ID NUC_STAAU Reviewed; 231 AA. AC P00644; P13718; Q53711; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 21-JUL-1986, sequence version 1. DT 27-MAR-2024, entry version 193. DE RecName: Full=Thermonuclease; DE Short=TNase; DE EC=3.1.31.1; DE AltName: Full=Micrococcal nuclease; DE AltName: Full=Staphylococcal nuclease {ECO:0000303|PubMed:4324890}; DE Contains: DE RecName: Full=Nuclease B {ECO:0000303|PubMed:893427}; DE Contains: DE RecName: Full=Nuclease A {ECO:0000303|PubMed:893427}; DE Flags: Precursor; GN Name=nuc {ECO:0000303|PubMed:6307819}; OS Staphylococcus aureus. OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae; OC Staphylococcus. OX NCBI_TaxID=1280; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 27735 / Foggie; RX PubMed=6307819; DOI=10.1016/0378-1119(83)90102-6; RA Shortle D.; RT "A genetic system for analysis of staphylococcal nuclease."; RL Gene 22:181-189(1983). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112. RX PubMed=3921523; DOI=10.1128/jb.162.2.521-528.1985; RA Kovacevic S., Veal L.E., Hsiung H.M., Miller J.R.; RT "Secretion of staphylococcal nuclease by Bacillus subtilis."; RL J. Bacteriol. 162:521-528(1985). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-63. RX PubMed=3112123; DOI=10.1128/jb.169.8.3508-3514.1987; RA Miller J.R., Kovacevic S., Veal L.E.; RT "Secretion and processing of staphylococcal nuclease by Bacillus RT subtilis."; RL J. Bacteriol. 169:3508-3514(1987). RN [4] RP PROTEIN SEQUENCE OF 64-82, AND SUBCELLULAR LOCATION. RC STRAIN=ATCC 27735 / Foggie; RX PubMed=893427; DOI=10.1016/s0021-9258(17)39992-1; RA Davis A., Moore I.B., Parker D.S., Taniuchi H.; RT "Nuclease B. A possible precursor of nuclease A, an extracellular nuclease RT of Staphylococcus aureus."; RL J. Biol. Chem. 252:6544-6553(1977). RN [5] RP PROTEIN SEQUENCE OF 83-231, AND SUBCELLULAR LOCATION. RC STRAIN=ATCC 27735 / Foggie; RX PubMed=4324890; DOI=10.1016/s0021-9258(18)62201-x; RA Cone J.L., Cusumano C.L., Taniuchi H., Anfinsen C.B.; RT "Staphylococcal nuclease (Foggi strain). II. The amino acid sequence."; RL J. Biol. Chem. 246:3103-3110(1971). RN [6] RP AMIDE ASSIGNMENTS FOR 83-231. RC STRAIN=ATCC 27735 / Foggie; RX PubMed=4339720; DOI=10.1016/s0021-9258(19)45023-0; RA Bohnert J.L., Taniuchi H.; RT "The examination of the presence of amide groups in glutamic acid and RT aspartic acid residues of staphylococcal nuclease (Foggi strain)."; RL J. Biol. Chem. 247:4557-4560(1972). RN [7] RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), ACTIVE SITE, AND PROPOSED CATALYTIC RP MECHANISM. RX PubMed=288045; DOI=10.1073/pnas.76.6.2551; RA Cotton F.A., Hazen E.C. Jr., Legg M.J.; RT "Staphylococcal nuclease: proposed mechanism of action based on structure RT of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A RT resolution."; RL Proc. Natl. Acad. Sci. U.S.A. 76:2551-2555(1979). RN [8] RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS). RX PubMed=1896431; DOI=10.1002/prot.340100203; RA Hynes T.R., Fox R.O.; RT "The crystal structure of staphylococcal nuclease refined at 1.7-A RT resolution."; RL Proteins 10:92-105(1991). RN [9] RP STRUCTURE BY NMR. RX PubMed=1731948; DOI=10.1021/bi00118a038; RA Wang J., Mooberry E.S., Walkenhorst W.F., Markley J.L.; RT "Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N RT resonances and secondary structure of the unligated enzyme as identified by RT three-dimensional NMR spectroscopy."; RL Biochemistry 31:911-920(1992). RN [10] RP STRUCTURE BY NMR OF 83-218. RX PubMed=8069625; DOI=10.1016/0969-2126(93)90027-e; RA Shortle D., Abeygunawardana C.; RT "NMR analysis of the residual structure in the denatured state of an RT unusual mutant of staphylococcal nuclease."; RL Structure 1:121-134(1993). CC -!- FUNCTION: Enzyme that catalyzes the hydrolysis of both DNA and RNA at CC the 5' position of the phosphodiester bond. CC -!- CATALYTIC ACTIVITY: CC Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'- CC phosphooligonucleotide end-products.; EC=3.1.31.1; CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10048, ECO:0000255|PROSITE- CC ProRule:PRU10049}; CC -!- COFACTOR: CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; CC Note=Binds 1 Ca(2+) ion per subunit.; CC -!- SUBCELLULAR LOCATION: [Nuclease A]: Secreted CC {ECO:0000269|PubMed:893427, ECO:0000305|PubMed:4324890}. CC -!- SUBCELLULAR LOCATION: [Nuclease B]: Membrane. CC -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE- CC ProRule:PRU00272}. CC -!- WEB RESOURCE: Name=Worthington enzyme manual; CC URL="https://www.worthington-biochem.com/NFCP/"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; V01281; CAA24594.1; -; mRNA. DR EMBL; M17123; AAA26659.1; -; Genomic_DNA. DR PIR; A00790; NCSAF. DR PDB; 1A2T; X-ray; 1.96 A; A=83-231. DR PDB; 1A2U; X-ray; 2.00 A; A=83-231. DR PDB; 1A3T; X-ray; 2.10 A; A=83-231. DR PDB; 1A3U; X-ray; 2.05 A; A=83-231. DR PDB; 1A3V; X-ray; 2.20 A; A=83-231. DR PDB; 1AEX; X-ray; 2.10 A; A=83-231. DR PDB; 1ENA; X-ray; 2.15 A; A=89-223. DR PDB; 1ENC; X-ray; 1.95 A; A=83-231. DR PDB; 1EQV; X-ray; 1.94 A; A=88-223. DR PDB; 1EY0; X-ray; 1.60 A; A=83-231. DR PDB; 1EY4; X-ray; 1.60 A; A=83-231. DR PDB; 1EY5; X-ray; 1.70 A; A=83-231. DR PDB; 1EY6; X-ray; 1.75 A; A=83-231. DR PDB; 1EY7; X-ray; 1.88 A; A=83-231. DR PDB; 1EY8; X-ray; 1.75 A; A=83-231. DR PDB; 1EY9; X-ray; 1.72 A; A=83-231. DR PDB; 1EYA; X-ray; 2.00 A; A=83-231. DR PDB; 1EYC; X-ray; 1.85 A; A=83-231. DR PDB; 1EYD; X-ray; 1.70 A; A=83-231. DR PDB; 1EZ6; X-ray; 1.90 A; A=83-231. DR PDB; 1EZ8; X-ray; 1.85 A; A=83-231. DR PDB; 1F2M; X-ray; 2.00 A; A=83-231. DR PDB; 1F2Y; X-ray; 2.00 A; A=83-231. DR PDB; 1F2Z; X-ray; 1.80 A; A=83-231. DR PDB; 1IHZ; X-ray; 1.65 A; A=83-231. DR PDB; 1II3; X-ray; 1.72 A; A=83-231. DR PDB; 1JOK; NMR; -; A=83-231. DR PDB; 1JOO; NMR; -; A=83-231. DR PDB; 1JOQ; NMR; -; A=83-231. DR PDB; 1JOR; NMR; -; A=83-231. DR PDB; 1KAA; X-ray; 1.90 A; A=88-223. DR PDB; 1KAB; X-ray; 1.80 A; A=88-223. DR PDB; 1KDA; X-ray; 1.90 A; A=83-231. DR PDB; 1KDB; X-ray; 1.90 A; A=83-231. DR PDB; 1KDC; X-ray; 2.00 A; A=83-231. DR PDB; 1NSN; X-ray; 2.80 A; S=83-231. DR PDB; 1NUC; X-ray; 1.90 A; A=83-231. DR PDB; 1RKN; NMR; -; A=83-192. DR PDB; 1SNC; X-ray; 1.65 A; A=83-231. DR PDB; 1SND; X-ray; 1.84 A; A/B=83-231. DR PDB; 1SNM; X-ray; 1.74 A; A=83-231. DR PDB; 1SNO; X-ray; 1.70 A; A=83-231. DR PDB; 1SNP; X-ray; 1.95 A; A=83-231. DR PDB; 1SNQ; X-ray; 1.70 A; A=83-231. DR PDB; 1STA; X-ray; 1.70 A; A=83-231. DR PDB; 1STB; X-ray; 2.00 A; A=83-230. DR PDB; 1STG; X-ray; 1.70 A; A=83-231. DR PDB; 1STH; X-ray; 1.85 A; A=83-231. DR PDB; 1STN; X-ray; 1.70 A; A=83-231. DR PDB; 1STY; X-ray; 1.67 A; A=83-231. DR PDB; 1SYB; X-ray; 1.80 A; A=83-231. DR PDB; 1SYC; X-ray; 1.80 A; A=83-231. DR PDB; 1SYD; X-ray; 1.70 A; A=83-231. DR PDB; 1SYE; X-ray; 1.80 A; A=83-231. DR PDB; 1SYF; X-ray; 1.80 A; A=83-231. DR PDB; 1SYG; X-ray; 1.90 A; A=83-231. DR PDB; 1TQO; X-ray; 2.00 A; A=83-226. DR PDB; 1TR5; X-ray; 2.10 A; A=83-226. DR PDB; 1TT2; X-ray; 1.85 A; A=83-226. DR PDB; 1U9R; X-ray; 2.10 A; A=83-231. DR PDB; 2ENB; X-ray; 2.05 A; A=89-223. DR PDB; 2EXZ; X-ray; 1.90 A; A=83-231. DR PDB; 2EY1; X-ray; 1.85 A; A=83-231. DR PDB; 2EY2; X-ray; 1.70 A; A=83-231. DR PDB; 2EY5; X-ray; 2.00 A; A=83-231. DR PDB; 2EY6; X-ray; 1.65 A; A=83-231. DR PDB; 2EYF; X-ray; 1.80 A; A=83-231. DR PDB; 2EYH; X-ray; 1.90 A; A=83-231. DR PDB; 2EYJ; X-ray; 1.85 A; A=83-231. DR PDB; 2EYL; X-ray; 1.70 A; A=83-231. DR PDB; 2EYM; X-ray; 1.80 A; A=83-231. DR PDB; 2EYO; X-ray; 1.70 A; A=83-231. DR PDB; 2EYP; X-ray; 1.80 A; A=83-231. DR PDB; 2F0D; X-ray; 2.50 A; A=83-231. DR PDB; 2F0E; X-ray; 1.95 A; A=83-231. DR PDB; 2F0F; X-ray; 2.15 A; A=83-231. DR PDB; 2F0G; X-ray; 1.90 A; A=83-231. DR PDB; 2F0H; X-ray; 2.15 A; A=83-231. DR PDB; 2F0I; X-ray; 1.80 A; A=83-231. DR PDB; 2F0J; X-ray; 1.80 A; A=83-231. DR PDB; 2F0K; X-ray; 1.90 A; A=83-231. DR PDB; 2F0L; X-ray; 1.90 A; A=83-231. DR PDB; 2F0M; X-ray; 2.00 A; A=83-231. DR PDB; 2F0N; X-ray; 1.70 A; A=83-231. DR PDB; 2F0O; X-ray; 1.90 A; A=83-231. DR PDB; 2F0P; X-ray; 2.05 A; A=83-231. DR PDB; 2F0Q; X-ray; 1.95 A; A=83-231. DR PDB; 2F0S; X-ray; 1.75 A; A=83-231. DR PDB; 2F0T; X-ray; 1.70 A; A=83-231. DR PDB; 2F0U; X-ray; 1.83 A; A=83-231. DR PDB; 2F0V; X-ray; 2.00 A; A=83-231. DR PDB; 2F0W; X-ray; 2.10 A; A=83-231. DR PDB; 2F3V; NMR; -; A=83-192. DR PDB; 2F3W; NMR; -; A=83-192. DR PDB; 2FXY; NMR; -; A=137-154. DR PDB; 2FXZ; NMR; -; A=179-191. DR PDB; 2GSI; X-ray; 2.81 A; W/X/Y/Z=126-136. DR PDB; 2KHS; NMR; -; A=83-203. DR PDB; 2NUC; X-ray; 2.00 A; A=83-231. DR PDB; 2OEO; X-ray; 2.00 A; A=83-226. DR PDB; 2OF1; X-ray; 1.92 A; A=83-226. DR PDB; 2PQE; NMR; -; A=83-231. DR PDB; 2QDB; X-ray; 2.20 A; A=83-231. DR PDB; 2RBM; X-ray; 1.90 A; A=83-231. DR PDB; 2RDF; X-ray; 2.01 A; A=83-231. DR PDB; 2RKS; X-ray; 2.01 A; A=83-231. DR PDB; 2SNM; X-ray; 1.97 A; A=83-231. DR PDB; 2SNS; X-ray; 1.50 A; A=83-231. DR PDB; 2SOB; NMR; -; A=83-185. DR PDB; 3BDC; X-ray; 1.80 A; A=83-231. DR PDB; 3C1E; X-ray; 1.90 A; A=83-231. DR PDB; 3C1F; X-ray; 2.00 A; A=83-231. DR PDB; 3D4D; X-ray; 2.10 A; A/B=83-231. DR PDB; 3D6C; X-ray; 2.00 A; A=83-231. DR PDB; 3DHQ; X-ray; 2.15 A; A=83-231. DR PDB; 3DMU; X-ray; 1.80 A; A=83-231. DR PDB; 3E5S; X-ray; 2.00 A; A/B=83-231. DR PDB; 3EJI; X-ray; 1.90 A; A=83-231. DR PDB; 3ERO; X-ray; 1.85 A; A=83-231. DR PDB; 3ERQ; X-ray; 2.10 A; A=83-231. DR PDB; 3EVQ; X-ray; 2.00 A; A=83-231. DR PDB; 3H6M; X-ray; 1.70 A; A=83-231. DR PDB; 3HEJ; X-ray; 1.80 A; A/B/C/D=83-231. DR PDB; 3HZX; X-ray; 2.00 A; A=83-231. DR PDB; 3ITP; X-ray; 1.75 A; A=83-231. DR PDB; 3LX0; X-ray; 1.50 A; A=83-231. DR PDB; 3MEH; X-ray; 1.50 A; A=83-231. DR PDB; 3MHB; X-ray; 1.55 A; A=83-231. DR PDB; 3MXP; X-ray; 1.61 A; A=83-231. DR PDB; 3MZ5; X-ray; 1.58 A; A=83-231. DR PDB; 3NHH; X-ray; 2.00 A; A=83-231. DR PDB; 3NK9; X-ray; 1.65 A; A=83-231. DR PDB; 3NP8; X-ray; 1.70 A; A=83-231. DR PDB; 3NQT; X-ray; 1.90 A; A=83-231. DR PDB; 3NUC; X-ray; 1.90 A; A=83-231. DR PDB; 3NXW; X-ray; 1.65 A; A=83-231. DR PDB; 3OSO; X-ray; 1.60 A; A=83-231. DR PDB; 3OWF; X-ray; 1.85 A; A=83-231. DR PDB; 3P1H; X-ray; 1.79 A; A=83-231. DR PDB; 3P75; X-ray; 1.90 A; A=89-223. DR PDB; 3PMF; X-ray; 1.60 A; A=83-231. DR PDB; 3QB3; X-ray; 1.63 A; A=83-231. DR PDB; 3RUZ; X-ray; 1.58 A; A=83-231. DR PDB; 3S9W; X-ray; 1.90 A; A=83-231. DR PDB; 3SHL; X-ray; 1.48 A; A=83-231. DR PDB; 3SK4; X-ray; 1.85 A; A=83-231. DR PDB; 3SK5; X-ray; 1.70 A; A=83-231. DR PDB; 3SK6; X-ray; 1.80 A; A=83-231. DR PDB; 3SK8; X-ray; 1.90 A; A=83-231. DR PDB; 3SR1; X-ray; 1.45 A; A=83-231. DR PDB; 3SXH; X-ray; 1.40 A; A=83-231. DR PDB; 3T13; X-ray; 1.80 A; A/B=83-231. DR PDB; 3T16; X-ray; 1.80 A; A=83-231. DR PDB; 3TME; X-ray; 1.40 A; A=83-231. DR PDB; 3TP5; X-ray; 1.80 A; A=83-231. DR PDB; 3TP6; X-ray; 1.80 A; A=83-231. DR PDB; 3TP7; X-ray; 1.90 A; A=83-231. DR PDB; 3TP8; X-ray; 1.60 A; A=83-231. DR PDB; 3U9O; X-ray; 1.95 A; A=83-231. DR PDB; 3V2T; X-ray; 1.80 A; A=83-231. DR PDB; 3VA5; X-ray; 1.55 A; A=83-231. DR PDB; 4DF7; X-ray; 1.50 A; A=83-231. DR PDB; 4DFA; X-ray; 1.40 A; A=83-231. DR PDB; 4DGZ; X-ray; 1.47 A; A=83-231. DR PDB; 4DU9; X-ray; 1.63 A; A=83-231. DR PDB; 4E6I; X-ray; 2.00 A; A/B=83-231. DR PDB; 4EQN; X-ray; 1.80 A; A=83-231. DR PDB; 4EQO; X-ray; 1.70 A; A=83-231. DR PDB; 4EQP; X-ray; 1.35 A; A=83-231. DR PDB; 4EVO; X-ray; 1.95 A; A=83-231. DR PDB; 4F7X; X-ray; 1.70 A; A=83-231. DR PDB; 4F8M; X-ray; 1.60 A; A=83-231. DR PDB; 4G57; X-ray; 1.81 A; A=89-223. DR PDB; 4H7B; X-ray; 1.60 A; A=83-231. DR PDB; 4HMI; X-ray; 1.75 A; A=83-231. DR PDB; 4HMJ; X-ray; 1.35 A; A=83-231. DR PDB; 4HTH; X-ray; 1.75 A; A=83-231. DR PDB; 4I65; X-ray; 1.61 A; A=88-223. DR PDB; 4IAL; X-ray; 1.60 A; A=83-231. DR PDB; 4ID6; X-ray; 1.90 A; A=83-231. DR PDB; 4IUN; X-ray; 1.60 A; A=83-231. DR PDB; 4IZ8; X-ray; 1.70 A; A=83-231. DR PDB; 4J1M; X-ray; 1.65 A; A=83-231. DR PDB; 4J6H; X-ray; 1.60 A; A=83-231. DR PDB; 4K14; X-ray; 1.60 A; A=88-223. DR PDB; 4K2K; X-ray; 1.65 A; A=83-231. DR PDB; 4K2L; X-ray; 1.55 A; A=83-231. DR PDB; 4K5V; X-ray; 1.75 A; A=83-231. DR PDB; 4K5W; X-ray; 1.65 A; A=83-231. DR PDB; 4K5X; X-ray; 1.65 A; A=83-231. DR PDB; 4K6D; X-ray; 1.65 A; A=83-231. DR PDB; 4K8I; X-ray; 2.10 A; A=89-223. DR PDB; 4K8J; X-ray; 2.00 A; A=89-223. DR PDB; 4KD3; X-ray; 1.75 A; A=83-231. DR PDB; 4KD4; X-ray; 1.55 A; A=83-231. DR PDB; 4KHV; X-ray; 1.60 A; A=83-231. DR PDB; 4KJN; X-ray; 1.55 A; A=83-231. DR PDB; 4KJO; X-ray; 1.90 A; A=83-231. DR PDB; 4KTA; X-ray; 1.95 A; A=83-231. DR PDB; 4KV6; X-ray; 1.55 A; A=83-231. DR PDB; 4KY5; X-ray; 1.40 A; A=83-231. DR PDB; 4KY6; X-ray; 1.70 A; A=83-231. DR PDB; 4KY7; X-ray; 1.55 A; A=83-231. DR PDB; 4LAA; X-ray; 1.58 A; A=83-231. DR PDB; 4ME5; X-ray; 1.80 A; A=83-231. DR PDB; 4MIU; X-ray; 1.67 A; A=83-231. DR PDB; 4N9P; X-ray; 1.40 A; A=83-231. DR PDB; 4N9T; X-ray; 1.60 A; A=83-231. DR PDB; 4NDX; X-ray; 1.50 A; A=83-231. DR PDB; 4NKL; X-ray; 1.60 A; A=83-231. DR PDB; 4NMZ; X-ray; 1.70 A; A=83-231. DR PDB; 4NP5; X-ray; 1.50 A; A=83-231. DR PDB; 4ODG; X-ray; 1.73 A; A=83-231. DR PDB; 4OL7; X-ray; 1.67 A; A/B=83-231. DR PDB; 4PMB; X-ray; 1.80 A; A=83-231. DR PDB; 4PMC; X-ray; 1.65 A; A=83-231. DR PDB; 4PNY; X-ray; 1.68 A; A=83-231. DR PDB; 4QB4; X-ray; 1.60 A; A=88-223. DR PDB; 4QF4; X-ray; 1.80 A; A/B=83-231. DR PDB; 4R8N; X-ray; 1.65 A; A=83-223. DR PDB; 4RKB; X-ray; 1.88 A; A=83-231. DR PDB; 4RKL; X-ray; 1.66 A; A=83-231. DR PDB; 4S3S; X-ray; 1.64 A; A=83-231. DR PDB; 4TRD; X-ray; 1.60 A; A=83-231. DR PDB; 4WOR; X-ray; 1.60 A; A=83-231. DR PDB; 4YIJ; X-ray; 1.64 A; A/B=83-231. DR PDB; 4ZQ3; X-ray; 1.90 A; A=83-231. DR PDB; 4ZUI; X-ray; 1.70 A; A=83-231. DR PDB; 4ZUJ; X-ray; 1.72 A; A=83-231. DR PDB; 5C3W; X-ray; 1.72 A; A=83-231. DR PDB; 5C3X; X-ray; 1.95 A; A=83-231. DR PDB; 5C4H; X-ray; 1.80 A; A=83-231. DR PDB; 5C4Z; X-ray; 1.62 A; A=83-231. DR PDB; 5C5L; X-ray; 1.75 A; A=83-231. DR PDB; 5C5M; X-ray; 1.70 A; A=83-231. DR PDB; 5C6A; X-ray; 1.85 A; A=83-231. DR PDB; 5CC4; X-ray; 1.90 A; A=83-231. DR PDB; 5CGK; X-ray; 1.85 A; A=83-231. DR PDB; 5CR3; X-ray; 1.80 A; A=83-231. DR PDB; 5CV4; X-ray; 1.80 A; A=83-231. DR PDB; 5CV5; X-ray; 1.80 A; A/B=83-231. DR PDB; 5CV6; X-ray; 1.95 A; A=83-231. DR PDB; 5CV7; X-ray; 1.65 A; A=83-231. DR PDB; 5CV8; X-ray; 1.58 A; A=83-231. DR PDB; 5CV9; X-ray; 1.62 A; A=83-231. DR PDB; 5DEH; X-ray; 1.70 A; A=83-231. DR PDB; 5E1F; X-ray; 1.90 A; A=83-231. DR PDB; 5E3F; X-ray; 1.75 A; A=83-231. DR PDB; 5EGT; X-ray; 1.85 A; A=83-231. DR PDB; 5EKK; X-ray; 2.10 A; A=83-231. DR PDB; 5EKL; X-ray; 2.10 A; A=83-231. DR PDB; 5F2D; X-ray; 1.70 A; A=83-231. DR PDB; 5HGT; X-ray; 1.75 A; A=83-231. DR PDB; 5HUR; X-ray; 1.50 A; A=83-231. DR PDB; 5I6W; X-ray; 1.90 A; A=83-231. DR PDB; 5I6Y; X-ray; 1.90 A; A=83-231. DR PDB; 5I9O; X-ray; 1.95 A; A/B=83-231. DR PDB; 5I9P; X-ray; 1.85 A; A=83-231. DR PDB; 5IGB; X-ray; 1.80 A; A=83-231. DR PDB; 5IGC; X-ray; 1.80 A; A=83-231. DR PDB; 5IGD; X-ray; 1.80 A; A=83-231. DR PDB; 5IGE; X-ray; 1.80 A; A=83-231. DR PDB; 5IGF; X-ray; 1.70 A; A=83-231. DR PDB; 5IGG; X-ray; 1.70 A; A=83-231. DR PDB; 5IIF; X-ray; 1.85 A; A=83-231. DR PDB; 5IOC; X-ray; 1.90 A; A=83-231. DR PDB; 5IOD; X-ray; 1.60 A; A=83-231. DR PDB; 5ISR; X-ray; 1.90 A; A=83-231. DR PDB; 5J1Z; X-ray; 1.73 A; A=83-231. DR PDB; 5J22; X-ray; 1.90 A; A=83-231. DR PDB; 5JAV; X-ray; 1.89 A; A=83-231. DR PDB; 5JOB; X-ray; 1.45 A; A=83-231. DR PDB; 5K5P; X-ray; 1.83 A; A=83-231. DR PDB; 5KEE; X-ray; 1.50 A; A=83-231. DR PDB; 5KGU; X-ray; 1.90 A; A=83-231. DR PDB; 5KIX; X-ray; 1.75 A; A=83-231. DR PDB; 5KRU; X-ray; 1.60 A; A=83-231. DR PDB; 5KYI; X-ray; 1.70 A; A=83-231. DR PDB; 5KYL; X-ray; 1.85 A; A=83-231. DR PDB; 5NUC; X-ray; 2.10 A; A=83-231. DR PDB; 6AMF; X-ray; 1.85 A; A=83-231. DR PDB; 6B8R; X-ray; 1.65 A; A=83-231. DR PDB; 6EEG; X-ray; 1.95 A; A=83-231. DR PDB; 6EEK; X-ray; 2.20 A; A=83-231. DR PDB; 6OK8; X-ray; 1.80 A; A=83-231. DR PDB; 6U0W; X-ray; 1.90 A; A=83-231. DR PDB; 6U0X; X-ray; 1.86 A; A=83-231. DR PDB; 6XSB; X-ray; 1.95 A; A=83-231. DR PDB; 6XSC; X-ray; 2.00 A; A=83-231. DR PDB; 6XSE; X-ray; 2.10 A; A=83-231. DR PDB; 6XSF; X-ray; 1.60 A; A=83-231. DR PDB; 6XSG; X-ray; 2.00 A; A=83-231. DR PDB; 6XSH; X-ray; 2.00 A; A=83-231. DR PDB; 6XSI; X-ray; 2.00 A; A=83-231. DR PDBsum; 1A2T; -. DR PDBsum; 1A2U; -. DR PDBsum; 1A3T; -. DR PDBsum; 1A3U; -. DR PDBsum; 1A3V; -. DR PDBsum; 1AEX; -. DR PDBsum; 1ENA; -. DR PDBsum; 1ENC; -. DR PDBsum; 1EQV; -. DR PDBsum; 1EY0; -. DR PDBsum; 1EY4; -. DR PDBsum; 1EY5; -. DR PDBsum; 1EY6; -. DR PDBsum; 1EY7; -. DR PDBsum; 1EY8; -. DR PDBsum; 1EY9; -. DR PDBsum; 1EYA; -. DR PDBsum; 1EYC; -. DR PDBsum; 1EYD; -. DR PDBsum; 1EZ6; -. DR PDBsum; 1EZ8; -. DR PDBsum; 1F2M; -. DR PDBsum; 1F2Y; -. DR PDBsum; 1F2Z; -. DR PDBsum; 1IHZ; -. DR PDBsum; 1II3; -. DR PDBsum; 1JOK; -. DR PDBsum; 1JOO; -. DR PDBsum; 1JOQ; -. DR PDBsum; 1JOR; -. DR PDBsum; 1KAA; -. DR PDBsum; 1KAB; -. DR PDBsum; 1KDA; -. DR PDBsum; 1KDB; -. DR PDBsum; 1KDC; -. DR PDBsum; 1NSN; -. DR PDBsum; 1NUC; -. DR PDBsum; 1RKN; -. DR PDBsum; 1SNC; -. DR PDBsum; 1SND; -. DR PDBsum; 1SNM; -. DR PDBsum; 1SNO; -. DR PDBsum; 1SNP; -. DR PDBsum; 1SNQ; -. DR PDBsum; 1STA; -. DR PDBsum; 1STB; -. DR PDBsum; 1STG; -. DR PDBsum; 1STH; -. DR PDBsum; 1STN; -. DR PDBsum; 1STY; -. DR PDBsum; 1SYB; -. DR PDBsum; 1SYC; -. DR PDBsum; 1SYD; -. DR PDBsum; 1SYE; -. DR PDBsum; 1SYF; -. DR PDBsum; 1SYG; -. DR PDBsum; 1TQO; -. DR PDBsum; 1TR5; -. DR PDBsum; 1TT2; -. DR PDBsum; 1U9R; -. DR PDBsum; 2ENB; -. DR PDBsum; 2EXZ; -. DR PDBsum; 2EY1; -. DR PDBsum; 2EY2; -. DR PDBsum; 2EY5; -. DR PDBsum; 2EY6; -. DR PDBsum; 2EYF; -. DR PDBsum; 2EYH; -. DR PDBsum; 2EYJ; -. DR PDBsum; 2EYL; -. DR PDBsum; 2EYM; -. DR PDBsum; 2EYO; -. DR PDBsum; 2EYP; -. DR PDBsum; 2F0D; -. DR PDBsum; 2F0E; -. DR PDBsum; 2F0F; -. DR PDBsum; 2F0G; -. DR PDBsum; 2F0H; -. DR PDBsum; 2F0I; -. DR PDBsum; 2F0J; -. DR PDBsum; 2F0K; -. DR PDBsum; 2F0L; -. DR PDBsum; 2F0M; -. DR PDBsum; 2F0N; -. DR PDBsum; 2F0O; -. DR PDBsum; 2F0P; -. DR PDBsum; 2F0Q; -. DR PDBsum; 2F0S; -. DR PDBsum; 2F0T; -. DR PDBsum; 2F0U; -. DR PDBsum; 2F0V; -. DR PDBsum; 2F0W; -. DR PDBsum; 2F3V; -. DR PDBsum; 2F3W; -. DR PDBsum; 2FXY; -. DR PDBsum; 2FXZ; -. DR PDBsum; 2GSI; -. DR PDBsum; 2KHS; -. DR PDBsum; 2NUC; -. DR PDBsum; 2OEO; -. DR PDBsum; 2OF1; -. DR PDBsum; 2PQE; -. DR PDBsum; 2QDB; -. DR PDBsum; 2RBM; -. DR PDBsum; 2RDF; -. DR PDBsum; 2RKS; -. DR PDBsum; 2SNM; -. DR PDBsum; 2SNS; -. DR PDBsum; 2SOB; -. DR PDBsum; 3BDC; -. DR PDBsum; 3C1E; -. DR PDBsum; 3C1F; -. DR PDBsum; 3D4D; -. DR PDBsum; 3D6C; -. DR PDBsum; 3DHQ; -. DR PDBsum; 3DMU; -. DR PDBsum; 3E5S; -. DR PDBsum; 3EJI; -. DR PDBsum; 3ERO; -. DR PDBsum; 3ERQ; -. DR PDBsum; 3EVQ; -. DR PDBsum; 3H6M; -. DR PDBsum; 3HEJ; -. DR PDBsum; 3HZX; -. DR PDBsum; 3ITP; -. DR PDBsum; 3LX0; -. DR PDBsum; 3MEH; -. DR PDBsum; 3MHB; -. DR PDBsum; 3MXP; -. DR PDBsum; 3MZ5; -. DR PDBsum; 3NHH; -. DR PDBsum; 3NK9; -. DR PDBsum; 3NP8; -. DR PDBsum; 3NQT; -. DR PDBsum; 3NUC; -. DR PDBsum; 3NXW; -. DR PDBsum; 3OSO; -. DR PDBsum; 3OWF; -. DR PDBsum; 3P1H; -. DR PDBsum; 3P75; -. DR PDBsum; 3PMF; -. DR PDBsum; 3QB3; -. DR PDBsum; 3RUZ; -. DR PDBsum; 3S9W; -. DR PDBsum; 3SHL; -. DR PDBsum; 3SK4; -. DR PDBsum; 3SK5; -. DR PDBsum; 3SK6; -. DR PDBsum; 3SK8; -. DR PDBsum; 3SR1; -. DR PDBsum; 3SXH; -. DR PDBsum; 3T13; -. DR PDBsum; 3T16; -. DR PDBsum; 3TME; -. DR PDBsum; 3TP5; -. DR PDBsum; 3TP6; -. DR PDBsum; 3TP7; -. DR PDBsum; 3TP8; -. DR PDBsum; 3U9O; -. DR PDBsum; 3V2T; -. DR PDBsum; 3VA5; -. DR PDBsum; 4DF7; -. DR PDBsum; 4DFA; -. DR PDBsum; 4DGZ; -. DR PDBsum; 4DU9; -. DR PDBsum; 4E6I; -. DR PDBsum; 4EQN; -. DR PDBsum; 4EQO; -. DR PDBsum; 4EQP; -. DR PDBsum; 4EVO; -. DR PDBsum; 4F7X; -. DR PDBsum; 4F8M; -. DR PDBsum; 4G57; -. DR PDBsum; 4H7B; -. DR PDBsum; 4HMI; -. DR PDBsum; 4HMJ; -. DR PDBsum; 4HTH; -. DR PDBsum; 4I65; -. DR PDBsum; 4IAL; -. DR PDBsum; 4ID6; -. DR PDBsum; 4IUN; -. DR PDBsum; 4IZ8; -. DR PDBsum; 4J1M; -. DR PDBsum; 4J6H; -. DR PDBsum; 4K14; -. DR PDBsum; 4K2K; -. DR PDBsum; 4K2L; -. DR PDBsum; 4K5V; -. DR PDBsum; 4K5W; -. DR PDBsum; 4K5X; -. DR PDBsum; 4K6D; -. DR PDBsum; 4K8I; -. DR PDBsum; 4K8J; -. DR PDBsum; 4KD3; -. DR PDBsum; 4KD4; -. DR PDBsum; 4KHV; -. DR PDBsum; 4KJN; -. DR PDBsum; 4KJO; -. DR PDBsum; 4KTA; -. DR PDBsum; 4KV6; -. DR PDBsum; 4KY5; -. DR PDBsum; 4KY6; -. DR PDBsum; 4KY7; -. DR PDBsum; 4LAA; -. DR PDBsum; 4ME5; -. DR PDBsum; 4MIU; -. DR PDBsum; 4N9P; -. DR PDBsum; 4N9T; -. DR PDBsum; 4NDX; -. DR PDBsum; 4NKL; -. DR PDBsum; 4NMZ; -. DR PDBsum; 4NP5; -. DR PDBsum; 4ODG; -. DR PDBsum; 4OL7; -. DR PDBsum; 4PMB; -. DR PDBsum; 4PMC; -. DR PDBsum; 4PNY; -. DR PDBsum; 4QB4; -. DR PDBsum; 4QF4; -. DR PDBsum; 4R8N; -. DR PDBsum; 4RKB; -. DR PDBsum; 4RKL; -. DR PDBsum; 4S3S; -. DR PDBsum; 4TRD; -. DR PDBsum; 4WOR; -. DR PDBsum; 4YIJ; -. DR PDBsum; 4ZQ3; -. DR PDBsum; 4ZUI; -. DR PDBsum; 4ZUJ; -. DR PDBsum; 5C3W; -. DR PDBsum; 5C3X; -. DR PDBsum; 5C4H; -. DR PDBsum; 5C4Z; -. DR PDBsum; 5C5L; -. DR PDBsum; 5C5M; -. DR PDBsum; 5C6A; -. DR PDBsum; 5CC4; -. DR PDBsum; 5CGK; -. DR PDBsum; 5CR3; -. DR PDBsum; 5CV4; -. DR PDBsum; 5CV5; -. DR PDBsum; 5CV6; -. DR PDBsum; 5CV7; -. DR PDBsum; 5CV8; -. DR PDBsum; 5CV9; -. DR PDBsum; 5DEH; -. DR PDBsum; 5E1F; -. DR PDBsum; 5E3F; -. DR PDBsum; 5EGT; -. DR PDBsum; 5EKK; -. DR PDBsum; 5EKL; -. DR PDBsum; 5F2D; -. DR PDBsum; 5HGT; -. DR PDBsum; 5HUR; -. DR PDBsum; 5I6W; -. DR PDBsum; 5I6Y; -. DR PDBsum; 5I9O; -. DR PDBsum; 5I9P; -. DR PDBsum; 5IGB; -. DR PDBsum; 5IGC; -. DR PDBsum; 5IGD; -. DR PDBsum; 5IGE; -. DR PDBsum; 5IGF; -. DR PDBsum; 5IGG; -. DR PDBsum; 5IIF; -. DR PDBsum; 5IOC; -. DR PDBsum; 5IOD; -. DR PDBsum; 5ISR; -. DR PDBsum; 5J1Z; -. DR PDBsum; 5J22; -. DR PDBsum; 5JAV; -. DR PDBsum; 5JOB; -. DR PDBsum; 5K5P; -. DR PDBsum; 5KEE; -. DR PDBsum; 5KGU; -. DR PDBsum; 5KIX; -. DR PDBsum; 5KRU; -. DR PDBsum; 5KYI; -. DR PDBsum; 5KYL; -. DR PDBsum; 5NUC; -. DR PDBsum; 6AMF; -. DR PDBsum; 6B8R; -. DR PDBsum; 6EEG; -. DR PDBsum; 6EEK; -. DR PDBsum; 6OK8; -. DR PDBsum; 6U0W; -. DR PDBsum; 6U0X; -. DR PDBsum; 6XSB; -. DR PDBsum; 6XSC; -. DR PDBsum; 6XSE; -. DR PDBsum; 6XSF; -. DR PDBsum; 6XSG; -. DR PDBsum; 6XSH; -. DR PDBsum; 6XSI; -. DR AlphaFoldDB; P00644; -. DR BMRB; P00644; -. DR SMR; P00644; -. DR DrugBank; DB03654; S,S-Propylthiocysteine. DR DrugBank; DB04205; Thymidine-3',5'-Diphosphate. DR ABCD; P00644; 2 sequenced antibodies. DR BRENDA; 3.1.31.1; 3352. DR EvolutionaryTrace; P00644; -. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016894; F:endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters; IEA:UniProtKB-EC. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR CDD; cd00175; SNc; 1. DR Gene3D; 2.40.50.90; -; 1. DR InterPro; IPR035437; SNase_OB-fold_sf. DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold. DR InterPro; IPR002071; Thermonucl_AS. DR PANTHER; PTHR12302; EBNA2 BINDING PROTEIN P100; 1. DR PANTHER; PTHR12302:SF3; ENDONUCLEASE LCL3-RELATED; 1. DR Pfam; PF00565; SNase; 1. DR SMART; SM00318; SNc; 1. DR SUPFAM; SSF50199; Staphylococcal nuclease; 1. DR PROSITE; PS01123; TNASE_1; 1. DR PROSITE; PS01284; TNASE_2; 1. DR PROSITE; PS50830; TNASE_3; 1. PE 1: Evidence at protein level; KW 3D-structure; Calcium; Direct protein sequencing; Endonuclease; Hydrolase; KW Membrane; Metal-binding; Nuclease; Secreted; Signal; Zymogen. FT SIGNAL 1..26 FT /evidence="ECO:0000255" FT PROPEP 27..63 FT /evidence="ECO:0000269|PubMed:893427" FT /id="PRO_0000045226" FT CHAIN 64..231 FT /note="Nuclease B" FT /id="PRO_0000034389" FT PROPEP 64..82 FT /evidence="ECO:0000269|PubMed:4324890" FT /id="PRO_0000034390" FT CHAIN 83..231 FT /note="Nuclease A" FT /id="PRO_0000034391" FT REGION 61..86 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 203..231 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 203..223 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 117 FT /evidence="ECO:0000269|PubMed:288045" FT ACT_SITE 125 FT /evidence="ECO:0000269|PubMed:288045" FT ACT_SITE 169 FT /evidence="ECO:0000269|PubMed:288045" FT BINDING 103 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT BINDING 122 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT BINDING 123 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT CONFLICT 30 FT /note="V -> M (in Ref. 3; AAA26659)" FT /evidence="ECO:0000305" FT TURN 84..86 FT /evidence="ECO:0007829|PDB:2SNS" FT STRAND 91..99 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 101..109 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 112..118 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 126..128 FT /evidence="ECO:0007829|PDB:4H7B" FT STRAND 129..131 FT /evidence="ECO:0007829|PDB:2SNS" FT STRAND 134..136 FT /evidence="ECO:0007829|PDB:1JOK" FT HELIX 137..150 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 154..157 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 166..168 FT /evidence="ECO:0007829|PDB:1NSN" FT STRAND 170..176 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 177..179 FT /evidence="ECO:0007829|PDB:2SOB" FT HELIX 181..187 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 190..193 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 198..200 FT /evidence="ECO:0007829|PDB:2SNS" FT TURN 201..203 FT /evidence="ECO:0007829|PDB:1ENA" FT HELIX 204..216 FT /evidence="ECO:0007829|PDB:4EQP" FT HELIX 220..222 FT /evidence="ECO:0007829|PDB:4EQP" FT STRAND 227..229 FT /evidence="ECO:0007829|PDB:1JOK" SQ SEQUENCE 231 AA; 25471 MW; AD5558AA28E855D7 CRC64; MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT LVVVSSLSSS ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI DGDTVKLMYK GQPMTFRLLL VDTPETKHPK KGVEKYGPEA SAFTKKMVEN AKKIEVEFDK GQRTDKYGRG LAYIYADGKM VNEALVRQGL AKVAYVYKPN NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q //