P00585 (RDRP_BPMS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: RNA-directed RNA polymerase beta chain EC=2.7.7.48 Alternative name(s): RNA replicase beta chain
Organism	Enterobacteria phage MS2 (Bacteriophage MS2) [Reference proteome]
Taxonomic identifier	329852 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Leviviridae › Levivirus
Virus host	Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme is part of the viral RNA-dependent RNA polymerase complex.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	The polymerase complex is composed of four chains, the three other proteins of the complex (alpha, gamma, and delta chains) are supplied by the host cell.
Miscellaneous	This is the first protein for which the amino acid sequence has been entirely deduced from the nucleotide sequence data.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host
	Chain	2 – 545	544	RNA-directed RNA polymerase beta chain		PRO_0000164854
Regions
	Domain	243 – 373	131	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P00585 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A723402D43CD9904	FASTA	545	60,837
	10 20 30 40 50 60 MSKTTKKFNS LCIDLPRDLS LEIYQSIASV ATGSGDPHSD DFTAIAYLRD ELLTKHPTLG 70 80 90 100 110 120 SGNDEATRRT LAIAKLREAN GDRGQINREG FLHDKSLSWD PDVLQTSIRS LIGNLLSGYR 130 140 150 160 170 180 SSLFGQCTFS NGAPMGHKLQ DAAPYKKFAE QATVTPRALR AALLVRDQCA PWIRHAVRYN 190 200 210 220 230 240 ESYEFRLVVG NGVFTVPKNN KIDRAACKEP DMNMYLQKGV GAFIRRRLKS VGIDLNDQSI 250 260 270 280 290 300 NQRLAQQGSV DGSLATIDLS SASDSISDRL VWSFLPPELY SYLDRIRSHY GIVDGETIRW 310 320 330 340 350 360 ELFSTMGNGF TFELESMIFW AIVKATQIHF GNAGTIGIYG DDIICPSEIA PRVLEALAYY 370 380 390 400 410 420 GFKPNLRKTF VSGLFRESCG AHFYRGVDVK PFYIKKPVDN LFALMLILNR LRGWGVVGGM 430 440 450 460 470 480 SDPRLYKVWV RLSSQVPSMF FGGTDLAADY YVVSPPTAVS VYTKTPYGRL LADTRTSGFR 490 500 510 520 530 540 LARIARERKF FSEKHDSGRY IAWFHTGGEI TDSMKSAGVR VIRTSEWLTP VPTFPQECGP ASSPR

References

[1]	"Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene." Fiers W., Contreras R., Duerinck F., Haegeman G., Iserentant D., Merregaert J., Min Jou W., Molemans F., Raeymaekers A., van den Berghe A., Volckaert G., Ysebaert M. Nature 260:500-507(1976) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Studies on the bacteriophage MS2. XLI. Nature of the azure mutation." Iserentant D., van Montagu M., Fiers W. J. Mol. Biol. 139:243-263(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 488-545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00642 mRNA. Translation: CAA23991.1. M10565 Genomic RNA. Translation: AAA32263.1.
PIR	RRBPBM. A00723.
RefSeq	NP_040650.1. NC_001417.2.
3D structure databases
ProteinModelPortal	P00585.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1260900.
Phylogenomic databases
ProtClustDB	PHA0028.
Family and domain databases
InterPro	IPR007096. RNA-dir_Rpol_phage_catalytic. IPR005093. RNArep_beta. [Graphical view]
Pfam	PF03431. RNA_replicase_B. 1 hit. [Graphical view]
PROSITE	PS50522. RDRP_PHAGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_BPMS2

Accession

Primary (citable) accession number: P00585

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program