SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00572

- KTHY_YEAST

UniProt

P00572 - KTHY_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Thymidylate kinase
Gene
CDC8, YJR057W, J1715
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dTMP to dTDP.UniRule annotation

Catalytic activityi

ATP + dTMP = ADP + dTDP.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198ATP Inferred

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. identical protein binding Source: IntAct
  3. protein binding Source: IntAct
  4. thymidylate kinase activity Source: SGD
  5. uridylate kinase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. dTDP biosynthetic process Source: SGD
  2. dTTP biosynthetic process Source: SGD
  3. dUDP biosynthetic process Source: SGD
  4. nucleotide phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YJR057W-MONOMER.
YEAST:YJR057W-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
dTMP kinase
Gene namesi
Name:CDC8
Ordered Locus Names:YJR057W
ORF Names:J1715
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR057w.
SGDiS000003818. CDC8.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Thymidylate kinaseUniRule annotation
PRO_0000155214Add
BLAST

Proteomic databases

MaxQBiP00572.
PaxDbiP00572.

Expressioni

Gene expression databases

GenevestigatoriP00572.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9957,EBI-9957
NET1P470352EBI-9957,EBI-25953

Protein-protein interaction databases

BioGridi33814. 87 interactions.
IntActiP00572. 5 interactions.
MINTiMINT-662334.
STRINGi4932.YJR057W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126
Beta strandi14 – 174
Helixi18 – 2912
Beta strandi31 – 399
Helixi44 – 5411
Helixi62 – 7413
Helixi77 – 859
Beta strandi89 – 946
Helixi96 – 1049
Helixi113 – 1175
Helixi118 – 1203
Beta strandi123 – 1253
Beta strandi127 – 1337
Helixi140 – 1423
Beta strandi145 – 1473
Helixi154 – 17320
Beta strandi178 – 1836
Helixi189 – 20517

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TMKX-ray2.10A/B1-216[»]
2TMKX-ray2.40A/B1-216[»]
3TMKX-ray2.00A/B/C/D/E/F/G/H1-216[»]
ProteinModelPortaliP00572.
SMRiP00572. Positions 1-216.

Miscellaneous databases

EvolutionaryTraceiP00572.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0125.
GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
KOiK00943.
OMAiGKMIDSY.
OrthoDBiEOG735483.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00572-1 [UniParc]FASTAAdd to Basket

« Hide

MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN    50
EYLTDDSFQL SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV 100
AYSAAKGTNG MDLDWCLQPD VGLLKPDLTL FLSTQDVDNN AEKSGFGDER 150
YETVKFQEKV KQTFMKLLDK EIRKGDESIT IVDVTNKGIQ EVEALIWQIV 200
EPVLSTHIDH DKFSFF 216
Length:216
Mass (Da):24,687
Last modified:November 1, 1995 - v2
Checksum:iF9A1CA0D56DCD1F6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571S → T in AAA34486. 1 Publication
Sequence conflicti188 – 1881G → D in AAA35158. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02116 Genomic DNA. Translation: AAA35158.1.
K01783 Genomic DNA. Translation: AAA34486.1.
Z49557 Genomic DNA. Translation: CAA89585.1.
M15468 Genomic DNA. Translation: AAB05644.1.
L47993 Genomic DNA. Translation: AAB39283.1.
BK006943 Genomic DNA. Translation: DAA08844.1.
PIRiS57076. KIBYT8.
RefSeqiNP_012591.1. NM_001181715.1.

Genome annotation databases

EnsemblFungiiYJR057W; YJR057W; YJR057W.
GeneIDi853520.
KEGGisce:YJR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02116 Genomic DNA. Translation: AAA35158.1 .
K01783 Genomic DNA. Translation: AAA34486.1 .
Z49557 Genomic DNA. Translation: CAA89585.1 .
M15468 Genomic DNA. Translation: AAB05644.1 .
L47993 Genomic DNA. Translation: AAB39283.1 .
BK006943 Genomic DNA. Translation: DAA08844.1 .
PIRi S57076. KIBYT8.
RefSeqi NP_012591.1. NM_001181715.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TMK X-ray 2.10 A/B 1-216 [» ]
2TMK X-ray 2.40 A/B 1-216 [» ]
3TMK X-ray 2.00 A/B/C/D/E/F/G/H 1-216 [» ]
ProteinModelPortali P00572.
SMRi P00572. Positions 1-216.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33814. 87 interactions.
IntActi P00572. 5 interactions.
MINTi MINT-662334.
STRINGi 4932.YJR057W.

Proteomic databases

MaxQBi P00572.
PaxDbi P00572.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR057W ; YJR057W ; YJR057W .
GeneIDi 853520.
KEGGi sce:YJR057W.

Organism-specific databases

CYGDi YJR057w.
SGDi S000003818. CDC8.

Phylogenomic databases

eggNOGi COG0125.
GeneTreei ENSGT00390000017546.
HOGENOMi HOG000229079.
KOi K00943.
OMAi GKMIDSY.
OrthoDBi EOG735483.

Enzyme and pathway databases

UniPathwayi UPA00575 .
BioCyci MetaCyc:YJR057W-MONOMER.
YEAST:YJR057W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P00572.
NextBioi 974197.
PROi P00572.

Gene expression databases

Genevestigatori P00572.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00165. Thymidylate_kinase.
InterProi IPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00041. DTMP_kinase. 1 hit.
PROSITEi PS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CDC8 gene of yeast encodes thymidylate kinase."
    Jong A.Y.S., Kuo C.-L., Campbell J.L.
    J. Biol. Chem. 259:11052-11059(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Saccharomyces cerevisiae CDC8 gene and its product."
    Birkenmeyer L.G., Hill J.C., Dumas L.B.
    Mol. Cell. Biol. 4:583-590(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation."
    Lavie A., Vetter I.R., Konrad M., Goody R.S., Reinstein J., Schlichting I.
    Nat. Struct. Biol. 4:601-604(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  8. "Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0-A resolution: implications for catalysis and AZT activation."
    Lavie A., Konrad M., Brundiers R., Goody R.S., Schlichting I., Reinstein J.
    Biochemistry 37:3677-3686(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiKTHY_YEAST
AccessioniPrimary (citable) accession number: P00572
Secondary accession number(s): D6VWM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi