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P00644

- NUC_STAAU

UniProt

P00644 - NUC_STAAU

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Protein
Thermonuclease
Gene
nuc
Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Cofactori

Binds 1 calcium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Calcium
Active sitei117 – 11711 Publication
Metal bindingi122 – 1221Calcium
Metal bindingi123 – 1231Calcium; via carbonyl oxygen
Active sitei125 – 12511 Publication
Active sitei169 – 16911 Publication

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Cleaved into the following 2 chains:
Gene namesi
Name:nuc
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Propeptidei27 – 6337
PRO_0000045226Add
BLAST
Chaini64 – 231168Nuclease B
PRO_0000034389Add
BLAST
Propeptidei64 – 8219
PRO_0000034390Add
BLAST
Chaini83 – 231149Nuclease A
PRO_0000034391Add
BLAST

Keywords - PTMi

Zymogen

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni84 – 863
Beta strandi91 – 999
Beta strandi101 – 1099
Beta strandi112 – 1187
Beta strandi120 – 1223
Beta strandi126 – 1283
Beta strandi129 – 1313
Beta strandi134 – 1363
Helixi137 – 15014
Beta strandi154 – 1574
Beta strandi166 – 1683
Beta strandi170 – 1767
Beta strandi177 – 1793
Helixi181 – 1877
Beta strandi190 – 1934
Beta strandi198 – 2003
Turni201 – 2033
Helixi204 – 21613
Helixi220 – 2223
Beta strandi227 – 2293

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2TX-ray1.96A83-231[»]
1A2UX-ray2.00A83-231[»]
1A3TX-ray2.10A83-231[»]
1A3UX-ray2.05A83-231[»]
1A3VX-ray2.20A83-231[»]
1AEXX-ray2.10A83-231[»]
1ENAX-ray2.15A89-223[»]
1ENCX-ray1.95A83-231[»]
1EQVX-ray1.94A88-223[»]
1EY0X-ray1.60A83-231[»]
1EY4X-ray1.60A83-231[»]
1EY5X-ray1.70A83-231[»]
1EY6X-ray1.75A83-231[»]
1EY7X-ray1.88A83-231[»]
1EY8X-ray1.75A83-231[»]
1EY9X-ray1.72A83-231[»]
1EYAX-ray2.00A83-231[»]
1EYCX-ray1.85A83-231[»]
1EYDX-ray1.70A83-231[»]
1EZ6X-ray1.90A83-231[»]
1EZ8X-ray1.85A83-231[»]
1F2MX-ray2.00A83-231[»]
1F2YX-ray2.00A83-231[»]
1F2ZX-ray1.80A83-231[»]
1IHZX-ray1.65A83-231[»]
1II3X-ray1.72A83-231[»]
1JOKNMR-A83-231[»]
1JOONMR-A83-231[»]
1JOQNMR-A83-231[»]
1JORNMR-A83-231[»]
1KAAX-ray1.90A88-223[»]
1KABX-ray1.80A88-223[»]
1KDAX-ray1.90A83-231[»]
1KDBX-ray1.90A83-231[»]
1KDCX-ray2.00A83-231[»]
1NSNX-ray2.80S83-231[»]
1NUCX-ray1.90A83-231[»]
1RKNNMR-A83-192[»]
1SNCX-ray1.65A83-231[»]
1SNDX-ray1.84A/B83-231[»]
1SNMX-ray1.74A83-231[»]
1SNOX-ray1.70A83-231[»]
1SNPX-ray1.95A83-231[»]
1SNQX-ray1.70A83-231[»]
1STAX-ray1.70A83-229[»]
1STBX-ray2.00A83-230[»]
1STGX-ray1.70A83-231[»]
1STHX-ray1.85A83-231[»]
1STNX-ray1.70A83-231[»]
1STYX-ray1.67A83-230[»]
1SYBX-ray1.80A83-231[»]
1SYCX-ray1.80A83-231[»]
1SYDX-ray1.70A83-231[»]
1SYEX-ray1.80A83-231[»]
1SYFX-ray1.80A83-231[»]
1SYGX-ray1.90A83-231[»]
1TQOX-ray2.00A83-226[»]
1TR5X-ray2.10A83-226[»]
1TT2X-ray1.85A83-226[»]
1U9RX-ray2.10A83-231[»]
2ENBX-ray2.05A89-223[»]
2EXZX-ray1.90A83-231[»]
2EY1X-ray1.85A83-231[»]
2EY2X-ray1.70A83-231[»]
2EY5X-ray2.00A83-231[»]
2EY6X-ray1.65A83-231[»]
2EYFX-ray1.80A83-231[»]
2EYHX-ray1.90A83-231[»]
2EYJX-ray1.85A83-231[»]
2EYLX-ray1.70A83-231[»]
2EYMX-ray1.80A83-231[»]
2EYOX-ray1.70A83-231[»]
2EYPX-ray1.80A83-231[»]
2F0DX-ray2.50A83-231[»]
2F0EX-ray1.95A83-231[»]
2F0FX-ray2.15A83-231[»]
2F0GX-ray1.90A83-231[»]
2F0HX-ray2.15A83-231[»]
2F0IX-ray1.80A83-231[»]
2F0JX-ray1.80A83-231[»]
2F0KX-ray1.90A83-231[»]
2F0LX-ray1.90A83-231[»]
2F0MX-ray2.00A83-231[»]
2F0NX-ray1.70A83-231[»]
2F0OX-ray1.90A83-231[»]
2F0PX-ray2.05A83-231[»]
2F0QX-ray1.95A83-231[»]
2F0SX-ray1.75A83-231[»]
2F0TX-ray1.70A83-231[»]
2F0UX-ray1.83A83-231[»]
2F0VX-ray2.00A83-231[»]
2F0WX-ray2.10A83-231[»]
2F3VNMR-A83-192[»]
2F3WNMR-A83-192[»]
2FXYNMR-A137-154[»]
2FXZNMR-A179-191[»]
2GSIX-ray2.81W/X/Y/Z126-136[»]
2KHSNMR-A83-203[»]
2NUCX-ray2.00A83-231[»]
2OEOX-ray2.00A83-226[»]
2OF1X-ray1.92A83-226[»]
2PQENMR-A83-231[»]
2QDBX-ray2.20A83-231[»]
2RBMX-ray1.90A83-231[»]
2RDFX-ray2.01A83-231[»]
2RKSX-ray2.01A83-231[»]
2SNMX-ray1.97A83-231[»]
2SNSX-ray1.50A83-231[»]
2SOBNMR-A83-185[»]
3BDCX-ray1.80A83-231[»]
3C1EX-ray1.90A83-231[»]
3C1FX-ray2.00A83-231[»]
3D4DX-ray2.10A/B83-231[»]
3D6CX-ray2.00A83-231[»]
3DHQX-ray2.15A83-231[»]
3DMUX-ray1.80A83-231[»]
3E5SX-ray2.00A/B83-231[»]
3EJIX-ray1.90A83-231[»]
3EROX-ray1.85A83-231[»]
3ERQX-ray2.10A83-231[»]
3EVQX-ray2.00A83-231[»]
3H6MX-ray1.70A83-231[»]
3HEJX-ray1.80A/B/C/D83-231[»]
3HZXX-ray2.00A83-231[»]
3ITPX-ray1.75A83-231[»]
3LX0X-ray1.50A83-231[»]
3MEHX-ray1.50A83-231[»]
3MHBX-ray1.55A83-231[»]
3MXPX-ray1.61A83-231[»]
3MZ5X-ray1.58A83-231[»]
3NHHX-ray2.00A83-231[»]
3NK9X-ray1.65A83-231[»]
3NP8X-ray1.70A83-231[»]
3NQTX-ray1.90A83-231[»]
3NUCX-ray1.90A83-231[»]
3NXWX-ray1.65A83-231[»]
3OSOX-ray1.60A83-231[»]
3OWFX-ray1.85A83-231[»]
3P1HX-ray1.79A83-231[»]
3P75X-ray1.90A89-223[»]
3PMFX-ray1.60A83-231[»]
3QB3X-ray1.63A83-231[»]
3RUZX-ray1.58A83-231[»]
3S9WX-ray1.90A83-231[»]
3SHLX-ray1.48A83-231[»]
3SK4X-ray1.85A83-231[»]
3SK5X-ray1.70A83-231[»]
3SK6X-ray1.80A83-231[»]
3SK8X-ray1.90A83-231[»]
3SR1X-ray1.45A83-231[»]
3SXHX-ray1.40A83-231[»]
3T13X-ray1.80A/B83-231[»]
3T16X-ray1.80A83-231[»]
3TMEX-ray1.40A83-231[»]
3TP5X-ray1.80A83-231[»]
3TP6X-ray1.80A83-231[»]
3TP7X-ray1.90A83-231[»]
3TP8X-ray1.60A83-231[»]
3U9OX-ray1.95A83-231[»]
3V2TX-ray1.80A83-231[»]
3VA5X-ray1.55A83-231[»]
4DF7X-ray1.50A83-231[»]
4DFAX-ray1.40A83-231[»]
4DGZX-ray1.47A83-231[»]
4DU9X-ray1.63A83-231[»]
4E6IX-ray2.00A/B83-231[»]
4EQNX-ray1.80A83-231[»]
4EQOX-ray1.70A83-231[»]
4EQPX-ray1.35A83-231[»]
4EVOX-ray1.95A83-231[»]
4F7XX-ray1.70A83-231[»]
4F8MX-ray1.60A83-231[»]
4G57X-ray1.81A89-223[»]
4H7BX-ray1.60A83-231[»]
4HMIX-ray1.75A83-231[»]
4HMJX-ray1.35A83-231[»]
4HTHX-ray1.75A83-231[»]
4I65X-ray1.61A88-223[»]
4IALX-ray1.60A83-231[»]
4ID6X-ray1.90A83-231[»]
4IUNX-ray1.60A83-231[»]
4IZ8X-ray1.70A83-231[»]
4J1MX-ray1.65A83-231[»]
4J6HX-ray1.60A83-231[»]
4K14X-ray1.60A88-223[»]
4K2KX-ray1.65A83-231[»]
4K2LX-ray1.55A83-231[»]
4K5VX-ray1.75A83-231[»]
4K5WX-ray1.65A83-231[»]
4K5XX-ray1.65A83-231[»]
4K6DX-ray1.65A83-231[»]
4K8IX-ray2.10A89-223[»]
4K8JX-ray2.00A89-223[»]
4KD3X-ray1.75A83-231[»]
4KD4X-ray1.55A83-231[»]
4KHVX-ray1.60A83-231[»]
4KJNX-ray1.55A83-231[»]
4KJOX-ray1.90A83-231[»]
4KTAX-ray1.95A83-231[»]
4KV6X-ray1.55A83-231[»]
4KY5X-ray1.40A83-231[»]
4KY6X-ray1.70A83-231[»]
4KY7X-ray1.55A83-231[»]
4LAAX-ray1.58A83-231[»]
4ME5X-ray1.80A83-231[»]
4MIUX-ray1.67A83-231[»]
4N9PX-ray1.40A83-231[»]
4N9TX-ray1.60A83-231[»]
4NDXX-ray1.50A83-231[»]
4NKLX-ray1.60A83-231[»]
4NMZX-ray1.70A83-231[»]
4NP5X-ray1.50A83-231[»]
4ODGX-ray1.73A83-231[»]
4PNYX-ray1.68A83-231[»]
5NUCX-ray2.10A83-231[»]
ProteinModelPortaliP00644.
SMRiP00644. Positions 86-223.

Miscellaneous databases

EvolutionaryTraceiP00644.

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1525.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00644-1 [UniParc]FASTAAdd to Basket

« Hide

MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT    50
LVVVSSLSSS ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI 100
DGDTVKLMYK GQPMTFRLLL VDTPETKHPK KGVEKYGPEA SAFTKKMVEN 150
AKKIEVEFDK GQRTDKYGRG LAYIYADGKM VNEALVRQGL AKVAYVYKPN 200
NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q 231
Length:231
Mass (Da):25,471
Last modified:July 21, 1986 - v1
Checksum:iAD5558AA28E855D7
GO

Sequence cautioni

The sequence AAA26659.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01281 mRNA. Translation: CAA24594.1.
M17123 Genomic DNA. Translation: AAA26659.1. Different initiation.
PIRiA00790. NCSAF.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01281 mRNA. Translation: CAA24594.1 .
M17123 Genomic DNA. Translation: AAA26659.1 . Different initiation.
PIRi A00790. NCSAF.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A2T X-ray 1.96 A 83-231 [» ]
1A2U X-ray 2.00 A 83-231 [» ]
1A3T X-ray 2.10 A 83-231 [» ]
1A3U X-ray 2.05 A 83-231 [» ]
1A3V X-ray 2.20 A 83-231 [» ]
1AEX X-ray 2.10 A 83-231 [» ]
1ENA X-ray 2.15 A 89-223 [» ]
1ENC X-ray 1.95 A 83-231 [» ]
1EQV X-ray 1.94 A 88-223 [» ]
1EY0 X-ray 1.60 A 83-231 [» ]
1EY4 X-ray 1.60 A 83-231 [» ]
1EY5 X-ray 1.70 A 83-231 [» ]
1EY6 X-ray 1.75 A 83-231 [» ]
1EY7 X-ray 1.88 A 83-231 [» ]
1EY8 X-ray 1.75 A 83-231 [» ]
1EY9 X-ray 1.72 A 83-231 [» ]
1EYA X-ray 2.00 A 83-231 [» ]
1EYC X-ray 1.85 A 83-231 [» ]
1EYD X-ray 1.70 A 83-231 [» ]
1EZ6 X-ray 1.90 A 83-231 [» ]
1EZ8 X-ray 1.85 A 83-231 [» ]
1F2M X-ray 2.00 A 83-231 [» ]
1F2Y X-ray 2.00 A 83-231 [» ]
1F2Z X-ray 1.80 A 83-231 [» ]
1IHZ X-ray 1.65 A 83-231 [» ]
1II3 X-ray 1.72 A 83-231 [» ]
1JOK NMR - A 83-231 [» ]
1JOO NMR - A 83-231 [» ]
1JOQ NMR - A 83-231 [» ]
1JOR NMR - A 83-231 [» ]
1KAA X-ray 1.90 A 88-223 [» ]
1KAB X-ray 1.80 A 88-223 [» ]
1KDA X-ray 1.90 A 83-231 [» ]
1KDB X-ray 1.90 A 83-231 [» ]
1KDC X-ray 2.00 A 83-231 [» ]
1NSN X-ray 2.80 S 83-231 [» ]
1NUC X-ray 1.90 A 83-231 [» ]
1RKN NMR - A 83-192 [» ]
1SNC X-ray 1.65 A 83-231 [» ]
1SND X-ray 1.84 A/B 83-231 [» ]
1SNM X-ray 1.74 A 83-231 [» ]
1SNO X-ray 1.70 A 83-231 [» ]
1SNP X-ray 1.95 A 83-231 [» ]
1SNQ X-ray 1.70 A 83-231 [» ]
1STA X-ray 1.70 A 83-229 [» ]
1STB X-ray 2.00 A 83-230 [» ]
1STG X-ray 1.70 A 83-231 [» ]
1STH X-ray 1.85 A 83-231 [» ]
1STN X-ray 1.70 A 83-231 [» ]
1STY X-ray 1.67 A 83-230 [» ]
1SYB X-ray 1.80 A 83-231 [» ]
1SYC X-ray 1.80 A 83-231 [» ]
1SYD X-ray 1.70 A 83-231 [» ]
1SYE X-ray 1.80 A 83-231 [» ]
1SYF X-ray 1.80 A 83-231 [» ]
1SYG X-ray 1.90 A 83-231 [» ]
1TQO X-ray 2.00 A 83-226 [» ]
1TR5 X-ray 2.10 A 83-226 [» ]
1TT2 X-ray 1.85 A 83-226 [» ]
1U9R X-ray 2.10 A 83-231 [» ]
2ENB X-ray 2.05 A 89-223 [» ]
2EXZ X-ray 1.90 A 83-231 [» ]
2EY1 X-ray 1.85 A 83-231 [» ]
2EY2 X-ray 1.70 A 83-231 [» ]
2EY5 X-ray 2.00 A 83-231 [» ]
2EY6 X-ray 1.65 A 83-231 [» ]
2EYF X-ray 1.80 A 83-231 [» ]
2EYH X-ray 1.90 A 83-231 [» ]
2EYJ X-ray 1.85 A 83-231 [» ]
2EYL X-ray 1.70 A 83-231 [» ]
2EYM X-ray 1.80 A 83-231 [» ]
2EYO X-ray 1.70 A 83-231 [» ]
2EYP X-ray 1.80 A 83-231 [» ]
2F0D X-ray 2.50 A 83-231 [» ]
2F0E X-ray 1.95 A 83-231 [» ]
2F0F X-ray 2.15 A 83-231 [» ]
2F0G X-ray 1.90 A 83-231 [» ]
2F0H X-ray 2.15 A 83-231 [» ]
2F0I X-ray 1.80 A 83-231 [» ]
2F0J X-ray 1.80 A 83-231 [» ]
2F0K X-ray 1.90 A 83-231 [» ]
2F0L X-ray 1.90 A 83-231 [» ]
2F0M X-ray 2.00 A 83-231 [» ]
2F0N X-ray 1.70 A 83-231 [» ]
2F0O X-ray 1.90 A 83-231 [» ]
2F0P X-ray 2.05 A 83-231 [» ]
2F0Q X-ray 1.95 A 83-231 [» ]
2F0S X-ray 1.75 A 83-231 [» ]
2F0T X-ray 1.70 A 83-231 [» ]
2F0U X-ray 1.83 A 83-231 [» ]
2F0V X-ray 2.00 A 83-231 [» ]
2F0W X-ray 2.10 A 83-231 [» ]
2F3V NMR - A 83-192 [» ]
2F3W NMR - A 83-192 [» ]
2FXY NMR - A 137-154 [» ]
2FXZ NMR - A 179-191 [» ]
2GSI X-ray 2.81 W/X/Y/Z 126-136 [» ]
2KHS NMR - A 83-203 [» ]
2NUC X-ray 2.00 A 83-231 [» ]
2OEO X-ray 2.00 A 83-226 [» ]
2OF1 X-ray 1.92 A 83-226 [» ]
2PQE NMR - A 83-231 [» ]
2QDB X-ray 2.20 A 83-231 [» ]
2RBM X-ray 1.90 A 83-231 [» ]
2RDF X-ray 2.01 A 83-231 [» ]
2RKS X-ray 2.01 A 83-231 [» ]
2SNM X-ray 1.97 A 83-231 [» ]
2SNS X-ray 1.50 A 83-231 [» ]
2SOB NMR - A 83-185 [» ]
3BDC X-ray 1.80 A 83-231 [» ]
3C1E X-ray 1.90 A 83-231 [» ]
3C1F X-ray 2.00 A 83-231 [» ]
3D4D X-ray 2.10 A/B 83-231 [» ]
3D6C X-ray 2.00 A 83-231 [» ]
3DHQ X-ray 2.15 A 83-231 [» ]
3DMU X-ray 1.80 A 83-231 [» ]
3E5S X-ray 2.00 A/B 83-231 [» ]
3EJI X-ray 1.90 A 83-231 [» ]
3ERO X-ray 1.85 A 83-231 [» ]
3ERQ X-ray 2.10 A 83-231 [» ]
3EVQ X-ray 2.00 A 83-231 [» ]
3H6M X-ray 1.70 A 83-231 [» ]
3HEJ X-ray 1.80 A/B/C/D 83-231 [» ]
3HZX X-ray 2.00 A 83-231 [» ]
3ITP X-ray 1.75 A 83-231 [» ]
3LX0 X-ray 1.50 A 83-231 [» ]
3MEH X-ray 1.50 A 83-231 [» ]
3MHB X-ray 1.55 A 83-231 [» ]
3MXP X-ray 1.61 A 83-231 [» ]
3MZ5 X-ray 1.58 A 83-231 [» ]
3NHH X-ray 2.00 A 83-231 [» ]
3NK9 X-ray 1.65 A 83-231 [» ]
3NP8 X-ray 1.70 A 83-231 [» ]
3NQT X-ray 1.90 A 83-231 [» ]
3NUC X-ray 1.90 A 83-231 [» ]
3NXW X-ray 1.65 A 83-231 [» ]
3OSO X-ray 1.60 A 83-231 [» ]
3OWF X-ray 1.85 A 83-231 [» ]
3P1H X-ray 1.79 A 83-231 [» ]
3P75 X-ray 1.90 A 89-223 [» ]
3PMF X-ray 1.60 A 83-231 [» ]
3QB3 X-ray 1.63 A 83-231 [» ]
3RUZ X-ray 1.58 A 83-231 [» ]
3S9W X-ray 1.90 A 83-231 [» ]
3SHL X-ray 1.48 A 83-231 [» ]
3SK4 X-ray 1.85 A 83-231 [» ]
3SK5 X-ray 1.70 A 83-231 [» ]
3SK6 X-ray 1.80 A 83-231 [» ]
3SK8 X-ray 1.90 A 83-231 [» ]
3SR1 X-ray 1.45 A 83-231 [» ]
3SXH X-ray 1.40 A 83-231 [» ]
3T13 X-ray 1.80 A/B 83-231 [» ]
3T16 X-ray 1.80 A 83-231 [» ]
3TME X-ray 1.40 A 83-231 [» ]
3TP5 X-ray 1.80 A 83-231 [» ]
3TP6 X-ray 1.80 A 83-231 [» ]
3TP7 X-ray 1.90 A 83-231 [» ]
3TP8 X-ray 1.60 A 83-231 [» ]
3U9O X-ray 1.95 A 83-231 [» ]
3V2T X-ray 1.80 A 83-231 [» ]
3VA5 X-ray 1.55 A 83-231 [» ]
4DF7 X-ray 1.50 A 83-231 [» ]
4DFA X-ray 1.40 A 83-231 [» ]
4DGZ X-ray 1.47 A 83-231 [» ]
4DU9 X-ray 1.63 A 83-231 [» ]
4E6I X-ray 2.00 A/B 83-231 [» ]
4EQN X-ray 1.80 A 83-231 [» ]
4EQO X-ray 1.70 A 83-231 [» ]
4EQP X-ray 1.35 A 83-231 [» ]
4EVO X-ray 1.95 A 83-231 [» ]
4F7X X-ray 1.70 A 83-231 [» ]
4F8M X-ray 1.60 A 83-231 [» ]
4G57 X-ray 1.81 A 89-223 [» ]
4H7B X-ray 1.60 A 83-231 [» ]
4HMI X-ray 1.75 A 83-231 [» ]
4HMJ X-ray 1.35 A 83-231 [» ]
4HTH X-ray 1.75 A 83-231 [» ]
4I65 X-ray 1.61 A 88-223 [» ]
4IAL X-ray 1.60 A 83-231 [» ]
4ID6 X-ray 1.90 A 83-231 [» ]
4IUN X-ray 1.60 A 83-231 [» ]
4IZ8 X-ray 1.70 A 83-231 [» ]
4J1M X-ray 1.65 A 83-231 [» ]
4J6H X-ray 1.60 A 83-231 [» ]
4K14 X-ray 1.60 A 88-223 [» ]
4K2K X-ray 1.65 A 83-231 [» ]
4K2L X-ray 1.55 A 83-231 [» ]
4K5V X-ray 1.75 A 83-231 [» ]
4K5W X-ray 1.65 A 83-231 [» ]
4K5X X-ray 1.65 A 83-231 [» ]
4K6D X-ray 1.65 A 83-231 [» ]
4K8I X-ray 2.10 A 89-223 [» ]
4K8J X-ray 2.00 A 89-223 [» ]
4KD3 X-ray 1.75 A 83-231 [» ]
4KD4 X-ray 1.55 A 83-231 [» ]
4KHV X-ray 1.60 A 83-231 [» ]
4KJN X-ray 1.55 A 83-231 [» ]
4KJO X-ray 1.90 A 83-231 [» ]
4KTA X-ray 1.95 A 83-231 [» ]
4KV6 X-ray 1.55 A 83-231 [» ]
4KY5 X-ray 1.40 A 83-231 [» ]
4KY6 X-ray 1.70 A 83-231 [» ]
4KY7 X-ray 1.55 A 83-231 [» ]
4LAA X-ray 1.58 A 83-231 [» ]
4ME5 X-ray 1.80 A 83-231 [» ]
4MIU X-ray 1.67 A 83-231 [» ]
4N9P X-ray 1.40 A 83-231 [» ]
4N9T X-ray 1.60 A 83-231 [» ]
4NDX X-ray 1.50 A 83-231 [» ]
4NKL X-ray 1.60 A 83-231 [» ]
4NMZ X-ray 1.70 A 83-231 [» ]
4NP5 X-ray 1.50 A 83-231 [» ]
4ODG X-ray 1.73 A 83-231 [» ]
4PNY X-ray 1.68 A 83-231 [» ]
5NUC X-ray 2.10 A 83-231 [» ]
ProteinModelPortali P00644.
SMRi P00644. Positions 86-223.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB01093. Dimethyl sulfoxide.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1525.

Miscellaneous databases

EvolutionaryTracei P00644.

Family and domain databases

Gene3Di 2.40.50.90. 1 hit.
InterProi IPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view ]
Pfami PF00565. SNase. 1 hit.
[Graphical view ]
SMARTi SM00318. SNc. 1 hit.
[Graphical view ]
SUPFAMi SSF50199. SSF50199. 1 hit.
PROSITEi PS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A genetic system for analysis of staphylococcal nuclease."
    Shortle D.
    Gene 22:181-189(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27735 / Foggie.
  2. "Secretion of staphylococcal nuclease by Bacillus subtilis."
    Kovacevic S., Veal L.E., Hsiung H.M., Miller J.R.
    J. Bacteriol. 162:521-528(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
  3. "Secretion and processing of staphylococcal nuclease by Bacillus subtilis."
    Miller J.R., Kovacevic S., Veal L.E.
    J. Bacteriol. 169:3508-3514(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-63.
  4. "Nuclease B. A possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus."
    Davis A., Moore I.B., Parker D.S., Taniuchi H.
    J. Biol. Chem. 252:6544-6553(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 64-82.
    Strain: ATCC 27735 / Foggie.
  5. "Staphylococcal nuclease (Foggi strain). II. The amino acid sequence."
    Cone J.L., Cusumano C.L., Taniuchi H., Anfinsen C.B.
    J. Biol. Chem. 246:3103-3110(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 83-231.
    Strain: ATCC 27735 / Foggie.
  6. "The examination of the presence of amide groups in glutamic acid and aspartic acid residues of staphylococcal nuclease (Foggi strain)."
    Bohnert J.L., Taniuchi H.
    J. Biol. Chem. 247:4557-4560(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: AMIDE ASSIGNMENTS FOR 83-231.
    Strain: ATCC 27735 / Foggie.
  7. "Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution."
    Cotton F.A., Hazen E.C. Jr., Legg M.J.
    Proc. Natl. Acad. Sci. U.S.A. 76:2551-2555(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), PROPOSED CATALYTIC MECHANISM.
  8. "The crystal structure of staphylococcal nuclease refined at 1.7-A resolution."
    Hynes T.R., Fox R.O.
    Proteins 10:92-105(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
  9. "Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N resonances and secondary structure of the unligated enzyme as identified by three-dimensional NMR spectroscopy."
    Wang J., Mooberry E.S., Walkenhorst W.F., Markley J.L.
    Biochemistry 31:911-920(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  10. "NMR analysis of the residual structure in the denatured state of an unusual mutant of staphylococcal nuclease."
    Shortle D., Abeygunawardana C.
    Structure 1:121-134(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 83-218.

Entry informationi

Entry nameiNUC_STAAU
AccessioniPrimary (citable) accession number: P00644
Secondary accession number(s): P13718, Q53711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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