Reviewed, UniProtKB/Swiss-Prot P00602 (PA21B_NAJMO)

Names and origin

Protein names	Recommended name:     Phospholipase A2 isozyme CM-I     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase
Organism	Naja mossambica (Mozambique cobra)
Taxonomic identifier	8644 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Elapinae › Naja

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Phospholipase A2 isozyme CM-I		PRO_0000161666
Sites
	Active site	47	1	By similarity
	Active site	92	1	By similarity
	Metal binding	27	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	29	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	31	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	48	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 70		By similarity
	Disulfide bond	26 ↔ 117		By similarity
	Disulfide bond	28 ↔ 44		By similarity
	Disulfide bond	43 ↔ 98		By similarity
	Disulfide bond	50 ↔ 91		By similarity
	Disulfide bond	59 ↔ 84		By similarity
	Disulfide bond	77 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00602-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A0C2DEC5EAC7C751	FASTA	118	13,219
	10 20 30 40 50 60 NLYQFKNMIH CTVPSRPWWH FADYGCYCGR GGKGTAVDDL DRCCQVHDNC YGEAEKLGCW 70 80 90 100 110 PYLTLYKYEC SQGKLTCSGG NNKCEAAVCN CDLVAANCFA GAPYIDANYN VNLKERCQ

References

[1]	"Naja mossambica mossambica venom. Purification, some properties and the amino acid sequences of three phospholipases A (CM-I, CM-II and CM-III)." Joubert F.J. Biochim. Biophys. Acta 493:216-227(1977) [PubMed: 880314] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	PSNJ1M. A00743.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00602.
Enzyme and pathway databases
BRENDA	3.1.1.4. 274614.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_NAJMO

Accession

Primary (citable) accession number: P00602

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 19, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)