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P00635

- PPA5_YEAST

UniProt

P00635 - PPA5_YEAST

Protein

Repressible acid phosphatase

Gene

PHO5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Partially mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and NPP2.1 Publication

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751NucleophileBy similarity
    Active sitei338 – 3381Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: SGD
    2. nucleoside-triphosphatase activity Source: SGD
    3. nucleoside-triphosphate diphosphatase activity Source: SGD

    GO - Biological processi

    1. cellular response to phosphate starvation Source: SGD
    2. dephosphorylation Source: GOC
    3. phosphate-containing compound metabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:YBR093C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Repressible acid phosphatase (EC:3.1.3.2)
    Alternative name(s):
    P60
    Gene namesi
    Name:PHO5
    Ordered Locus Names:YBR093C
    ORF Names:YBR0814
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR093c.
    SGDiS000000297. PHO5.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17172 PublicationsAdd
    BLAST
    Chaini18 – 467450Repressible acid phosphatasePRO_0000023954Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated during secretion across the membrane.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP00635.
    PaxDbiP00635.
    PeptideAtlasiP00635.

    Expressioni

    Inductioni

    Expression induced during phosphate starvation. Repressed by inorganic phosphate.2 Publications

    Gene expression databases

    GenevestigatoriP00635.

    Interactioni

    Protein-protein interaction databases

    BioGridi32799. 37 interactions.
    DIPiDIP-4916N.
    IntActiP00635. 6 interactions.
    MINTiMINT-478455.
    STRINGi4932.YBR093C.

    Structurei

    3D structure databases

    ProteinModelPortaliP00635.
    SMRiP00635. Positions 37-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG260296.
    GeneTreeiENSGT00510000050349.
    HOGENOMiHOG000189191.
    KOiK01078.
    OMAiRYVINDS.
    OrthoDBiEOG7TQV9N.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00635-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKSVVYSIL AASLANAGTI PLGKLADVDK IGTQKDIFPF LGGAGPYYSF    50
    PGDYGISRDL PEGCEMKQLQ MVGRHGERYP TVSLAKTIKS TWYKLSNYTR 100
    QFNGSLSFLN DDYEFFIRDD DDLEMETTFA NSDDVLNPYT GEMNAKRHAR 150
    DFLAQYGYMV ENQTSFAVFT SNSKRCHDTA QYFIDGLGDQ FNITLQTVSE 200
    AESAGANTLS ACNSCPAWDY DANDDIVNEY DTTYLDDIAK RLNKENKGLN 250
    LTSTDASTLF SWCAFEVNAK GYSDVCDIFT KDELVHYSYY QDLHTYYHEG 300
    PGYDIIKSVG SNLFNASVKL LKQSEIQDQK VWLSFTHDTD ILNFLTTAGI 350
    IDDKNNLTAE YVPFMGNTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND 400
    AVVPIETCST GPGFSCEIND FYDYAEKRVA GTDFLKVCNV SSVSNSTELT 450
    FYWDWNTTHY NASLLRQ 467
    Length:467
    Mass (Da):52,859
    Last modified:October 1, 1994 - v2
    Checksum:iDC3C9504BC2D3D0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361D → Y in CAA24630. (PubMed:6300772)Curated
    Sequence conflicti130 – 1301A → G in CAA24630. (PubMed:6300772)Curated
    Sequence conflicti294 – 2941H → Q in CAA24630. (PubMed:6300772)Curated
    Sequence conflicti446 – 4461S → V in CAA24630. (PubMed:6300772)Curated
    Sequence conflicti462 – 4632AS → DT in CAA24630. (PubMed:6300772)Curated
    Sequence conflicti466 – 4661R → K in CAA24630. (PubMed:6300772)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01320 Genomic DNA. Translation: CAA24630.1.
    X01079 Genomic DNA. Translation: CAA25555.1.
    X78993 Genomic DNA. Translation: CAA55598.1.
    Z35962 Genomic DNA. Translation: CAA85046.1.
    X01080 Genomic DNA. Translation: CAA25556.1.
    BK006936 Genomic DNA. Translation: DAA07214.1.
    PIRiS05795. PABYC.
    RefSeqiNP_009651.3. NM_001178441.3.

    Genome annotation databases

    EnsemblFungiiYBR093C; YBR093C; YBR093C.
    GeneIDi852390.
    KEGGisce:YBR093C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01320 Genomic DNA. Translation: CAA24630.1 .
    X01079 Genomic DNA. Translation: CAA25555.1 .
    X78993 Genomic DNA. Translation: CAA55598.1 .
    Z35962 Genomic DNA. Translation: CAA85046.1 .
    X01080 Genomic DNA. Translation: CAA25556.1 .
    BK006936 Genomic DNA. Translation: DAA07214.1 .
    PIRi S05795. PABYC.
    RefSeqi NP_009651.3. NM_001178441.3.

    3D structure databases

    ProteinModelPortali P00635.
    SMRi P00635. Positions 37-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32799. 37 interactions.
    DIPi DIP-4916N.
    IntActi P00635. 6 interactions.
    MINTi MINT-478455.
    STRINGi 4932.YBR093C.

    Proteomic databases

    MaxQBi P00635.
    PaxDbi P00635.
    PeptideAtlasi P00635.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR093C ; YBR093C ; YBR093C .
    GeneIDi 852390.
    KEGGi sce:YBR093C.

    Organism-specific databases

    CYGDi YBR093c.
    SGDi S000000297. PHO5.

    Phylogenomic databases

    eggNOGi NOG260296.
    GeneTreei ENSGT00510000050349.
    HOGENOMi HOG000189191.
    KOi K01078.
    OMAi RYVINDS.
    OrthoDBi EOG7TQV9N.

    Enzyme and pathway databases

    BioCyci YEAST:YBR093C-MONOMER.

    Miscellaneous databases

    NextBioi 971207.

    Gene expression databases

    Genevestigatori P00635.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide."
      Arima K., Oshima T., Kubota I., Nakamura N., Mizunaga T., Toh-e A.
      Nucleic Acids Res. 11:1657-1672(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-26, CATALYTIC ACTIVITY.
    2. "Structural analysis of the two tandemly repeated acid phosphatase genes in yeast."
      Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A.
      Nucleic Acids Res. 12:7721-7739(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-45.
    3. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae."
      Tait-Kamradt A.G., Turner K.J., Kramer R.A., Elliott Q.D., Bostian S.J., Thill G.P., Rogers D.T., Bostian K.A.
      Mol. Cell. Biol. 6:1855-1865(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44, CATALYTIC ACTIVITY, REPRESSION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
      Kennedy E.J., Pillus L., Ghosh G.
      Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiPPA5_YEAST
    AccessioniPrimary (citable) accession number: P00635
    Secondary accession number(s): D6VQ94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1110 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3