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Protein

Phospholipase A2

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Miscellaneous

The secretion of this protein into venom follows a seasonal pattern. This variation is synchronized with melittin variation, i.e. their production increase in the same months.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi43Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi45Calcium; via carbonyl oxygenCombined sources1 Publication1
Active sitei671 Publication1
Metal bindingi68CalciumCombined sources1 Publication1
Active sitei971 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4 387

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 (EC:3.1.1.4)
Short name:
bvPLA2
Alternative name(s):
Allergen Api m I
Phosphatidylcholine 2-acylhydrolase
Allergen: Api m 1
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaHymenopteraApocritaAculeataApoideaApidaeApis
Proteomesi
  • UP000005203 Componenti: Linkage group 13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei2493 Api m A1-A2
2778 Api m A1-A2-A3
3088 Api m 1.0101
45 Api m 1

Chemistry databases

ChEMBLiCHEMBL4807

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002298219 – 332 PublicationsAdd BLAST15
ChainiPRO_000002298334 – 167Phospholipase A21 PublicationAdd BLAST134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 642 Publications
GlycosylationiCAR_00000146N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi63 ↔ 1032 Publications
Disulfide bondi70 ↔ 962 Publications
Disulfide bondi94 ↔ 1282 Publications
Disulfide bondi138 ↔ 1462 Publications

Post-translational modificationi

N-glycosylated; contains mannose, N-acetylglucosamine and fucose alphal-6 and/or alphal-3 linked to the innermost N-acetylglucosamine.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP00630
PRIDEiP00630

PTM databases

GlyConnecti496
UniCarbKBiP00630

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Protein-protein interaction databases

STRINGi7460.GB13351-PA

Chemistry databases

BindingDBiP00630

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 45Combined sources6
Helixi58 – 68Combined sources11
Beta strandi71 – 74Combined sources4
Beta strandi87 – 89Combined sources3
Beta strandi91 – 93Combined sources3
Helixi94 – 105Combined sources12
Helixi110 – 122Combined sources13
Beta strandi128 – 133Combined sources6
Beta strandi135 – 137Combined sources3
Beta strandi158 – 162Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POCX-ray2.00A34-167[»]
ProteinModelPortaliP00630
SMRiP00630
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00630

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J1W3 Eukaryota
ENOG410YVBF LUCA
HOGENOMiHOG000143527
InParanoidiP00630
KOiK01047
OMAiTRLHCKC
PhylomeDBiP00630

Family and domain databases

Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PfamiView protein in Pfam
PF05826 Phospholip_A2_2, 1 hit
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVVLGSLFL LLLSTSHGWQ IRDRIGDNEL EERIIYPGTL WCGHGNKSSG
60 70 80 90 100
PNELGRFKHT DACCRTHDMC PDVMSAGESK HGLTNTASHT RLSCDCDDKF
110 120 130 140 150
YDCLKNSADT ISSYFVGKMY FNLIDTKCYK LEHPVTGCGE RTEGRCLHYT
160
VDKSKPKVYQ WFDLRKY
Length:167
Mass (Da):19,058
Last modified:October 25, 2002 - v3
Checksum:i88D5086A0E47DCC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72D → N AA sequence (PubMed:4448181).Curated1
Sequence conflicti85N → D AA sequence (PubMed:4448181).Curated1
Sequence conflicti89 – 90Missing AA sequence (PubMed:4448181).Curated2
Sequence conflicti95 – 99DCDDK → NNND AA sequence (PubMed:4448181).Curated5
Sequence conflicti102 – 104Missing AA sequence (PubMed:4448181).Curated3
Sequence conflicti125D → N AA sequence (PubMed:4448181).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF438408 mRNA Translation: AAL30844.1
EF373554 Genomic DNA Translation: ABQ28728.1
X16709 mRNA Translation: CAA34681.1
PIRiS05650 PSHBA
RefSeqiNP_001011614.1, NM_001011614.1
UniGeneiAme.2

Genome annotation databases

EnsemblMetazoaiGB48228-RA; GB48228-PA; GB48228
GeneIDi406141
KEGGiame:406141

Similar proteinsi

Entry informationi

Entry nameiPA2_APIME
AccessioniPrimary (citable) accession number: P00630
Secondary accession number(s): A5JGM7, Q8WPH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 25, 2002
Last modified: May 23, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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