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Protein

Acidic phospholipase A2 1

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that has high affinity for muscarinic acetylcholine receptors mAChRs (CHRM) and has the ability to activate them. In guinea-pig ileum, produces an onset and dose-dependent contraction. Has also weak anticoagulant activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.2 Publications

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Calcium; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi56Calcium; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi58Calcium; via carbonyl oxygenCombined sources2 Publications1
Active sitei741 Publication1
Metal bindingi75CalciumCombined sources2 Publications1
Active sitei1201 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade inhibiting toxin, G-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Hemostasis impairing toxin, Hydrolase, Neurotoxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 3558.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic phospholipase A2 1 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Muscarinic protein
Short name:
MP
Phosphatidylcholine 2-acylhydrolase
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002291622 – 272 Publications6
ChainiPRO_000002291728 – 146Acidic phospholipase A2 1Add BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 98Combined sources2 Publications
Disulfide bondi53 ↔ 145Combined sources2 Publications
Disulfide bondi55 ↔ 71Combined sources2 Publications
Disulfide bondi70 ↔ 126Combined sources2 Publications
Disulfide bondi77 ↔ 119Combined sources2 Publications
Disulfide bondi87 ↔ 112Combined sources2 Publications
Disulfide bondi105 ↔ 117Combined sources2 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 39Combined sources11
Helixi45 – 48Combined sources4
Beta strandi49 – 51Combined sources3
Turni52 – 54Combined sources3
Beta strandi55 – 57Combined sources3
Helixi66 – 81Combined sources16
Turni89 – 91Combined sources3
Beta strandi96 – 99Combined sources4
Beta strandi102 – 105Combined sources4
Helixi111 – 127Combined sources17
Helixi134 – 136Combined sources3
Helixi141 – 144Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POAX-ray1.50A28-145[»]
1POBX-ray2.00A/B28-145[»]
ProteinModelPortaliP00598.
SMRiP00598.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00598.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPAHLLILA AVCVSPLGAS SNRPMPLNLY QFKNMIQCTV PSRSWWDFAD
60 70 80 90 100
YGCYCGRGGS GTPVDDLDRC CQVHDNCYNE AEKISGCWPY FKTYSYECSQ
110 120 130 140
GTLTCKGGNN ACAAAVCDCD RLAAICFAGA PYNNNNYNID LKARCQ
Length:146
Mass (Da):16,013
Last modified:February 1, 1994 - v2
Checksum:i862EDF47654BFF93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111 – 112AC → CA AA sequence (PubMed:7222082).Curated2
Sequence conflicti134 – 136NNN → DND AA sequence (PubMed:7222082).Curated3
Sequence conflicti140D → N AA sequence (PubMed:7222082).Curated1
Sequence conflicti146Q → QE AA sequence (PubMed:7222082).Curated1

Mass spectrometryi

Molecular mass is 13260 Da from positions 28 - 146. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73225 mRNA. Translation: CAA51694.1.
PIRiJC2137. PSNJAF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73225 mRNA. Translation: CAA51694.1.
PIRiJC2137. PSNJAF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1POAX-ray1.50A28-145[»]
1POBX-ray2.00A/B28-145[»]
ProteinModelPortaliP00598.
SMRiP00598.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Enzyme and pathway databases

BRENDAi3.1.1.4. 3558.

Miscellaneous databases

EvolutionaryTraceiP00598.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2A1_NAJAT
AccessioniPrimary (citable) accession number: P00598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.