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Protein

Deoxyribonuclease-1

Gene

DNASE1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:4976790, PubMed:5166750, PubMed:3352748, PubMed:2395459). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed:2395459). Binds specifically to G-actin and blocks actin polymerization (PubMed:2395459). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).By similarity4 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.3 Publications

Cofactori

Ca2+3 Publications, Mg2+3 PublicationsNote: Divalent metal cations. Prefers Ca2+ or Mg2+.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei35Involved in actin-binding1 Publication1
Sitei87Nitration by tetranitromethane destroys a Ca(2+) binding site and inactivates enzyme1
Sitei89Involved in actin-binding1 Publication1
Active sitei1001 Publication1
Active sitei1562 Publications1

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • DNA catabolic process, endonucleolytic Source: UniProtKB
  • neutrophil activation involved in immune response Source: UniProtKB
  • regulation of acute inflammatory response Source: UniProtKB
  • regulation of neutrophil mediated cytotoxicity Source: UniProtKB

Keywordsi

Molecular functionActin-binding, Endonuclease, Hydrolase, Nuclease
Biological processApoptosis
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.21.1 908

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.13 Publications)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:DNASE1
Synonyms:DNL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Organism-specific databases

VGNCiVGNC:28132 DNASE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5712

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000000727523 – 282Deoxyribonuclease-1Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi123 ↔ 1261 Publication
Disulfide bondi195 ↔ 231Essential for enzymatic activity1 Publication

Post-translational modificationi

The only differences between the A and B forms and the C and D forms are in the compositions of the carbohydrate bound to Asn-40.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP00639
PRIDEiP00639

Expressioni

Gene expression databases

BgeeiENSBTAG00000020107
ExpressionAtlasiP00639 baseline

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681353EBI-8545986,EBI-367540From Oryctolagus cuniculus.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-541N
IntActiP00639, 3 interactors
MINTiP00639
STRINGi9913.ENSBTAP00000054758

Chemistry databases

BindingDBiP00639

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 34Combined sources11
Helixi35 – 39Combined sources5
Helixi41 – 51Combined sources11
Beta strandi55 – 62Combined sources8
Beta strandi65 – 67Combined sources3
Helixi68 – 77Combined sources10
Beta strandi79 – 81Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi95 – 97Combined sources3
Beta strandi100 – 106Combined sources7
Turni108 – 110Combined sources3
Beta strandi112 – 118Combined sources7
Beta strandi121 – 123Combined sources3
Turni124 – 126Combined sources3
Helixi127 – 130Combined sources4
Beta strandi136 – 141Combined sources6
Beta strandi145 – 154Combined sources10
Helixi159 – 161Combined sources3
Helixi162 – 180Combined sources19
Beta strandi185 – 190Combined sources6
Turni195 – 197Combined sources3
Helixi200 – 205Combined sources6
Helixi207 – 210Combined sources4
Beta strandi214 – 218Combined sources5
Beta strandi225 – 228Combined sources4
Beta strandi234 – 240Combined sources7
Helixi241 – 246Combined sources6
Helixi257 – 260Combined sources4
Helixi265 – 271Combined sources7
Beta strandi277 – 281Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ATNX-ray2.80D23-282[»]
1DNKX-ray2.30A23-282[»]
2A3ZX-ray2.08B23-282[»]
2A40X-ray1.80B/E23-282[»]
2A41X-ray2.60B23-282[»]
2A42X-ray1.85B23-282[»]
2D1KX-ray2.50B23-282[»]
2DNJX-ray2.00A23-282[»]
3CJCX-ray3.90D23-282[»]
3DNIX-ray2.00A23-282[»]
3W3DX-ray1.80B23-282[»]
ProteinModelPortaliP00639
SMRiP00639
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00639

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFZB Eukaryota
ENOG410Z4MV LUCA
GeneTreeiENSGT00390000013146
HOGENOMiHOG000059570
HOVERGENiHBG051368
InParanoidiP00639
KOiK11994

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR018057 Deoxyribonuclease-1_AS
IPR016202 DNase_I
IPR033125 DNASE_I_2
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PIRSFiPIRSF000988 DNase_I_euk, 1 hit
PRINTSiPR00130 DNASEI
SMARTiView protein in SMART
SM00476 DNaseIc, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS00919 DNASE_I_1, 1 hit
PS00918 DNASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGTRLMGLL LALAGLLQLG LSLKIAAFNI RTFGETKMSN ATLASYIVRI
60 70 80 90 100
VRRYDIVLIQ EVRDSHLVAV GKLLDYLNQD DPNTYHYVVS EPLGRNSYKE
110 120 130 140 150
RYLFLFRPNK VSVLDTYQYD DGCESCGNDS FSREPAVVKF SSHSTKVKEF
160 170 180 190 200
AIVALHSAPS DAVAEINSLY DVYLDVQQKW HLNDVMLMGD FNADCSYVTS
210 220 230 240 250
SQWSSIRLRT SSTFQWLIPD SADTTATSTN CAYDRIVVAG SLLQSSVVPG
260 270 280
SAAPFDFQAA YGLSNEMALA ISDHYPVEVT LT
Length:282
Mass (Da):31,346
Last modified:December 15, 1998 - v3
Checksum:i43904EF0D5F2E0E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217L → V in AAM93248 (PubMed:14680812).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti143H → P in allele C/D. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001538 mRNA Translation: CAA04819.1
AF528509 Genomic DNA Translation: AAM93248.1
BC142349 mRNA Translation: AAI42350.1
PIRiJC6532 NDBOA
RefSeqiNP_776959.1, NM_174534.2
XP_010817356.1, XM_010819054.2
XP_015315660.1, XM_015460174.1
XP_015315661.1, XM_015460175.1
XP_015315662.1, XM_015460176.1
XP_015315663.1, XM_015460177.1
UniGeneiBt.12952

Genome annotation databases

EnsembliENSBTAT00000026784; ENSBTAP00000026784; ENSBTAG00000020107
GeneIDi282217
KEGGibta:282217

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDNAS1_BOVIN
AccessioniPrimary (citable) accession number: P00639
Secondary accession number(s): A5PK44, Q8MJ27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health