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Reviewed, UniProtKB/Swiss-Prot P00573 (RPOL_BPT7)

Last modified June 16, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA-directed RNA polymerase
    EC=2.7.7.6
Gene names
Name: 1
OrganismEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifier10760 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses
Virus hostEscherichia coli [TaxID: 562]

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Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Monomer.

Sequence similarities

Belongs to the phage and mitochondrial RNA polymerase family.

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Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processtranscription

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding Ref.12

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

3.5P008061EBI-1026948,EBI-1026942

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 883883DNA-directed RNA polymerase
PRO_0000087749

Sites

Active site5371
Active site6311 Ref.6
Active site8121

Experimental info

Mutagenesis1721K → L or G: No change in activity. Ref.7
Mutagenesis5631P → A or T: Inactivated.
Mutagenesis5711Y → S: Inactivated.
Mutagenesis6311K → G: Partially inactivated. Ref.6
Mutagenesis6311K → L: Partially inactivated. Ref.6
Mutagenesis6311K → R: Partially inactivated. Ref.6
Mutagenesis6361T → P: Inactivated.
Mutagenesis6391Y → D: Inactivated.
Mutagenesis6461F → C: Inactivated.
Sequence conflict388 – 42437DKARK…MDWRG → TRLASLAVSALSSCLSKPIS LLTIRPSGSLTTWTGAV Ref.3
Sequence conflict3891K → R in AAA32569. Ref.4
Sequence conflict4431L → R in CAA24333. Ref.3
Sequence conflict4741P → S in CAA24333. Ref.3
Sequence conflict6231Y → H in AAA32569. Ref.4
Sequence conflict6651L → P in AAA32569. Ref.4

Secondary structure

1 ................................................................................................................................... 883
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P00573-1 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 7FBDEAC457842549

FASTA88398,855
        10         20         30         40         50         60 
MNTINIAKND FSDIELAAIP FNTLADHYGE RLAREQLALE HESYEMGEAR FRKMFERQLK 

        70         80         90        100        110        120 
AGEVADNAAA KPLITTLLPK MIARINDWFE EVKAKRGKRP TAFQFLQEIK PEAVAYITIK 

       130        140        150        160        170        180 
TTLACLTSAD NTTVQAVASA IGRAIEDEAR FGRIRDLEAK HFKKNVEEQL NKRVGHVYKK 

       190        200        210        220        230        240 
AFMQVVEADM LSKGLLGGEA WSSWHKEDSI HVGVRCIEML IESTGMVSLH RQNAGVVGQD 

       250        260        270        280        290        300 
SETIELAPEY AEAIATRAGA LAGISPMFQP CVVPPKPWTG ITGGGYWANG RRPLALVRTH 

       310        320        330        340        350        360 
SKKALMRYED VYMPEVYKAI NIAQNTAWKI NKKVLAVANV ITKWKHCPVE DIPAIEREEL 

       370        380        390        400        410        420 
PMKPEDIDMN PEALTAWKRA AAAVYRKDKA RKSRRISLEF MLEQANKFAN HKAIWFPYNM 

       430        440        450        460        470        480 
DWRGRVYAVS MFNPQGNDMT KGLLTLAKGK PIGKEGYYWL KIHGANCAGV DKVPFPERIK 

       490        500        510        520        530        540 
FIEENHENIM ACAKSPLENT WWAEQDSPFC FLAFCFEYAG VQHHGLSYNC SLPLAFDGSC 

       550        560        570        580        590        600 
SGIQHFSAML RDEVGGRAVN LLPSETVQDI YGIVAKKVNE ILQADAINGT DNEVVTVTDE 

       610        620        630        640        650        660 
NTGEISEKVK LGTKALAGQW LAYGVTRSVT KRSVMTLAYG SKEFGFRQQV LEDTIQPAID 

       670        680        690        700        710        720 
SGKGLMFTQP NQAAGYMAKL IWESVSVTVV AAVEAMNWLK SAAKLLAAEV KDKKTGEILR 

       730        740        750        760        770        780 
KRCAVHWVTP DGFPVWQEYK KPIQTRLNLM FLGQFRLQPT INTNKDSEID AHKQESGIAP 

       790        800        810        820        830        840 
NFVHSQDGSH LRKTVVWAHE KYGIESFALI HDSFGTIPAD AANLFKAVRE TMVDTYESCD 

       850        860        870        880 
VLADFYDQFA DQLHESQLDK MPALPAKGNL NLRDILESDF AFA

« Hide

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References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements."
Dunn J.J., Studier F.W.
J. Mol. Biol. 166:477-535(1983) [PubMed: 6864790] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase."
Moffatt B.A., Dunn J.J., Studier F.W.
J. Mol. Biol. 173:265-269(1984) [PubMed: 6708104] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymerase."
Stahl S.J., Zinn K.
J. Mol. Biol. 148:481-485(1981) [PubMed: 7310873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Phage T7 RNA-polymerase: gene cloning and its structure."
Grachev M.A., Pletnev A.G.
Bioorg. Khim. 10:824-843(1984) [PubMed: 6093820] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4."
Dunn J.J., Studier F.W.
J. Mol. Biol. 148:303-330(1981) [PubMed: 7310871] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59 AND 829-883.
[6]"Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesis."
Maksimova T.G., Mustayev A.A., Zaychikov E.F., Lyakhov D.L., Tunitskaya V.L., Akbarov A.K., Luchin S.V., Rechinsky V.O., Chernov B.K., Kochetkov S.N.
Eur. J. Biochem. 195:841-847(1991) [PubMed: 1847871] [Abstract]
Cited for: ACTIVE SITE LYS-631, MUTAGENESIS OF LYS-631.
[7]"Site-specific mutagenesis of residue Lys-172 of phage T7 RNA polymerase: characterization of transcription properties of mutant proteins."
Lyakhov D.L., Ilgenfrits H., Chernov B.K., Dragan S.M., Rechinsky V.O., Pokholok D.K., Tunitskaya V.L., Kochetkov S.N.
Mol. Biol. (Mosk.) 26:1022-1035(1992) [PubMed: 1470170] [Abstract]
Cited for: MUTAGENESIS OF LYS-172.
[8]"Random mutagenesis of the gene for bacteriophage T7 RNA polymerase."
Rechinsky V.O., Kostyuk D.A., Lyakhov D.L., Chernov B.K., Kochetkov S.N.
Mol. Gen. Genet. 238:455-458(1993) [PubMed: 8492813] [Abstract]
Cited for: MUTAGENESIS.
[9]"Tyr-571 is involved in the T7 RNA polymerase binding to its promoter."
Rechinsky V.O., Tunitskaya V.L., Dragan S.M., Kostyuk D.A., Kochetkov S.N.
FEBS Lett. 320:9-12(1993) [PubMed: 8462683] [Abstract]
Cited for: MUTAGENESIS.
[10]"Bacteriophage T7 RNA polymerase and its active-site mutants. Kinetic, spectroscopic and calorimetric characterization."
Osumi-Davis P., Sreerama N., Volkin D.B., Middaugh C.R., Woody R.W., Woody A.-Y.M.
J. Mol. Biol. 237:5-19(1994) [PubMed: 8133519] [Abstract]
Cited for: MUTAGENESIS.
[11]"Crystal structure of bacteriophage T7 RNA polymerase at 3.3-A resolution."
Sousa R., Chung Y.J., Rose J.P., Wang B.-C.
Nature 364:593-599(1993) [PubMed: 7688864] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).
[12]"Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme."
Jeruzalmi D., Steitz T.A.
EMBO J. 17:4101-4113(1998) [PubMed: 9670025] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF COMPLEX WITH LYSOZYME.
+Additional computationally mapped references.

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Cross-references

Sequence databases

V01146 Genomic DNA. Translation: CAA24390.1.
V01127 Genomic DNA. Translation: CAA24333.1.
M38308 Genomic DNA. Translation: AAA32569.1.
PIRRNBP17. A94615.
RefSeqNP_041960.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1AROX-ray2.80P1-883[»]
1CEZX-ray2.40A1-883[»]
1H38X-ray2.90A/B/C/D1-883[»]
1MSWX-ray2.10D1-883[»]
1QLNX-ray2.40A1-883[»]
1S0VX-ray3.20A/B/C/D1-882[»]
1S76X-ray2.88D1-883[»]
1S77X-ray2.69D1-883[»]
2PI4X-ray2.50A6-883[»]
2PI5X-ray2.90A6-883[»]
3E2EX-ray3.00A1-883[»]
3E3JX-ray6.70B/C1-883[»]
4RNPX-ray3.00A/B/C1-883[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6091N.
IntActP00573. 1 interaction.

Genome annotation databases

GeneID1261050.
GenomeReviewsGene locus 1 in contig V01146_GR.

Enzyme and pathway databases

BRENDA2.7.7.6. 1115.

Family and domain databases

InterProIPR002092. DNA-dir_RNA_pol_phage.
[Graphical view]
PANTHERPTHR10102. RNA_pol_phage. 1 hit.
PfamPF00940. RNA_pol. 1 hit.
[Graphical view]
PROSITEPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

PMAP-CutDBP00573.

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Entry information

Entry nameRPOL_BPT7
AccessionPrimary (citable) accession number: P00573
Secondary accession number(s): Q38543
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 11, 2001
Last modified: June 16, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents