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Protein

DNA polymerase I

Gene

polA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • 3'-5' exonuclease activity Source: EcoCyc
  • 5'-3' exonuclease activity Source: EcoCyc
  • DNA binding Source: EcoCyc
  • DNA-directed DNA polymerase activity Source: EcoCyc

GO - Biological processi

  • base-excision repair Source: EcoCyc
  • DNA-dependent DNA replication Source: EcoCyc
  • DNA repair Source: EcoCyc
  • DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10746-MONOMER.
ECOL316407:JW3835-MONOMER.
MetaCyc:EG10746-MONOMER.
BRENDAi2.7.7.7. 2026.
SABIO-RKP00582.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Synonyms:resA
Ordered Locus Names:b3863, JW3835
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10746. polA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4298.
DrugBankiDB00548. Azelaic Acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012391 – 928DNA polymerase IAdd BLAST928

Proteomic databases

EPDiP00582.
PaxDbiP00582.
PRIDEiP00582.

2D gel databases

SWISS-2DPAGEP00582.

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Protein-protein interaction databases

BioGridi4262182. 113 interactors.
DIPiDIP-10524N.
IntActiP00582. 28 interactors.
MINTiMINT-1225247.
STRINGi511145.b3863.

Chemistry databases

BindingDBiP00582.

Structurei

Secondary structure

1928
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi327 – 332Combined sources6
Helixi336 – 347Combined sources12
Beta strandi349 – 359Combined sources11
Turni363 – 365Combined sources3
Beta strandi368 – 376Combined sources9
Beta strandi379 – 384Combined sources6
Helixi398 – 409Combined sources12
Beta strandi416 – 420Combined sources5
Helixi421 – 429Combined sources9
Turni430 – 432Combined sources3
Beta strandi438 – 441Combined sources4
Helixi442 – 449Combined sources8
Helixi451 – 453Combined sources3
Helixi458 – 465Combined sources8
Helixi473 – 477Combined sources5
Helixi480 – 482Combined sources3
Helixi486 – 488Combined sources3
Helixi491 – 515Combined sources25
Helixi520 – 528Combined sources9
Helixi530 – 543Combined sources14
Beta strandi545 – 547Combined sources3
Helixi549 – 573Combined sources25
Beta strandi574 – 576Combined sources3
Turni582 – 585Combined sources4
Helixi587 – 590Combined sources4
Turni591 – 593Combined sources3
Turni608 – 610Combined sources3
Helixi613 – 617Combined sources5
Turni618 – 620Combined sources3
Helixi623 – 639Combined sources17
Turni640 – 643Combined sources4
Helixi644 – 647Combined sources4
Turni650 – 652Combined sources3
Beta strandi653 – 655Combined sources3
Beta strandi658 – 662Combined sources5
Beta strandi665 – 667Combined sources3
Beta strandi670 – 674Combined sources5
Helixi676 – 678Combined sources3
Beta strandi681 – 683Combined sources3
Helixi684 – 691Combined sources8
Beta strandi699 – 706Combined sources8
Helixi709 – 717Combined sources9
Helixi721 – 728Combined sources8
Helixi733 – 741Combined sources9
Helixi746 – 748Combined sources3
Helixi751 – 765Combined sources15
Helixi772 – 777Combined sources6
Turni781 – 783Combined sources3
Helixi784 – 794Combined sources11
Helixi796 – 812Combined sources17
Beta strandi813 – 816Combined sources4
Beta strandi822 – 824Combined sources3
Turni826 – 829Combined sources4
Helixi833 – 870Combined sources38
Beta strandi873 – 880Combined sources8
Beta strandi883 – 889Combined sources7
Turni890 – 892Combined sources3
Helixi893 – 906Combined sources14
Beta strandi910 – 912Combined sources3
Beta strandi916 – 923Combined sources8
Helixi924 – 927Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8YX-ray2.08A324-928[»]
1D9DX-ray2.18A324-928[»]
1D9FX-ray3.00A324-928[»]
1DPIX-ray2.80A324-928[»]
1KFDX-ray3.90A324-928[»]
1KFSX-ray2.10A324-928[»]
1KLNX-ray3.20A324-928[»]
1KRPX-ray2.20A324-928[»]
1KSPX-ray2.30A324-928[»]
1QSLX-ray2.20A324-928[»]
2KFNX-ray2.03A324-928[»]
2KFZX-ray2.03A324-928[»]
2KZMX-ray2.60A324-928[»]
2KZZX-ray2.25A324-928[»]
ProteinModelPortaliP00582.
SMRiP00582.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00582.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 3235'-3' exonucleaseAdd BLAST323
Domaini324 – 5173'-5' exonucleaseAdd BLAST194

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni324 – 928Klenow fragmentAdd BLAST605
Regioni521 – 928PolymeraseAdd BLAST408

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 5'-3' exonuclease domain.Curated

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
InParanoidiP00582.
KOiK02335.
OMAiCFDTETT.
PhylomeDBiP00582.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQIPQNPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI
60 70 80 90 100
MQYKPTHAAV VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK
110 120 130 140 150
AMGLPLLAVS GVEADDVIGT LAREAEKAGR PVLISTGDKD MAQLVTPNIT
160 170 180 190 200
LINTMTNTIL GPEEVVNKYG VPPELIIDFL ALMGDSSDNI PGVPGVGEKT
210 220 230 240 250
AQALLQGLGG LDTLYAEPEK IAGLSFRGAK TMAAKLEQNK EVAYLSYQLA
260 270 280 290 300
TIKTDVELEL TCEQLEVQQP AAEELLGLFK KYEFKRWTAD VEAGKWLQAK
310 320 330 340 350
GAKPAAKPQE TSVADEAPEV TATVISYDNY VTILDEETLK AWIAKLEKAP
360 370 380 390 400
VFAFDTETDS LDNISANLVG LSFAIEPGVA AYIPVAHDYL DAPDQISRER
410 420 430 440 450
ALELLKPLLE DEKALKVGQN LKYDRGILAN YGIELRGIAF DTMLESYILN
460 470 480 490 500
SVAGRHDMDS LAERWLKHKT ITFEEIAGKG KNQLTFNQIA LEEAGRYAAE
510 520 530 540 550
DADVTLQLHL KMWPDLQKHK GPLNVFENIE MPLVPVLSRI ERNGVKIDPK
560 570 580 590 600
VLHNHSEELT LRLAELEKKA HEIAGEEFNL SSTKQLQTIL FEKQGIKPLK
610 620 630 640 650
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP
660 670 680 690 700
KTGRVHTSYH QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV
710 720 730 740 750
IVSADYSQIE LRIMAHLSRD KGLLTAFAEG KDIHRATAAE VFGLPLETVT
760 770 780 790 800
SEQRRSAKAI NFGLIYGMSA FGLARQLNIP RKEAQKYMDL YFERYPGVLE
810 820 830 840 850
YMERTRAQAK EQGYVETLDG RRLYLPDIKS SNGARRAAAE RAAINAPMQG
860 870 880 890 900
TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDVDAVAKQI
910 920
HQLMENCTRL DVPLLVEVGS GENWDQAH
Length:928
Mass (Da):103,118
Last modified:July 21, 1986 - v1
Checksum:iDAAE1C448A59030C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00317 Genomic DNA. Translation: CAA23607.1.
L19201 Genomic DNA. Translation: AAB02998.1.
U00096 Genomic DNA. Translation: AAC76861.1.
AP009048 Genomic DNA. Translation: BAE77445.1.
J01663 Genomic DNA. Translation: AAA24402.1.
J01664 Genomic DNA. Translation: AAA24404.1.
PIRiA92360. DJECI.
RefSeqiNP_418300.1. NC_000913.3.
WP_000250006.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76861; AAC76861; b3863.
BAE77445; BAE77445; BAE77445.
GeneIDi948356.
KEGGiecj:JW3835.
eco:b3863.
PATRICi32123225. VBIEscCol129921_3973.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00317 Genomic DNA. Translation: CAA23607.1.
L19201 Genomic DNA. Translation: AAB02998.1.
U00096 Genomic DNA. Translation: AAC76861.1.
AP009048 Genomic DNA. Translation: BAE77445.1.
J01663 Genomic DNA. Translation: AAA24402.1.
J01664 Genomic DNA. Translation: AAA24404.1.
PIRiA92360. DJECI.
RefSeqiNP_418300.1. NC_000913.3.
WP_000250006.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8YX-ray2.08A324-928[»]
1D9DX-ray2.18A324-928[»]
1D9FX-ray3.00A324-928[»]
1DPIX-ray2.80A324-928[»]
1KFDX-ray3.90A324-928[»]
1KFSX-ray2.10A324-928[»]
1KLNX-ray3.20A324-928[»]
1KRPX-ray2.20A324-928[»]
1KSPX-ray2.30A324-928[»]
1QSLX-ray2.20A324-928[»]
2KFNX-ray2.03A324-928[»]
2KFZX-ray2.03A324-928[»]
2KZMX-ray2.60A324-928[»]
2KZZX-ray2.25A324-928[»]
ProteinModelPortaliP00582.
SMRiP00582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262182. 113 interactors.
DIPiDIP-10524N.
IntActiP00582. 28 interactors.
MINTiMINT-1225247.
STRINGi511145.b3863.

Chemistry databases

BindingDBiP00582.
ChEMBLiCHEMBL4298.
DrugBankiDB00548. Azelaic Acid.

2D gel databases

SWISS-2DPAGEP00582.

Proteomic databases

EPDiP00582.
PaxDbiP00582.
PRIDEiP00582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76861; AAC76861; b3863.
BAE77445; BAE77445; BAE77445.
GeneIDi948356.
KEGGiecj:JW3835.
eco:b3863.
PATRICi32123225. VBIEscCol129921_3973.

Organism-specific databases

EchoBASEiEB0739.
EcoGeneiEG10746. polA.

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
InParanoidiP00582.
KOiK02335.
OMAiCFDTETT.
PhylomeDBiP00582.

Enzyme and pathway databases

BioCyciEcoCyc:EG10746-MONOMER.
ECOL316407:JW3835-MONOMER.
MetaCyc:EG10746-MONOMER.
BRENDAi2.7.7.7. 2026.
SABIO-RKP00582.

Miscellaneous databases

EvolutionaryTraceiP00582.
PROiP00582.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
PF01612. DNA_pol_A_exo1. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_ECOLI
AccessioniPrimary (citable) accession number: P00582
Secondary accession number(s): Q2M8G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 180 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.