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P00587 (DTX_COROM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Diphtheria toxin
Short name=DT
Alternative name(s):
NAD(+)--diphthamide ADP-ribosyltransferase
EC=2.4.2.36

Cleaved into the following 2 chains:

  1. Diphtheria toxin fragment A
  2. Diphtheria toxin fragment B
OrganismCorynephage omega
Taxonomic identifier10714 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus › unclassified Lambda-like viruses
Virus hostCorynebacterium diphtheriae [TaxID: 1717]

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existencePredicted

General annotation (Comments)

Function

Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to elongation factor 2. Fragment A is responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A.

Catalytic activity

NAD+ + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide.

Subunit structure

Homodimer.

Ontologies

Keywords
   DomainSignal
   LigandNAD
   Molecular functionGlycosyltransferase
Toxin
Transferase
   PTMCleavage on pair of basic residues
Disulfide bond
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functionNAD+-diphthamide ADP-ribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525
Chain26 – 218193Diphtheria toxin fragment A
PRO_0000019347
Chain219 – 560342Diphtheria toxin fragment B
PRO_0000019348

Sites

Active site1731
Binding site461NAD By similarity
Binding site901NAD By similarity

Amino acid modifications

Disulfide bond211 ↔ 226 By similarity
Disulfide bond486 ↔ 496 By similarity

Sequences

Sequence LengthMass (Da)Tools
P00587 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 51F5718361B94EAB

FASTA56060,815
        10         20         30         40         50         60 
MSRKLFASIL IGALLGIGAP PSAHAGADDV VDSSKSFVME NFSSYHGTKP GYVDSIQKGI 

        70         80         90        100        110        120 
QKPKSGTQGN YDDDWKGFYS TDNKYDAAGY SVDNENPLSG KAGGVVKVTY PGLTKVLALK 

       130        140        150        160        170        180 
VDNAETIKKE LGLSLTEPLM EQVGTEEFIK RFGDGASRVV LSLPFAEGSS SVEYINNWEQ 

       190        200        210        220        230        240 
AKALSVELEI NFETRGKRGQ DAMYEYMAQA CAGNRVRRSV GSSLSCINLD WDVIRDKTKT 

       250        260        270        280        290        300 
KIESLKEHGP IKNKMSESPN KTVSEEKAKQ YLEEFHQTAL EHPELSELKT VTGTNPVFAG 

       310        320        330        340        350        360 
ANYAAWAVNV AQVIDSETAD NLEKTTAALS ILPGIGSVMG IADGAVHHNT EEIVAQSIAL 

       370        380        390        400        410        420 
SSLMVAQAIP LVGELVDIGF AAYNFVESII NLFQVVHNSY NRPAYSPGHK TQPFLHDGYA 

       430        440        450        460        470        480 
VSWNTVEDSI IRTGFQGESG HDIKITAENT PLPIAGVLLP TIPGKLDVNK SKTHISVNGR 

       490        500        510        520        530        540 
KIRMRCRAID GDVTFCRPKS PVYVGNGVHA NLHVAFHRSS SEKIHSNEIS SDSIGVLGYQ 

       550        560 
KTVDHTKVNS KLSLFFEIKS

« Hide

References

[1]"The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome."
Ratti G., Rappuoli R., Giannini G.
Nucleic Acids Res. 11:6589-6595(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		V01536 Genomic DNA. Translation: CAA24778.1.
PIRDOCGPO. A00728.

3D structure databases

ProteinModelPortalP00587.
SMRP00587. Positions 26-560.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-370N.

Protein family/group databases

TCDB1.C.7.1.1. diphtheria toxin (DT) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.490.40. 1 hit.
2.60.40.700. 1 hit.
InterProIPR000512. Diphtheria_toxin.
IPR022406. Diphtheria_toxin_catalytic_dom.
IPR022404. Diphtheria_toxin_rcpt-bd_dom.
IPR022405. Diphtheria_toxin_translocation.
[Graphical view]
PfamPF02763. Diphtheria_C. 1 hit.
PF01324. Diphtheria_R. 1 hit.
PF02764. Diphtheria_T. 1 hit.
[Graphical view]
PIRSFPIRSF000490. Diphtheria_toxin. 1 hit.
PRINTSPR00769. DPTHRIATOXIN.
SUPFAMSSF49380. Diphtheria_tox. 1 hit.
SSF56845. Diphtheria_tox. 1 hit.
ProtoNetSearch...

Other

DrugBankDB00004. Denileukin diftitox.

Entry information

Entry nameDTX_COROM
AccessionPrimary (citable) accession number: P00587
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
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