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P00572 (KTHY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate kinase

EC=2.7.4.9
Alternative name(s):
dTMP kinase
Gene names
Name:CDC8
Ordered Locus Names:YJR057W
ORF Names:J1715
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of dTMP to dTDP. HAMAP-Rule MF_00165

Catalytic activity

ATP + dTMP = ADP + dTDP. HAMAP-Rule MF_00165

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00165

Subunit structure

Homodimer.

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the thymidylate kinase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-9957,EBI-9957
NET1P470352EBI-9957,EBI-25953

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Thymidylate kinase HAMAP-Rule MF_00165
PRO_0000155214

Regions

Nucleotide binding12 – 198ATP Probable

Experimental info

Sequence conflict571S → T in AAA34486. Ref.2
Sequence conflict1881G → D in AAA35158. Ref.1

Secondary structure

................................... 216
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00572 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: F9A1CA0D56DCD1F6

FASTA21624,687
        10         20         30         40         50         60 
MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN EYLTDDSFQL 

        70         80         90        100        110        120 
SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV AYSAAKGTNG MDLDWCLQPD 

       130        140        150        160        170        180 
VGLLKPDLTL FLSTQDVDNN AEKSGFGDER YETVKFQEKV KQTFMKLLDK EIRKGDESIT 

       190        200        210 
IVDVTNKGIQ EVEALIWQIV EPVLSTHIDH DKFSFF 

« Hide

References

« Hide 'large scale' references
[1]"The CDC8 gene of yeast encodes thymidylate kinase."
Jong A.Y.S., Kuo C.-L., Campbell J.L.
J. Biol. Chem. 259:11052-11059(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Saccharomyces cerevisiae CDC8 gene and its product."
Birkenmeyer L.G., Hill J.C., Dumas L.B.
Mol. Cell. Biol. 4:583-590(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation."
Lavie A., Vetter I.R., Konrad M., Goody R.S., Reinstein J., Schlichting I.
Nat. Struct. Biol. 4:601-604(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[8]"Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0-A resolution: implications for catalysis and AZT activation."
Lavie A., Konrad M., Brundiers R., Goody R.S., Schlichting I., Reinstein J.
Biochemistry 37:3677-3686(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02116 Genomic DNA. Translation: AAA35158.1.
K01783 Genomic DNA. Translation: AAA34486.1.
Z49557 Genomic DNA. Translation: CAA89585.1.
M15468 Genomic DNA. Translation: AAB05644.1.
L47993 Genomic DNA. Translation: AAB39283.1.
BK006943 Genomic DNA. Translation: DAA08844.1.
PIRKIBYT8. S57076.
RefSeqNP_012591.1. NM_001181715.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TMKX-ray2.10A/B1-216[»]
2TMKX-ray2.40A/B1-216[»]
3TMKX-ray2.00A/B/C/D/E/F/G/H1-216[»]
ProteinModelPortalP00572.
SMRP00572. Positions 1-216.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33814. 87 interactions.
IntActP00572. 5 interactions.
MINTMINT-662334.
STRING4932.YJR057W.

Proteomic databases

PaxDbP00572.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR057W; YJR057W; YJR057W.
GeneID853520.
KEGGsce:YJR057W.

Organism-specific databases

CYGDYJR057w.
SGDS000003818. CDC8.

Phylogenomic databases

eggNOGCOG0125.
GeneTreeENSGT00390000017546.
HOGENOMHOG000229079.
KOK00943.
OMAGKMIDSY.
OrthoDBEOG735483.

Enzyme and pathway databases

BioCycMetaCyc:YJR057W-MONOMER.
YEAST:YJR057W-MONOMER.
UniPathwayUPA00575.

Gene expression databases

GenevestigatorP00572.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00165. Thymidylate_kinase.
InterProIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00041. DTMP_kinase. 1 hit.
PROSITEPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00572.
NextBio974197.
PROP00572.

Entry information

Entry nameKTHY_YEAST
AccessionPrimary (citable) accession number: P00572
Secondary accession number(s): D6VWM8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways