P00570 (KAD1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate kinase isoenzyme 1
Short name=AK 1
EC=2.7.4.3

Alternative name(s):
ATP-AMP transphosphorylase 1
Myokinase

Gene names

Name:

AK1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Adenylate kinase isoenzyme 1		PRO_0000158909
Regions
	Nucleotide binding	15 – 23	9	ATP By similarity
	Nucleotide binding	94 – 101	8	AMP By similarity
Sites
	Binding site	39	1	AMP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine
Experimental info
	Sequence conflict	8	1	T → A AA sequence Ref.1
	Sequence conflict	85	1	D → N AA sequence Ref.1
	Sequence conflict	98	1	E → Q AA sequence Ref.1
	Sequence conflict	126	1	T → Q AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00570 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 8F6EA93FBE3D3798	FASTA	194	21,664
	10 20 30 40 50 60 MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG SARGKMLSEI 70 80 90 100 110 120 MEKGQLVPLE TVLDMLRDAM VAKVDTSKGF LIDGYPREVQ QGEEFERRIA QPTLLLYVDA 130 140 150 160 170 180 GPETMTKRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD 190 NVFSQVCTHL DALK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Studies on adenosine triphosphate transphosphorylases. Amino acid sequence of rabbit muscle ATP-AMP transphosphorylase." Kuby S.A., Palmieri R.H., Frischat A., Fischer A.H., Wu L.H., Maland L., Manship M. Biochemistry 23:2393-2399(1984) [PubMed: 6089869] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020951 mRNA. Translation: AAX08968.1. BC114796 mRNA. Translation: AAI14797.1.
IPI	IPI00905914.
PIR	KIBOA. A00681.
RefSeq	NP_001013600.1. NM_001013582.1.
UniGene	Bt.4224.
3D structure databases
ProteinModelPortal	P00570.
SMR	P00570. Positions 1-194.
ModBase	Search...
Protein-protein interaction databases
STRING	P00570.
Proteomic databases
PRIDE	P00570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000054038; ENSBTAP00000050234; ENSBTAG00000006305.
GeneID	280715.
KEGG	bta:280715.
Organism-specific databases
CTD	203.
Phylogenomic databases
eggNOG	maNOG16709.
GeneTree	ENSGT00390000016215.
HOVERGEN	HBG108060.
PhylomeDB	P00570.
Enzyme and pathway databases
BRENDA	2.7.4.3. 908.
Family and domain databases
InterPro	IPR000850. Adenylate_kin. IPR006267. Adenylate_kin1. [Graphical view]
KO	K00939.
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
TIGRFAMs	TIGR01360. Aden_kin_iso1. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAD1_BOVIN

Accession

Primary (citable) accession number: P00570
Secondary accession number(s): Q5E9G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 27, 2006
Last modified:	November 16, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program