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Protein

T7 RNA polymerase

Gene

1

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an "abortive phase" where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.5 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei5371 Publication1
Active sitei6312 Publications1
Active sitei8121 Publication1

GO - Molecular functioni

GO - Biological processi

  • DNA-templated viral transcription Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription, Viral transcription

Enzyme and pathway databases

BRENDAi2.7.7.6. 736.

Names & Taxonomyi

Protein namesi
Recommended name:
T7 RNA polymerase
Alternative name(s):
DNA-directed RNA polymerase (EC:2.7.7.61 Publication)
Gene namesi
Ordered Locus Names:1
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172K → L or G: No change in activity. 1 Publication1
Mutagenesisi563P → A or T: Inactivated. 1
Mutagenesisi571Y → S: Inactivated. 1
Mutagenesisi631K → G: Partially inactivated. 1 Publication1
Mutagenesisi631K → L: Partially inactivated. 1 Publication1
Mutagenesisi631K → R: Partially inactivated. 1 Publication1
Mutagenesisi636T → P: Inactivated. 1
Mutagenesisi639Y → D: Inactivated. 1
Mutagenesisi646F → C: Inactivated. 1

Chemistry databases

ChEMBLiCHEMBL1075072.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877491 – 883T7 RNA polymeraseAdd BLAST883

Proteomic databases

PRIDEiP00573.

Miscellaneous databases

PMAP-CutDBP00573.

Interactioni

Subunit structurei

Monomer. Interacts with T7 lysozyme; this interaction inhibits transcriptional function of T7 RNA polymerase.

Protein-protein interaction databases

DIPiDIP-6091N.
IntActiP00573. 1 interactor.
MINTiMINT-1513587.

Chemistry databases

BindingDBiP00573.

Structurei

Secondary structure

1883
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi12 – 14Combined sources3
Helixi18 – 24Combined sources7
Turni25 – 28Combined sources4
Helixi30 – 59Combined sources30
Turni60 – 62Combined sources3
Helixi64 – 66Combined sources3
Turni68 – 70Combined sources3
Helixi71 – 94Combined sources24
Turni101 – 107Combined sources7
Helixi111 – 128Combined sources18
Beta strandi129 – 131Combined sources3
Helixi134 – 156Combined sources23
Helixi162 – 165Combined sources4
Helixi168 – 172Combined sources5
Helixi176 – 189Combined sources14
Turni191 – 198Combined sources8
Turni205 – 207Combined sources3
Helixi208 – 223Combined sources16
Beta strandi227 – 230Combined sources4
Beta strandi231 – 233Combined sources3
Turni237 – 239Combined sources3
Beta strandi243 – 246Combined sources4
Helixi248 – 261Combined sources14
Beta strandi271 – 273Combined sources3
Beta strandi279 – 283Combined sources5
Beta strandi285 – 288Combined sources4
Beta strandi289 – 291Combined sources3
Beta strandi295 – 298Combined sources4
Helixi304 – 307Combined sources4
Turni308 – 310Combined sources3
Helixi314 – 324Combined sources11
Beta strandi328 – 330Combined sources3
Helixi332 – 341Combined sources10
Beta strandi344 – 346Combined sources3
Beta strandi348 – 351Combined sources4
Beta strandi365 – 368Combined sources4
Turni370 – 372Combined sources3
Helixi374 – 377Combined sources4
Helixi379 – 402Combined sources24
Turni403 – 405Combined sources3
Beta strandi406 – 410Combined sources5
Beta strandi418 – 421Combined sources4
Beta strandi422 – 424Combined sources3
Beta strandi426 – 432Combined sources7
Beta strandi434 – 436Combined sources3
Helixi438 – 443Combined sources6
Beta strandi444 – 448Combined sources5
Helixi453 – 467Combined sources15
Helixi475 – 484Combined sources10
Helixi486 – 494Combined sources9
Helixi496 – 499Combined sources4
Helixi501 – 504Combined sources4
Beta strandi505 – 507Combined sources3
Helixi508 – 524Combined sources17
Helixi525 – 527Combined sources3
Beta strandi528 – 530Combined sources3
Beta strandi534 – 537Combined sources4
Helixi541 – 550Combined sources10
Helixi555 – 558Combined sources4
Beta strandi559 – 564Combined sources6
Helixi570 – 586Combined sources17
Beta strandi594 – 596Combined sources3
Beta strandi597 – 602Combined sources6
Beta strandi605 – 610Combined sources6
Helixi613 – 621Combined sources9
Helixi627 – 635Combined sources9
Helixi636 – 639Combined sources4
Helixi643 – 653Combined sources11
Helixi655 – 660Combined sources6
Turni662 – 666Combined sources5
Helixi670 – 688Combined sources19
Helixi690 – 707Combined sources18
Turni713 – 715Combined sources3
Beta strandi718 – 720Combined sources3
Beta strandi725 – 728Combined sources4
Turni730 – 732Combined sources3
Beta strandi734 – 737Combined sources4
Beta strandi740 – 746Combined sources7
Beta strandi747 – 749Combined sources3
Beta strandi750 – 752Combined sources3
Beta strandi755 – 757Combined sources3
Beta strandi760 – 769Combined sources10
Helixi771 – 801Combined sources31
Beta strandi807 – 809Combined sources3
Beta strandi811 – 816Combined sources6
Helixi818 – 820Combined sources3
Helixi821 – 838Combined sources18
Helixi841 – 849Combined sources9
Turni852 – 854Combined sources3
Turni855 – 858Combined sources4
Beta strandi859 – 861Combined sources3
Helixi872 – 876Combined sources5
Turni879 – 881Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AROX-ray2.80P1-883[»]
1CEZX-ray2.40A1-883[»]
1H38X-ray2.90A/B/C/D1-883[»]
1MSWX-ray2.10D1-883[»]
1QLNX-ray2.40A1-883[»]
1S0VX-ray3.20A/B/C/D1-883[»]
1S76X-ray2.88D1-883[»]
1S77X-ray2.69D1-883[»]
2PI4X-ray2.50A6-883[»]
2PI5X-ray2.90A6-883[»]
3E2EX-ray3.00A1-883[»]
3E3JX-ray6.70B/C1-883[»]
4RNPX-ray3.00A/B/C1-883[»]
ProteinModelPortaliP00573.
SMRiP00573.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00573.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi380 – 383Poly-Ala4

Sequence similaritiesi

Phylogenomic databases

KOiK10908.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 1 hit.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTINIAKND FSDIELAAIP FNTLADHYGE RLAREQLALE HESYEMGEAR
60 70 80 90 100
FRKMFERQLK AGEVADNAAA KPLITTLLPK MIARINDWFE EVKAKRGKRP
110 120 130 140 150
TAFQFLQEIK PEAVAYITIK TTLACLTSAD NTTVQAVASA IGRAIEDEAR
160 170 180 190 200
FGRIRDLEAK HFKKNVEEQL NKRVGHVYKK AFMQVVEADM LSKGLLGGEA
210 220 230 240 250
WSSWHKEDSI HVGVRCIEML IESTGMVSLH RQNAGVVGQD SETIELAPEY
260 270 280 290 300
AEAIATRAGA LAGISPMFQP CVVPPKPWTG ITGGGYWANG RRPLALVRTH
310 320 330 340 350
SKKALMRYED VYMPEVYKAI NIAQNTAWKI NKKVLAVANV ITKWKHCPVE
360 370 380 390 400
DIPAIEREEL PMKPEDIDMN PEALTAWKRA AAAVYRKDKA RKSRRISLEF
410 420 430 440 450
MLEQANKFAN HKAIWFPYNM DWRGRVYAVS MFNPQGNDMT KGLLTLAKGK
460 470 480 490 500
PIGKEGYYWL KIHGANCAGV DKVPFPERIK FIEENHENIM ACAKSPLENT
510 520 530 540 550
WWAEQDSPFC FLAFCFEYAG VQHHGLSYNC SLPLAFDGSC SGIQHFSAML
560 570 580 590 600
RDEVGGRAVN LLPSETVQDI YGIVAKKVNE ILQADAINGT DNEVVTVTDE
610 620 630 640 650
NTGEISEKVK LGTKALAGQW LAYGVTRSVT KRSVMTLAYG SKEFGFRQQV
660 670 680 690 700
LEDTIQPAID SGKGLMFTQP NQAAGYMAKL IWESVSVTVV AAVEAMNWLK
710 720 730 740 750
SAAKLLAAEV KDKKTGEILR KRCAVHWVTP DGFPVWQEYK KPIQTRLNLM
760 770 780 790 800
FLGQFRLQPT INTNKDSEID AHKQESGIAP NFVHSQDGSH LRKTVVWAHE
810 820 830 840 850
KYGIESFALI HDSFGTIPAD AANLFKAVRE TMVDTYESCD VLADFYDQFA
860 870 880
DQLHESQLDK MPALPAKGNL NLRDILESDF AFA
Length:883
Mass (Da):98,855
Last modified:January 11, 2001 - v2
Checksum:i7FBDEAC457842549
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388 – 424DKARK…MDWRG → TRLASLAVSALSSCLSKPIS LLTIRPSGSLTTWTGAV in CAA24333 (PubMed:7310873).CuratedAdd BLAST37
Sequence conflicti389K → R in AAA32569 (PubMed:6093820).Curated1
Sequence conflicti443L → R in CAA24333 (PubMed:7310873).Curated1
Sequence conflicti474P → S in CAA24333 (PubMed:7310873).Curated1
Sequence conflicti623Y → H in AAA32569 (PubMed:6093820).Curated1
Sequence conflicti665L → P in AAA32569 (PubMed:6093820).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24390.1.
V01127 Genomic DNA. Translation: CAA24333.1.
M38308 Genomic DNA. Translation: AAA32569.1.
PIRiA94615. RNBP17.
RefSeqiNP_041960.1. NC_001604.1.

Genome annotation databases

GeneIDi1261050.
KEGGivg:1261050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24390.1.
V01127 Genomic DNA. Translation: CAA24333.1.
M38308 Genomic DNA. Translation: AAA32569.1.
PIRiA94615. RNBP17.
RefSeqiNP_041960.1. NC_001604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AROX-ray2.80P1-883[»]
1CEZX-ray2.40A1-883[»]
1H38X-ray2.90A/B/C/D1-883[»]
1MSWX-ray2.10D1-883[»]
1QLNX-ray2.40A1-883[»]
1S0VX-ray3.20A/B/C/D1-883[»]
1S76X-ray2.88D1-883[»]
1S77X-ray2.69D1-883[»]
2PI4X-ray2.50A6-883[»]
2PI5X-ray2.90A6-883[»]
3E2EX-ray3.00A1-883[»]
3E3JX-ray6.70B/C1-883[»]
4RNPX-ray3.00A/B/C1-883[»]
ProteinModelPortaliP00573.
SMRiP00573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6091N.
IntActiP00573. 1 interactor.
MINTiMINT-1513587.

Chemistry databases

BindingDBiP00573.
ChEMBLiCHEMBL1075072.

Proteomic databases

PRIDEiP00573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261050.
KEGGivg:1261050.

Phylogenomic databases

KOiK10908.

Enzyme and pathway databases

BRENDAi2.7.7.6. 736.

Miscellaneous databases

EvolutionaryTraceiP00573.
PMAP-CutDBP00573.
PROiP00573.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 1 hit.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOL_BPT7
AccessioniPrimary (citable) accession number: P00573
Secondary accession number(s): Q38543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 11, 2001
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.