ID   T2PS_PROST              Reviewed;         326 AA.
AC   P00640;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-MAY-2023, entry version 88.
DE   RecName: Full=Type II restriction enzyme PstI {ECO:0000303|PubMed:12654995};
DE            Short=R.PstI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease PstI;
DE   AltName: Full=Type-2 restriction enzyme PstI;
GN   Name=pstIR;
OS   Providencia stuartii.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Providencia.
OX   NCBI_TaxID=588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, FUNCTION, AND
RP   SUBUNIT.
RC   STRAIN=164;
RX   PubMed=6330092; DOI=10.1016/s0021-9258(17)42896-1;
RA   Walder R.Y., Walder J.A., Donelson J.E.;
RT   "The organization and complete nucleotide sequence of the PstI restriction-
RT   modification system.";
RL   J. Biol. Chem. 259:8015-8026(1984).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-CTGCAG-3' and cleaves after A-5.
CC       {ECO:0000269|PubMed:6330092, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:6330092}.
CC   -!- SIMILARITY: Belongs to the BsuBI/PstI type II restriction endonuclease
CC       family. {ECO:0000305}.
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DR   EMBL; K02081; AAA25673.1; -; Genomic_DNA.
DR   PIR; A00783; NDOFS.
DR   AlphaFoldDB; P00640; -.
DR   SMR; P00640; -.
DR   BindingDB; P00640; -.
DR   ChEMBL; CHEMBL5713; -.
DR   BRENDA; 3.1.21.4; 5058.
DR   PRO; PR:P00640; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1350.80; -; 1.
DR   Gene3D; 1.10.10.1820; BsuBI/PstI restriction endonuclease-like; 1.
DR   InterPro; IPR041963; BsuBI/PstI_C_sf.
DR   InterPro; IPR041454; BsuBI/PstI_N.
DR   InterPro; IPR041962; BsuBI/PstI_N_sf.
DR   InterPro; IPR009528; Restrct_endonuc_II_BsuBI_C.
DR   Pfam; PF06616; BsuBI_PstI_RE; 1.
DR   Pfam; PF17728; BsuBI_PstI_RE_N; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Restriction system.
FT   CHAIN           1..326
FT                   /note="Type II restriction enzyme PstI"
FT                   /id="PRO_0000077354"
SQ   SEQUENCE   326 AA;  37414 MW;  475841183B414504 CRC64;
     MKELKLKEAK EILKALGLPP QQYNDRSGWV LLALANIKPE DSWKEAKAPL LPTVSIMEFI
     RTEYGKDYKP NSRETIRRQT LHQFEQARIV DRNRDLPSRA TNSKDNNYSL NQVIIDILHN
     YPNGNWKELI QQFLTHVPSL QELYERALAR DRIPIKLLDG TQISLSPGEH NQLHADIVHE
     FCPRFVGDMG KILYIGDTAS SRNEGGKLMV LDSEYLKKLG VPPMSHDKLP DVVVYDEKRK
     WLFLIEAVTS HGPISPKRWL ELEAALSSCT VGKVYVTAFP TRTEFRKNAA NIAWETEVWI
     ADNPDHMVHF NGDRFLGPHD KKPELS
//