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P00587

- DTX_COROM

UniProt

P00587 - DTX_COROM

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Protein
Diphtheria toxin
Gene
N/A
Organism
Corynephage omega
Status
Reviewed - Annotation score: 4 out of 5 - Protein predictedi

Functioni

Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to elongation factor 2. Fragment A is responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A.

Catalytic activityi

NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461NAD By similarity
Binding sitei90 – 901NAD By similarity
Active sitei173 – 1731

GO - Molecular functioni

  1. NAD+-diphthamide ADP-ribosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Toxin, Transferase

Keywords - Ligandi

NAD

Protein family/group databases

TCDBi1.C.7.1.1. the diphtheria toxin (dt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphtheria toxin
Short name:
DT
Alternative name(s):
NAD(+)--diphthamide ADP-ribosyltransferase (EC:2.4.2.36)
Cleaved into the following 2 chains:
OrganismiCorynephage omega
Taxonomic identifieri10714 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostiCorynebacterium diphtheriae [TaxID: 1717]

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525
Add
BLAST
Chaini26 – 218193Diphtheria toxin fragment A
PRO_0000019347Add
BLAST
Chaini219 – 560342Diphtheria toxin fragment B
PRO_0000019348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi211 ↔ 226 By similarity
Disulfide bondi486 ↔ 496 By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-370N.
MINTiMINT-1536121.

Structurei

3D structure databases

ProteinModelPortaliP00587.
SMRiP00587. Positions 26-560.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.490.40. 1 hit.
2.60.40.700. 1 hit.
InterProiIPR000512. Diphtheria_toxin.
IPR022406. Diphtheria_toxin_catalytic_dom.
IPR022404. Diphtheria_toxin_rcpt-bd_dom.
IPR022405. Diphtheria_toxin_translocation.
[Graphical view]
PfamiPF02763. Diphtheria_C. 1 hit.
PF01324. Diphtheria_R. 1 hit.
PF02764. Diphtheria_T. 1 hit.
[Graphical view]
PIRSFiPIRSF000490. Diphtheria_toxin. 1 hit.
PRINTSiPR00769. DPTHRIATOXIN.
SUPFAMiSSF49380. SSF49380. 1 hit.
SSF56845. SSF56845. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00587-1 [UniParc]FASTAAdd to Basket

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MSRKLFASIL IGALLGIGAP PSAHAGADDV VDSSKSFVME NFSSYHGTKP    50
GYVDSIQKGI QKPKSGTQGN YDDDWKGFYS TDNKYDAAGY SVDNENPLSG 100
KAGGVVKVTY PGLTKVLALK VDNAETIKKE LGLSLTEPLM EQVGTEEFIK 150
RFGDGASRVV LSLPFAEGSS SVEYINNWEQ AKALSVELEI NFETRGKRGQ 200
DAMYEYMAQA CAGNRVRRSV GSSLSCINLD WDVIRDKTKT KIESLKEHGP 250
IKNKMSESPN KTVSEEKAKQ YLEEFHQTAL EHPELSELKT VTGTNPVFAG 300
ANYAAWAVNV AQVIDSETAD NLEKTTAALS ILPGIGSVMG IADGAVHHNT 350
EEIVAQSIAL SSLMVAQAIP LVGELVDIGF AAYNFVESII NLFQVVHNSY 400
NRPAYSPGHK TQPFLHDGYA VSWNTVEDSI IRTGFQGESG HDIKITAENT 450
PLPIAGVLLP TIPGKLDVNK SKTHISVNGR KIRMRCRAID GDVTFCRPKS 500
PVYVGNGVHA NLHVAFHRSS SEKIHSNEIS SDSIGVLGYQ KTVDHTKVNS 550
KLSLFFEIKS 560
Length:560
Mass (Da):60,815
Last modified:July 21, 1986 - v1
Checksum:i51F5718361B94EAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01536 Genomic DNA. Translation: CAA24778.1.
PIRiA00728. DOCGPO.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01536 Genomic DNA. Translation: CAA24778.1 .
PIRi A00728. DOCGPO.

3D structure databases

ProteinModelPortali P00587.
SMRi P00587. Positions 26-560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-370N.
MINTi MINT-1536121.

Chemistry

DrugBanki DB00004. Denileukin diftitox.

Protein family/group databases

TCDBi 1.C.7.1.1. the diphtheria toxin (dt) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.490.40. 1 hit.
2.60.40.700. 1 hit.
InterProi IPR000512. Diphtheria_toxin.
IPR022406. Diphtheria_toxin_catalytic_dom.
IPR022404. Diphtheria_toxin_rcpt-bd_dom.
IPR022405. Diphtheria_toxin_translocation.
[Graphical view ]
Pfami PF02763. Diphtheria_C. 1 hit.
PF01324. Diphtheria_R. 1 hit.
PF02764. Diphtheria_T. 1 hit.
[Graphical view ]
PIRSFi PIRSF000490. Diphtheria_toxin. 1 hit.
PRINTSi PR00769. DPTHRIATOXIN.
SUPFAMi SSF49380. SSF49380. 1 hit.
SSF56845. SSF56845. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome."
    Ratti G., Rappuoli R., Giannini G.
    Nucleic Acids Res. 11:6589-6595(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDTX_COROM
AccessioniPrimary (citable) accession number: P00587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

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