P00643 (T2H2_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HhaII
Short name=R.HhaII
EC=3.1.21.4

Alternative name(s):
Endonuclease HhaII
Type II restriction enzyme HhaII

Gene names

Name:

hhaIIR

Organism

Haemophilus parahaemolyticus

Taxonomic identifier

735 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GANTC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	Homodimer.
Caution	Strain ATCC 10014 was originally thought to originate from H.haemolyticus.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Type-2 restriction enzyme HhaII		PRO_0000077318

Sequences

	Sequence		Length	Mass (Da)
	P00643 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C3067B0AADA35863	FASTA	227	25,910
	10 20 30 40 50 60 MSKISNALGR KDGNSGYTRV VGNAELGQLL SRVQATVISN GNELERLITQ RCNLIENIDV 70 80 90 100 110 120 FIEDTTRGNN VQNGVYLCLK KTFKKSKKYA ESVKGIEPDM LIFIVESYRV CKVIELKDGD 130 140 150 160 170 180 AFDTKKSQGE KEHLEKFATL FGAKIPFVTD YYICSFNQND KKLIMAGFKG VFSLEHILTG 190 200 210 220 KELCQILGIS YQEILDIRRR DTEENFAYLI AEMMKIPEVR EEVKKHF

References

[1]	"The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus." Schoner B., Kelly S., Smith H.O. Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
[2]	"Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus." Chandrasegaran S., Wu L.P., Valda E., Smith H.O. Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9. Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K00508 Genomic DNA. No translation available. M24624 Genomic DNA. Translation: AAA24964.1.
PIR	NDHIH2. A00788.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1118. HhaII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2H2_HAEPH

Accession

Primary (citable) accession number: P00643

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program