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P00581 (DPOL_BPT7) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase
EC=2.7.7.7
Alternative name(s):
T7 DNA polymerase
Gene names
Name:5
OrganismEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifier10760 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

Composed of two subunits. One is encoded by the phage and the other is encoded by the host thioredoxin.

Sequence similarities

Belongs to the DNA polymerase type-A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 704704DNA polymerase
PRO_0000101269

Secondary structure

1 ...................................................................................................... 704
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00581 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 17089CE2AD9FB596

FASTA70479,692
        10         20         30         40         50         60 
MIVSDIEANA LLESVTKFHC GVIYDYSTAE YVSYRPSDFG AYLDALEAEV ARGGLIVFHN 

        70         80         90        100        110        120 
GHKYDVPALT KLAKLQLNRE FHLPRENCID TLVLSRLIHS NLKDTDMGLL RSGKLPGKRF 

       130        140        150        160        170        180 
GSHALEAWGY RLGEMKGEYK DDFKRMLEEQ GEEYVDGMEW WNFNEEMMDY NVQDVVVTKA 

       190        200        210        220        230        240 
LLEKLLSDKH YFPPEIDFTD VGYTTFWSES LEAVDIEHRA AWLLAKQERN GFPFDTKAIE 

       250        260        270        280        290        300 
ELYVELAARR SELLRKLTET FGSWYQPKGG TEMFCHPRTG KPLPKYPRIK TPKVGGIFKK 

       310        320        330        340        350        360 
PKNKAQREGR EPCELDTREY VAGAPYTPVE HVVFNPSSRD HIQKKLQEAG WVPTKYTDKG 

       370        380        390        400        410        420 
APVVDDEVLE GVRVDDPEKQ AAIDLIKEYL MIQKRIGQSA EGDKAWLRYV AEDGKIHGSV 

       430        440        450        460        470        480 
NPNGAVTGRA THAFPNLAQI PGVRSPYGEQ CRAAFGAEHH LDGITGKPWV QAGIDASGLE 

       490        500        510        520        530        540 
LRCLAHFMAR FDNGEYAHEI LNGDIHTKNQ IAAELPTRDN AKTFIYGFLY GAGDEKIGQI 

       550        560        570        580        590        600 
VGAGKERGKE LKKKFLENTP AIAALRESIQ QTLVESSQWV AGEQQVKWKR RWIKGLDGRK 

       610        620        630        640        650        660 
VHVRSPHAAL NTLLQSAGAL ICKLWIIKTE EMLVEKGLKH GWDGDFAYMA WVHDEIQVGC 

       670        680        690        700 
RTEEIAQVVI ETAQEAMRWV GDHWNFRCLL DTEGKMGPNW AICH

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements."
Dunn J.J., Studier F.W.
J. Mol. Biol. 166:477-535(1983) [PubMed: 6864790] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase."
Moffatt B.A., Dunn J.J., Studier F.W.
J. Mol. Biol. 173:265-269(1984) [PubMed: 6708104] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Crystal structure of a bacteriophage T7 DNA replication complex at 2.2-A resolution."
Doublie S., Tabor S., Long A.M., Richardson C.C., Ellenberger T.
Nature 391:251-258(1998) [PubMed: 9440688] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		V01146 Genomic DNA. Translation: CAA24412.1.
PIRDJBPT7. A00716.
RefSeqNP_041982.1. NC_001604.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SKRX-ray2.40A1-704[»]
1SKSX-ray2.30A1-704[»]
1SKWX-ray2.30A1-704[»]
1SL0X-ray3.20A/C1-704[»]
1SL1X-ray2.20A1-704[»]
1SL2X-ray2.30A1-704[»]
1T7PX-ray2.20A1-704[»]
1T8EX-ray2.54A1-704[»]
1TK0X-ray2.30A1-704[»]
1TK5X-ray2.20A1-704[»]
1TK8X-ray2.50A1-704[»]
1TKDX-ray2.49A1-704[»]
1X9MX-ray2.10A1-704[»]
1X9SX-ray2.70A1-704[»]
1X9WX-ray2.30A1-704[»]
1ZYQX-ray2.70A1-698[»]
2AJQX-ray2.60A/F1-704[»]
ProteinModelPortalP00581.
SMRP00581. Positions 1-704.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1261044.

Phylogenomic databases

ProtClustDBCLSP2343213.

Family and domain databases

InterProIPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSPR00868. DNAPOLI.
SMARTSM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMSSF53098. RNaseH_fold. 1 hit.
PROSITEPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_BPT7
AccessionPrimary (citable) accession number: P00581
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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