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P00646

- CEA3_ECOLX

UniProt

P00646 - CEA3_ECOLX

Protein

Colicin-E3

Gene

ceaC

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.
    Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. ribosome binding Source: InterPro
    4. RNA binding Source: InterPro

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Colicin-E3 (EC:3.1.-.-)
    Alternative name(s):
    Colicin-E3 A chain
    Ribonuclease
    Gene namesi
    Name:ceaC
    Encoded oniPlasmid ColE3-CA380 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    GO - Cellular componenti

    1. extrachromosomal circular DNA Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Colicin-E3PRO_0000218677Add
    BLAST

    Proteomic databases

    PRIDEiP00646.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    immP029842EBI-1029919,EBI-1029912

    Protein-protein interaction databases

    DIPiDIP-545N.
    IntActiP00646. 2 interactions.
    MINTiMINT-145709.

    Structurei

    Secondary structure

    1
    551
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi93 – 964
    Beta strandi102 – 1087
    Helixi114 – 12310
    Beta strandi128 – 1303
    Beta strandi132 – 1398
    Helixi141 – 1433
    Beta strandi147 – 1504
    Beta strandi154 – 1607
    Helixi161 – 1644
    Beta strandi165 – 1673
    Helixi169 – 1713
    Beta strandi177 – 1826
    Beta strandi184 – 1929
    Beta strandi194 – 2029
    Beta strandi204 – 2107
    Beta strandi211 – 2144
    Beta strandi220 – 2223
    Beta strandi230 – 2378
    Beta strandi239 – 2413
    Beta strandi248 – 2503
    Beta strandi253 – 2553
    Turni261 – 2666
    Beta strandi267 – 2737
    Helixi276 – 2783
    Beta strandi283 – 2897
    Helixi293 – 31321
    Helixi315 – 37561
    Helixi376 – 3783
    Beta strandi382 – 3854
    Helixi386 – 44863
    Helixi457 – 4604
    Turni461 – 4633
    Helixi470 – 4723
    Beta strandi475 – 4773
    Beta strandi480 – 4823
    Beta strandi489 – 4924
    Beta strandi497 – 5004
    Turni501 – 5044
    Beta strandi505 – 5106
    Turni511 – 5144
    Beta strandi515 – 5206
    Turni521 – 5233
    Beta strandi525 – 5306
    Turni532 – 5343
    Beta strandi537 – 5393
    Helixi548 – 5503

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E44X-ray2.40B456-551[»]
    1JCHX-ray3.02A/C1-551[»]
    1UJWX-ray2.75B314-448[»]
    2B5UX-ray2.30A/C1-551[»]
    2XFZX-ray3.20Y455-551[»]
    2XG1X-ray3.20Y455-551[»]
    ProteinModelPortaliP00646.
    SMRiP00646. Positions 82-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00646.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni455 – 55197Ribosome inactivating activityAdd
    BLAST
    Regioni530 – 55122Binding of immunity proteinAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cloacin colicin family.Curated

    Family and domain databases

    Gene3Di1.10.287.620. 1 hit.
    3.10.380.10. 1 hit.
    InterProiIPR024575. Cloacin_colicin_fam.
    IPR009105. Colicin_E3_ribonuclease.
    IPR024566. Colicin_R_dom.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view]
    PfamiPF09000. Cytotoxic. 1 hit.
    PF11570. E2R135. 1 hit.
    PF06958. Pyocin_S. 1 hit.
    [Graphical view]
    PRINTSiPR01295. CLOACIN.
    SUPFAMiSSF63840. SSF63840. 1 hit.
    SSF69369. SSF69369. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGGDGRGHN TGAHSTSGNI NGGPTGLGVG GGASDGSGWS SENNPWGGGS    50
    GSGIHWGGGS GHGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA 100
    GGLAVSISAG ALSAAIADIM AALKGPFKFG LWGVALYGVL PSQIAKDDPN 150
    MMSKIVTSLP ADDITESPVS SLPLDKATVN VNVRVVDDVK DERQNISVVS 200
    GVPMSVPVVD AKPTERPGVF TASIPGAPVL NISVNNSTPA VQTLSPGVTN 250
    NTDKDVRPAG FTQGGNTRDA VIRFPKDSGH NAVYVSVSDV LSPDQVKQRQ 300
    DEENRRQQEW DATHPVEAAE RNYERARAEL NQANEDVARN QERQAKAVQV 350
    YNSRKSELDA ANKTLADAIA EIKQFNRFAH DPMAGGHRMW QMAGLKAQRA 400
    QTDVNNKQAA FDAAAKEKSD ADAALSSAME SRKKKEDKKR SAENNLNDEK 450
    NKPRKGFKDY GHDYHPAPKT ENIKGLGDLK PGIPKTPKQN GGGKRKRWTG 500
    DKGRKIYEWD SQHGELEGYR ASDGQHLGSF DPKTGNQLKG PDPKRNIKKY 550
    L 551
    Length:551
    Mass (Da):57,963
    Last modified:April 1, 1988 - v1
    Checksum:iE444CE918D89ECD6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01574 Genomic DNA. Translation: AAA88416.1.
    X02397 Genomic DNA. Translation: CAA26241.1.
    X01162 Genomic DNA. Translation: CAA25607.1.
    PIRiS07269. NRECE3.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01574 Genomic DNA. Translation: AAA88416.1 .
    X02397 Genomic DNA. Translation: CAA26241.1 .
    X01162 Genomic DNA. Translation: CAA25607.1 .
    PIRi S07269. NRECE3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E44 X-ray 2.40 B 456-551 [» ]
    1JCH X-ray 3.02 A/C 1-551 [» ]
    1UJW X-ray 2.75 B 314-448 [» ]
    2B5U X-ray 2.30 A/C 1-551 [» ]
    2XFZ X-ray 3.20 Y 455-551 [» ]
    2XG1 X-ray 3.20 Y 455-551 [» ]
    ProteinModelPortali P00646.
    SMRi P00646. Positions 82-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-545N.
    IntActi P00646. 2 interactions.
    MINTi MINT-145709.

    Proteomic databases

    PRIDEi P00646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P00646.

    Family and domain databases

    Gene3Di 1.10.287.620. 1 hit.
    3.10.380.10. 1 hit.
    InterProi IPR024575. Cloacin_colicin_fam.
    IPR009105. Colicin_E3_ribonuclease.
    IPR024566. Colicin_R_dom.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view ]
    Pfami PF09000. Cytotoxic. 1 hit.
    PF11570. E2R135. 1 hit.
    PF06958. Pyocin_S. 1 hit.
    [Graphical view ]
    PRINTSi PR01295. CLOACIN.
    SUPFAMi SSF63840. SSF63840. 1 hit.
    SSF69369. SSF69369. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3."
      Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P.
      Nucleic Acids Res. 12:8733-8745(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 370-551.
    3. "Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin."
      Mock M., Miyada C.G., Gunsalus R.P.
      Nucleic Acids Res. 11:3547-3557(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-551.
    4. "A plasmid region encoding the active fragment and the inhibitor protein of colicin E3-CA38."
      Masaki H., Ohta T.
      FEBS Lett. 149:129-132(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 445-551.
    5. "Amino acid sequence of an active fragment (T2A) of colicin E3."
      Suzuki K., Imahori K.
      J. Biochem. 84:1031-1039(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 455-551.

    Entry informationi

    Entry nameiCEA3_ECOLX
    AccessioniPrimary (citable) accession number: P00646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3