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Protein

Acidic phospholipase A2 1

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that has high affinity for muscarinic acetylcholine receptors mAChRs (CHRM) and has the ability to activate them. In guinea-pig ileum, produces an onset and dose-dependent contraction. Has also weak anticoagulant activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.2 Publications

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Calcium; via carbonyl oxygenCombined sources2 Publications
Metal bindingi56 – 561Calcium; via carbonyl oxygenCombined sources2 Publications
Metal bindingi58 – 581Calcium; via carbonyl oxygenCombined sources2 Publications
Active sitei74 – 7411 Publication
Metal bindingi75 – 751CalciumCombined sources2 Publications
Active sitei120 – 12011 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade inhibiting toxin, G-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Hemostasis impairing toxin, Hydrolase, Neurotoxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 3558.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic phospholipase A2 1 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Muscarinic protein
Short name:
MP
Phosphatidylcholine 2-acylhydrolase
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 2762 PublicationsPRO_0000022916
Chaini28 – 146119Acidic phospholipase A2 1PRO_0000022917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 98Combined sources2 Publications
Disulfide bondi53 ↔ 145Combined sources2 Publications
Disulfide bondi55 ↔ 71Combined sources2 Publications
Disulfide bondi70 ↔ 126Combined sources2 Publications
Disulfide bondi77 ↔ 119Combined sources2 Publications
Disulfide bondi87 ↔ 112Combined sources2 Publications
Disulfide bondi105 ↔ 117Combined sources2 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
146
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 3911Combined sources
Helixi45 – 484Combined sources
Beta strandi49 – 513Combined sources
Turni52 – 543Combined sources
Beta strandi55 – 573Combined sources
Helixi66 – 8116Combined sources
Turni89 – 913Combined sources
Beta strandi96 – 994Combined sources
Beta strandi102 – 1054Combined sources
Helixi111 – 12717Combined sources
Helixi134 – 1363Combined sources
Helixi141 – 1444Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1POAX-ray1.50A28-145[»]
1POBX-ray2.00A/B28-145[»]
ProteinModelPortaliP00598.
SMRiP00598. Positions 28-146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00598.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPAHLLILA AVCVSPLGAS SNRPMPLNLY QFKNMIQCTV PSRSWWDFAD
60 70 80 90 100
YGCYCGRGGS GTPVDDLDRC CQVHDNCYNE AEKISGCWPY FKTYSYECSQ
110 120 130 140
GTLTCKGGNN ACAAAVCDCD RLAAICFAGA PYNNNNYNID LKARCQ
Length:146
Mass (Da):16,013
Last modified:February 1, 1994 - v2
Checksum:i862EDF47654BFF93
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1122AC → CA AA sequence (PubMed:7222082).Curated
Sequence conflicti134 – 1363NNN → DND AA sequence (PubMed:7222082).Curated
Sequence conflicti140 – 1401D → N AA sequence (PubMed:7222082).Curated
Sequence conflicti146 – 1461Q → QE AA sequence (PubMed:7222082).Curated

Mass spectrometryi

Molecular mass is 13260 Da from positions 28 - 146. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73225 mRNA. Translation: CAA51694.1.
PIRiJC2137. PSNJAF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73225 mRNA. Translation: CAA51694.1.
PIRiJC2137. PSNJAF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1POAX-ray1.50A28-145[»]
1POBX-ray2.00A/B28-145[»]
ProteinModelPortaliP00598.
SMRiP00598. Positions 28-146.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Enzyme and pathway databases

BRENDAi3.1.1.4. 3558.

Miscellaneous databases

EvolutionaryTraceiP00598.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2A1_NAJAT
AccessioniPrimary (citable) accession number: P00598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.