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P00643

- T2H2_HAEPH

UniProt

P00643 - T2H2_HAEPH

Protein

Type-2 restriction enzyme HhaII

Gene

hhaIIR

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5- Protein predictedi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GANTC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1118. HhaII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HhaII (EC:3.1.21.4)
    Short name:
    R.HhaII
    Alternative name(s):
    Endonuclease HhaII
    Type II restriction enzyme HhaII
    Gene namesi
    Name:hhaIIR
    OrganismiHaemophilus parahaemolyticus
    Taxonomic identifieri735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Type-2 restriction enzyme HhaIIPRO_0000077318Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Sequencei

    Sequence statusi: Complete.

    P00643-1 [UniParc]FASTAAdd to Basket

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    MSKISNALGR KDGNSGYTRV VGNAELGQLL SRVQATVISN GNELERLITQ    50
    RCNLIENIDV FIEDTTRGNN VQNGVYLCLK KTFKKSKKYA ESVKGIEPDM 100
    LIFIVESYRV CKVIELKDGD AFDTKKSQGE KEHLEKFATL FGAKIPFVTD 150
    YYICSFNQND KKLIMAGFKG VFSLEHILTG KELCQILGIS YQEILDIRRR 200
    DTEENFAYLI AEMMKIPEVR EEVKKHF 227
    Length:227
    Mass (Da):25,910
    Last modified:July 21, 1986 - v1
    Checksum:iC3067B0AADA35863
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00508 Genomic DNA. No translation available.
    M24624 Genomic DNA. Translation: AAA24964.1.
    PIRiA00788. NDHIH2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00508 Genomic DNA. No translation available.
    M24624 Genomic DNA. Translation: AAA24964.1 .
    PIRi A00788. NDHIH2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1118. HhaII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
      Schoner B., Kelly S., Smith H.O.
      Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
    2. "Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
      Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
      Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
      Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

    Entry informationi

    Entry nameiT2H2_HAEPH
    AccessioniPrimary (citable) accession number: P00643
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Strain ATCC 10014 was originally thought to originate from H.haemolyticus.Curated

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3