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P00635

- PPA5_YEAST

UniProt

P00635 - PPA5_YEAST

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Protein
Repressible acid phosphatase
Gene
PHO5, YBR093C, YBR0814
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Partially mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and NPP2.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Nucleophile By similarity
Active sitei338 – 3381Proton donor By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: SGD
  2. nucleoside-triphosphatase activity Source: SGD
  3. nucleoside-triphosphate diphosphatase activity Source: SGD

GO - Biological processi

  1. cellular response to phosphate starvation Source: SGD
  2. dephosphorylation Source: GOC
  3. phosphate-containing compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YBR093C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Repressible acid phosphatase (EC:3.1.3.2)
Alternative name(s):
P60
Gene namesi
Name:PHO5
Ordered Locus Names:YBR093C
ORF Names:YBR0814
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR093c.
SGDiS000000297. PHO5.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17172 Publications
Add
BLAST
Chaini18 – 467450Repressible acid phosphatase
PRO_0000023954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi103 – 1031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi162 – 1621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi192 – 1921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi250 – 2501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi315 – 3151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi356 – 3561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi390 – 3901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi439 – 4391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi445 – 4451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi456 – 4561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi461 – 4611N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated during secretion across the membrane.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP00635.
PaxDbiP00635.
PeptideAtlasiP00635.

Expressioni

Inductioni

Expression induced during phosphate starvation. Repressed by inorganic phosphate.1 Publication

Gene expression databases

GenevestigatoriP00635.

Interactioni

Protein-protein interaction databases

BioGridi32799. 37 interactions.
DIPiDIP-4916N.
IntActiP00635. 6 interactions.
MINTiMINT-478455.
STRINGi4932.YBR093C.

Structurei

3D structure databases

ProteinModelPortaliP00635.
SMRiP00635. Positions 37-458.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG260296.
GeneTreeiENSGT00510000050349.
HOGENOMiHOG000189191.
KOiK01078.
OMAiRYVINDS.
OrthoDBiEOG7TQV9N.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00635-1 [UniParc]FASTAAdd to Basket

« Hide

MFKSVVYSIL AASLANAGTI PLGKLADVDK IGTQKDIFPF LGGAGPYYSF    50
PGDYGISRDL PEGCEMKQLQ MVGRHGERYP TVSLAKTIKS TWYKLSNYTR 100
QFNGSLSFLN DDYEFFIRDD DDLEMETTFA NSDDVLNPYT GEMNAKRHAR 150
DFLAQYGYMV ENQTSFAVFT SNSKRCHDTA QYFIDGLGDQ FNITLQTVSE 200
AESAGANTLS ACNSCPAWDY DANDDIVNEY DTTYLDDIAK RLNKENKGLN 250
LTSTDASTLF SWCAFEVNAK GYSDVCDIFT KDELVHYSYY QDLHTYYHEG 300
PGYDIIKSVG SNLFNASVKL LKQSEIQDQK VWLSFTHDTD ILNFLTTAGI 350
IDDKNNLTAE YVPFMGNTFH RSWYVPQGAR VYTEKFQCSN DTYVRYVIND 400
AVVPIETCST GPGFSCEIND FYDYAEKRVA GTDFLKVCNV SSVSNSTELT 450
FYWDWNTTHY NASLLRQ 467
Length:467
Mass (Da):52,859
Last modified:October 1, 1994 - v2
Checksum:iDC3C9504BC2D3D0C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361D → Y in CAA24630. 1 Publication
Sequence conflicti130 – 1301A → G in CAA24630. 1 Publication
Sequence conflicti294 – 2941H → Q in CAA24630. 1 Publication
Sequence conflicti446 – 4461S → V in CAA24630. 1 Publication
Sequence conflicti462 – 4632AS → DT in CAA24630. 1 Publication
Sequence conflicti466 – 4661R → K in CAA24630. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01320 Genomic DNA. Translation: CAA24630.1.
X01079 Genomic DNA. Translation: CAA25555.1.
X78993 Genomic DNA. Translation: CAA55598.1.
Z35962 Genomic DNA. Translation: CAA85046.1.
X01080 Genomic DNA. Translation: CAA25556.1.
BK006936 Genomic DNA. Translation: DAA07214.1.
PIRiS05795. PABYC.
RefSeqiNP_009651.3. NM_001178441.3.

Genome annotation databases

EnsemblFungiiYBR093C; YBR093C; YBR093C.
GeneIDi852390.
KEGGisce:YBR093C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01320 Genomic DNA. Translation: CAA24630.1 .
X01079 Genomic DNA. Translation: CAA25555.1 .
X78993 Genomic DNA. Translation: CAA55598.1 .
Z35962 Genomic DNA. Translation: CAA85046.1 .
X01080 Genomic DNA. Translation: CAA25556.1 .
BK006936 Genomic DNA. Translation: DAA07214.1 .
PIRi S05795. PABYC.
RefSeqi NP_009651.3. NM_001178441.3.

3D structure databases

ProteinModelPortali P00635.
SMRi P00635. Positions 37-458.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32799. 37 interactions.
DIPi DIP-4916N.
IntActi P00635. 6 interactions.
MINTi MINT-478455.
STRINGi 4932.YBR093C.

Proteomic databases

MaxQBi P00635.
PaxDbi P00635.
PeptideAtlasi P00635.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR093C ; YBR093C ; YBR093C .
GeneIDi 852390.
KEGGi sce:YBR093C.

Organism-specific databases

CYGDi YBR093c.
SGDi S000000297. PHO5.

Phylogenomic databases

eggNOGi NOG260296.
GeneTreei ENSGT00510000050349.
HOGENOMi HOG000189191.
KOi K01078.
OMAi RYVINDS.
OrthoDBi EOG7TQV9N.

Enzyme and pathway databases

BioCyci YEAST:YBR093C-MONOMER.

Miscellaneous databases

NextBioi 971207.

Gene expression databases

Genevestigatori P00635.

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMi SSF53254. SSF53254. 1 hit.
PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide."
    Arima K., Oshima T., Kubota I., Nakamura N., Mizunaga T., Toh-e A.
    Nucleic Acids Res. 11:1657-1672(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-26, CATALYTIC ACTIVITY.
  2. "Structural analysis of the two tandemly repeated acid phosphatase genes in yeast."
    Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A.
    Nucleic Acids Res. 12:7721-7739(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-45.
  3. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae."
    Tait-Kamradt A.G., Turner K.J., Kramer R.A., Elliott Q.D., Bostian S.J., Thill G.P., Rogers D.T., Bostian K.A.
    Mol. Cell. Biol. 6:1855-1865(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44, CATALYTIC ACTIVITY, REPRESSION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae."
    Kennedy E.J., Pillus L., Ghosh G.
    Eukaryot. Cell 4:1892-1901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiPPA5_YEAST
AccessioniPrimary (citable) accession number: P00635
Secondary accession number(s): D6VQ94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1110 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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