P00567 (KCRB_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Creatine kinase B-type
EC=2.7.3.2

Alternative name(s):
B-CK
Creatine kinase B chain

Gene names

Name:

CKB

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Nitration Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from sequence or structural similarity. Source: AgBase cellular chloride ion homeostasis Inferred from sequence or structural similarity. Source: AgBase
Cellular_component	mitochondrion Inferred from sequence or structural similarity. Source: AgBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 381	380	Creatine kinase B-type		PRO_0000211969
Regions
	Domain	11 – 98	88	Phosphagen kinase N-terminal
	Domain	125 – 367	243	Phosphagen kinase C-terminal
	Nucleotide binding	128 – 132	5	ATP By similarity
	Nucleotide binding	320 – 325	6	ATP By similarity
Sites
	Binding site	130	1	ATP By similarity
	Binding site	132	1	ATP By similarity
	Binding site	191	1	ATP By similarity
	Binding site	232	1	Substrate By similarity
	Binding site	236	1	ATP By similarity
	Binding site	285	1	Substrate By similarity
	Binding site	292	1	ATP By similarity
	Binding site	320	1	ATP By similarity
	Binding site	335	1	ATP By similarity
Amino acid modifications
	Modified residue	4	1	Phosphoserine By similarity
	Modified residue	35	1	Phosphothreonine By similarity
	Modified residue	125	1	Phosphotyrosine By similarity
	Modified residue	199	1	Phosphoserine By similarity
	Modified residue	269	1	Nitrated tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00567 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 0001F0FF3A1F3F40	FASTA	381	42,663
	10 20 30 40 50 60 MPFSNTHNTL KLRFPAEDEF PDLSAHNNHM AKVLTPEMDA ELRAKSTPSG FTLDDVIQTG 70 80 90 100 110 120 VDNPGHPFIM TVGCVAGDEE SYEAFKELFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD 130 140 150 160 170 180 LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAVEKLAVE ALSSLDGDLA GRYYALKSMT 190 200 210 220 230 240 EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM 250 260 270 280 290 300 QKGGNMKEVF TRFCNGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 310 320 330 340 350 360 HLGQHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL 370 380 IEMEQRLEQG QAIDDLMPAQ K

References

[1]	"Two tissue-specific isozymes of creatine kinase have closely matched amino acid sequences." Pickering L., Pang H., Biemann K., Munro H., Schimmel P. Proc. Natl. Acad. Sci. U.S.A. 82:2310-2314(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11306 mRNA. Translation: AAA31201.1.
PIR	KIRBCB. A00678.
RefSeq	NP_001075730.1. NM_001082261.1.
UniGene	Ocu.3366.
3D structure databases
ProteinModelPortal	P00567.
SMR	P00567. Positions 2-381.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000007616.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009085.
Organism-specific databases
CTD	1152.
Phylogenomic databases
eggNOG	COG3869.
HOGENOM	HOG000232165.
HOVERGEN	HBG001339.
Family and domain databases
Gene3D	1.10.135.10. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR022415. ATP-guanido_PTrfase_AS. IPR022414. ATP-guanido_PTrfase_cat. IPR022413. ATP-guanido_PTrfase_N. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
SUPFAM	SSF48034. SSF48034. 1 hit.
PROSITE	PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRB_RABIT

Accession

Primary (citable) accession number: P00567

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 13, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program