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P00643

- T2H2_HAEPH

UniProt

P00643 - T2H2_HAEPH

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Protein

Type-2 restriction enzyme HhaII

Gene

hhaIIR

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GANTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1118. HhaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HhaII (EC:3.1.21.4)
Short name:
R.HhaII
Alternative name(s):
Endonuclease HhaII
Type II restriction enzyme HhaII
Gene namesi
Name:hhaIIR
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Type-2 restriction enzyme HhaIIPRO_0000077318Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Sequencei

Sequence statusi: Complete.

P00643-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSKISNALGR KDGNSGYTRV VGNAELGQLL SRVQATVISN GNELERLITQ
60 70 80 90 100
RCNLIENIDV FIEDTTRGNN VQNGVYLCLK KTFKKSKKYA ESVKGIEPDM
110 120 130 140 150
LIFIVESYRV CKVIELKDGD AFDTKKSQGE KEHLEKFATL FGAKIPFVTD
160 170 180 190 200
YYICSFNQND KKLIMAGFKG VFSLEHILTG KELCQILGIS YQEILDIRRR
210 220
DTEENFAYLI AEMMKIPEVR EEVKKHF
Length:227
Mass (Da):25,910
Last modified:July 21, 1986 - v1
Checksum:iC3067B0AADA35863
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00508 Genomic DNA. No translation available.
M24624 Genomic DNA. Translation: AAA24964.1.
PIRiA00788. NDHIH2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00508 Genomic DNA. No translation available.
M24624 Genomic DNA. Translation: AAA24964.1 .
PIRi A00788. NDHIH2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1118. HhaII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
    Schoner B., Kelly S., Smith H.O.
    Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
  2. "Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
    Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
    Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Entry informationi

Entry nameiT2H2_HAEPH
AccessioniPrimary (citable) accession number: P00643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain ATCC 10014 was originally thought to originate from H.haemolyticus.Curated

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3