P00572 (KTHY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate kinase
EC=2.7.4.9

Alternative name(s):
dTMP kinase

Gene names

Name:	CDC8
Ordered Locus Names:	YJR057W
ORF Names:	J1715

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of dTMP to dTDP.
Catalytic activity	ATP + dTMP = ADP + dTDP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer.
Miscellaneous	Present with 2980 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the thymidylate kinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTDP biosynthetic process Inferred from direct assay PubMed 6094555. Source: SGD dTTP biosynthetic process Inferred from direct assay PubMed 6094555. Source: SGD dUDP biosynthetic process Inferred from direct assay PubMed 6094555. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 6094555. Source: SGD nucleus Inferred from direct assay PubMed 6094555. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidylate kinase activity Inferred from direct assay Ref.1PubMed 6094555. Source: SGD uridylate kinase activity Inferred from direct assay PubMed 6094555. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-9957,EBI-9957
NET1	P47035	2	EBI-9957,EBI-25953

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 216

216

Thymidylate kinase

PRO_0000155214

Regions

Nucleotide binding

12 – 19

8

ATP Probable

Experimental info

Sequence conflict

57

1

S → T in AAA34486. Ref.2

Sequence conflict

188

1

G → D in AAA35158. Ref.1

Secondary structure

1

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216

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Beta strand

14 – 17

4

Helix

18 – 29

12

Beta strand

31 – 39

9

Helix

44 – 54

11

Helix

62 – 74

13

Helix

77 – 85

9

Beta strand

89 – 94

6

Helix

96 – 104

9

Helix

113 – 117

5

Helix

118 – 120

3

Beta strand

123 – 125

3

Beta strand

127 – 133

7

Helix

140 – 142

3

Beta strand

145 – 147

3

Helix

154 – 173

20

Beta strand

178 – 183

6

Helix

189 – 205

17

Sequences

	Sequence		Length	Mass (Da)
	P00572 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: F9A1CA0D56DCD1F6	FASTA	216	24,687
	10 20 30 40 50 60 MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN EYLTDDSFQL 70 80 90 100 110 120 SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV AYSAAKGTNG MDLDWCLQPD 130 140 150 160 170 180 VGLLKPDLTL FLSTQDVDNN AEKSGFGDER YETVKFQEKV KQTFMKLLDK EIRKGDESIT 190 200 210 IVDVTNKGIQ EVEALIWQIV EPVLSTHIDH DKFSFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CDC8 gene of yeast encodes thymidylate kinase." Jong A.Y.S., Kuo C.-L., Campbell J.L. J. Biol. Chem. 259:11052-11059(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Saccharomyces cerevisiae CDC8 gene and its product." Birkenmeyer L.G., Hill J.C., Dumas L.B. Mol. Cell. Biol. 4:583-590(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation." Lavie A., Vetter I.R., Konrad M., Goody R.S., Reinstein J., Schlichting I. Nat. Struct. Biol. 4:601-604(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[8]	"Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0-A resolution: implications for catalysis and AZT activation." Lavie A., Konrad M., Brundiers R., Goody R.S., Schlichting I., Reinstein J. Biochemistry 37:3677-3686(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02116 Genomic DNA. Translation: AAA35158.1.
K01783 Genomic DNA. Translation: AAA34486.1.
Z49557 Genomic DNA. Translation: CAA89585.1.
M15468 Genomic DNA. Translation: AAB05644.1.
L47993 Genomic DNA. Translation: AAB39283.1.
BK006943 Genomic DNA. Translation: DAA08844.1.

PIR

KIBYT8. S57076.

RefSeq

NP_012591.1. NM_001181715.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TMK	X-ray	2.10	A/B	1-216	[»]
2TMK	X-ray	2.40	A/B	1-216	[»]
3TMK	X-ray	2.00	A/B/C/D/E/F/G/H	1-216	[»]

ProteinModelPortal

P00572.

SMR

P00572. Positions 1-216.

ModBase

Search...

Protein-protein interaction databases

IntAct

P00572. 5 interactions.

MINT

MINT-662334.

STRING

4932.YJR057W.

Proteomic databases

PaxDb

P00572.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJR057W; YJR057W; YJR057W.

GeneID

853520.

KEGG

sce:YJR057W.

Organism-specific databases

CYGD

YJR057w.

SGD

S000003818. CDC8.

Phylogenomic databases

eggNOG

COG0125.

GeneTree

ENSGT00390000017546.

HOGENOM

HOG000229079.

KO

K00943.

OMA

YSGVAYS.

OrthoDB

EOG4M68T4.

Enzyme and pathway databases

UniPathway

UPA00575.

Gene expression databases

Genevestigator

P00572.

GermOnline

YJR057W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]

PANTHER

PTHR10344. PTHR10344. 1 hit.

TIGRFAMs

TIGR00041. DTMP_kinase. 1 hit.

PROSITE

PS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P00572.

NextBio

974197.

Entry information

Entry name

KTHY_YEAST

Accession

Primary (citable) accession number: P00572
Secondary accession number(s): D6VWM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program