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P00640 (T2PS_PROST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme PstI

Short name=R.PstI
EC=3.1.21.4
Alternative name(s):
Endonuclease PstI
Type II restriction enzyme PstI
Gene names
Name:pstIR
OrganismProvidencia stuartii
Taxonomic identifier588 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the BsuBI/PstI restriction endonuclease family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Type-2 restriction enzyme PstI
PRO_0000077354

Sequences

Sequence LengthMass (Da)Tools
P00640 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 475841183B414504

FASTA32637,414
        10         20         30         40         50         60 
MKELKLKEAK EILKALGLPP QQYNDRSGWV LLALANIKPE DSWKEAKAPL LPTVSIMEFI 

        70         80         90        100        110        120 
RTEYGKDYKP NSRETIRRQT LHQFEQARIV DRNRDLPSRA TNSKDNNYSL NQVIIDILHN 

       130        140        150        160        170        180 
YPNGNWKELI QQFLTHVPSL QELYERALAR DRIPIKLLDG TQISLSPGEH NQLHADIVHE 

       190        200        210        220        230        240 
FCPRFVGDMG KILYIGDTAS SRNEGGKLMV LDSEYLKKLG VPPMSHDKLP DVVVYDEKRK 

       250        260        270        280        290        300 
WLFLIEAVTS HGPISPKRWL ELEAALSSCT VGKVYVTAFP TRTEFRKNAA NIAWETEVWI 

       310        320 
ADNPDHMVHF NGDRFLGPHD KKPELS 

« Hide

References

[1]"The organization and complete nucleotide sequence of the PstI restriction-modification system."
Walder R.Y., Walder J.A., Donelson J.E.
J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 164.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02081 Genomic DNA. Translation: AAA25673.1.
PIRNDOFS. A00783.

3D structure databases

ProteinModelPortalP00640.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP00640.
ChEMBLCHEMBL5713.

Protein family/group databases

REBASE1536. PstI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view]
PfamPF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2PS_PROST
AccessionPrimary (citable) accession number: P00640
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries