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Protein

Endonuclease I

Gene

3

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.4 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Bacterial host gene expression shutoff by virus, Degradation of host chromosome by virus, Host gene expression shutoff by virus, Host-virus interaction

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease I (EC:3.1.21.2)
Alternative name(s):
Gene product 3
Short name:
Gp3
Junction-resolving enzyme gp3
Gene namesi
Ordered Locus Names:3
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001064861 – 149Endonuclease IAdd BLAST149

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-41668N.
IntActiP00641. 1 interactor.
MINTiMINT-1513471.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 29Combined sources12
Beta strandi35 – 37Combined sources3
Beta strandi39 – 45Combined sources7
Beta strandi48 – 52Combined sources5
Beta strandi55 – 57Combined sources3
Beta strandi63 – 69Combined sources7
Helixi72 – 84Combined sources13
Beta strandi90 – 95Combined sources6
Beta strandi99 – 102Combined sources4
Helixi109 – 116Combined sources8
Beta strandi120 – 124Combined sources5
Helixi127 – 131Combined sources5
Helixi139 – 141Combined sources3
Beta strandi142 – 144Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZRX-ray2.10A/B/C/D12-149[»]
1M0DX-ray1.90A/B/C/D12-149[»]
1M0IX-ray2.55A/B/C/D12-149[»]
2PFJX-ray3.10A/B1-149[»]
3CAEX-ray3.00A/B/C/D/E/F/G/H/I/J18-145[»]
ProteinModelPortaliP00641.
SMRiP00641.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00641.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.30. 1 hit.
InterProiIPR008029. Phage_T7_Gp3_endoDNaseI.
IPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
[Graphical view]
PfamiPF05367. Phage_endo_I. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P00641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGYGAKGIR KVGAFRSGLE DKVSKQLESK GIKFEYEEWK VPYVIPASNH
60 70 80 90 100
TYTPDFLLPN GIFVETKGLW ESDDRKKHLL IREQHPELDI RIVFSSSRTK
110 120 130 140
LYKGSPTSYG EFCEKHGIKF ADKLIPAEWI KEPKKEVPFD RLKRKGGKK
Length:149
Mass (Da):17,172
Last modified:July 21, 1986 - v1
Checksum:iD092AA28E3743BC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01127 Genomic DNA. Translation: CAA24345.1.
V01146 Genomic DNA. Translation: CAA24402.1.
PIRiB94615. NEBP37.
RefSeqiNP_041972.1. NC_001604.1.

Genome annotation databases

GeneIDi1261079.
KEGGivg:1261079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01127 Genomic DNA. Translation: CAA24345.1.
V01146 Genomic DNA. Translation: CAA24402.1.
PIRiB94615. NEBP37.
RefSeqiNP_041972.1. NC_001604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZRX-ray2.10A/B/C/D12-149[»]
1M0DX-ray1.90A/B/C/D12-149[»]
1M0IX-ray2.55A/B/C/D12-149[»]
2PFJX-ray3.10A/B1-149[»]
3CAEX-ray3.00A/B/C/D/E/F/G/H/I/J18-145[»]
ProteinModelPortaliP00641.
SMRiP00641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41668N.
IntActiP00641. 1 interactor.
MINTiMINT-1513471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261079.
KEGGivg:1261079.

Miscellaneous databases

EvolutionaryTraceiP00641.

Family and domain databases

Gene3Di3.40.91.30. 1 hit.
InterProiIPR008029. Phage_T7_Gp3_endoDNaseI.
IPR011335. Restrct_endonuc-II-like.
IPR011578. Restrct_endonuc_C/endonuc_I.
[Graphical view]
PfamiPF05367. Phage_endo_I. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENDO_BPT7
AccessioniPrimary (citable) accession number: P00641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.