Reviewed, UniProtKB/Swiss-Prot P00641 (ENRN_BPT7)

Names and origin

Protein names

Recommended name:
    Endodeoxyribonuclease 1
    EC=3.1.21.2
Alternative name(s):
    Endodeoxyribonuclease I
      Short name=Endonuclease

Gene names

Name:

3

Organism

Enterobacteria phage T7 (Bacteriophage T7)

Taxonomic identifier

10760 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Endodeoxyribonuclease I, which is expressed in the late stage, is necessary for T3 genetic recombination and the breakdown of host DNA. In the early stage of infection, T3 DNA replicates as a linear monomer. In the late stage, the T3 DNA replicates via linear concatemers several genomes in length. The gene 3 product has also been implicated in the maturation of these concatemers.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro viral reproduction Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro deoxyribonuclease IV (phage-T4-induced) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 149

149

Endodeoxyribonuclease 1

PRO_0000106486

Secondary structure

1

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149

Helix Strand Turn

Details...

Helix

18 – 29

12

Beta strand

35 – 37

3

Beta strand

39 – 45

7

Beta strand

48 – 52

5

Beta strand

55 – 57

3

Beta strand

61 – 69

9

Helix

72 – 84

13

Beta strand

89 – 95

7

Beta strand

99 – 102

4

Helix

109 – 115

7

Beta strand

120 – 124

5

Helix

127 – 131

5

Helix

139 – 141

3

Beta strand

142 – 144

3

Sequences

	Sequence		Length	Mass (Da)
	P00641-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: D092AA28E3743BC1	FASTA	149	17,172
	10 20 30 40 50 60 MAGYGAKGIR KVGAFRSGLE DKVSKQLESK GIKFEYEEWK VPYVIPASNH TYTPDFLLPN 70 80 90 100 110 120 GIFVETKGLW ESDDRKKHLL IREQHPELDI RIVFSSSRTK LYKGSPTSYG EFCEKHGIKF 130 140 ADKLIPAEWI KEPKKEVPFD RLKRKGGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements." Dunn J.J., Studier F.W. J. Mol. Biol. 166:477-535(1983) [PubMed: 6864790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4." Dunn J.J., Studier F.W. J. Mol. Biol. 148:303-330(1981) [PubMed: 7310871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

V01127 Genomic DNA. Translation: CAA24345.1.
V01146 Genomic DNA. Translation: CAA24402.1.

PIR

NEBP37. B94615.

RefSeq

NP_041972.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FZR	X-ray	2.10	A/B/C/D	12-149	[»]
1M0D	X-ray	1.90	A/B/C/D	12-149	[»]
1M0I	X-ray	2.55	A/B/C/D	12-149	[»]
2PFJ	X-ray	3.10	A/B	1-149	[»]
3CAE	X-ray	3.00	A/B/C/D/E/F/G/H/I/J	18-145	[»]

ModBase

Search...

Genome annotation databases

GeneID

1261079.

GenomeReviews

Gene locus 3 in contig V01146_GR.

Enzyme and pathway databases

BRENDA

3.1.21.2. 1115.

Family and domain databases

InterPro

IPR008029. Endonuclease-I_phage.
IPR011578. Restrict_endonuc_C/endonuc_I.
[Graphical view]

Gene3D

G3DSA:3.40.91.30. Restrict_endonuc_C/endonuc_I. 1 hit.

Pfam

PF05367. Phage_endo_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ENRN_BPT7

Accession

Primary (citable) accession number: P00641

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)