Combined sources4
Beta strandi220 – 222Combined sources3
Beta strandi230 – 237Combined sources8
Beta strandi239 – 241Combined sources3
Beta strandi248 – 250Combined sources3
Beta strandi253 – 255Combined sources3
Turni261 – 266Combined sources6
Beta strandi267 – 273Combined sources7
Helixi276 – 278Combined sources3
Beta strandi283 – 289Combined sources7
Helixi293 – 313Combined sources21
Helixi315 – 375Combined sources61
Helixi376 – 378Combined sources3
Beta strandi382 – 385Combined sources4
Helixi386 – 448Combined sources63
Helixi457 – 460Combined sources4
Turni461 – 463Combined sources3
Helixi470 – 472Combined sources3
Beta strandi475 – 477Combined sources3
Beta strandi480 – 482Combined sources3
Beta strandi489 – 492Combined sources4
Beta strandi497 – 500Combined sources4
Turni501 – 504Combined sources4
Beta strandi505 – 510Combined sources6
Turni511 – 514Combined sources4
Beta strandi515 – 520Combined sources6
Turni521 – 523Combined sources3
Beta strandi525 – 530Combined sources6
Turni532 – 534Combined sources3
Beta strandi537 – 539Combined sources3
Helixi548 – 550Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E44X-ray2.40B456-551[»]
1JCHX-ray3.02A/C1-551[»]
1UJWX-ray2.75B314-448[»]
2B5UX-ray2.30A/C1-551[»]
4UDMX-ray2.96B456-551[»]
4V5KX-ray3.20AY/CY455-551[»]
ProteinModelPortaliP00646.
SMRiP00646.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00646.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni455 – 551Ribosome inactivating activityAdd BLAST97
Regioni530 – 551Binding of immunity proteinAdd BLAST22

Sequence similaritiesi

Belongs to the cloacin colicin family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.10.380.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SUPFAMiSSF63840. SSF63840. 1 hit.
SSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

P00646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGIHWGGGS GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA
110 120 130 140 150
GGLAVSISAG ALSAAIADIM AALKGPFKFG LWGVALYGVL PSQIAKDDPN
160 170 180 190 200
MMSKIVTSLP ADDITESPVS SLPLDKATVN VNVRVVDDVK DERQNISVVS
210 220 230 240 250
GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA VQTLSPGVTN
260 270 280 290 300
NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ
310 320 330 340 350
DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV
360 370 380 390 400
YNSRKSELDA ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA
410 420 430 440 450
QTDVNNKQAA FDAAAKEKSD ADAALSSAME SRKKKEDKKR SAENNLNDEK
460 470 480 490 500
NKPRKGFKDY GHDYHPAPKT ENIKGLGDLK PGIPKTPKQN GGGKRKRWTG
510 520 530 540 550
DKGRKIYEWD SQHGELEGYR ASDGQHLGSF DPKTGNQLKG PDPKRNIKKY

L
Length:551
Mass (Da):57,963
Last modified:April 1, 1988 - v1
Checksum:iE444CE918D89ECD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01574 Genomic DNA. Translation: AAA88416.1.
X02397 Genomic DNA. Translation: CAA26241.1.
X01162 Genomic DNA. Translation: CAA25607.1.
PIRiS07269. NRECE3.
RefSeqiWP_000012964.1. NZ_LOFR01000008.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01574 Genomic DNA. Translation: AAA88416.1.
X02397 Genomic DNA. Translation: CAA26241.1.
X01162 Genomic DNA. Translation: CAA25607.1.
PIRiS07269. NRECE3.
RefSeqiWP_000012964.1. NZ_LOFR01000008.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E44X-ray2.40B456-551[»]
1JCHX-ray3.02A/C1-551[»]
1UJWX-ray2.75B314-448[»]
2B5UX-ray2.30A/C1-551[»]
4UDMX-ray2.96B456-551[»]
4V5KX-ray3.20AY/CY455-551[»]
ProteinModelPortaliP00646.
SMRiP00646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-545N.
IntActiP00646. 2 interactors.
MINTiMINT-145709.

Proteomic databases

PRIDEiP00646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00646.

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.10.380.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SUPFAMiSSF63840. SSF63840. 1 hit.
SSF69369. SSF69369. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCEA3_ECOLX
AccessioniPrimary (citable) accession number: P00646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.