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P00646 (CEA3_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Colicin-E3

EC=3.1.-.-
Alternative name(s):
Colicin-E3 A chain
Ribonuclease
Gene names
Name:ceaC
Encoded onPlasmid ColE3-CA38
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sequence similarities

Belongs to the cloacin colicin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

immP029842EBI-1029919,EBI-1029912

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Colicin-E3
PRO_0000218677

Regions

Region455 – 55197Ribosome inactivating activity
Region530 – 55122Binding of immunity protein

Secondary structure

................................................................................. 551
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00646 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: E444CE918D89ECD6

FASTA55157,963
        10         20         30         40         50         60 
MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS GSGIHWGGGS 

        70         80         90        100        110        120 
GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAG ALSAAIADIM 

       130        140        150        160        170        180 
AALKGPFKFG LWGVALYGVL PSQIAKDDPN MMSKIVTSLP ADDITESPVS SLPLDKATVN 

       190        200        210        220        230        240 
VNVRVVDDVK DERQNISVVS GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA 

       250        260        270        280        290        300 
VQTLSPGVTN NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ 

       310        320        330        340        350        360 
DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV YNSRKSELDA 

       370        380        390        400        410        420 
ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA QTDVNNKQAA FDAAAKEKSD 

       430        440        450        460        470        480 
ADAALSSAME SRKKKEDKKR SAENNLNDEK NKPRKGFKDY GHDYHPAPKT ENIKGLGDLK 

       490        500        510        520        530        540 
PGIPKTPKQN GGGKRKRWTG DKGRKIYEWD SQHGELEGYR ASDGQHLGSF DPKTGNQLKG 

       550 
PDPKRNIKKY L 

« Hide

References

[1]"Colicin E3 and its immunity genes."
Masaki H., Ohta T.
J. Mol. Biol. 182:217-227(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3."
Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P.
Nucleic Acids Res. 12:8733-8745(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 370-551.
[3]"Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin."
Mock M., Miyada C.G., Gunsalus R.P.
Nucleic Acids Res. 11:3547-3557(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-551.
[4]"A plasmid region encoding the active fragment and the inhibitor protein of colicin E3-CA38."
Masaki H., Ohta T.
FEBS Lett. 149:129-132(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 445-551.
[5]"Amino acid sequence of an active fragment (T2A) of colicin E3."
Suzuki K., Imahori K.
J. Biochem. 84:1031-1039(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 455-551.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01574 Genomic DNA. Translation: AAA88416.1.
X02397 Genomic DNA. Translation: CAA26241.1.
X01162 Genomic DNA. Translation: CAA25607.1.
PIRNRECE3. S07269.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E44X-ray2.40B456-551[»]
1JCHX-ray3.02A/C1-551[»]
1UJWX-ray2.75B314-448[»]
2B5UX-ray2.30A/C1-551[»]
2XFZX-ray3.20Y455-551[»]
2XG1X-ray3.20Y455-551[»]
ProteinModelPortalP00646.
SMRP00646. Positions 82-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-545N.
IntActP00646. 2 interactions.
MINTMINT-145709.

Proteomic databases

PRIDEP00646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.287.620. 1 hit.
3.10.380.10. 1 hit.
InterProIPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamPF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
PF06958. Pyocin_S. 1 hit.
[Graphical view]
PRINTSPR01295. CLOACIN.
SUPFAMSSF63840. SSF63840. 1 hit.
SSF69369. SSF69369. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP00646.

Entry information

Entry nameCEA3_ECOLX
AccessionPrimary (citable) accession number: P00646
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references