Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoglycerate kinase 1

Gene

PGK1

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.By similarity

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (LOC100054877), Glyceraldehyde-3-phosphate dehydrogenase (GAPDHS)
  2. Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase 1 (PGK1)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKLR)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei313ATP; via carbonyl oxygenBy similarity1
Binding sitei344ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • phosphoglycerate kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP00559.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1 (EC:2.7.2.3)
Gene namesi
Name:PGK1
Synonyms:PGK
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity1 Publication
ChainiPRO_00001458292 – 417Phosphoglycerate kinase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysine; alternateBy similarity1
Modified residuei131N6-malonyllysine; alternateBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei203PhosphoserineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00559.
PeptideAtlasiP00559.
PRIDEiP00559.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000012929.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PGKX-ray3.00A-[»]
ProteinModelPortaliP00559.
SMRiP00559.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00559.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 186Globular domain-1Add BLAST185
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4
Regioni187 – 190Linker4
Regioni191 – 417Globular domain-2Add BLAST227
Regioni406 – 417Associated with globular domain 1Add BLAST12

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP00559.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSNKLTLD KLNVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDVG PMPDKYSLQP VAVELKSLLG KDVLFLKDCV
110 120 130 140 150
GPEVEKACAD PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIETF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA
210 220 230 240 250
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGTSLFDE EGAKIVKNLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
310 320 330 340 350
ATVASGIPAG WMGLDCGTES SKKYAEAVAR AKQIVWNGPV GVFEWEAFAR
360 370 380 390 400
GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VDALSNV
Length:417
Mass (Da):44,603
Last modified:January 23, 2007 - v2
Checksum:i5110E246AF96931A
GO

Sequence databases

PIRiA92292. KIHOG.

Cross-referencesi

Sequence databases

PIRiA92292. KIHOG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PGKX-ray3.00A-[»]
ProteinModelPortaliP00559.
SMRiP00559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000012929.

Proteomic databases

PaxDbiP00559.
PeptideAtlasiP00559.
PRIDEiP00559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP00559.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
SABIO-RKP00559.

Miscellaneous databases

EvolutionaryTraceiP00559.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK1_HORSE
AccessioniPrimary (citable) accession number: P00559
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.