Repressible acid phosphatase precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P00635 (PPA5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Repressible acid phosphatase
EC=3.1.3.2

Alternative name(s):
P60

Gene names

Name:	PHO5
Ordered Locus Names:	YBR093C
ORF Names:	YBR0814

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Partially mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and NPP2. Ref.8
Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate. Ref.1 Ref.6
Subcellular location	Secreted.
Induction	Expression induced during phosphate starvation. Repressed by inorganic phosphate. Ref.8
Post-translational modification	Glycosylated during secretion across the membrane.
Miscellaneous	Present with 1110 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to phosphate starvation Inferred from mutant phenotype Ref.8. Source: SGD dephosphorylation Inferred from electronic annotation. Source: GOC regulation of cell size Inferred from mutant phenotype PubMed 12089449. Source: SGD
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type cell wall Inferred from direct assay PubMed 3537693. Source: SGD
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC nucleoside-triphosphatase activity Inferred from mutant phenotype Ref.8. Source: SGD nucleoside-triphosphate diphosphatase activity Inferred from mutant phenotype Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

17

Chain

450

Repressible acid phosphatase

PRO_0000023954

Sites

Active site

1

Nucleophile By similarity

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1

D → Y in CAA24630. Ref.1

Sequence conflict

1

A → G in CAA24630. Ref.1

Sequence conflict

1

H → Q in CAA24630. Ref.1

Sequence conflict

1

S → V in CAA24630. Ref.1

Sequence conflict

2

AS → DT in CAA24630. Ref.1

Sequence conflict

1

R → K in CAA24630. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P00635 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: DC3C9504BC2D3D0C
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467

52,859

        10         20         30         40         50         60 
MFKSVVYSIL AASLANAGTI PLGKLADVDK IGTQKDIFPF LGGAGPYYSF PGDYGISRDL 

        70         80         90        100        110        120 
PEGCEMKQLQ MVGRHGERYP TVSLAKTIKS TWYKLSNYTR QFNGSLSFLN DDYEFFIRDD 

       130        140        150        160        170        180 
DDLEMETTFA NSDDVLNPYT GEMNAKRHAR DFLAQYGYMV ENQTSFAVFT SNSKRCHDTA 

       190        200        210        220        230        240 
QYFIDGLGDQ FNITLQTVSE AESAGANTLS ACNSCPAWDY DANDDIVNEY DTTYLDDIAK 

       250        260        270        280        290        300 
RLNKENKGLN LTSTDASTLF SWCAFEVNAK GYSDVCDIFT KDELVHYSYY QDLHTYYHEG 

       310        320        330        340        350        360 
PGYDIIKSVG SNLFNASVKL LKQSEIQDQK VWLSFTHDTD ILNFLTTAGI IDDKNNLTAE 

       370        380        390        400        410        420 
YVPFMGNTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND AVVPIETCST GPGFSCEIND 

       430        440        450        460 
FYDYAEKRVA GTDFLKVCNV SSVSNSTELT FYWDWNTTHY NASLLRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide." Arima K., Oshima T., Kubota I., Nakamura N., Mizunaga T., Toh-e A. Nucleic Acids Res. 11:1657-1672(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-26, CATALYTIC ACTIVITY.
[2]	"Structural analysis of the two tandemly repeated acid phosphatase genes in yeast." Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A. Nucleic Acids Res. 12:7721-7739(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-45.
[3]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae." Tait-Kamradt A.G., Turner K.J., Kramer R.A., Elliott Q.D., Bostian S.J., Thill G.P., Rogers D.T., Bostian K.A. Mol. Cell. Biol. 6:1855-1865(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44, CATALYTIC ACTIVITY, REPRESSION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae." Kennedy E.J., Pillus L., Ghosh G. Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01320 Genomic DNA. Translation: CAA24630.1. X01079 Genomic DNA. Translation: CAA25555.1. X78993 Genomic DNA. Translation: CAA55598.1. Z35962 Genomic DNA. Translation: CAA85046.1. X01080 Genomic DNA. Translation: CAA25556.1. BK006936 Genomic DNA. Translation: DAA07214.1.
PIR	PABYC. S05795.
RefSeq	NP_009651.3. NM_001178441.3.
3D structure databases
ProteinModelPortal	P00635.
SMR	P00635. Positions 37-458.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4916N.
IntAct	P00635. 6 interactions.
MINT	MINT-478455.
STRING	4932.YBR093C.
Proteomic databases
PaxDb	P00635.
PeptideAtlas	P00635.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR093C; YBR093C; YBR093C.
GeneID	852390.
KEGG	sce:YBR093C.
Organism-specific databases
CYGD	YBR093c.
SGD	S000000297. PHO5.
Phylogenomic databases
eggNOG	NOG260296.
GeneTree	ENSGT00510000050349.
HOGENOM	HOG000189191.
KO	K01078.
OMA	YVRFIAN.
OrthoDB	EOG4SXRMX.
Enzyme and pathway databases
BioCyc	YEAST:YBR093C-MONOMER.
Gene expression databases
ArrayExpress	P00635.
Genevestigator	P00635.
GermOnline	YBR093C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971207.

Entry information

Entry name

PPA5_YEAST

Accession

Primary (citable) accession number: P00635
Secondary accession number(s): D6VQ94

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents