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Protein

Alkaline phosphatase

Gene

phoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Magnesium1
Metal bindingi73Zinc 21
Active sitei124Phosphoserine intermediate1
Metal bindingi175Magnesium1
Metal bindingi177Magnesium1
Metal bindingi344Magnesium1
Metal bindingi349Zinc 11
Metal bindingi353Zinc 11
Metal bindingi391Zinc 21
Metal bindingi392Zinc 21
Metal bindingi434Zinc 11

GO - Molecular functioni

  • alkaline phosphatase activity Source: EcoCyc
  • hydrogenase (acceptor) activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc
  • oxidoreductase activity, acting on phosphorus or arsenic in donors Source: EcoliWiki
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • dephosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ALKAPHOSPHA-MONOMER.
ECOL316407:JW0374-MONOMER.
MetaCyc:ALKAPHOSPHA-MONOMER.
BRENDAi3.1.3.1. 2026.
SABIO-RKP00634.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
Ordered Locus Names:b0383, JW0374
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10727. phoA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002401222 – 471Alkaline phosphataseAdd BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi190 ↔ 200
Disulfide bondi308 ↔ 358

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP00634.
PRIDEiP00634.

Interactioni

Subunit structurei

Isozymes 1 and 3 are a dimer of identical chains, isozyme 2 is a dimer of heterogeneous chains, one of each of the subunits from isozymes 1 and 3.

Binary interactionsi

WithEntry#Exp.IntActNotes
dsbAP0AEG42EBI-552958,EBI-549711
LRRK2Q5S0072EBI-552958,EBI-5323863From a different organism.

Protein-protein interaction databases

BioGridi4259827. 31 interactors.
DIPiDIP-10496N.
IntActiP00634. 5 interactors.
MINTiMINT-1283619.
STRINGi511145.b0383.

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Turni16 – 18Combined sources3
Turni41 – 44Combined sources4
Helixi52 – 58Combined sources7
Beta strandi65 – 72Combined sources8
Helixi77 – 87Combined sources11
Beta strandi93 – 96Combined sources4
Helixi97 – 99Combined sources3
Beta strandi101 – 107Combined sources7
Turni113 – 115Combined sources3
Beta strandi118 – 121Combined sources4
Helixi124 – 133Combined sources10
Beta strandi142 – 144Combined sources3
Helixi154 – 160Combined sources7
Beta strandi164 – 172Combined sources9
Helixi176 – 179Combined sources4
Turni180 – 182Combined sources3
Beta strandi185 – 187Combined sources3
Helixi193 – 199Combined sources7
Helixi201 – 203Combined sources3
Helixi205 – 207Combined sources3
Helixi213 – 220Combined sources8
Beta strandi223 – 228Combined sources6
Helixi231 – 234Combined sources4
Beta strandi235 – 240Combined sources6
Turni241 – 244Combined sources4
Helixi247 – 253Combined sources7
Beta strandi257 – 259Combined sources3
Helixi262 – 267Combined sources6
Beta strandi272 – 275Combined sources4
Beta strandi277 – 280Combined sources4
Beta strandi282 – 285Combined sources4
Beta strandi289 – 291Combined sources3
Helixi296 – 298Combined sources3
Helixi299 – 302Combined sources4
Helixi312 – 314Combined sources3
Beta strandi316 – 318Combined sources3
Helixi321 – 332Combined sources12
Beta strandi339 – 345Combined sources7
Helixi347 – 353Combined sources7
Helixi357 – 381Combined sources25
Beta strandi382 – 389Combined sources8
Beta strandi391 – 393Combined sources3
Beta strandi397 – 399Combined sources3
Beta strandi401 – 403Combined sources3
Beta strandi406 – 413Combined sources8
Beta strandi417 – 424Combined sources8
Beta strandi428 – 431Combined sources4
Beta strandi433 – 435Combined sources3
Beta strandi439 – 445Combined sources7
Helixi448 – 451Combined sources4
Beta strandi452 – 456Combined sources5
Helixi457 – 467Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJAX-ray2.50A/B23-471[»]
1AJBX-ray2.50A/B23-471[»]
1AJCX-ray2.50A/B23-471[»]
1AJDX-ray2.50A/B23-471[»]
1ALHX-ray2.50A/B26-471[»]
1ALIX-ray2.20A/B23-471[»]
1ALJX-ray2.60A/B23-471[»]
1ALKX-ray2.00A/B23-471[»]
1ANIX-ray2.50A/B26-471[»]
1ANJX-ray2.30A/B26-471[»]
1B8JX-ray1.90A/B23-471[»]
1ED8X-ray1.75A/B23-471[»]
1ED9X-ray1.75A/B23-471[»]
1ELXX-ray2.60A/B23-471[»]
1ELYX-ray2.80A/B23-471[»]
1ELZX-ray2.80A/B23-471[»]
1EW8X-ray2.20A/B23-471[»]
1EW9X-ray2.00A/B23-471[»]
1HJKX-ray2.30A/B23-471[»]
1HQAX-ray2.25A/B23-471[»]
1KH4X-ray2.40A/B23-471[»]
1KH5X-ray2.00A/B23-471[»]
1KH7X-ray2.40A/B23-471[»]
1KH9X-ray2.50A/B23-471[»]
1KHJX-ray2.30A/B23-471[»]
1KHKX-ray2.50A/B23-471[»]
1KHLX-ray2.50A/B23-471[»]
1KHNX-ray2.60A/B23-471[»]
1URAX-ray2.04A/B26-471[»]
1URBX-ray2.14A/B26-471[»]
1Y6VX-ray1.60A/B23-471[»]
1Y7AX-ray1.77A/B23-471[»]
2ANHX-ray2.40A/B26-471[»]
2G9YX-ray2.00A/B23-471[»]
2GA3X-ray2.20A/B23-471[»]
2MLXNMR-B220-310[»]
2MLYNMR-B1-150[»]
2MLZNMR-B360-471[»]
3BDFX-ray1.40A/B22-471[»]
3BDGX-ray1.40A/B22-471[»]
3BDHX-ray1.85A/B22-471[»]
3CMRX-ray2.05A/B23-471[»]
3DPCX-ray2.30A/B23-471[»]
3DYCX-ray2.30A/B23-471[»]
3TG0X-ray1.20A/B/C/D23-471[»]
4KM4X-ray2.80A/B26-470[»]
4YR1X-ray2.24A/B31-471[»]
5C66X-ray2.03A/B23-471[»]
5GADelectron microscopy3.70k1-18[»]
5GAFelectron microscopy4.30k1-18[»]
5GAGelectron microscopy3.80k1-18[»]
5GAHelectron microscopy3.80k1-18[»]
5JTLNMR-E1-471[»]
5JTMNMR-E/F/G/H1-25[»]
5JTNNMR-E/F91-145[»]
5JTONMR-E/F/G/H271-310[»]
5JTPNMR-E/F/G/H450-471[»]
ProteinModelPortaliP00634.
SMRiP00634.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00634.

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099117.
InParanoidiP00634.
KOiK01077.
OMAiDITAWEY.
PhylomeDBiP00634.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSTIALAL LPLLFTPVTK ARTPEMPVLE NRAAQGDITA PGGARRLTGD
60 70 80 90 100
QTAALRDSLS DKPAKNIILL IGDGMGDSEI TAARNYAEGA GGFFKGIDAL
110 120 130 140 150
PLTGQYTHYA LNKKTGKPDY VTDSAASATA WSTGVKTYNG ALGVDIHEKD
160 170 180 190 200
HPTILEMAKA AGLATGNVST AELQDATPAA LVAHVTSRKC YGPSATSEKC
210 220 230 240 250
PGNALEKGGK GSITEQLLNA RADVTLGGGA KTFAETATAG EWQGKTLREQ
260 270 280 290 300
AQARGYQLVS DAASLNSVTE ANQQKPLLGL FADGNMPVRW LGPKATYHGN
310 320 330 340 350
IDKPAVTCTP NPQRNDSVPT LAQMTDKAIE LLSKNEKGFF LQVEGASIDK
360 370 380 390 400
QDHAANPCGQ IGETVDLDEA VQRALEFAKK EGNTLVIVTA DHAHASQIVA
410 420 430 440 450
PDTKAPGLTQ ALNTKDGAVM VMSYGNSEED SQEHTGSQLR IAAYGPHAAN
460 470
VVGLTDQTDL FYTMKAALGL K
Length:471
Mass (Da):49,439
Last modified:August 13, 1987 - v1
Checksum:i8A8DE1F29D9D9253
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → V in AAA23431 (PubMed:3912261).Curated1
Sequence conflicti78 – 80SEI → WGS in AAA24359 (PubMed:6273802).Curated3
Sequence conflicti198E → Q AA sequence (PubMed:7022451).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti22Missing in isozyme 3. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04586 Genomic DNA. Translation: CAA28257.1.
M13345 Genomic DNA. Translation: AAA83893.1.
M29664 Genomic DNA. Translation: AAA24364.1.
M29665 Genomic DNA. Translation: AAA24365.1.
U73857 Genomic DNA. Translation: AAB18107.1.
U00096 Genomic DNA. Translation: AAC73486.2.
AP009048 Genomic DNA. Translation: BAE76164.1.
M33536 Genomic DNA. Translation: AAA24372.1.
J01659 Genomic DNA. Translation: AAA24359.2.
J01660 Genomic DNA. Translation: AAA24360.1.
J01661 Genomic DNA. Translation: AAA24361.1.
J05005 Genomic DNA. Translation: AAA24362.1.
M14399 mRNA. Translation: AAA23431.1.
M13763 Genomic DNA. Translation: AAA24358.1.
PIRiA00776. PAECA.
RefSeqiNP_414917.2. NC_000913.3.
WP_000814403.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73486; AAC73486; b0383.
BAE76164; BAE76164; BAE76164.
GeneIDi945041.
KEGGiecj:JW0374.
eco:b0383.
PATRICi32115907. VBIEscCol129921_0395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04586 Genomic DNA. Translation: CAA28257.1.
M13345 Genomic DNA. Translation: AAA83893.1.
M29664 Genomic DNA. Translation: AAA24364.1.
M29665 Genomic DNA. Translation: AAA24365.1.
U73857 Genomic DNA. Translation: AAB18107.1.
U00096 Genomic DNA. Translation: AAC73486.2.
AP009048 Genomic DNA. Translation: BAE76164.1.
M33536 Genomic DNA. Translation: AAA24372.1.
J01659 Genomic DNA. Translation: AAA24359.2.
J01660 Genomic DNA. Translation: AAA24360.1.
J01661 Genomic DNA. Translation: AAA24361.1.
J05005 Genomic DNA. Translation: AAA24362.1.
M14399 mRNA. Translation: AAA23431.1.
M13763 Genomic DNA. Translation: AAA24358.1.
PIRiA00776. PAECA.
RefSeqiNP_414917.2. NC_000913.3.
WP_000814403.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJAX-ray2.50A/B23-471[»]
1AJBX-ray2.50A/B23-471[»]
1AJCX-ray2.50A/B23-471[»]
1AJDX-ray2.50A/B23-471[»]
1ALHX-ray2.50A/B26-471[»]
1ALIX-ray2.20A/B23-471[»]
1ALJX-ray2.60A/B23-471[»]
1ALKX-ray2.00A/B23-471[»]
1ANIX-ray2.50A/B26-471[»]
1ANJX-ray2.30A/B26-471[»]
1B8JX-ray1.90A/B23-471[»]
1ED8X-ray1.75A/B23-471[»]
1ED9X-ray1.75A/B23-471[»]
1ELXX-ray2.60A/B23-471[»]
1ELYX-ray2.80A/B23-471[»]
1ELZX-ray2.80A/B23-471[»]
1EW8X-ray2.20A/B23-471[»]
1EW9X-ray2.00A/B23-471[»]
1HJKX-ray2.30A/B23-471[»]
1HQAX-ray2.25A/B23-471[»]
1KH4X-ray2.40A/B23-471[»]
1KH5X-ray2.00A/B23-471[»]
1KH7X-ray2.40A/B23-471[»]
1KH9X-ray2.50A/B23-471[»]
1KHJX-ray2.30A/B23-471[»]
1KHKX-ray2.50A/B23-471[»]
1KHLX-ray2.50A/B23-471[»]
1KHNX-ray2.60A/B23-471[»]
1URAX-ray2.04A/B26-471[»]
1URBX-ray2.14A/B26-471[»]
1Y6VX-ray1.60A/B23-471[»]
1Y7AX-ray1.77A/B23-471[»]
2ANHX-ray2.40A/B26-471[»]
2G9YX-ray2.00A/B23-471[»]
2GA3X-ray2.20A/B23-471[»]
2MLXNMR-B220-310[»]
2MLYNMR-B1-150[»]
2MLZNMR-B360-471[»]
3BDFX-ray1.40A/B22-471[»]
3BDGX-ray1.40A/B22-471[»]
3BDHX-ray1.85A/B22-471[»]
3CMRX-ray2.05A/B23-471[»]
3DPCX-ray2.30A/B23-471[»]
3DYCX-ray2.30A/B23-471[»]
3TG0X-ray1.20A/B/C/D23-471[»]
4KM4X-ray2.80A/B26-470[»]
4YR1X-ray2.24A/B31-471[»]
5C66X-ray2.03A/B23-471[»]
5GADelectron microscopy3.70k1-18[»]
5GAFelectron microscopy4.30k1-18[»]
5GAGelectron microscopy3.80k1-18[»]
5GAHelectron microscopy3.80k1-18[»]
5JTLNMR-E1-471[»]
5JTMNMR-E/F/G/H1-25[»]
5JTNNMR-E/F91-145[»]
5JTONMR-E/F/G/H271-310[»]
5JTPNMR-E/F/G/H450-471[»]
ProteinModelPortaliP00634.
SMRiP00634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259827. 31 interactors.
DIPiDIP-10496N.
IntActiP00634. 5 interactors.
MINTiMINT-1283619.
STRINGi511145.b0383.

Chemistry databases

ChEMBLiCHEMBL4217.

Proteomic databases

PaxDbiP00634.
PRIDEiP00634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73486; AAC73486; b0383.
BAE76164; BAE76164; BAE76164.
GeneIDi945041.
KEGGiecj:JW0374.
eco:b0383.
PATRICi32115907. VBIEscCol129921_0395.

Organism-specific databases

EchoBASEiEB0720.
EcoGeneiEG10727. phoA.

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099117.
InParanoidiP00634.
KOiK01077.
OMAiDITAWEY.
PhylomeDBiP00634.

Enzyme and pathway databases

BioCyciEcoCyc:ALKAPHOSPHA-MONOMER.
ECOL316407:JW0374-MONOMER.
MetaCyc:ALKAPHOSPHA-MONOMER.
BRENDAi3.1.3.1. 2026.
SABIO-RKP00634.

Miscellaneous databases

EvolutionaryTraceiP00634.
PROiP00634.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_ECOLI
AccessioniPrimary (citable) accession number: P00634
Secondary accession number(s): P77801
, P78051, Q2MC42, Q47041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 180 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.