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P00640

- T2PS_PROST

UniProt

P00640 - T2PS_PROST

Protein

Type-2 restriction enzyme PstI

Gene

pstIR

Organism
Providencia stuartii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    • Comment

    Functioni

    Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1536. PstI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme PstI (EC:3.1.21.4)
    Short name:
    R.PstI
    Alternative name(s):
    Endonuclease PstI
    Type II restriction enzyme PstI
    Gene namesi
    Name:pstIR
    OrganismiProvidencia stuartii
    Taxonomic identifieri588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Type-2 restriction enzyme PstIPRO_0000077354Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP00640.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR009528. Restrct_endonuc_II_BsuBI_C.
    [Graphical view]
    PfamiPF06616. BsuBI_PstI_RE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00640-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKELKLKEAK EILKALGLPP QQYNDRSGWV LLALANIKPE DSWKEAKAPL    50
    LPTVSIMEFI RTEYGKDYKP NSRETIRRQT LHQFEQARIV DRNRDLPSRA 100
    TNSKDNNYSL NQVIIDILHN YPNGNWKELI QQFLTHVPSL QELYERALAR 150
    DRIPIKLLDG TQISLSPGEH NQLHADIVHE FCPRFVGDMG KILYIGDTAS 200
    SRNEGGKLMV LDSEYLKKLG VPPMSHDKLP DVVVYDEKRK WLFLIEAVTS 250
    HGPISPKRWL ELEAALSSCT VGKVYVTAFP TRTEFRKNAA NIAWETEVWI 300
    ADNPDHMVHF NGDRFLGPHD KKPELS 326
    Length:326
    Mass (Da):37,414
    Last modified:July 21, 1986 - v1
    Checksum:i475841183B414504
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02081 Genomic DNA. Translation: AAA25673.1.
    PIRiA00783. NDOFS.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02081 Genomic DNA. Translation: AAA25673.1 .
    PIRi A00783. NDOFS.

    3D structure databases

    ProteinModelPortali P00640.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P00640.
    ChEMBLi CHEMBL5713.

    Protein family/group databases

    REBASEi 1536. PstI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR009528. Restrct_endonuc_II_BsuBI_C.
    [Graphical view ]
    Pfami PF06616. BsuBI_PstI_RE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization and complete nucleotide sequence of the PstI restriction-modification system."
      Walder R.Y., Walder J.A., Donelson J.E.
      J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 164.

    Entry informationi

    Entry nameiT2PS_PROST
    AccessioniPrimary (citable) accession number: P00640
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3