Colicin-E3 - Escherichia coli

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P00646 (CEA3_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Colicin-E3
EC=3.1.-.-

Alternative name(s):
Colicin-E3 A chain
Ribonuclease

Gene names

Name:

ceaC

Encoded on

Plasmid ColE3-CA38

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sequence similarities

Belongs to the cloacin colicin family.

Ontologies

Keywords
Molecular function	Antibiotic Antimicrobial Bacteriocin Endonuclease Hydrolase Nuclease
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extrachromosomal circular DNA Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW ribosome binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
imm	P02984	2	EBI-1029919,EBI-1029912

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 551

551

Colicin-E3

PRO_0000218677

Regions

Region

455 – 551

Ribosome inactivating activity

Region

530 – 551

Binding of immunity protein

Secondary structure

551

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P00646 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: E444CE918D89ECD6
Show »

FASTA

551

57,963

        10         20         30         40         50         60 
MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS GSGIHWGGGS 

        70         80         90        100        110        120 
GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAG ALSAAIADIM 

       130        140        150        160        170        180 
AALKGPFKFG LWGVALYGVL PSQIAKDDPN MMSKIVTSLP ADDITESPVS SLPLDKATVN 

       190        200        210        220        230        240 
VNVRVVDDVK DERQNISVVS GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA 

       250        260        270        280        290        300 
VQTLSPGVTN NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ 

       310        320        330        340        350        360 
DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV YNSRKSELDA 

       370        380        390        400        410        420 
ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA QTDVNNKQAA FDAAAKEKSD 

       430        440        450        460        470        480 
ADAALSSAME SRKKKEDKKR SAENNLNDEK NKPRKGFKDY GHDYHPAPKT ENIKGLGDLK 

       490        500        510        520        530        540 
PGIPKTPKQN GGGKRKRWTG DKGRKIYEWD SQHGELEGYR ASDGQHLGSF DPKTGNQLKG 

       550 
PDPKRNIKKY L

« Hide

References

[1]	"Colicin E3 and its immunity genes." Masaki H., Ohta T. J. Mol. Biol. 182:217-227(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3." Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P. Nucleic Acids Res. 12:8733-8745(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 370-551.
[3]	"Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin." Mock M., Miyada C.G., Gunsalus R.P. Nucleic Acids Res. 11:3547-3557(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-551.
[4]	"A plasmid region encoding the active fragment and the inhibitor protein of colicin E3-CA38." Masaki H., Ohta T. FEBS Lett. 149:129-132(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 445-551.
[5]	"Amino acid sequence of an active fragment (T2A) of colicin E3." Suzuki K., Imahori K. J. Biochem. 84:1031-1039(1978) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 455-551.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01574 Genomic DNA. Translation: AAA88416.1.
X02397 Genomic DNA. Translation: CAA26241.1.
X01162 Genomic DNA. Translation: CAA25607.1.

PIR

NRECE3. S07269.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E44	X-ray	2.40	B	456-551	[»]
1JCH	X-ray	3.02	A/C	1-551	[»]
1UJW	X-ray	2.75	B	314-448	[»]
2B5U	X-ray	2.30	A/C	1-551	[»]
2XFZ	X-ray	3.20	Y	455-551	[»]
2XG1	X-ray	3.20	Y	455-551	[»]

ProteinModelPortal

P00646.

SMR

P00646. Positions 82-551.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-545N.

IntAct

P00646. 2 interactions.

MINT

MINT-145709.

Proteomic databases

PRIDE

P00646.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.287.620. 1 hit.
3.10.380.10. 1 hit.

InterPro

IPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]

Pfam

PF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
PF06958. Pyocin_S. 1 hit.
[Graphical view]

PRINTS

PR01295. CLOACIN.

SUPFAM

SSF63840. Colicin_E3_cat. 1 hit.
SSF69369. Pyoson/cloacin_translocation. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P00646.

Entry information

Entry name

CEA3_ECOLX

Accession

Primary (citable) accession number: P00646

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Family and domain databases

Other

Entry information

Relevant documents