ID   KKA1_ECOLX              Reviewed;         271 AA.
AC   P00551; Q53299; Q8GNQ0;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   27-MAR-2024, entry version 128.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')-I;
DE            Short=APH(3')I;
DE   AltName: Full=Kanamycin kinase, type I;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type I;
GN   Name=aphA1; Synonyms=nptI;
OS   Escherichia coli.
OG   Plasmid Rts1, and Plasmid pIP1518.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=Tn903;
RX   PubMed=6270337; DOI=10.1016/0022-2836(81)90438-1;
RA   Oka A., Sugisaki H., Takanami M.;
RT   "Nucleotide sequence of the kanamycin resistance transposon Tn903.";
RL   J. Mol. Biol. 147:217-226(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=X;
RX   PubMed=2823223; DOI=10.1093/nar/15.19.8111;
RA   Vakulenko S., Kalman M., Horvath B., Simoncsits A.;
RT   "The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene
RT   from E. coli.";
RL   Nucleic Acids Res. 15:8111-8111(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=Rts1;
RX   PubMed=2013575; DOI=10.1128/jb.173.8.2600-2607.1991;
RA   Mochida S., Tsuchiya H., Mori K., Kaji A.;
RT   "Three short fragments of Rts1 DNA are responsible for the temperature-
RT   sensitive growth phenotype (Tsg) of host bacteria.";
RL   J. Bacteriol. 173:2600-2607(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Press C.M., Mahaffee W.F., Roche M.M.;
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RC   STRAIN=HM69; PLASMID=pIP1518;
RX   PubMed=8381641; DOI=10.1128/aac.37.1.78;
RA   Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R.;
RT   "Overproduction of 3'-aminoglycoside phosphotransferase type I confers
RT   resistance to tobramycin in Escherichia coli.";
RL   Antimicrob. Agents Chemother. 37:78-83(1993).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RC   TRANSPOSON=Tn903;
RX   PubMed=6261245; DOI=10.1073/pnas.77.12.7176;
RA   Grindley N.D.F., Joyce C.M.;
RT   "Genetic and DNA sequence analysis of the kanamycin resistance transposon
RT   Tn903.";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271.
RC   TRANSPOSON=Tn903;
RX   PubMed=6271455; DOI=10.1101/sqb.1981.045.01.021;
RA   Grindley N.D.F., Joyce C.M.;
RT   "Analysis of the structure and function of the kanamycin-resistance
RT   transposon Tn903.";
RL   Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.
RX   PubMed=6269080; DOI=10.1093/nar/9.14.3335;
RA   Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L.,
RA   Jansz H.S.;
RT   "Construction and characterization of recombinant plasmid DNAs containing
RT   sequences of the origin of bacteriophage phi X174 DNA replication.";
RL   Nucleic Acids Res. 9:3335-3354(1981).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- MISCELLANEOUS: This enzyme is encoded by the kanamycin resistance
CC       transposon Tn903.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: PubMed:2823223 strain is not known and has been termed 'X' in
CC       this entry. {ECO:0000305}.
CC   -!- CAUTION: Was originally (Ref.4) termed APH(3')IIa whereas it is
CC       APH(3')Ia. {ECO:0000305}.
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DR   EMBL; V00359; CAA23656.1; -; Genomic_DNA.
DR   EMBL; Y00452; CAA68507.1; -; Genomic_DNA.
DR   EMBL; M65202; AAA72095.1; -; Unassigned_DNA.
DR   EMBL; AF498082; AAN59785.1; -; Genomic_DNA.
DR   EMBL; S54065; AAD13871.1; -; Genomic_DNA.
DR   EMBL; V00621; CAA23898.1; -; Genomic_DNA.
DR   EMBL; AH000949; AAA27445.1; -; Genomic_DNA.
DR   EMBL; AH000949; AAA27446.1; -; Genomic_DNA.
DR   EMBL; V00293; CAA23568.1; -; Genomic_DNA.
DR   PIR; A00662; PKECT9.
DR   RefSeq; WP_000018322.1; NZ_WVVH01000102.1.
DR   RefSeq; WP_063841683.1; NG_047429.1.
DR   RefSeq; WP_063842148.1; NG_047437.1.
DR   RefSeq; YP_001687822.1; NC_004429.1.
DR   RefSeq; YP_002891173.1; NC_012690.1.
DR   RefSeq; YP_002995708.1; NC_012886.1.
DR   RefSeq; YP_008864028.1; NC_022992.1.
DR   RefSeq; YP_008864695.1; NC_022996.1.
DR   RefSeq; YP_008995202.1; NC_023277.2.
DR   RefSeq; YP_008997405.1; NC_023289.2.
DR   AlphaFoldDB; P00551; -.
DR   SMR; P00551; -.
DR   IntAct; P00551; 2.
DR   MINT; P00551; -.
DR   ChEMBL; CHEMBL3751655; -.
DR   DrugBank; DB01172; Kanamycin.
DR   KEGG; ag:CAA23656; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   Gene3D; 3.90.1200.10; -; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding; Plasmid;
KW   Transferase; Transposable element.
FT   CHAIN           1..271
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204802"
FT   ACT_SITE        198
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   VARIANT         27
FT                   /note="K -> R (in plasmid Rts1 and strain X)"
FT   VARIANT         77
FT                   /note="E -> A (in plasmid Rts1 and strain X)"
FT   VARIANT         126
FT                   /note="V -> A (in plasmid Rts1)"
FT   VARIANT         150
FT                   /note="A -> T (in plasmid Rts1)"
FT   VARIANT         247
FT                   /note="L -> F (in plasmid Rts1)"
FT   VARIANT         249
FT                   /note="Q -> Y (in plasmid Rts1)"
FT   CONFLICT        19
FT                   /note="L -> M (in Ref. 1 and 4)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        48
FT                   /note="D -> N (in Ref. 2; CAA68507)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        120
FT                   /note="I -> N (in Ref. 2; CAA68507)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   271 AA;  30961 MW;  3B715D9BBE8031B8 CRC64;
     MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS
     VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI
     VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK
     EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS
     PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F
//