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P00572

- KTHY_YEAST

UniProt

P00572 - KTHY_YEAST

Protein

Thymidylate kinase

Gene

CDC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dTMP to dTDP.

    Catalytic activityi

    ATP + dTMP = ADP + dTDP.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 198ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. protein binding Source: IntAct
    4. thymidylate kinase activity Source: SGD
    5. uridylate kinase activity Source: SGD

    GO - Biological processi

    1. dTDP biosynthetic process Source: SGD
    2. dTTP biosynthetic process Source: SGD
    3. dUDP biosynthetic process Source: SGD
    4. nucleotide phosphorylation Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YJR057W-MONOMER.
    YEAST:YJR057W-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate kinase (EC:2.7.4.9)
    Alternative name(s):
    dTMP kinase
    Gene namesi
    Name:CDC8
    Ordered Locus Names:YJR057W
    ORF Names:J1715
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR057w.
    SGDiS000003818. CDC8.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Thymidylate kinasePRO_0000155214Add
    BLAST

    Proteomic databases

    MaxQBiP00572.
    PaxDbiP00572.

    Expressioni

    Gene expression databases

    GenevestigatoriP00572.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-9957,EBI-9957
    NET1P470352EBI-9957,EBI-25953

    Protein-protein interaction databases

    BioGridi33814. 87 interactions.
    IntActiP00572. 5 interactions.
    MINTiMINT-662334.
    STRINGi4932.YJR057W.

    Structurei

    Secondary structure

    1
    216
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 126
    Beta strandi14 – 174
    Helixi18 – 2912
    Beta strandi31 – 399
    Helixi44 – 5411
    Helixi62 – 7413
    Helixi77 – 859
    Beta strandi89 – 946
    Helixi96 – 1049
    Helixi113 – 1175
    Helixi118 – 1203
    Beta strandi123 – 1253
    Beta strandi127 – 1337
    Helixi140 – 1423
    Beta strandi145 – 1473
    Helixi154 – 17320
    Beta strandi178 – 1836
    Helixi189 – 20517

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TMKX-ray2.10A/B1-216[»]
    2TMKX-ray2.40A/B1-216[»]
    3TMKX-ray2.00A/B/C/D/E/F/G/H1-216[»]
    ProteinModelPortaliP00572.
    SMRiP00572. Positions 1-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00572.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0125.
    GeneTreeiENSGT00390000017546.
    HOGENOMiHOG000229079.
    KOiK00943.
    OMAiGKMIDSY.
    OrthoDBiEOG735483.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00165. Thymidylate_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR018095. Thymidylate_kin_CS.
    IPR018094. Thymidylate_kinase.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
    PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00572-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN    50
    EYLTDDSFQL SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV 100
    AYSAAKGTNG MDLDWCLQPD VGLLKPDLTL FLSTQDVDNN AEKSGFGDER 150
    YETVKFQEKV KQTFMKLLDK EIRKGDESIT IVDVTNKGIQ EVEALIWQIV 200
    EPVLSTHIDH DKFSFF 216
    Length:216
    Mass (Da):24,687
    Last modified:November 1, 1995 - v2
    Checksum:iF9A1CA0D56DCD1F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571S → T in AAA34486. (PubMed:6371491)Curated
    Sequence conflicti188 – 1881G → D in AAA35158. (PubMed:6088527)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02116 Genomic DNA. Translation: AAA35158.1.
    K01783 Genomic DNA. Translation: AAA34486.1.
    Z49557 Genomic DNA. Translation: CAA89585.1.
    M15468 Genomic DNA. Translation: AAB05644.1.
    L47993 Genomic DNA. Translation: AAB39283.1.
    BK006943 Genomic DNA. Translation: DAA08844.1.
    PIRiS57076. KIBYT8.
    RefSeqiNP_012591.1. NM_001181715.1.

    Genome annotation databases

    EnsemblFungiiYJR057W; YJR057W; YJR057W.
    GeneIDi853520.
    KEGGisce:YJR057W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02116 Genomic DNA. Translation: AAA35158.1 .
    K01783 Genomic DNA. Translation: AAA34486.1 .
    Z49557 Genomic DNA. Translation: CAA89585.1 .
    M15468 Genomic DNA. Translation: AAB05644.1 .
    L47993 Genomic DNA. Translation: AAB39283.1 .
    BK006943 Genomic DNA. Translation: DAA08844.1 .
    PIRi S57076. KIBYT8.
    RefSeqi NP_012591.1. NM_001181715.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TMK X-ray 2.10 A/B 1-216 [» ]
    2TMK X-ray 2.40 A/B 1-216 [» ]
    3TMK X-ray 2.00 A/B/C/D/E/F/G/H 1-216 [» ]
    ProteinModelPortali P00572.
    SMRi P00572. Positions 1-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33814. 87 interactions.
    IntActi P00572. 5 interactions.
    MINTi MINT-662334.
    STRINGi 4932.YJR057W.

    Proteomic databases

    MaxQBi P00572.
    PaxDbi P00572.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR057W ; YJR057W ; YJR057W .
    GeneIDi 853520.
    KEGGi sce:YJR057W.

    Organism-specific databases

    CYGDi YJR057w.
    SGDi S000003818. CDC8.

    Phylogenomic databases

    eggNOGi COG0125.
    GeneTreei ENSGT00390000017546.
    HOGENOMi HOG000229079.
    KOi K00943.
    OMAi GKMIDSY.
    OrthoDBi EOG735483.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci MetaCyc:YJR057W-MONOMER.
    YEAST:YJR057W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P00572.
    NextBioi 974197.
    PROi P00572.

    Gene expression databases

    Genevestigatori P00572.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00165. Thymidylate_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR018095. Thymidylate_kin_CS.
    IPR018094. Thymidylate_kinase.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00041. DTMP_kinase. 1 hit.
    PROSITEi PS01331. THYMIDYLATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CDC8 gene of yeast encodes thymidylate kinase."
      Jong A.Y.S., Kuo C.-L., Campbell J.L.
      J. Biol. Chem. 259:11052-11059(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Saccharomyces cerevisiae CDC8 gene and its product."
      Birkenmeyer L.G., Hill J.C., Dumas L.B.
      Mol. Cell. Biol. 4:583-590(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation."
      Lavie A., Vetter I.R., Konrad M., Goody R.S., Reinstein J., Schlichting I.
      Nat. Struct. Biol. 4:601-604(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    8. "Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0-A resolution: implications for catalysis and AZT activation."
      Lavie A., Konrad M., Brundiers R., Goody R.S., Schlichting I., Reinstein J.
      Biochemistry 37:3677-3686(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiKTHY_YEAST
    AccessioniPrimary (citable) accession number: P00572
    Secondary accession number(s): D6VWM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2980 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3