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P00644

- NUC_STAAU

UniProt

P00644 - NUC_STAAU

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Protein
Thermonuclease
Gene
nuc
Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Cofactori

Binds 1 calcium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Calcium
Active sitei117 – 11711 Publication
Metal bindingi122 – 1221Calcium
Metal bindingi123 – 1231Calcium; via carbonyl oxygen
Active sitei125 – 12511 Publication
Active sitei169 – 16911 Publication

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thermonuclease (EC:3.1.31.1)
    Short name:
    TNase
    Alternative name(s):
    Micrococcal nuclease
    Staphylococcal nuclease
    Cleaved into the following 2 chains:
    Gene namesi
    Name:nuc
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane, Secreted

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626 Reviewed prediction
    Add
    BLAST
    Propeptidei27 – 6337
    PRO_0000045226Add
    BLAST
    Chaini64 – 231168Nuclease B
    PRO_0000034389Add
    BLAST
    Propeptidei64 – 8219
    PRO_0000034390Add
    BLAST
    Chaini83 – 231149Nuclease A
    PRO_0000034391Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni84 – 863
    Beta strandi91 – 999
    Beta strandi101 – 1099
    Beta strandi112 – 1187
    Beta strandi120 – 1223
    Beta strandi126 – 1283
    Beta strandi129 – 1313
    Beta strandi134 – 1363
    Helixi137 – 15014
    Beta strandi154 – 1574
    Beta strandi166 – 1683
    Beta strandi170 – 1767
    Beta strandi177 – 1793
    Helixi181 – 1877
    Beta strandi190 – 1934
    Beta strandi198 – 2003
    Turni201 – 2033
    Helixi204 – 21613
    Helixi220 – 2223
    Beta strandi227 – 2293

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A2TX-ray1.96A83-231[»]
    1A2UX-ray2.00A83-231[»]
    1A3TX-ray2.10A83-231[»]
    1A3UX-ray2.05A83-231[»]
    1A3VX-ray2.20A83-231[»]
    1AEXX-ray2.10A83-231[»]
    1ENAX-ray2.15A89-223[»]
    1ENCX-ray1.95A83-231[»]
    1EQVX-ray1.94A88-223[»]
    1EY0X-ray1.60A83-231[»]
    1EY4X-ray1.60A83-231[»]
    1EY5X-ray1.70A83-231[»]
    1EY6X-ray1.75A83-231[»]
    1EY7X-ray1.88A83-231[»]
    1EY8X-ray1.75A83-231[»]
    1EY9X-ray1.72A83-231[»]
    1EYAX-ray2.00A83-231[»]
    1EYCX-ray1.85A83-231[»]
    1EYDX-ray1.70A83-231[»]
    1EZ6X-ray1.90A83-231[»]
    1EZ8X-ray1.85A83-231[»]
    1F2MX-ray2.00A83-231[»]
    1F2YX-ray2.00A83-231[»]
    1F2ZX-ray1.80A83-231[»]
    1IHZX-ray1.65A83-231[»]
    1II3X-ray1.72A83-231[»]
    1JOKNMR-A83-231[»]
    1JOONMR-A83-231[»]
    1JOQNMR-A83-231[»]
    1JORNMR-A83-231[»]
    1KAAX-ray1.90A88-223[»]
    1KABX-ray1.80A88-223[»]
    1KDAX-ray1.90A83-231[»]
    1KDBX-ray1.90A83-231[»]
    1KDCX-ray2.00A83-231[»]
    1NSNX-ray2.80S83-231[»]
    1NUCX-ray1.90A83-231[»]
    1RKNNMR-A83-192[»]
    1SNCX-ray1.65A83-231[»]
    1SNDX-ray1.84A/B83-231[»]
    1SNMX-ray1.74A83-231[»]
    1SNOX-ray1.70A83-231[»]
    1SNPX-ray1.95A83-231[»]
    1SNQX-ray1.70A83-231[»]
    1STAX-ray1.70A83-229[»]
    1STBX-ray2.00A83-230[»]
    1STGX-ray1.70A83-231[»]
    1STHX-ray1.85A83-231[»]
    1STNX-ray1.70A83-231[»]
    1STYX-ray1.67A83-230[»]
    1SYBX-ray1.80A83-231[»]
    1SYCX-ray1.80A83-231[»]
    1SYDX-ray1.70A83-231[»]
    1SYEX-ray1.80A83-231[»]
    1SYFX-ray1.80A83-231[»]
    1SYGX-ray1.90A83-231[»]
    1TQOX-ray2.00A83-226[»]
    1TR5X-ray2.10A83-226[»]
    1TT2X-ray1.85A83-226[»]
    1U9RX-ray2.10A83-231[»]
    2ENBX-ray2.05A89-223[»]
    2EXZX-ray1.90A83-231[»]
    2EY1X-ray1.85A83-231[»]
    2EY2X-ray1.70A83-231[»]
    2EY5X-ray2.00A83-231[»]
    2EY6X-ray1.65A83-231[»]
    2EYFX-ray1.80A83-231[»]
    2EYHX-ray1.90A83-231[»]
    2EYJX-ray1.85A83-231[»]
    2EYLX-ray1.70A83-231[»]
    2EYMX-ray1.80A83-231[»]
    2EYOX-ray1.70A83-231[»]
    2EYPX-ray1.80A83-231[»]
    2F0DX-ray2.50A83-231[»]
    2F0EX-ray1.95A83-231[»]
    2F0FX-ray2.15A83-231[»]
    2F0GX-ray1.90A83-231[»]
    2F0HX-ray2.15A83-231[»]
    2F0IX-ray1.80A83-231[»]
    2F0JX-ray1.80A83-231[»]
    2F0KX-ray1.90A83-231[»]
    2F0LX-ray1.90A83-231[»]
    2F0MX-ray2.00A83-231[»]
    2F0NX-ray1.70A83-231[»]
    2F0OX-ray1.90A83-231[»]
    2F0PX-ray2.05A83-231[»]
    2F0QX-ray1.95A83-231[»]
    2F0SX-ray1.75A83-231[»]
    2F0TX-ray1.70A83-231[»]
    2F0UX-ray1.83A83-231[»]
    2F0VX-ray2.00A83-231[»]
    2F0WX-ray2.10A83-231[»]
    2F3VNMR-A83-192[»]
    2F3WNMR-A83-192[»]
    2FXYNMR-A137-154[»]
    2FXZNMR-A179-191[»]
    2GSIX-ray2.81W/X/Y/Z126-136[»]
    2KHSNMR-A83-203[»]
    2NUCX-ray2.00A83-231[»]
    2OEOX-ray2.00A83-226[»]
    2OF1X-ray1.92A83-226[»]
    2PQENMR-A83-231[»]
    2QDBX-ray2.20A83-231[»]
    2RBMX-ray1.90A83-231[»]
    2RDFX-ray2.01A83-231[»]
    2RKSX-ray2.01A83-231[»]
    2SNMX-ray1.97A83-231[»]
    2SNSX-ray1.50A83-231[»]
    2SOBNMR-A83-185[»]
    3BDCX-ray1.80A83-231[»]
    3C1EX-ray1.90A83-231[»]
    3C1FX-ray2.00A83-231[»]
    3D4DX-ray2.10A/B83-231[»]
    3D6CX-ray2.00A83-231[»]
    3DHQX-ray2.15A83-231[»]
    3DMUX-ray1.80A83-231[»]
    3E5SX-ray2.00A/B83-231[»]
    3EJIX-ray1.90A83-231[»]
    3EROX-ray1.85A83-231[»]
    3ERQX-ray2.10A83-231[»]
    3EVQX-ray2.00A83-231[»]
    3H6MX-ray1.70A83-231[»]
    3HEJX-ray1.80A/B/C/D83-231[»]
    3HZXX-ray2.00A83-231[»]
    3ITPX-ray1.75A83-231[»]
    3LX0X-ray1.50A83-231[»]
    3MEHX-ray1.50A83-231[»]
    3MHBX-ray1.55A83-231[»]
    3MXPX-ray1.61A83-231[»]
    3MZ5X-ray1.58A83-231[»]
    3NHHX-ray2.00A83-231[»]
    3NK9X-ray1.65A83-231[»]
    3NP8X-ray1.70A83-231[»]
    3NQTX-ray1.90A83-231[»]
    3NUCX-ray1.90A83-231[»]
    3NXWX-ray1.65A83-231[»]
    3OSOX-ray1.60A83-231[»]
    3OWFX-ray1.85A83-231[»]
    3P1HX-ray1.79A83-231[»]
    3P75X-ray1.90A89-223[»]
    3PMFX-ray1.60A83-231[»]
    3QB3X-ray1.63A83-231[»]
    3RUZX-ray1.58A83-231[»]
    3S9WX-ray1.90A83-231[»]
    3SHLX-ray1.48A83-231[»]
    3SK4X-ray1.85A83-231[»]
    3SK5X-ray1.70A83-231[»]
    3SK6X-ray1.80A83-231[»]
    3SK8X-ray1.90A83-231[»]
    3SR1X-ray1.45A83-231[»]
    3SXHX-ray1.40A83-231[»]
    3T13X-ray1.80A/B83-231[»]
    3T16X-ray1.80A83-231[»]
    3TMEX-ray1.40A83-231[»]
    3TP5X-ray1.80A83-231[»]
    3TP6X-ray1.80A83-231[»]
    3TP7X-ray1.90A83-231[»]
    3TP8X-ray1.60A83-231[»]
    3U9OX-ray1.95A83-231[»]
    3V2TX-ray1.80A83-231[»]
    3VA5X-ray1.55A83-231[»]
    4DF7X-ray1.50A83-231[»]
    4DFAX-ray1.40A83-231[»]
    4DGZX-ray1.47A83-231[»]
    4DU9X-ray1.63A83-231[»]
    4E6IX-ray2.00A/B83-231[»]
    4EQNX-ray1.80A83-231[»]
    4EQOX-ray1.70A83-231[»]
    4EQPX-ray1.35A83-231[»]
    4EVOX-ray1.95A83-231[»]
    4F7XX-ray1.70A83-231[»]
    4F8MX-ray1.60A83-231[»]
    4G57X-ray1.81A89-223[»]
    4H7BX-ray1.60A83-231[»]
    4HMIX-ray1.75A83-231[»]
    4HMJX-ray1.35A83-231[»]
    4HTHX-ray1.75A83-231[»]
    4I65X-ray1.61A88-223[»]
    4IALX-ray1.60A83-231[»]
    4ID6X-ray1.90A83-231[»]
    4IUNX-ray1.60A83-231[»]
    4IZ8X-ray1.70A83-231[»]
    4J1MX-ray1.65A83-231[»]
    4J6HX-ray1.60A83-231[»]
    4K14X-ray1.60A88-223[»]
    4K2KX-ray1.65A83-231[»]
    4K2LX-ray1.55A83-231[»]
    4K5VX-ray1.75A83-231[»]
    4K5WX-ray1.65A83-231[»]
    4K5XX-ray1.65A83-231[»]
    4K6DX-ray1.65A83-231[»]
    4K8IX-ray2.10A89-223[»]
    4K8JX-ray2.00A89-223[»]
    4KD3X-ray1.75A83-231[»]
    4KD4X-ray1.55A83-231[»]
    4KHVX-ray1.60A83-231[»]
    4KJNX-ray1.55A83-231[»]
    4KJOX-ray1.90A83-231[»]
    4KTAX-ray1.95A83-231[»]
    4KV6X-ray1.55A83-231[»]
    4KY5X-ray1.40A83-231[»]
    4KY6X-ray1.70A83-231[»]
    4KY7X-ray1.55A83-231[»]
    4LAAX-ray1.58A83-231[»]
    4ME5X-ray1.80A83-231[»]
    4MIUX-ray1.67A83-231[»]
    4N9PX-ray1.40A83-231[»]
    4N9TX-ray1.60A83-231[»]
    4NDXX-ray1.50A83-231[»]
    4NKLX-ray1.60A83-231[»]
    4NMZX-ray1.70A83-231[»]
    4NP5X-ray1.50A83-231[»]
    4ODGX-ray1.73A83-231[»]
    4PNYX-ray1.68A83-231[»]
    5NUCX-ray2.10A83-231[»]
    ProteinModelPortaliP00644.
    SMRiP00644. Positions 86-223.

    Miscellaneous databases

    EvolutionaryTraceiP00644.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thermonuclease family.

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1525.

    Family and domain databases

    Gene3Di2.40.50.90. 1 hit.
    InterProiIPR016071. Staphylococal_nuclease_OB-fold.
    IPR002071. Thermonucl_AS.
    [Graphical view]
    PfamiPF00565. SNase. 1 hit.
    [Graphical view]
    SMARTiSM00318. SNc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50199. SSF50199. 1 hit.
    PROSITEiPS01123. TNASE_1. 1 hit.
    PS01284. TNASE_2. 1 hit.
    PS50830. TNASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00644-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT    50
    LVVVSSLSSS ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI 100
    DGDTVKLMYK GQPMTFRLLL VDTPETKHPK KGVEKYGPEA SAFTKKMVEN 150
    AKKIEVEFDK GQRTDKYGRG LAYIYADGKM VNEALVRQGL AKVAYVYKPN 200
    NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q 231
    Length:231
    Mass (Da):25,471
    Last modified:July 21, 1986 - v1
    Checksum:iAD5558AA28E855D7
    GO

    Sequence cautioni

    The sequence AAA26659.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01281 mRNA. Translation: CAA24594.1.
    M17123 Genomic DNA. Translation: AAA26659.1. Different initiation.
    PIRiA00790. NCSAF.

    Cross-referencesi

    Web resourcesi

    Worthington enzyme manual

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01281 mRNA. Translation: CAA24594.1 .
    M17123 Genomic DNA. Translation: AAA26659.1 . Different initiation.
    PIRi A00790. NCSAF.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A2T X-ray 1.96 A 83-231 [» ]
    1A2U X-ray 2.00 A 83-231 [» ]
    1A3T X-ray 2.10 A 83-231 [» ]
    1A3U X-ray 2.05 A 83-231 [» ]
    1A3V X-ray 2.20 A 83-231 [» ]
    1AEX X-ray 2.10 A 83-231 [» ]
    1ENA X-ray 2.15 A 89-223 [» ]
    1ENC X-ray 1.95 A 83-231 [» ]
    1EQV X-ray 1.94 A 88-223 [» ]
    1EY0 X-ray 1.60 A 83-231 [» ]
    1EY4 X-ray 1.60 A 83-231 [» ]
    1EY5 X-ray 1.70 A 83-231 [» ]
    1EY6 X-ray 1.75 A 83-231 [» ]
    1EY7 X-ray 1.88 A 83-231 [» ]
    1EY8 X-ray 1.75 A 83-231 [» ]
    1EY9 X-ray 1.72 A 83-231 [» ]
    1EYA X-ray 2.00 A 83-231 [» ]
    1EYC X-ray 1.85 A 83-231 [» ]
    1EYD X-ray 1.70 A 83-231 [» ]
    1EZ6 X-ray 1.90 A 83-231 [» ]
    1EZ8 X-ray 1.85 A 83-231 [» ]
    1F2M X-ray 2.00 A 83-231 [» ]
    1F2Y X-ray 2.00 A 83-231 [» ]
    1F2Z X-ray 1.80 A 83-231 [» ]
    1IHZ X-ray 1.65 A 83-231 [» ]
    1II3 X-ray 1.72 A 83-231 [» ]
    1JOK NMR - A 83-231 [» ]
    1JOO NMR - A 83-231 [» ]
    1JOQ NMR - A 83-231 [» ]
    1JOR NMR - A 83-231 [» ]
    1KAA X-ray 1.90 A 88-223 [» ]
    1KAB X-ray 1.80 A 88-223 [» ]
    1KDA X-ray 1.90 A 83-231 [» ]
    1KDB X-ray 1.90 A 83-231 [» ]
    1KDC X-ray 2.00 A 83-231 [» ]
    1NSN X-ray 2.80 S 83-231 [» ]
    1NUC X-ray 1.90 A 83-231 [» ]
    1RKN NMR - A 83-192 [» ]
    1SNC X-ray 1.65 A 83-231 [» ]
    1SND X-ray 1.84 A/B 83-231 [» ]
    1SNM X-ray 1.74 A 83-231 [» ]
    1SNO X-ray 1.70 A 83-231 [» ]
    1SNP X-ray 1.95 A 83-231 [» ]
    1SNQ X-ray 1.70 A 83-231 [» ]
    1STA X-ray 1.70 A 83-229 [» ]
    1STB X-ray 2.00 A 83-230 [» ]
    1STG X-ray 1.70 A 83-231 [» ]
    1STH X-ray 1.85 A 83-231 [» ]
    1STN X-ray 1.70 A 83-231 [» ]
    1STY X-ray 1.67 A 83-230 [» ]
    1SYB X-ray 1.80 A 83-231 [» ]
    1SYC X-ray 1.80 A 83-231 [» ]
    1SYD X-ray 1.70 A 83-231 [» ]
    1SYE X-ray 1.80 A 83-231 [» ]
    1SYF X-ray 1.80 A 83-231 [» ]
    1SYG X-ray 1.90 A 83-231 [» ]
    1TQO X-ray 2.00 A 83-226 [» ]
    1TR5 X-ray 2.10 A 83-226 [» ]
    1TT2 X-ray 1.85 A 83-226 [» ]
    1U9R X-ray 2.10 A 83-231 [» ]
    2ENB X-ray 2.05 A 89-223 [» ]
    2EXZ X-ray 1.90 A 83-231 [» ]
    2EY1 X-ray 1.85 A 83-231 [» ]
    2EY2 X-ray 1.70 A 83-231 [» ]
    2EY5 X-ray 2.00 A 83-231 [» ]
    2EY6 X-ray 1.65 A 83-231 [» ]
    2EYF X-ray 1.80 A 83-231 [» ]
    2EYH X-ray 1.90 A 83-231 [» ]
    2EYJ X-ray 1.85 A 83-231 [» ]
    2EYL X-ray 1.70 A 83-231 [» ]
    2EYM X-ray 1.80 A 83-231 [» ]
    2EYO X-ray 1.70 A 83-231 [» ]
    2EYP X-ray 1.80 A 83-231 [» ]
    2F0D X-ray 2.50 A 83-231 [» ]
    2F0E X-ray 1.95 A 83-231 [» ]
    2F0F X-ray 2.15 A 83-231 [» ]
    2F0G X-ray 1.90 A 83-231 [» ]
    2F0H X-ray 2.15 A 83-231 [» ]
    2F0I X-ray 1.80 A 83-231 [» ]
    2F0J X-ray 1.80 A 83-231 [» ]
    2F0K X-ray 1.90 A 83-231 [» ]
    2F0L X-ray 1.90 A 83-231 [» ]
    2F0M X-ray 2.00 A 83-231 [» ]
    2F0N X-ray 1.70 A 83-231 [» ]
    2F0O X-ray 1.90 A 83-231 [» ]
    2F0P X-ray 2.05 A 83-231 [» ]
    2F0Q X-ray 1.95 A 83-231 [» ]
    2F0S X-ray 1.75 A 83-231 [» ]
    2F0T X-ray 1.70 A 83-231 [» ]
    2F0U X-ray 1.83 A 83-231 [» ]
    2F0V X-ray 2.00 A 83-231 [» ]
    2F0W X-ray 2.10 A 83-231 [» ]
    2F3V NMR - A 83-192 [» ]
    2F3W NMR - A 83-192 [» ]
    2FXY NMR - A 137-154 [» ]
    2FXZ NMR - A 179-191 [» ]
    2GSI X-ray 2.81 W/X/Y/Z 126-136 [» ]
    2KHS NMR - A 83-203 [» ]
    2NUC X-ray 2.00 A 83-231 [» ]
    2OEO X-ray 2.00 A 83-226 [» ]
    2OF1 X-ray 1.92 A 83-226 [» ]
    2PQE NMR - A 83-231 [» ]
    2QDB X-ray 2.20 A 83-231 [» ]
    2RBM X-ray 1.90 A 83-231 [» ]
    2RDF X-ray 2.01 A 83-231 [» ]
    2RKS X-ray 2.01 A 83-231 [» ]
    2SNM X-ray 1.97 A 83-231 [» ]
    2SNS X-ray 1.50 A 83-231 [» ]
    2SOB NMR - A 83-185 [» ]
    3BDC X-ray 1.80 A 83-231 [» ]
    3C1E X-ray 1.90 A 83-231 [» ]
    3C1F X-ray 2.00 A 83-231 [» ]
    3D4D X-ray 2.10 A/B 83-231 [» ]
    3D6C X-ray 2.00 A 83-231 [» ]
    3DHQ X-ray 2.15 A 83-231 [» ]
    3DMU X-ray 1.80 A 83-231 [» ]
    3E5S X-ray 2.00 A/B 83-231 [» ]
    3EJI X-ray 1.90 A 83-231 [» ]
    3ERO X-ray 1.85 A 83-231 [» ]
    3ERQ X-ray 2.10 A 83-231 [» ]
    3EVQ X-ray 2.00 A 83-231 [» ]
    3H6M X-ray 1.70 A 83-231 [» ]
    3HEJ X-ray 1.80 A/B/C/D 83-231 [» ]
    3HZX X-ray 2.00 A 83-231 [» ]
    3ITP X-ray 1.75 A 83-231 [» ]
    3LX0 X-ray 1.50 A 83-231 [» ]
    3MEH X-ray 1.50 A 83-231 [» ]
    3MHB X-ray 1.55 A 83-231 [» ]
    3MXP X-ray 1.61 A 83-231 [» ]
    3MZ5 X-ray 1.58 A 83-231 [» ]
    3NHH X-ray 2.00 A 83-231 [» ]
    3NK9 X-ray 1.65 A 83-231 [» ]
    3NP8 X-ray 1.70 A 83-231 [» ]
    3NQT X-ray 1.90 A 83-231 [» ]
    3NUC X-ray 1.90 A 83-231 [» ]
    3NXW X-ray 1.65 A 83-231 [» ]
    3OSO X-ray 1.60 A 83-231 [» ]
    3OWF X-ray 1.85 A 83-231 [» ]
    3P1H X-ray 1.79 A 83-231 [» ]
    3P75 X-ray 1.90 A 89-223 [» ]
    3PMF X-ray 1.60 A 83-231 [» ]
    3QB3 X-ray 1.63 A 83-231 [» ]
    3RUZ X-ray 1.58 A 83-231 [» ]
    3S9W X-ray 1.90 A 83-231 [» ]
    3SHL X-ray 1.48 A 83-231 [» ]
    3SK4 X-ray 1.85 A 83-231 [» ]
    3SK5 X-ray 1.70 A 83-231 [» ]
    3SK6 X-ray 1.80 A 83-231 [» ]
    3SK8 X-ray 1.90 A 83-231 [» ]
    3SR1 X-ray 1.45 A 83-231 [» ]
    3SXH X-ray 1.40 A 83-231 [» ]
    3T13 X-ray 1.80 A/B 83-231 [» ]
    3T16 X-ray 1.80 A 83-231 [» ]
    3TME X-ray 1.40 A 83-231 [» ]
    3TP5 X-ray 1.80 A 83-231 [» ]
    3TP6 X-ray 1.80 A 83-231 [» ]
    3TP7 X-ray 1.90 A 83-231 [» ]
    3TP8 X-ray 1.60 A 83-231 [» ]
    3U9O X-ray 1.95 A 83-231 [» ]
    3V2T X-ray 1.80 A 83-231 [» ]
    3VA5 X-ray 1.55 A 83-231 [» ]
    4DF7 X-ray 1.50 A 83-231 [» ]
    4DFA X-ray 1.40 A 83-231 [» ]
    4DGZ X-ray 1.47 A 83-231 [» ]
    4DU9 X-ray 1.63 A 83-231 [» ]
    4E6I X-ray 2.00 A/B 83-231 [» ]
    4EQN X-ray 1.80 A 83-231 [» ]
    4EQO X-ray 1.70 A 83-231 [» ]
    4EQP X-ray 1.35 A 83-231 [» ]
    4EVO X-ray 1.95 A 83-231 [» ]
    4F7X X-ray 1.70 A 83-231 [» ]
    4F8M X-ray 1.60 A 83-231 [» ]
    4G57 X-ray 1.81 A 89-223 [» ]
    4H7B X-ray 1.60 A 83-231 [» ]
    4HMI X-ray 1.75 A 83-231 [» ]
    4HMJ X-ray 1.35 A 83-231 [» ]
    4HTH X-ray 1.75 A 83-231 [» ]
    4I65 X-ray 1.61 A 88-223 [» ]
    4IAL X-ray 1.60 A 83-231 [» ]
    4ID6 X-ray 1.90 A 83-231 [» ]
    4IUN X-ray 1.60 A 83-231 [» ]
    4IZ8 X-ray 1.70 A 83-231 [» ]
    4J1M X-ray 1.65 A 83-231 [» ]
    4J6H X-ray 1.60 A 83-231 [» ]
    4K14 X-ray 1.60 A 88-223 [» ]
    4K2K X-ray 1.65 A 83-231 [» ]
    4K2L X-ray 1.55 A 83-231 [» ]
    4K5V X-ray 1.75 A 83-231 [» ]
    4K5W X-ray 1.65 A 83-231 [» ]
    4K5X X-ray 1.65 A 83-231 [» ]
    4K6D X-ray 1.65 A 83-231 [» ]
    4K8I X-ray 2.10 A 89-223 [» ]
    4K8J X-ray 2.00 A 89-223 [» ]
    4KD3 X-ray 1.75 A 83-231 [» ]
    4KD4 X-ray 1.55 A 83-231 [» ]
    4KHV X-ray 1.60 A 83-231 [» ]
    4KJN X-ray 1.55 A 83-231 [» ]
    4KJO X-ray 1.90 A 83-231 [» ]
    4KTA X-ray 1.95 A 83-231 [» ]
    4KV6 X-ray 1.55 A 83-231 [» ]
    4KY5 X-ray 1.40 A 83-231 [» ]
    4KY6 X-ray 1.70 A 83-231 [» ]
    4KY7 X-ray 1.55 A 83-231 [» ]
    4LAA X-ray 1.58 A 83-231 [» ]
    4ME5 X-ray 1.80 A 83-231 [» ]
    4MIU X-ray 1.67 A 83-231 [» ]
    4N9P X-ray 1.40 A 83-231 [» ]
    4N9T X-ray 1.60 A 83-231 [» ]
    4NDX X-ray 1.50 A 83-231 [» ]
    4NKL X-ray 1.60 A 83-231 [» ]
    4NMZ X-ray 1.70 A 83-231 [» ]
    4NP5 X-ray 1.50 A 83-231 [» ]
    4ODG X-ray 1.73 A 83-231 [» ]
    4PNY X-ray 1.68 A 83-231 [» ]
    5NUC X-ray 2.10 A 83-231 [» ]
    ProteinModelPortali P00644.
    SMRi P00644. Positions 86-223.
    ModBasei Search...

    Chemistry

    DrugBanki DB01093. Dimethyl sulfoxide.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG1525.

    Miscellaneous databases

    EvolutionaryTracei P00644.

    Family and domain databases

    Gene3Di 2.40.50.90. 1 hit.
    InterProi IPR016071. Staphylococal_nuclease_OB-fold.
    IPR002071. Thermonucl_AS.
    [Graphical view ]
    Pfami PF00565. SNase. 1 hit.
    [Graphical view ]
    SMARTi SM00318. SNc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50199. SSF50199. 1 hit.
    PROSITEi PS01123. TNASE_1. 1 hit.
    PS01284. TNASE_2. 1 hit.
    PS50830. TNASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A genetic system for analysis of staphylococcal nuclease."
      Shortle D.
      Gene 22:181-189(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 27735 / Foggie.
    2. "Secretion of staphylococcal nuclease by Bacillus subtilis."
      Kovacevic S., Veal L.E., Hsiung H.M., Miller J.R.
      J. Bacteriol. 162:521-528(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
    3. "Secretion and processing of staphylococcal nuclease by Bacillus subtilis."
      Miller J.R., Kovacevic S., Veal L.E.
      J. Bacteriol. 169:3508-3514(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-63.
    4. "Nuclease B. A possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus."
      Davis A., Moore I.B., Parker D.S., Taniuchi H.
      J. Biol. Chem. 252:6544-6553(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 64-82.
      Strain: ATCC 27735 / Foggie.
    5. "Staphylococcal nuclease (Foggi strain). II. The amino acid sequence."
      Cone J.L., Cusumano C.L., Taniuchi H., Anfinsen C.B.
      J. Biol. Chem. 246:3103-3110(1971) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 83-231.
      Strain: ATCC 27735 / Foggie.
    6. "The examination of the presence of amide groups in glutamic acid and aspartic acid residues of staphylococcal nuclease (Foggi strain)."
      Bohnert J.L., Taniuchi H.
      J. Biol. Chem. 247:4557-4560(1972) [PubMed] [Europe PMC] [Abstract]
      Cited for: AMIDE ASSIGNMENTS FOR 83-231.
      Strain: ATCC 27735 / Foggie.
    7. "Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution."
      Cotton F.A., Hazen E.C. Jr., Legg M.J.
      Proc. Natl. Acad. Sci. U.S.A. 76:2551-2555(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), PROPOSED CATALYTIC MECHANISM.
    8. "The crystal structure of staphylococcal nuclease refined at 1.7-A resolution."
      Hynes T.R., Fox R.O.
      Proteins 10:92-105(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
    9. "Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N resonances and secondary structure of the unligated enzyme as identified by three-dimensional NMR spectroscopy."
      Wang J., Mooberry E.S., Walkenhorst W.F., Markley J.L.
      Biochemistry 31:911-920(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    10. "NMR analysis of the residual structure in the denatured state of an unusual mutant of staphylococcal nuclease."
      Shortle D., Abeygunawardana C.
      Structure 1:121-134(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 83-218.

    Entry informationi

    Entry nameiNUC_STAAU
    AccessioniPrimary (citable) accession number: P00644
    Secondary accession number(s): P13718, Q53711
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: May 14, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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