Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Calcium
Active sitei117 – 11711 Publication
Metal bindingi122 – 1221Calcium
Metal bindingi123 – 1231Calcium; via carbonyl oxygen
Active sitei125 – 12511 Publication
Active sitei169 – 16911 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.31.1. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Cleaved into the following 2 chains:
Gene namesi
Name:nuc
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Propeptidei27 – 63371 PublicationPRO_0000045226Add
BLAST
Chaini64 – 231168Nuclease BPRO_0000034389Add
BLAST
Propeptidei64 – 82191 PublicationPRO_0000034390Add
BLAST
Chaini83 – 231149Nuclease APRO_0000034391Add
BLAST

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi93062.SACOL0860.

Structurei

Secondary structure

1
231
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni84 – 863Combined sources
Beta strandi91 – 999Combined sources
Beta strandi101 – 1099Combined sources
Beta strandi112 – 1187Combined sources
Beta strandi120 – 1223Combined sources
Beta strandi126 – 1283Combined sources
Beta strandi129 – 1313Combined sources
Beta strandi134 – 1363Combined sources
Helixi137 – 15014Combined sources
Beta strandi154 – 1574Combined sources
Beta strandi166 – 1683Combined sources
Beta strandi170 – 1767Combined sources
Beta strandi177 – 1793Combined sources
Helixi181 – 1877Combined sources
Beta strandi190 – 1934Combined sources
Beta strandi198 – 2003Combined sources
Turni201 – 2033Combined sources
Helixi204 – 21613Combined sources
Helixi220 – 2223Combined sources
Beta strandi227 – 2293Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2TX-ray1.96A83-231[»]
1A2UX-ray2.00A83-231[»]
1A3TX-ray2.10A83-231[»]
1A3UX-ray2.05A83-231[»]
1A3VX-ray2.20A83-231[»]
1AEXX-ray2.10A83-231[»]
1ENAX-ray2.15A89-223[»]
1ENCX-ray1.95A83-231[»]
1EQVX-ray1.94A88-223[»]
1EY0X-ray1.60A83-231[»]
1EY4X-ray1.60A83-231[»]
1EY5X-ray1.70A83-231[»]
1EY6X-ray1.75A83-231[»]
1EY7X-ray1.88A83-231[»]
1EY8X-ray1.75A83-231[»]
1EY9X-ray1.72A83-231[»]
1EYAX-ray2.00A83-231[»]
1EYCX-ray1.85A83-231[»]
1EYDX-ray1.70A83-231[»]
1EZ6X-ray1.90A83-231[»]
1EZ8X-ray1.85A83-231[»]
1F2MX-ray2.00A83-231[»]
1F2YX-ray2.00A83-231[»]
1F2ZX-ray1.80A83-231[»]
1IHZX-ray1.65A83-231[»]
1II3X-ray1.72A83-231[»]
1JOKNMR-A83-231[»]
1JOONMR-A83-231[»]
1JOQNMR-A83-231[»]
1JORNMR-A83-231[»]
1KAAX-ray1.90A88-223[»]
1KABX-ray1.80A88-223[»]
1KDAX-ray1.90A83-231[»]
1KDBX-ray1.90A83-231[»]
1KDCX-ray2.00A83-231[»]
1NSNX-ray2.80S83-231[»]
1NUCX-ray1.90A83-231[»]
1RKNNMR-A83-192[»]
1SNCX-ray1.65A83-231[»]
1SNDX-ray1.84A/B83-231[»]
1SNMX-ray1.74A83-231[»]
1SNOX-ray1.70A83-231[»]
1SNPX-ray1.95A83-231[»]
1SNQX-ray1.70A83-231[»]
1STAX-ray1.70A83-229[»]
1STBX-ray2.00A83-230[»]
1STGX-ray1.70A83-231[»]
1STHX-ray1.85A83-231[»]
1STNX-ray1.70A83-231[»]
1STYX-ray1.67A83-230[»]
1SYBX-ray1.80A83-231[»]
1SYCX-ray1.80A83-231[»]
1SYDX-ray1.70A83-231[»]
1SYEX-ray1.80A83-231[»]
1SYFX-ray1.80A83-231[»]
1SYGX-ray1.90A83-231[»]
1TQOX-ray2.00A83-226[»]
1TR5X-ray2.10A83-226[»]
1TT2X-ray1.85A83-226[»]
1U9RX-ray2.10A83-231[»]
2ENBX-ray2.05A89-223[»]
2EXZX-ray1.90A83-231[»]
2EY1X-ray1.85A83-231[»]
2EY2X-ray1.70A83-231[»]
2EY5X-ray2.00A83-231[»]
2EY6X-ray1.65A83-231[»]
2EYFX-ray1.80A83-231[»]
2EYHX-ray1.90A83-231[»]
2EYJX-ray1.85A83-231[»]
2EYLX-ray1.70A83-231[»]
2EYMX-ray1.80A83-231[»]
2EYOX-ray1.70A83-231[»]
2EYPX-ray1.80A83-231[»]
2F0DX-ray2.50A83-231[»]
2F0EX-ray1.95A83-231[»]
2F0FX-ray2.15A83-231[»]
2F0GX-ray1.90A83-231[»]
2F0HX-ray2.15A83-231[»]
2F0IX-ray1.80A83-231[»]
2F0JX-ray1.80A83-231[»]
2F0KX-ray1.90A83-231[»]
2F0LX-ray1.90A83-231[»]
2F0MX-ray2.00A83-231[»]
2F0NX-ray1.70A83-231[»]
2F0OX-ray1.90A83-231[»]
2F0PX-ray2.05A83-231[»]
2F0QX-ray1.95A83-231[»]
2F0SX-ray1.75A83-231[»]
2F0TX-ray1.70A83-231[»]
2F0UX-ray1.83A83-231[»]
2F0VX-ray2.00A83-231[»]
2F0WX-ray2.10A83-231[»]
2F3VNMR-A83-192[»]
2F3WNMR-A83-192[»]
2FXYNMR-A137-154[»]
2FXZNMR-A179-191[»]
2GSIX-ray2.81W/X/Y/Z126-136[»]
2KHSNMR-A83-203[»]
2NUCX-ray2.00A83-231[»]
2OEOX-ray2.00A83-226[»]
2OF1X-ray1.92A83-226[»]
2PQENMR-A83-231[»]
2QDBX-ray2.20A83-231[»]
2RBMX-ray1.90A83-231[»]
2RDFX-ray2.01A83-231[»]
2RKSX-ray2.01A83-231[»]
2SNMX-ray1.97A83-231[»]
2SNSX-ray1.50A83-231[»]
2SOBNMR-A83-185[»]
3BDCX-ray1.80A83-231[»]
3C1EX-ray1.90A83-231[»]
3C1FX-ray2.00A83-231[»]
3D4DX-ray2.10A/B83-231[»]
3D6CX-ray2.00A83-231[»]
3DHQX-ray2.15A83-231[»]
3DMUX-ray1.80A83-231[»]
3E5SX-ray2.00A/B83-231[»]
3EJIX-ray1.90A83-231[»]
3EROX-ray1.85A83-231[»]
3ERQX-ray2.10A83-231[»]
3EVQX-ray2.00A83-231[»]
3H6MX-ray1.70A83-231[»]
3HEJX-ray1.80A/B/C/D83-231[»]
3HZXX-ray2.00A83-231[»]
3ITPX-ray1.75A83-231[»]
3LX0X-ray1.50A83-231[»]
3MEHX-ray1.50A83-231[»]
3MHBX-ray1.55A83-231[»]
3MXPX-ray1.61A83-231[»]
3MZ5X-ray1.58A83-231[»]
3NHHX-ray2.00A83-231[»]
3NK9X-ray1.65A83-231[»]
3NP8X-ray1.70A83-231[»]
3NQTX-ray1.90A83-231[»]
3NUCX-ray1.90A83-231[»]
3NXWX-ray1.65A83-231[»]
3OSOX-ray1.60A83-231[»]
3OWFX-ray1.85A83-231[»]
3P1HX-ray1.79A83-231[»]
3P75X-ray1.90A89-223[»]
3PMFX-ray1.60A83-231[»]
3QB3X-ray1.63A83-231[»]
3RUZX-ray1.58A83-231[»]
3S9WX-ray1.90A83-231[»]
3SHLX-ray1.48A83-231[»]
3SK4X-ray1.85A83-231[»]
3SK5X-ray1.70A83-231[»]
3SK6X-ray1.80A83-231[»]
3SK8X-ray1.90A83-231[»]
3SR1X-ray1.45A83-231[»]
3SXHX-ray1.40A83-231[»]
3T13X-ray1.80A/B83-231[»]
3T16X-ray1.80A83-231[»]
3TMEX-ray1.40A83-231[»]
3TP5X-ray1.80A83-231[»]
3TP6X-ray1.80A83-231[»]
3TP7X-ray1.90A83-231[»]
3TP8X-ray1.60A83-231[»]
3U9OX-ray1.95A83-231[»]
3V2TX-ray1.80A83-231[»]
3VA5X-ray1.55A83-231[»]
4DF7X-ray1.50A83-231[»]
4DFAX-ray1.40A83-231[»]
4DGZX-ray1.47A83-231[»]
4DU9X-ray1.63A83-231[»]
4E6IX-ray2.00A/B83-231[»]
4EQNX-ray1.80A83-231[»]
4EQOX-ray1.70A83-231[»]
4EQPX-ray1.35A83-231[»]
4EVOX-ray1.95A83-231[»]
4F7XX-ray1.70A83-231[»]
4F8MX-ray1.60A83-231[»]
4G57X-ray1.81A89-223[»]
4H7BX-ray1.60A83-231[»]
4HMIX-ray1.75A83-231[»]
4HMJX-ray1.35A83-231[»]
4HTHX-ray1.75A83-231[»]
4I65X-ray1.61A88-223[»]
4IALX-ray1.60A83-231[»]
4ID6X-ray1.90A83-231[»]
4IUNX-ray1.60A83-231[»]
4IZ8X-ray1.70A83-231[»]
4J1MX-ray1.65A83-231[»]
4J6HX-ray1.60A83-231[»]
4K14X-ray1.60A88-223[»]
4K2KX-ray1.65A83-231[»]
4K2LX-ray1.55A83-231[»]
4K5VX-ray1.75A83-231[»]
4K5WX-ray1.65A83-231[»]
4K5XX-ray1.65A83-231[»]
4K6DX-ray1.65A83-231[»]
4K8IX-ray2.10A89-223[»]
4K8JX-ray2.00A89-223[»]
4KD3X-ray1.75A83-231[»]
4KD4X-ray1.55A83-231[»]
4KHVX-ray1.60A83-231[»]
4KJNX-ray1.55A83-231[»]
4KJOX-ray1.90A83-231[»]
4KTAX-ray1.95A83-231[»]
4KV6X-ray1.55A83-231[»]
4KY5X-ray1.40A83-231[»]
4KY6X-ray1.70A83-231[»]
4KY7X-ray1.55A83-231[»]
4LAAX-ray1.58A83-231[»]
4ME5X-ray1.80A83-231[»]
4MIUX-ray1.67A83-231[»]
4N9PX-ray1.40A83-231[»]
4N9TX-ray1.60A83-231[»]
4NDXX-ray1.50A83-231[»]
4NKLX-ray1.60A83-231[»]
4NMZX-ray1.70A83-231[»]
4NP5X-ray1.50A83-231[»]
4ODGX-ray1.73A83-231[»]
4OL7X-ray1.67A/B83-231[»]
4PMBX-ray1.80A83-231[»]
4PMCX-ray1.65A83-231[»]
4PNYX-ray1.68A83-231[»]
4QB4X-ray1.60A88-223[»]
4QF4X-ray1.80A/B83-231[»]
4R8NX-ray1.65A83-223[»]
4RKBX-ray1.88A83-231[»]
4RKLX-ray1.66A83-231[»]
4TRDX-ray1.60A83-231[»]
4WORX-ray1.60A83-231[»]
4YIJX-ray1.64A/B83-231[»]
5NUCX-ray2.10A83-231[»]
ProteinModelPortaliP00644.
SMRiP00644. Positions 86-223.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00644.

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1525.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT
60 70 80 90 100
LVVVSSLSSS ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI
110 120 130 140 150
DGDTVKLMYK GQPMTFRLLL VDTPETKHPK KGVEKYGPEA SAFTKKMVEN
160 170 180 190 200
AKKIEVEFDK GQRTDKYGRG LAYIYADGKM VNEALVRQGL AKVAYVYKPN
210 220 230
NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q
Length:231
Mass (Da):25,471
Last modified:July 21, 1986 - v1
Checksum:iAD5558AA28E855D7
GO

Sequence cautioni

The sequence AAA26659.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01281 mRNA. Translation: CAA24594.1.
M17123 Genomic DNA. Translation: AAA26659.1. Different initiation.
PIRiA00790. NCSAF.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01281 mRNA. Translation: CAA24594.1.
M17123 Genomic DNA. Translation: AAA26659.1. Different initiation.
PIRiA00790. NCSAF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2TX-ray1.96A83-231[»]
1A2UX-ray2.00A83-231[»]
1A3TX-ray2.10A83-231[»]
1A3UX-ray2.05A83-231[»]
1A3VX-ray2.20A83-231[»]
1AEXX-ray2.10A83-231[»]
1ENAX-ray2.15A89-223[»]
1ENCX-ray1.95A83-231[»]
1EQVX-ray1.94A88-223[»]
1EY0X-ray1.60A83-231[»]
1EY4X-ray1.60A83-231[»]
1EY5X-ray1.70A83-231[»]
1EY6X-ray1.75A83-231[»]
1EY7X-ray1.88A83-231[»]
1EY8X-ray1.75A83-231[»]
1EY9X-ray1.72A83-231[»]
1EYAX-ray2.00A83-231[»]
1EYCX-ray1.85A83-231[»]
1EYDX-ray1.70A83-231[»]
1EZ6X-ray1.90A83-231[»]
1EZ8X-ray1.85A83-231[»]
1F2MX-ray2.00A83-231[»]
1F2YX-ray2.00A83-231[»]
1F2ZX-ray1.80A83-231[»]
1IHZX-ray1.65A83-231[»]
1II3X-ray1.72A83-231[»]
1JOKNMR-A83-231[»]
1JOONMR-A83-231[»]
1JOQNMR-A83-231[»]
1JORNMR-A83-231[»]
1KAAX-ray1.90A88-223[»]
1KABX-ray1.80A88-223[»]
1KDAX-ray1.90A83-231[»]
1KDBX-ray1.90A83-231[»]
1KDCX-ray2.00A83-231[»]
1NSNX-ray2.80S83-231[»]
1NUCX-ray1.90A83-231[»]
1RKNNMR-A83-192[»]
1SNCX-ray1.65A83-231[»]
1SNDX-ray1.84A/B83-231[»]
1SNMX-ray1.74A83-231[»]
1SNOX-ray1.70A83-231[»]
1SNPX-ray1.95A83-231[»]
1SNQX-ray1.70A83-231[»]
1STAX-ray1.70A83-229[»]
1STBX-ray2.00A83-230[»]
1STGX-ray1.70A83-231[»]
1STHX-ray1.85A83-231[»]
1STNX-ray1.70A83-231[»]
1STYX-ray1.67A83-230[»]
1SYBX-ray1.80A83-231[»]
1SYCX-ray1.80A83-231[»]
1SYDX-ray1.70A83-231[»]
1SYEX-ray1.80A83-231[»]
1SYFX-ray1.80A83-231[»]
1SYGX-ray1.90A83-231[»]
1TQOX-ray2.00A83-226[»]
1TR5X-ray2.10A83-226[»]
1TT2X-ray1.85A83-226[»]
1U9RX-ray2.10A83-231[»]
2ENBX-ray2.05A89-223[»]
2EXZX-ray1.90A83-231[»]
2EY1X-ray1.85A83-231[»]
2EY2X-ray1.70A83-231[»]
2EY5X-ray2.00A83-231[»]
2EY6X-ray1.65A83-231[»]
2EYFX-ray1.80A83-231[»]
2EYHX-ray1.90A83-231[»]
2EYJX-ray1.85A83-231[»]
2EYLX-ray1.70A83-231[»]
2EYMX-ray1.80A83-231[»]
2EYOX-ray1.70A83-231[»]
2EYPX-ray1.80A83-231[»]
2F0DX-ray2.50A83-231[»]
2F0EX-ray1.95A83-231[»]
2F0FX-ray2.15A83-231[»]
2F0GX-ray1.90A83-231[»]
2F0HX-ray2.15A83-231[»]
2F0IX-ray1.80A83-231[»]
2F0JX-ray1.80A83-231[»]
2F0KX-ray1.90A83-231[»]
2F0LX-ray1.90A83-231[»]
2F0MX-ray2.00A83-231[»]
2F0NX-ray1.70A83-231[»]
2F0OX-ray1.90A83-231[»]
2F0PX-ray2.05A83-231[»]
2F0QX-ray1.95A83-231[»]
2F0SX-ray1.75A83-231[»]
2F0TX-ray1.70A83-231[»]
2F0UX-ray1.83A83-231[»]
2F0VX-ray2.00A83-231[»]
2F0WX-ray2.10A83-231[»]
2F3VNMR-A83-192[»]
2F3WNMR-A83-192[»]
2FXYNMR-A137-154[»]
2FXZNMR-A179-191[»]
2GSIX-ray2.81W/X/Y/Z126-136[»]
2KHSNMR-A83-203[»]
2NUCX-ray2.00A83-231[»]
2OEOX-ray2.00A83-226[»]
2OF1X-ray1.92A83-226[»]
2PQENMR-A83-231[»]
2QDBX-ray2.20A83-231[»]
2RBMX-ray1.90A83-231[»]
2RDFX-ray2.01A83-231[»]
2RKSX-ray2.01A83-231[»]
2SNMX-ray1.97A83-231[»]
2SNSX-ray1.50A83-231[»]
2SOBNMR-A83-185[»]
3BDCX-ray1.80A83-231[»]
3C1EX-ray1.90A83-231[»]
3C1FX-ray2.00A83-231[»]
3D4DX-ray2.10A/B83-231[»]
3D6CX-ray2.00A83-231[»]
3DHQX-ray2.15A83-231[»]
3DMUX-ray1.80A83-231[»]
3E5SX-ray2.00A/B83-231[»]
3EJIX-ray1.90A83-231[»]
3EROX-ray1.85A83-231[»]
3ERQX-ray2.10A83-231[»]
3EVQX-ray2.00A83-231[»]
3H6MX-ray1.70A83-231[»]
3HEJX-ray1.80A/B/C/D83-231[»]
3HZXX-ray2.00A83-231[»]
3ITPX-ray1.75A83-231[»]
3LX0X-ray1.50A83-231[»]
3MEHX-ray1.50A83-231[»]
3MHBX-ray1.55A83-231[»]
3MXPX-ray1.61A83-231[»]
3MZ5X-ray1.58A83-231[»]
3NHHX-ray2.00A83-231[»]
3NK9X-ray1.65A83-231[»]
3NP8X-ray1.70A83-231[»]
3NQTX-ray1.90A83-231[»]
3NUCX-ray1.90A83-231[»]
3NXWX-ray1.65A83-231[»]
3OSOX-ray1.60A83-231[»]
3OWFX-ray1.85A83-231[»]
3P1HX-ray1.79A83-231[»]
3P75X-ray1.90A89-223[»]
3PMFX-ray1.60A83-231[»]
3QB3X-ray1.63A83-231[»]
3RUZX-ray1.58A83-231[»]
3S9WX-ray1.90A83-231[»]
3SHLX-ray1.48A83-231[»]
3SK4X-ray1.85A83-231[»]
3SK5X-ray1.70A83-231[»]
3SK6X-ray1.80A83-231[»]
3SK8X-ray1.90A83-231[»]
3SR1X-ray1.45A83-231[»]
3SXHX-ray1.40A83-231[»]
3T13X-ray1.80A/B83-231[»]
3T16X-ray1.80A83-231[»]
3TMEX-ray1.40A83-231[»]
3TP5X-ray1.80A83-231[»]
3TP6X-ray1.80A83-231[»]
3TP7X-ray1.90A83-231[»]
3TP8X-ray1.60A83-231[»]
3U9OX-ray1.95A83-231[»]
3V2TX-ray1.80A83-231[»]
3VA5X-ray1.55A83-231[»]
4DF7X-ray1.50A83-231[»]
4DFAX-ray1.40A83-231[»]
4DGZX-ray1.47A83-231[»]
4DU9X-ray1.63A83-231[»]
4E6IX-ray2.00A/B83-231[»]
4EQNX-ray1.80A83-231[»]
4EQOX-ray1.70A83-231[»]
4EQPX-ray1.35A83-231[»]
4EVOX-ray1.95A83-231[»]
4F7XX-ray1.70A83-231[»]
4F8MX-ray1.60A83-231[»]
4G57X-ray1.81A89-223[»]
4H7BX-ray1.60A83-231[»]
4HMIX-ray1.75A83-231[»]
4HMJX-ray1.35A83-231[»]
4HTHX-ray1.75A83-231[»]
4I65X-ray1.61A88-223[»]
4IALX-ray1.60A83-231[»]
4ID6X-ray1.90A83-231[»]
4IUNX-ray1.60A83-231[»]
4IZ8X-ray1.70A83-231[»]
4J1MX-ray1.65A83-231[»]
4J6HX-ray1.60A83-231[»]
4K14X-ray1.60A88-223[»]
4K2KX-ray1.65A83-231[»]
4K2LX-ray1.55A83-231[»]
4K5VX-ray1.75A83-231[»]
4K5WX-ray1.65A83-231[»]
4K5XX-ray1.65A83-231[»]
4K6DX-ray1.65A83-231[»]
4K8IX-ray2.10A89-223[»]
4K8JX-ray2.00A89-223[»]
4KD3X-ray1.75A83-231[»]
4KD4X-ray1.55A83-231[»]
4KHVX-ray1.60A83-231[»]
4KJNX-ray1.55A83-231[»]
4KJOX-ray1.90A83-231[»]
4KTAX-ray1.95A83-231[»]
4KV6X-ray1.55A83-231[»]
4KY5X-ray1.40A83-231[»]
4KY6X-ray1.70A83-231[»]
4KY7X-ray1.55A83-231[»]
4LAAX-ray1.58A83-231[»]
4ME5X-ray1.80A83-231[»]
4MIUX-ray1.67A83-231[»]
4N9PX-ray1.40A83-231[»]
4N9TX-ray1.60A83-231[»]
4NDXX-ray1.50A83-231[»]
4NKLX-ray1.60A83-231[»]
4NMZX-ray1.70A83-231[»]
4NP5X-ray1.50A83-231[»]
4ODGX-ray1.73A83-231[»]
4OL7X-ray1.67A/B83-231[»]
4PMBX-ray1.80A83-231[»]
4PMCX-ray1.65A83-231[»]
4PNYX-ray1.68A83-231[»]
4QB4X-ray1.60A88-223[»]
4QF4X-ray1.80A/B83-231[»]
4R8NX-ray1.65A83-223[»]
4RKBX-ray1.88A83-231[»]
4RKLX-ray1.66A83-231[»]
4TRDX-ray1.60A83-231[»]
4WORX-ray1.60A83-231[»]
4YIJX-ray1.64A/B83-231[»]
5NUCX-ray2.10A83-231[»]
ProteinModelPortaliP00644.
SMRiP00644. Positions 86-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG1525.

Enzyme and pathway databases

BRENDAi3.1.31.1. 3352.

Miscellaneous databases

EvolutionaryTraceiP00644.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A genetic system for analysis of staphylococcal nuclease."
    Shortle D.
    Gene 22:181-189(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27735 / Foggie.
  2. "Secretion of staphylococcal nuclease by Bacillus subtilis."
    Kovacevic S., Veal L.E., Hsiung H.M., Miller J.R.
    J. Bacteriol. 162:521-528(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
  3. "Secretion and processing of staphylococcal nuclease by Bacillus subtilis."
    Miller J.R., Kovacevic S., Veal L.E.
    J. Bacteriol. 169:3508-3514(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-63.
  4. "Nuclease B. A possible precursor of nuclease A, an extracellular nuclease of Staphylococcus aureus."
    Davis A., Moore I.B., Parker D.S., Taniuchi H.
    J. Biol. Chem. 252:6544-6553(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 64-82.
    Strain: ATCC 27735 / Foggie.
  5. "Staphylococcal nuclease (Foggi strain). II. The amino acid sequence."
    Cone J.L., Cusumano C.L., Taniuchi H., Anfinsen C.B.
    J. Biol. Chem. 246:3103-3110(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 83-231.
    Strain: ATCC 27735 / Foggie.
  6. "The examination of the presence of amide groups in glutamic acid and aspartic acid residues of staphylococcal nuclease (Foggi strain)."
    Bohnert J.L., Taniuchi H.
    J. Biol. Chem. 247:4557-4560(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: AMIDE ASSIGNMENTS FOR 83-231.
    Strain: ATCC 27735 / Foggie.
  7. "Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution."
    Cotton F.A., Hazen E.C. Jr., Legg M.J.
    Proc. Natl. Acad. Sci. U.S.A. 76:2551-2555(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), PROPOSED CATALYTIC MECHANISM.
  8. "The crystal structure of staphylococcal nuclease refined at 1.7-A resolution."
    Hynes T.R., Fox R.O.
    Proteins 10:92-105(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
  9. "Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N resonances and secondary structure of the unligated enzyme as identified by three-dimensional NMR spectroscopy."
    Wang J., Mooberry E.S., Walkenhorst W.F., Markley J.L.
    Biochemistry 31:911-920(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  10. "NMR analysis of the residual structure in the denatured state of an unusual mutant of staphylococcal nuclease."
    Shortle D., Abeygunawardana C.
    Structure 1:121-134(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 83-218.

Entry informationi

Entry nameiNUC_STAAU
AccessioniPrimary (citable) accession number: P00644
Secondary accession number(s): P13718, Q53711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 22, 2015
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.