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Protein

Phospholipase A2

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi43Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi45Calcium; via carbonyl oxygenCombined sources1 Publication1
Active sitei671 Publication1
Metal bindingi68CalciumCombined sources1 Publication1
Active sitei971 Publication1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 387.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 (EC:3.1.1.4)
Short name:
bvPLA2
Alternative name(s):
Allergen Api m I
Phosphatidylcholine 2-acylhydrolase
Allergen: Api m 1
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
Proteomesi
  • UP000005203 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei2493. Api m A1-A2.
2778. Api m A1-A2-A3.
3088. Api m 1.0101.
45. Api m 1.

Chemistry databases

ChEMBLiCHEMBL4807.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002298219 – 332 PublicationsAdd BLAST15
ChainiPRO_000002298334 – 167Phospholipase A21 PublicationAdd BLAST134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 642 Publications
GlycosylationiCAR_00000146N-linked (GlcNAc...)1 Publication1
Disulfide bondi63 ↔ 1032 Publications
Disulfide bondi70 ↔ 962 Publications
Disulfide bondi94 ↔ 1282 Publications
Disulfide bondi138 ↔ 1462 Publications

Post-translational modificationi

N-glycosylated; contains mannose, N-acetylglucosamine and fucose alphal-6 and/or alphal-3 linked to the innermost N-acetylglucosamine.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP00630.

PTM databases

UniCarbKBiP00630.

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Chemistry databases

BindingDBiP00630.

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 45Combined sources6
Helixi58 – 68Combined sources11
Beta strandi71 – 74Combined sources4
Beta strandi87 – 89Combined sources3
Beta strandi91 – 93Combined sources3
Helixi94 – 105Combined sources12
Helixi110 – 122Combined sources13
Beta strandi128 – 133Combined sources6
Beta strandi135 – 137Combined sources3
Beta strandi158 – 162Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POCX-ray2.00A34-167[»]
ProteinModelPortaliP00630.
SMRiP00630.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00630.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J1W3. Eukaryota.
ENOG410YVBF. LUCA.
HOGENOMiHOG000143527.
InParanoidiP00630.
KOiK01047.
OMAiNTDISHT.
PhylomeDBiP00630.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PfamiPF05826. Phospholip_A2_2. 1 hit.
[Graphical view]
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVVLGSLFL LLLSTSHGWQ IRDRIGDNEL EERIIYPGTL WCGHGNKSSG
60 70 80 90 100
PNELGRFKHT DACCRTHDMC PDVMSAGESK HGLTNTASHT RLSCDCDDKF
110 120 130 140 150
YDCLKNSADT ISSYFVGKMY FNLIDTKCYK LEHPVTGCGE RTEGRCLHYT
160
VDKSKPKVYQ WFDLRKY
Length:167
Mass (Da):19,058
Last modified:October 25, 2002 - v3
Checksum:i88D5086A0E47DCC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72D → N AA sequence (PubMed:4448181).Curated1
Sequence conflicti85N → D AA sequence (PubMed:4448181).Curated1
Sequence conflicti89 – 90Missing AA sequence (PubMed:4448181).Curated2
Sequence conflicti95 – 99DCDDK → NNND AA sequence (PubMed:4448181).Curated5
Sequence conflicti102 – 104Missing AA sequence (PubMed:4448181).Curated3
Sequence conflicti125D → N AA sequence (PubMed:4448181).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF438408 mRNA. Translation: AAL30844.1.
EF373554 Genomic DNA. Translation: ABQ28728.1.
X16709 mRNA. Translation: CAA34681.1.
PIRiS05650. PSHBA.
RefSeqiNP_001011614.1. NM_001011614.1.
UniGeneiAme.2.

Genome annotation databases

EnsemblMetazoaiGB48228-RA; GB48228-PA; GB48228.
GeneIDi406141.
KEGGiame:406141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF438408 mRNA. Translation: AAL30844.1.
EF373554 Genomic DNA. Translation: ABQ28728.1.
X16709 mRNA. Translation: CAA34681.1.
PIRiS05650. PSHBA.
RefSeqiNP_001011614.1. NM_001011614.1.
UniGeneiAme.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POCX-ray2.00A34-167[»]
ProteinModelPortaliP00630.
SMRiP00630.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00630.
ChEMBLiCHEMBL4807.

Protein family/group databases

Allergomei2493. Api m A1-A2.
2778. Api m A1-A2-A3.
3088. Api m 1.0101.
45. Api m 1.

PTM databases

UniCarbKBiP00630.

Proteomic databases

PaxDbiP00630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiGB48228-RA; GB48228-PA; GB48228.
GeneIDi406141.
KEGGiame:406141.

Organism-specific databases

CTDi406141.

Phylogenomic databases

eggNOGiENOG410J1W3. Eukaryota.
ENOG410YVBF. LUCA.
HOGENOMiHOG000143527.
InParanoidiP00630.
KOiK01047.
OMAiNTDISHT.
PhylomeDBiP00630.

Enzyme and pathway databases

BRENDAi3.1.1.4. 387.

Miscellaneous databases

EvolutionaryTraceiP00630.
PROiP00630.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PfamiPF05826. Phospholip_A2_2. 1 hit.
[Graphical view]
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2_APIME
AccessioniPrimary (citable) accession number: P00630
Secondary accession number(s): A5JGM7, Q8WPH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 25, 2002
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The secretion of this protein into venom follows a seasonal pattern. This variation is synchronized with melittin variation, i.e. their production increase in the same months.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.