P00640 (T2PS_PROST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme PstI
Short name=R.PstI
EC=3.1.21.4

Alternative name(s):
Endonuclease PstI
Type II restriction enzyme PstI

Gene names

Name:

pstIR

Organism

Providencia stuartii

Taxonomic identifier

588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Providencia

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Homodimer.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Type-2 restriction enzyme PstI		PRO_0000077354

Sequences

	Sequence		Length	Mass (Da)
	P00640 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 475841183B414504	FASTA	326	37,414
	10 20 30 40 50 60 MKELKLKEAK EILKALGLPP QQYNDRSGWV LLALANIKPE DSWKEAKAPL LPTVSIMEFI 70 80 90 100 110 120 RTEYGKDYKP NSRETIRRQT LHQFEQARIV DRNRDLPSRA TNSKDNNYSL NQVIIDILHN 130 140 150 160 170 180 YPNGNWKELI QQFLTHVPSL QELYERALAR DRIPIKLLDG TQISLSPGEH NQLHADIVHE 190 200 210 220 230 240 FCPRFVGDMG KILYIGDTAS SRNEGGKLMV LDSEYLKKLG VPPMSHDKLP DVVVYDEKRK 250 260 270 280 290 300 WLFLIEAVTS HGPISPKRWL ELEAALSSCT VGKVYVTAFP TRTEFRKNAA NIAWETEVWI 310 320 ADNPDHMVHF NGDRFLGPHD KKPELS

References

[1]	"The organization and complete nucleotide sequence of the PstI restriction-modification system." Walder R.Y., Walder J.A., Donelson J.E. J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 164.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02081 Genomic DNA. Translation: AAA25673.1.
PIR	NDOFS. A00783.
3D structure databases
ProteinModelPortal	P00640.
ModBase	Search...
Protein family/group databases
REBASE	1536. PstI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009528. Restrct_endonuc_II_BsuBI_C. [Graphical view]
Pfam	PF06616. BsuBI_PstI_RE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P00640.
ChEMBL	CHEMBL5713.

Entry information

Entry name

T2PS_PROST

Accession

Primary (citable) accession number: P00640

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program