Reviewed, UniProtKB/Swiss-Prot P00633 (SAST_ANAPL)

Names and origin

Protein names	Recommended name:     S-acyl fatty acid synthase thioesterase, medium chain     EC=3.1.2.14 Alternative name(s):     Thioesterase II
Organism	Anas platyrhynchos (Domestic duck) (Anas boschas)
Taxonomic identifier	8839 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Anseriformes › Anatidae › Anas

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	In fatty acid biosynthesis chain termination and release of the free fatty acid product is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid synthetase complex. The chain length of the released fatty acid is usually C16. However, in the mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2).
Catalytic activity	Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.
Sequence similarities	Belongs to the thioesterase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oleoyl-[acyl-carrier-protein] hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	S-acyl fatty acid synthase thioesterase, medium chain		PRO_0000180357
Sites
	Active site	90	1	Probable
	Active site	226	1	By similarity
Experimental info
	Sequence conflict	43	1	V → E in AAA49219. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00633-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 3DFA25170D9EDC50	FASTA	251	28,807
	10 20 30 40 50 60 MDKVIARPYK RPNALCRLIC FPWAGGNCSF FIRWCEAFSS IIVVSVIRLA GRECRDTEPF 70 80 90 100 110 120 PEDMAEVVNE ITNALLKDLQ EKPFALFGHS FGSFVSYALA VHLKEKHGLE PVHMFFSGSY 130 140 150 160 170 180 GPHSEYFHLM YKLPEVEDSR LLELIHTLGG TPPEFLQNEQ ITKHLLRVLK EDQKVLVTYP 190 200 210 220 230 240 WHDVRKKYFS CDLTCFNGSD EKNHGSEAWI AITSGDTSIY SLPGNHFYLM EPSNETFLIK 250 YITKCIENSD I

References

[1]	"Cloning and sequencing of the cDNA for S-acyl fatty acid synthase thioesterase from the uropygial gland of mallard duck." Poulose A.J., Rogers L., Cheesbrough T.M., Kolattukudy P.E. J. Biol. Chem. 260:15953-15958(1985) [PubMed: 2415525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Uropygial gland.
[2]	"Nucleotide sequence of the S-acyl fatty acid synthase thioesterase gene and its tissue-specific expression." Sasaki G.C., Cheesbrough V., Kolattukudy P.E. DNA 7:449-457(1988) [PubMed: 2850144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Uropygial gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12101 mRNA. Translation: AAA49222.1. M21635 Genomic DNA. Translation: AAA49219.1.
PIR	ESDKTM. A00775. I50520.
3D structure databases
HSSP	HSSP built from PDB template 1MO2 based on UniProtKB Q03133.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00633.
Enzyme and pathway databases
BRENDA	3.1.2.14. 3217.
Family and domain databases
InterPro	IPR012223. TEII. IPR001031. Thioesterase. [Graphical view]
PANTHER	PTHR11487. TEII. 1 hit.
Pfam	PF00975. Thioesterase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAST_ANAPL

Accession

Primary (citable) accession number: P00633

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 20, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)