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P00564

- KCRM_RAT

UniProt

P00564 - KCRM_RAT

Protein

Creatine kinase M-type

Gene

Ckm

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei191 – 1911ATPPROSITE-ProRule annotation
    Binding sitei236 – 2361ATPPROSITE-ProRule annotation
    Binding sitei292 – 2921ATPPROSITE-ProRule annotation
    Binding sitei335 – 3351ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi128 – 1325ATPPROSITE-ProRule annotation
    Nucleotide bindingi320 – 3256ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: RGD

    GO - Biological processi

    1. phosphocreatine biosynthetic process Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_212547. Creatine metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase M-type (EC:2.7.3.2)
    Alternative name(s):
    Creatine kinase M chain
    M-CK
    Gene namesi
    Name:Ckm
    Synonyms:Ckmm
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2358. Ckm.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 381380Creatine kinase M-typePRO_0000211979Add
    BLAST

    Proteomic databases

    PaxDbiP00564.
    PRIDEiP00564.

    PTM databases

    PhosphoSiteiP00564.

    Expressioni

    Gene expression databases

    GenevestigatoriP00564.

    Interactioni

    Subunit structurei

    Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

    Protein-protein interaction databases

    BioGridi246449. 1 interaction.
    STRINGi10116.ENSRNOP00000022895.

    Structurei

    3D structure databases

    ProteinModelPortaliP00564.
    SMRiP00564. Positions 2-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 9888Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini125 – 367243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3869.
    GeneTreeiENSGT00550000074561.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.
    InParanoidiP00564.
    KOiK00933.
    OrthoDBiEOG7XM2XW.
    PhylomeDBiP00564.
    TreeFamiTF314214.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00564-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFGNTHNKF KLNYKPQEEY PDLSKHNNHM AKVLTPDLYN KLRDKETPSG    50
    FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGY 100
    KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE 150
    RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EQEQQQLIDD HFLFDKPVSP 200
    LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF 250
    RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTG LRGGVHVKLA 300
    NLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GAVFDISNAD RLGSSEVEQV 350
    QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K 381
    Length:381
    Mass (Da):43,045
    Last modified:January 23, 2007 - v2
    Checksum:iB573FEB1D2A41056
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161P → S in AAA40935. (PubMed:6209281)Curated
    Sequence conflicti87 – 871D → N in AAA40936. 1 PublicationCurated
    Sequence conflicti100 – 1001Y → F in AAA40935. (PubMed:6209281)Curated
    Sequence conflicti100 – 1001Y → F in AAA40936. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10140 mRNA. Translation: AAA40935.1.
    BC062058 mRNA. Translation: AAH62058.1.
    M14864 mRNA. Translation: AAA40936.1.
    PIRiA00674. KIRTCM.
    RefSeqiNP_036662.1. NM_012530.2.
    UniGeneiRn.10756.

    Genome annotation databases

    EnsembliENSRNOT00000022895; ENSRNOP00000022895; ENSRNOG00000016837.
    GeneIDi24265.
    KEGGirno:24265.
    UCSCiRGD:2358. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10140 mRNA. Translation: AAA40935.1 .
    BC062058 mRNA. Translation: AAH62058.1 .
    M14864 mRNA. Translation: AAA40936.1 .
    PIRi A00674. KIRTCM.
    RefSeqi NP_036662.1. NM_012530.2.
    UniGenei Rn.10756.

    3D structure databases

    ProteinModelPortali P00564.
    SMRi P00564. Positions 2-381.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246449. 1 interaction.
    STRINGi 10116.ENSRNOP00000022895.

    Chemistry

    ChEMBLi CHEMBL2176800.

    PTM databases

    PhosphoSitei P00564.

    Proteomic databases

    PaxDbi P00564.
    PRIDEi P00564.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000022895 ; ENSRNOP00000022895 ; ENSRNOG00000016837 .
    GeneIDi 24265.
    KEGGi rno:24265.
    UCSCi RGD:2358. rat.

    Organism-specific databases

    CTDi 1158.
    RGDi 2358. Ckm.

    Phylogenomic databases

    eggNOGi COG3869.
    GeneTreei ENSGT00550000074561.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.
    InParanoidi P00564.
    KOi K00933.
    OrthoDBi EOG7XM2XW.
    PhylomeDBi P00564.
    TreeFami TF314214.

    Enzyme and pathway databases

    Reactomei REACT_212547. Creatine metabolism.

    Miscellaneous databases

    NextBioi 602819.

    Gene expression databases

    Genevestigatori P00564.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase."
      Benfield P.A., Zivin R.A., Miller L.S., Sowder R., Smythers G.W., Henderson L., Oroszlan S., Pearson M.L.
      J. Biol. Chem. 259:14979-14984(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Fischer 344.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    3. "The nucleotide sequence of rat muscle creatine kinase cDNA and ckm transcription during myogenesis in an RNA polymerase II mutant of L6 myoblasts."
      Benfield P.A., Zivin R.A., Shearman C.W., Graf D., Henderson L., Oroszlan S., Pearson M.L.
      Exp. Biol. Med. 9:187-194(1984)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 87-381.
    4. Lubec G., Afjehi-Sadat L.
      Submitted (DEC-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 87-96; 139-148; 157-170 AND 321-341, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiKCRM_RAT
    AccessioniPrimary (citable) accession number: P00564
    Secondary accession number(s): Q6P6R9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3