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P00587

- DTX_COROM

UniProt

P00587 - DTX_COROM

Protein

Diphtheria toxin

Gene
N/A
Organism
Corynephage omega
Status
Reviewed - Annotation score: 3 out of 5- Protein predictedi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to elongation factor 2. Fragment A is responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A.

    Catalytic activityi

    NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei46 – 461NADBy similarity
    Binding sitei90 – 901NADBy similarity
    Active sitei173 – 1731

    GO - Molecular functioni

    1. NAD+-diphthamide ADP-ribosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Toxin, Transferase

    Keywords - Ligandi

    NAD

    Protein family/group databases

    TCDBi1.C.7.1.1. the diphtheria toxin (dt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diphtheria toxin
    Short name:
    DT
    Alternative name(s):
    NAD(+)--diphthamide ADP-ribosyltransferase (EC:2.4.2.36)
    Cleaved into the following 2 chains:
    OrganismiCorynephage omega
    Taxonomic identifieri10714 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
    Virus hostiCorynebacterium diphtheriae [TaxID: 1717]

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Add
    BLAST
    Chaini26 – 218193Diphtheria toxin fragment APRO_0000019347Add
    BLAST
    Chaini219 – 560342Diphtheria toxin fragment BPRO_0000019348Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi211 ↔ 226By similarity
    Disulfide bondi486 ↔ 496By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-370N.
    MINTiMINT-1536121.

    Structurei

    3D structure databases

    ProteinModelPortaliP00587.
    SMRiP00587. Positions 26-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.10.490.40. 1 hit.
    2.60.40.700. 1 hit.
    InterProiIPR000512. Diphtheria_toxin.
    IPR022406. Diphtheria_toxin_catalytic_dom.
    IPR022404. Diphtheria_toxin_rcpt-bd_dom.
    IPR022405. Diphtheria_toxin_translocation.
    [Graphical view]
    PfamiPF02763. Diphtheria_C. 1 hit.
    PF01324. Diphtheria_R. 1 hit.
    PF02764. Diphtheria_T. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000490. Diphtheria_toxin. 1 hit.
    PRINTSiPR00769. DPTHRIATOXIN.
    SUPFAMiSSF49380. SSF49380. 1 hit.
    SSF56845. SSF56845. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00587-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRKLFASIL IGALLGIGAP PSAHAGADDV VDSSKSFVME NFSSYHGTKP    50
    GYVDSIQKGI QKPKSGTQGN YDDDWKGFYS TDNKYDAAGY SVDNENPLSG 100
    KAGGVVKVTY PGLTKVLALK VDNAETIKKE LGLSLTEPLM EQVGTEEFIK 150
    RFGDGASRVV LSLPFAEGSS SVEYINNWEQ AKALSVELEI NFETRGKRGQ 200
    DAMYEYMAQA CAGNRVRRSV GSSLSCINLD WDVIRDKTKT KIESLKEHGP 250
    IKNKMSESPN KTVSEEKAKQ YLEEFHQTAL EHPELSELKT VTGTNPVFAG 300
    ANYAAWAVNV AQVIDSETAD NLEKTTAALS ILPGIGSVMG IADGAVHHNT 350
    EEIVAQSIAL SSLMVAQAIP LVGELVDIGF AAYNFVESII NLFQVVHNSY 400
    NRPAYSPGHK TQPFLHDGYA VSWNTVEDSI IRTGFQGESG HDIKITAENT 450
    PLPIAGVLLP TIPGKLDVNK SKTHISVNGR KIRMRCRAID GDVTFCRPKS 500
    PVYVGNGVHA NLHVAFHRSS SEKIHSNEIS SDSIGVLGYQ KTVDHTKVNS 550
    KLSLFFEIKS 560
    Length:560
    Mass (Da):60,815
    Last modified:July 21, 1986 - v1
    Checksum:i51F5718361B94EAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01536 Genomic DNA. Translation: CAA24778.1.
    PIRiA00728. DOCGPO.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01536 Genomic DNA. Translation: CAA24778.1 .
    PIRi A00728. DOCGPO.

    3D structure databases

    ProteinModelPortali P00587.
    SMRi P00587. Positions 26-560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-370N.
    MINTi MINT-1536121.

    Chemistry

    DrugBanki DB00004. Denileukin diftitox.

    Protein family/group databases

    TCDBi 1.C.7.1.1. the diphtheria toxin (dt) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.490.40. 1 hit.
    2.60.40.700. 1 hit.
    InterProi IPR000512. Diphtheria_toxin.
    IPR022406. Diphtheria_toxin_catalytic_dom.
    IPR022404. Diphtheria_toxin_rcpt-bd_dom.
    IPR022405. Diphtheria_toxin_translocation.
    [Graphical view ]
    Pfami PF02763. Diphtheria_C. 1 hit.
    PF01324. Diphtheria_R. 1 hit.
    PF02764. Diphtheria_T. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000490. Diphtheria_toxin. 1 hit.
    PRINTSi PR00769. DPTHRIATOXIN.
    SUPFAMi SSF49380. SSF49380. 1 hit.
    SSF56845. SSF56845. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome."
      Ratti G., Rappuoli R., Giannini G.
      Nucleic Acids Res. 11:6589-6595(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDTX_COROM
    AccessioniPrimary (citable) accession number: P00587
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program