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Protein

Aminoglycoside 3'-phosphotransferase

Gene

neo

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190Proton acceptor1 Publication1
Metal bindingi195Magnesium1
Metal bindingi208Magnesium1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')-II
Short name:
APH(3')II
Kanamycin kinase, type II
Neomycin-kanamycin phosphotransferase type II
Gene namesi
Name:neo
Synonyms:kan, nptII
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048041 – 264Aminoglycoside 3'-phosphotransferaseAdd BLAST264

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Turni16 – 21Combined sources6
Beta strandi23 – 26Combined sources4
Beta strandi35 – 40Combined sources6
Beta strandi47 – 52Combined sources6
Helixi60 – 71Combined sources12
Turni72 – 74Combined sources3
Beta strandi80 – 85Combined sources6
Beta strandi90 – 95Combined sources6
Beta strandi98 – 101Combined sources4
Turni102 – 104Combined sources3
Helixi109 – 123Combined sources15
Helixi128 – 130Combined sources3
Helixi137 – 149Combined sources13
Helixi160 – 162Combined sources3
Helixi167 – 176Combined sources10
Beta strandi184 – 187Combined sources4
Helixi193 – 195Combined sources3
Beta strandi196 – 199Combined sources4
Beta strandi202 – 206Combined sources5
Beta strandi213 – 216Combined sources4
Helixi218 – 232Combined sources15
Helixi234 – 244Combined sources11
Helixi251 – 260Combined sources10
Helixi261 – 263Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ND4X-ray2.10A/B1-264[»]
ProteinModelPortaliP00552.
SMRiP00552.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00552.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19300.

Family and domain databases

CDDicd05150. APH. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P00552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK
60 70 80 90 100
TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
110 120 130 140 150
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA
160 170 180 190 200
GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
210 220 230 240 250
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD
260
SQRIAFYRLL DEFF
Length:264
Mass (Da):29,048
Last modified:July 21, 1986 - v1
Checksum:iCB0507C1C30863BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00004 Unassigned DNA. Translation: AAA73390.1.
V00618 Genomic DNA. Translation: CAA23892.1.
U32991 Genomic DNA. Translation: AAA85506.1.
X01702 Genomic DNA. Translation: CAA25852.1.
U66885 Genomic DNA. Translation: AAC48873.1.
PIRiA00663. PKECT5.

Genome annotation databases

KEGGiag:CAA23892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00004 Unassigned DNA. Translation: AAA73390.1.
V00618 Genomic DNA. Translation: CAA23892.1.
U32991 Genomic DNA. Translation: AAA85506.1.
X01702 Genomic DNA. Translation: CAA25852.1.
U66885 Genomic DNA. Translation: AAC48873.1.
PIRiA00663. PKECT5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ND4X-ray2.10A/B1-264[»]
ProteinModelPortaliP00552.
SMRiP00552.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA23892.

Phylogenomic databases

KOiK19300.

Miscellaneous databases

EvolutionaryTraceiP00552.

Family and domain databases

CDDicd05150. APH. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKKA2_KLEPN
AccessioniPrimary (citable) accession number: P00552
Secondary accession number(s): Q6LCR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is encoded by the kanamycin and neomycin resistance transposon Tn5. Tn5 was originally isolated from K.pneumoniae, but has been transferred to a number of bacteria including E.coli.

Keywords - Technical termi

3D-structure, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.