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P00643 (T2H2_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HhaII

Short name=R.HhaII
EC=3.1.21.4
Alternative name(s):
Endonuclease HhaII
Type II restriction enzyme HhaII
Gene names
Name:hhaIIR
OrganismHaemophilus parahaemolyticus
Taxonomic identifier735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GANTC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

Homodimer.

Caution

Strain ATCC 10014 was originally thought to originate from H.haemolyticus.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Type-2 restriction enzyme HhaII
PRO_0000077318

Sequences

Sequence LengthMass (Da)Tools
P00643 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C3067B0AADA35863

FASTA22725,910
        10         20         30         40         50         60 
MSKISNALGR KDGNSGYTRV VGNAELGQLL SRVQATVISN GNELERLITQ RCNLIENIDV 

        70         80         90        100        110        120 
FIEDTTRGNN VQNGVYLCLK KTFKKSKKYA ESVKGIEPDM LIFIVESYRV CKVIELKDGD 

       130        140        150        160        170        180 
AFDTKKSQGE KEHLEKFATL FGAKIPFVTD YYICSFNQND KKLIMAGFKG VFSLEHILTG 

       190        200        210        220 
KELCQILGIS YQEILDIRRR DTEENFAYLI AEMMKIPEVR EEVKKHF 

« Hide

References

[1]"The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
Schoner B., Kelly S., Smith H.O.
Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
[2]"Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K00508 Genomic DNA. No translation available.
M24624 Genomic DNA. Translation: AAA24964.1.
PIRNDHIH2. A00788.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1118. HhaII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2H2_HAEPH
AccessionPrimary (citable) accession number: P00643
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries