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P00646

- CEA3_ECOLX

UniProt

P00646 - CEA3_ECOLX

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Protein

Colicin-E3

Gene

ceaC

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. ribosome binding Source: InterPro
  3. RNA binding Source: InterPro

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E3 (EC:3.1.-.-)
Alternative name(s):
Colicin-E3 A chain
Ribonuclease
Gene namesi
Name:ceaC
Encoded oniPlasmid ColE3-CA380 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. extrachromosomal circular DNA Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Colicin-E3PRO_0000218677Add
BLAST

Proteomic databases

PRIDEiP00646.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
immP029842EBI-1029919,EBI-1029912

Protein-protein interaction databases

DIPiDIP-545N.
IntActiP00646. 2 interactions.
MINTiMINT-145709.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi93 – 964
Beta strandi102 – 1087
Helixi114 – 12310
Beta strandi128 – 1303
Beta strandi132 – 1398
Helixi141 – 1433
Beta strandi147 – 1504
Beta strandi154 – 1607
Helixi161 – 1644
Beta strandi165 – 1673
Helixi169 – 1713
Beta strandi177 – 1826
Beta strandi184 – 1929
Beta strandi194 – 2029
Beta strandi204 – 2107
Beta strandi211 – 2144
Beta strandi220 – 2223
Beta strandi230 – 2378
Beta strandi239 – 2413
Beta strandi248 – 2503
Beta strandi253 – 2553
Turni261 – 2666
Beta strandi267 – 2737
Helixi276 – 2783
Beta strandi283 – 2897
Helixi293 – 31321
Helixi315 – 37561
Helixi376 – 3783
Beta strandi382 – 3854
Helixi386 – 44863
Helixi457 – 4604
Turni461 – 4633
Helixi470 – 4723
Beta strandi475 – 4773
Beta strandi480 – 4823
Beta strandi489 – 4924
Beta strandi497 – 5004
Turni501 – 5044
Beta strandi505 – 5106
Turni511 – 5144
Beta strandi515 – 5206
Turni521 – 5233
Beta strandi525 – 5306
Turni532 – 5343
Beta strandi537 – 5393
Helixi548 – 5503

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E44X-ray2.40B456-551[»]
1JCHX-ray3.02A/C1-551[»]
1UJWX-ray2.75B314-448[»]
2B5UX-ray2.30A/C1-551[»]
2XFZX-ray3.20Y455-551[»]
2XG1X-ray3.20Y455-551[»]
ProteinModelPortaliP00646.
SMRiP00646. Positions 82-551.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00646.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni455 – 55197Ribosome inactivating activityAdd
BLAST
Regioni530 – 55122Binding of immunity proteinAdd
BLAST

Sequence similaritiesi

Belongs to the cloacin colicin family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.10.380.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
PF06958. Pyocin_S. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SUPFAMiSSF63840. SSF63840. 1 hit.
SSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

P00646-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGIHWGGGS GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA
110 120 130 140 150
GGLAVSISAG ALSAAIADIM AALKGPFKFG LWGVALYGVL PSQIAKDDPN
160 170 180 190 200
MMSKIVTSLP ADDITESPVS SLPLDKATVN VNVRVVDDVK DERQNISVVS
210 220 230 240 250
GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA VQTLSPGVTN
260 270 280 290 300
NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ
310 320 330 340 350
DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV
360 370 380 390 400
YNSRKSELDA ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA
410 420 430 440 450
QTDVNNKQAA FDAAAKEKSD ADAALSSAME SRKKKEDKKR SAENNLNDEK
460 470 480 490 500
NKPRKGFKDY GHDYHPAPKT ENIKGLGDLK PGIPKTPKQN GGGKRKRWTG
510 520 530 540 550
DKGRKIYEWD SQHGELEGYR ASDGQHLGSF DPKTGNQLKG PDPKRNIKKY

L
Length:551
Mass (Da):57,963
Last modified:April 1, 1988 - v1
Checksum:iE444CE918D89ECD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01574 Genomic DNA. Translation: AAA88416.1.
X02397 Genomic DNA. Translation: CAA26241.1.
X01162 Genomic DNA. Translation: CAA25607.1.
PIRiS07269. NRECE3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01574 Genomic DNA. Translation: AAA88416.1 .
X02397 Genomic DNA. Translation: CAA26241.1 .
X01162 Genomic DNA. Translation: CAA25607.1 .
PIRi S07269. NRECE3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E44 X-ray 2.40 B 456-551 [» ]
1JCH X-ray 3.02 A/C 1-551 [» ]
1UJW X-ray 2.75 B 314-448 [» ]
2B5U X-ray 2.30 A/C 1-551 [» ]
2XFZ X-ray 3.20 Y 455-551 [» ]
2XG1 X-ray 3.20 Y 455-551 [» ]
ProteinModelPortali P00646.
SMRi P00646. Positions 82-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-545N.
IntActi P00646. 2 interactions.
MINTi MINT-145709.

Proteomic databases

PRIDEi P00646.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00646.

Family and domain databases

Gene3Di 1.10.287.620. 1 hit.
3.10.380.10. 1 hit.
InterProi IPR024575. Cloacin_colicin_fam.
IPR009105. Colicin_E3_ribonuclease.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view ]
Pfami PF09000. Cytotoxic. 1 hit.
PF11570. E2R135. 1 hit.
PF06958. Pyocin_S. 1 hit.
[Graphical view ]
PRINTSi PR01295. CLOACIN.
SUPFAMi SSF63840. SSF63840. 1 hit.
SSF69369. SSF69369. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3."
    Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P.
    Nucleic Acids Res. 12:8733-8745(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 370-551.
  3. "Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin."
    Mock M., Miyada C.G., Gunsalus R.P.
    Nucleic Acids Res. 11:3547-3557(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-551.
  4. "A plasmid region encoding the active fragment and the inhibitor protein of colicin E3-CA38."
    Masaki H., Ohta T.
    FEBS Lett. 149:129-132(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 445-551.
  5. "Amino acid sequence of an active fragment (T2A) of colicin E3."
    Suzuki K., Imahori K.
    J. Biochem. 84:1031-1039(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 455-551.

Entry informationi

Entry nameiCEA3_ECOLX
AccessioniPrimary (citable) accession number: P00646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3