Reviewed,
UniProtKB/Swiss-Prot P00559 (PGK1_HORSE)
Last modified
October 13, 2009.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phosphoglycerate kinase 1 EC=2.7.2.3 | ||||
| Gene names |
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| Organism | Equus caballus (Horse) | ||||
| Taxonomic identifier | 9796 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus |
Protein attributes
| Sequence length | 417 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. |
| Pathway | Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. |
| Subunit structure | Monomer. |
| Subcellular location | |
| Sequence similarities | Belongs to the phosphoglycerate kinase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| PTM | Acetylation Phosphoprotein |
| Technical term | 3D-structure Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Ref.2Non-traceable author statement. Source: UniProtKB |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Ref.2 Inferred from direct assay. Source: UniProtKB phosphoglycerate kinase activity Ref.2Non-traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.1 | ||||||
| Chain | 2 – 417 | 416 | Phosphoglycerate kinase 1 | PRO_0000145829 | |||||
Regions | |||||||||
| Region | 2 – 186 | 185 | Globular domain-1 | ||||||
| Region | 24 – 26 | 3 | Substrate binding By similarity | ||||||
| Region | 63 – 66 | 4 | Substrate binding By similarity | ||||||
| Region | 187 – 190 | 4 | Linker | ||||||
| Region | 191 – 417 | 227 | Globular domain-2 | ||||||
| Region | 406 – 417 | 12 | Associated with globular domain 1 | ||||||
Sites | |||||||||
| Binding site | 39 | 1 | Substrate By similarity | ||||||
| Binding site | 123 | 1 | Substrate By similarity | ||||||
| Binding site | 171 | 1 | Substrate By similarity | ||||||
| Binding site | 220 | 1 | ATP By similarity | ||||||
| Binding site | 313 | 1 | ATP; via carbonyl oxygen By similarity | ||||||
| Binding site | 344 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine | ||||||
| Modified residue | 11 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 30 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 48 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 75 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 76 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 86 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 97 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 131 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 136 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 146 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 196 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 199 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 203 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 243 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 267 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 291 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 323 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 390 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 406 | 1 | N6-acetyllysine By similarity | ||||||
Sequences
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References
| [1] | "Primary structure of 3-phosphoglycerate kinase from horse muscle. II. Amino acid sequence of cyanogen bromide peptides CB1-CB4 and CB6-CB14, sequence of methionine-containing regions, and complete sequence of the enzyme." Merrett M. J. Biol. Chem. 256:10293-10305(1981) [PubMed: 7287713] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-417. Tissue: Muscle. |
| [2] | "Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme." Banks R.D., Blake C.C.F., Evans P.R., Haser R., Rice D.W., Hardy G.W., Merrett M., Phillips A.W. Nature 279:773-777(1979) [PubMed: 450128] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). Tissue: Muscle. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PIR | KIHOG. A92292. | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| SMR | P00559. Positions 5-417. | ||||||||||||
| ModBase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSECAT00000016029; ENSECAP00000012929; ENSECAG00000014984; Equus caballus. [Genome view] | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOVERGEN | P00559. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 2.7.2.3. 1464. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit. | ||||||||||||
| PANTHER | PTHR11406. PGK. 1 hit. | ||||||||||||
| Pfam | PF00162. PGK. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00477. PHGLYCKINASE. | ||||||||||||
| PROSITE | PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | PGK1_HORSE | ||||||||
| Accession | Primary (citable) accession number: P00559 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


