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Protein

Aminoglycoside 3'-phosphotransferase

Gene

neo

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Proton acceptor1 Publication
Metal bindingi195 – 1951Magnesium
Metal bindingi208 – 2081Magnesium

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-2984-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')-II
Short name:
APH(3')II
Kanamycin kinase, type II
Neomycin-kanamycin phosphotransferase type II
Gene namesi
Name:neo
Synonyms:kan, nptII
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Aminoglycoside 3'-phosphotransferasePRO_0000204804Add
BLAST

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 153Combined sources
Turni16 – 216Combined sources
Beta strandi23 – 264Combined sources
Beta strandi35 – 406Combined sources
Beta strandi47 – 526Combined sources
Helixi60 – 7112Combined sources
Turni72 – 743Combined sources
Beta strandi80 – 856Combined sources
Beta strandi90 – 956Combined sources
Beta strandi98 – 1014Combined sources
Turni102 – 1043Combined sources
Helixi109 – 12315Combined sources
Helixi128 – 1303Combined sources
Helixi137 – 14913Combined sources
Helixi160 – 1623Combined sources
Helixi167 – 17610Combined sources
Beta strandi184 – 1874Combined sources
Helixi193 – 1953Combined sources
Beta strandi196 – 1994Combined sources
Beta strandi202 – 2065Combined sources
Beta strandi213 – 2164Combined sources
Helixi218 – 23215Combined sources
Helixi234 – 24411Combined sources
Helixi251 – 26010Combined sources
Helixi261 – 2633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ND4X-ray2.10A/B1-264[»]
ProteinModelPortaliP00552.
SMRiP00552. Positions 10-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00552.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19300.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P00552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK
60 70 80 90 100
TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
110 120 130 140 150
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA
160 170 180 190 200
GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
210 220 230 240 250
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD
260
SQRIAFYRLL DEFF
Length:264
Mass (Da):29,048
Last modified:July 21, 1986 - v1
Checksum:iCB0507C1C30863BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00004 Unassigned DNA. Translation: AAA73390.1.
V00618 Genomic DNA. Translation: CAA23892.1.
U32991 Genomic DNA. Translation: AAA85506.1.
X01702 Genomic DNA. Translation: CAA25852.1.
U66885 Genomic DNA. Translation: AAC48873.1.
PIRiA00663. PKECT5.

Genome annotation databases

KEGGiag:CAA23892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00004 Unassigned DNA. Translation: AAA73390.1.
V00618 Genomic DNA. Translation: CAA23892.1.
U32991 Genomic DNA. Translation: AAA85506.1.
X01702 Genomic DNA. Translation: CAA25852.1.
U66885 Genomic DNA. Translation: AAC48873.1.
PIRiA00663. PKECT5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ND4X-ray2.10A/B1-264[»]
ProteinModelPortaliP00552.
SMRiP00552. Positions 10-264.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA23892.

Phylogenomic databases

KOiK19300.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-2984-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP00552.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKKA2_KLEPN
AccessioniPrimary (citable) accession number: P00552
Secondary accession number(s): Q6LCR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 11, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is encoded by the kanamycin and neomycin resistance transposon Tn5. Tn5 was originally isolated from K.pneumoniae, but has been transferred to a number of bacteria including E.coli.

Keywords - Technical termi

3D-structure, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.