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Protein

Basic phospholipase A2 notexin

Gene
N/A
Organism
Notechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Is directly toxic to skeletal muscle upon local application in vivo (dystrophic effect). Also has direct nephrotoxicity in experimental mice; a single subcutaneous dose (1.38 µg/kg) produces renal tubular and glomerular damage within 24 hours (PubMed:7580101). PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281Calcium; via carbonyl oxygenBy similarity
Metal bindingi30 – 301Calcium; via carbonyl oxygenBy similarity
Metal bindingi32 – 321Calcium; via carbonyl oxygenBy similarity
Active sitei48 – 4811 Publication
Metal bindingi49 – 491CalciumBy similarity
Active sitei93 – 9311 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Myotoxin, Neurotoxin, Presynaptic neurotoxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 notexin1 Publication (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
OrganismiNotechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Taxonomic identifieri70142 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeAcanthophiinaeNotechis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.025 mg/kg by intravenous injection.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119Basic phospholipase A2 notexinPRO_0000161675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi11 ↔ 71Combined sources1 Publication
Disulfide bondi27 ↔ 118Combined sources1 Publication
Disulfide bondi29 ↔ 45Combined sources1 Publication
Disulfide bondi44 ↔ 99Combined sources1 Publication
Disulfide bondi51 ↔ 92Combined sources1 Publication
Disulfide bondi60 ↔ 85Combined sources1 Publication
Disulfide bondi78 ↔ 90Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1211Combined sources
Turni13 – 153Combined sources
Helixi19 – 224Combined sources
Beta strandi23 – 253Combined sources
Turni26 – 283Combined sources
Beta strandi29 – 313Combined sources
Helixi40 – 5718Combined sources
Turni62 – 643Combined sources
Beta strandi69 – 724Combined sources
Beta strandi75 – 784Combined sources
Beta strandi81 – 833Combined sources
Helixi84 – 10219Combined sources
Helixi107 – 1093Combined sources
Helixi114 – 1174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AE7X-ray2.00A1-119[»]
4E4CX-ray1.80A1-119[»]
ProteinModelPortaliP00608.
SMRiP00608. Positions 1-119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00608.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NLVQFSYLIQ CANHGKRPTW HYMDYGCYCG AGGSGTPVDE LDRCCKIHDD
60 70 80 90 100
CYDEAGKKGC FPKMSAYDYY CGENGPYCRN IKKKCLRFVC DCDVEAAFCF
110
AKAPYNNANW NIDTKKRCQ
Length:119
Mass (Da):13,593
Last modified:July 21, 1986 - v1
Checksum:i23D8BF780221D852
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161K → R in variant S.

Sequence databases

PIRiA00749. PSNOAT.
S08258. PSNOAS.

Cross-referencesi

Sequence databases

PIRiA00749. PSNOAT.
S08258. PSNOAS.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AE7X-ray2.00A1-119[»]
4E4CX-ray1.80A1-119[»]
ProteinModelPortaliP00608.
SMRiP00608. Positions 1-119.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Miscellaneous databases

EvolutionaryTraceiP00608.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Amino acid sequence of a presynaptic neurotoxin from the venom of Notechis scutatus scutatus (Australian tiger snake)."
    Halpert J., Eaker D.
    J. Biol. Chem. 250:6990-6997(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Venom.
  2. "On the purification of notexin. Isolation of a single amino acid variant from the venom of Notechis scutatus scutatus."
    Chwetzoff S., Mollier P., Bouet F., Rowan E.G., Harvey A.L., Menez A.
    FEBS Lett. 261:226-230(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (NOTEXIN-S).
    Tissue: Venom.
  3. "The role of phospholipase activity in the action of a presynaptic neurotoxin from the venom of Notechis scutatus scutatus (Australian tiger snake)."
    Halpert J., Eaker D., Karlsson E.
    FEBS Lett. 61:72-76(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.
  4. "Tryptophan 110, a residue involved in the toxic activity but not in the enzymatic activity of notexin."
    Mollier P., Chwetzoff S., Bouet F., Harvey A.L., Menez A.
    Eur. J. Biochem. 185:263-270(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT OF TRP-110 IN TOXICITY.
  5. "Nephrotoxicity of notexin in experimental mice."
    Zimmerman S.E., Yong L.C.
    Exp. Toxicol. Pathol. 47:149-155(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS NEPHROTOXIN.
    Tissue: Venom.
  6. "The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0-A resolution."
    Westerlund B., Nordlund P., Uhlim U., Eaker D., Eklund H.
    FEBS Lett. 301:159-164(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), DISULFIDE BONDS.

Entry informationi

Entry nameiPA2B_NOTSC
AccessioniPrimary (citable) accession number: P00608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activity and lethal neurotoxicity are lost upon modification with p-bromophenacyl bromide.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.