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Protein

Adenylate kinase isoenzyme 1

Gene

AK1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391AMPUniRule annotation
Binding sitei44 – 441AMPUniRule annotation
Binding sitei101 – 1011AMPUniRule annotation
Binding sitei132 – 1321ATPUniRule annotation
Binding sitei138 – 1381AMPUniRule annotation
Binding sitei149 – 1491AMPUniRule annotation
Binding sitei177 – 1771ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 236ATPUniRule annotation1 Publication
Nucleotide bindingi65 – 673AMPUniRule annotation
Nucleotide bindingi94 – 974AMPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SSC-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 1UniRule annotation (EC:2.7.4.3UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 1UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 1UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
MyokinaseUniRule annotation
Gene namesi
Name:AK1UniRule annotation
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Adenylate kinase isoenzyme 1PRO_0000158912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotation1 Publication
Modified residuei38 – 381PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00571.

Expressioni

Gene expression databases

GenevisibleiP00571. SS.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006028.

Structurei

Secondary structure

1
194
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 65Combined sources
Beta strandi10 – 156Combined sources
Helixi21 – 3111Combined sources
Beta strandi35 – 384Combined sources
Helixi39 – 4911Combined sources
Helixi52 – 6110Combined sources
Turni62 – 643Combined sources
Helixi69 – 8113Combined sources
Turni82 – 865Combined sources
Beta strandi90 – 945Combined sources
Helixi99 – 10810Combined sources
Beta strandi113 – 1197Combined sources
Helixi122 – 13615Combined sources
Helixi146 – 15611Combined sources
Helixi158 – 1647Combined sources
Turni165 – 1684Combined sources
Beta strandi170 – 1745Combined sources
Helixi179 – 19113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ADKX-ray2.10A1-194[»]
ProteinModelPortaliP00571.
SMRiP00571. Positions 1-194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00571.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 6730NMPbindUniRule annotation2 PublicationsAdd
BLAST
Regioni131 – 14111LIDUniRule annotation2 PublicationsAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation2 Publications

Sequence similaritiesi

Belongs to the adenylate kinase family. AK1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000238771.
HOVERGENiHBG108060.
InParanoidiP00571.
KOiK00939.
OMAiDCVLKKK.
OrthoDBiEOG7060S3.
TreeFamiTF354283.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03171. Adenylate_kinase_AK1.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR028582. AK1.
IPR006267. AK1/5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKLKKSKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG
60 70 80 90 100
SARGKMLSEI MEKGQLVPLE TVLDMLRDAM VAKVDTSKGF LIDGYPREVK
110 120 130 140 150
QGEEFERKIG QPTLLLYVDA GPETMTKRLL KRGETSGRVD DNEETIKKRL
160 170 180 190
ETYYKATEPV IAFYEKRGIV RKVNAEGSVD DVFSQVCTHL DTLK
Length:194
Mass (Da):21,639
Last modified:July 21, 1986 - v1
Checksum:i554202B49B1E74B4
GO

Sequence databases

PIRiA00682. KIPGA.
RefSeqiXP_003122225.3. XM_003122177.5.
XP_005660509.1. XM_005660452.2.
UniGeneiSsc.17611.

Genome annotation databases

EnsembliENSSSCT00000006187; ENSSSCP00000006028; ENSSSCG00000005627.
GeneIDi100521423.
KEGGissc:100521423.

Cross-referencesi

Sequence databases

PIRiA00682. KIPGA.
RefSeqiXP_003122225.3. XM_003122177.5.
XP_005660509.1. XM_005660452.2.
UniGeneiSsc.17611.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ADKX-ray2.10A1-194[»]
ProteinModelPortaliP00571.
SMRiP00571. Positions 1-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006028.

Proteomic databases

PaxDbiP00571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000006187; ENSSSCP00000006028; ENSSSCG00000005627.
GeneIDi100521423.
KEGGissc:100521423.

Organism-specific databases

CTDi203.

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000238771.
HOVERGENiHBG108060.
InParanoidiP00571.
KOiK00939.
OMAiDCVLKKK.
OrthoDBiEOG7060S3.
TreeFamiTF354283.

Enzyme and pathway databases

ReactomeiR-SSC-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

EvolutionaryTraceiP00571.

Gene expression databases

GenevisibleiP00571. SS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03171. Adenylate_kinase_AK1.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR028582. AK1.
IPR006267. AK1/5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino-acid sequence of sarcine adenylate kinase from skeletal muscle."
    Heil A., Mueller G., Noda L., Pinder T., Schirmer R.H., Schirmer I., von Zabern I.
    Eur. J. Biochem. 43:131-144(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Skeletal muscle.
  2. "ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins."
    Fry D.C., Kuby S.A., Mildvan A.S.
    Proc. Natl. Acad. Sci. U.S.A. 83:907-911(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: ATP-BINDING SITE.
  3. "Three dimensional structure of adenyl kinase."
    Schulz G.E., Elzinga M., Marx F., Schirmer R.H.
    Nature 250:120-123(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
  4. "Refined structure of porcine cytosolic adenylate kinase at 2.1 A resolution."
    Dreusicke D., Karplus P.A., Schulz G.E.
    J. Mol. Biol. 199:359-371(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiKAD1_PIG
AccessioniPrimary (citable) accession number: P00571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.