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Protein

Endonuclease I

Gene

3

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.4 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Bacterial host gene expression shutoff by virus, Degradation of host chromosome by virus, Host gene expression shutoff by virus, Host-virus interaction

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease I (EC:3.1.21.2)
Alternative name(s):
Gene product 3
Short name:
Gp3
Junction-resolving enzyme gp3
Gene namesi
Ordered Locus Names:3
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Endonuclease IPRO_0000106486Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-41668N.
IntActiP00641. 1 interaction.
MINTiMINT-1513471.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 2912Combined sources
Beta strandi35 – 373Combined sources
Beta strandi39 – 457Combined sources
Beta strandi48 – 525Combined sources
Beta strandi55 – 573Combined sources
Beta strandi63 – 697Combined sources
Helixi72 – 8413Combined sources
Beta strandi90 – 956Combined sources
Beta strandi99 – 1024Combined sources
Helixi109 – 1168Combined sources
Beta strandi120 – 1245Combined sources
Helixi127 – 1315Combined sources
Helixi139 – 1413Combined sources
Beta strandi142 – 1443Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZRX-ray2.10A/B/C/D12-149[»]
1M0DX-ray1.90A/B/C/D12-149[»]
1M0IX-ray2.55A/B/C/D12-149[»]
2PFJX-ray3.10A/B1-149[»]
3CAEX-ray3.00A/B/C/D/E/F/G/H/I/J18-145[»]
ProteinModelPortaliP00641.
SMRiP00641. Positions 17-145.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00641.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.30. 1 hit.
InterProiIPR008029. Phage_T7_Gp3_endoDNaseI.
IPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
[Graphical view]
PfamiPF05367. Phage_endo_I. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P00641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGYGAKGIR KVGAFRSGLE DKVSKQLESK GIKFEYEEWK VPYVIPASNH
60 70 80 90 100
TYTPDFLLPN GIFVETKGLW ESDDRKKHLL IREQHPELDI RIVFSSSRTK
110 120 130 140
LYKGSPTSYG EFCEKHGIKF ADKLIPAEWI KEPKKEVPFD RLKRKGGKK
Length:149
Mass (Da):17,172
Last modified:July 21, 1986 - v1
Checksum:iD092AA28E3743BC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01127 Genomic DNA. Translation: CAA24345.1.
V01146 Genomic DNA. Translation: CAA24402.1.
PIRiB94615. NEBP37.
RefSeqiNP_041972.1. NC_001604.1.

Genome annotation databases

GeneIDi1261079.
KEGGivg:1261079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01127 Genomic DNA. Translation: CAA24345.1.
V01146 Genomic DNA. Translation: CAA24402.1.
PIRiB94615. NEBP37.
RefSeqiNP_041972.1. NC_001604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZRX-ray2.10A/B/C/D12-149[»]
1M0DX-ray1.90A/B/C/D12-149[»]
1M0IX-ray2.55A/B/C/D12-149[»]
2PFJX-ray3.10A/B1-149[»]
3CAEX-ray3.00A/B/C/D/E/F/G/H/I/J18-145[»]
ProteinModelPortaliP00641.
SMRiP00641. Positions 17-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41668N.
IntActiP00641. 1 interaction.
MINTiMINT-1513471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261079.
KEGGivg:1261079.

Miscellaneous databases

EvolutionaryTraceiP00641.

Family and domain databases

Gene3Di3.40.91.30. 1 hit.
InterProiIPR008029. Phage_T7_Gp3_endoDNaseI.
IPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
[Graphical view]
PfamiPF05367. Phage_endo_I. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements."
    Dunn J.J., Studier F.W.
    J. Mol. Biol. 166:477-535(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4."
    Dunn J.J., Studier F.W.
    J. Mol. Biol. 148:303-330(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "An endonuclease specific for single-stranded DNA selectively damages the genomic DNA and induces the SOS response."
    Panayotatos N., Fontaine A.
    J. Biol. Chem. 260:3173-3177(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The junction-resolving enzyme T7 endonuclease I: quaternary structure and interaction with DNA."
    Parkinson M.J., Lilley D.M.
    J. Mol. Biol. 270:169-178(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, FUNCTION.
  5. "The complex between a four-way DNA junction and T7 endonuclease I."
    Declais A.C., Fogg J.M., Freeman A.D., Coste F., Hadden J.M., Phillips S.E., Lilley D.M.
    EMBO J. 22:1398-1409(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The importance of the N-terminus of T7 endonuclease I in the interaction with DNA junctions."
    Freeman A.D., Declais A.C., Lilley D.M.
    J. Mol. Biol. 425:395-410(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I."
    Hadden J.M., Convery M.A., Declais A.C., Lilley D.M., Phillips S.E.
    Nat. Struct. Biol. 8:62-67(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 12-149.
  8. "Metal ions bound at the active site of the junction-resolving enzyme T7 endonuclease I."
    Hadden J.M., Declais A.C., Phillips S.E., Lilley D.M.
    EMBO J. 21:3505-3515(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 12-149.
  9. "The structural basis of Holliday junction resolution by T7 endonuclease I."
    Hadden J.M., Declais A.C., Carr S.B., Lilley D.M., Phillips S.E.
    Nature 449:621-624(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
  10. "The structure of a fibril-forming sequence, NNQQNY, in the context of a globular fold."
    Guo Z., Eisenberg D.
    Protein Sci. 17:1617-1623(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 18-145.

Entry informationi

Entry nameiENDO_BPT7
AccessioniPrimary (citable) accession number: P00641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: December 9, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.