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Protein

Thymidylate kinase

Gene

CDC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dTMP to dTDP.

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • thymidylate kinase activity Source: SGD
  • uridylate kinase activity Source: SGD

GO - Biological processi

  • dTDP biosynthetic process Source: SGD
  • dTTP biosynthetic process Source: SGD
  • dUDP biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YJR057W-MONOMER.
YEAST:YJR057W-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
dTMP kinase
Gene namesi
Name:CDC8
Ordered Locus Names:YJR057W
ORF Names:J1715
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR057W.
SGDiS000003818. CDC8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: GO_Central
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001552141 – 216Thymidylate kinaseAdd BLAST216

Proteomic databases

MaxQBiP00572.
PRIDEiP00572.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9957,EBI-9957
NET1P470352EBI-9957,EBI-25953

Protein-protein interaction databases

BioGridi33814. 88 interactors.
IntActiP00572. 5 interactors.
MINTiMINT-662334.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Beta strandi14 – 17Combined sources4
Helixi18 – 29Combined sources12
Beta strandi31 – 39Combined sources9
Helixi44 – 54Combined sources11
Helixi62 – 74Combined sources13
Helixi77 – 85Combined sources9
Beta strandi89 – 94Combined sources6
Helixi96 – 104Combined sources9
Helixi113 – 117Combined sources5
Helixi118 – 120Combined sources3
Beta strandi123 – 125Combined sources3
Beta strandi127 – 133Combined sources7
Helixi140 – 142Combined sources3
Beta strandi145 – 147Combined sources3
Helixi154 – 173Combined sources20
Beta strandi178 – 183Combined sources6
Helixi189 – 205Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TMKX-ray2.10A/B1-216[»]
2TMKX-ray2.40A/B1-216[»]
3TMKX-ray2.00A/B/C/D/E/F/G/H1-216[»]
ProteinModelPortaliP00572.
SMRiP00572.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00572.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
InParanoidiP00572.
KOiK00943.
OMAiWAWQPEI.
OrthoDBiEOG092C4VOX.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN
60 70 80 90 100
EYLTDDSFQL SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV
110 120 130 140 150
AYSAAKGTNG MDLDWCLQPD VGLLKPDLTL FLSTQDVDNN AEKSGFGDER
160 170 180 190 200
YETVKFQEKV KQTFMKLLDK EIRKGDESIT IVDVTNKGIQ EVEALIWQIV
210
EPVLSTHIDH DKFSFF
Length:216
Mass (Da):24,687
Last modified:November 1, 1995 - v2
Checksum:iF9A1CA0D56DCD1F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57S → T in AAA34486 (PubMed:6371491).Curated1
Sequence conflicti188G → D in AAA35158 (PubMed:6088527).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02116 Genomic DNA. Translation: AAA35158.1.
K01783 Genomic DNA. Translation: AAA34486.1.
Z49557 Genomic DNA. Translation: CAA89585.1.
M15468 Genomic DNA. Translation: AAB05644.1.
L47993 Genomic DNA. Translation: AAB39283.1.
BK006943 Genomic DNA. Translation: DAA08844.1.
PIRiS57076. KIBYT8.
RefSeqiNP_012591.1. NM_001181715.1.

Genome annotation databases

EnsemblFungiiYJR057W; YJR057W; YJR057W.
GeneIDi853520.
KEGGisce:YJR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02116 Genomic DNA. Translation: AAA35158.1.
K01783 Genomic DNA. Translation: AAA34486.1.
Z49557 Genomic DNA. Translation: CAA89585.1.
M15468 Genomic DNA. Translation: AAB05644.1.
L47993 Genomic DNA. Translation: AAB39283.1.
BK006943 Genomic DNA. Translation: DAA08844.1.
PIRiS57076. KIBYT8.
RefSeqiNP_012591.1. NM_001181715.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TMKX-ray2.10A/B1-216[»]
2TMKX-ray2.40A/B1-216[»]
3TMKX-ray2.00A/B/C/D/E/F/G/H1-216[»]
ProteinModelPortaliP00572.
SMRiP00572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33814. 88 interactors.
IntActiP00572. 5 interactors.
MINTiMINT-662334.

Proteomic databases

MaxQBiP00572.
PRIDEiP00572.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR057W; YJR057W; YJR057W.
GeneIDi853520.
KEGGisce:YJR057W.

Organism-specific databases

EuPathDBiFungiDB:YJR057W.
SGDiS000003818. CDC8.

Phylogenomic databases

GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
InParanoidiP00572.
KOiK00943.
OMAiWAWQPEI.
OrthoDBiEOG092C4VOX.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciMetaCyc:YJR057W-MONOMER.
YEAST:YJR057W-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

EvolutionaryTraceiP00572.
PROiP00572.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKTHY_YEAST
AccessioniPrimary (citable) accession number: P00572
Secondary accession number(s): D6VWM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2980 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.